23 research outputs found
Reactor de flujo de vórtices para la adsorción y purificación de biomoléculas
Número de publicación: ES2536461 A1 (25.05.2015) También publicado como: ES2536461 B2 (01.12.2015) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P201301126 (22.11.2013)La invención consiste en la construcción de un reactor de flujo de vórtices para ser utilizado en la cromatografía de adsorción de biomoléculas en lecho expandido y elución de las mismas, partiendo de un alimento, caldo de cultivo o extracto biológico sin clarificar.Universidad de Almerí
Vortex flow reactor assessment for the purification of monoclonal antibodies from unclarified broths
The vortex flow reactor (VFR) can be used in many chemical engineering applications. This paper assesses its novel use in the purification of monoclonal antibodies from cell broth. To this end, the IgG2a antibody was purified from the unclarified fermentation broth of transgenic mouse 55/6 hybridoma cells. Visual experiments showed that the VFR worked in the laminar vortices flow regime and the vortices displaced slightly faster than the axial flow. The VFR has the advantage of creating two sorts of flows: axial flow to produce the expanded bed and an extra vortex flow to avoid channeling and stabilize the expanded bed, the hydrodynamic behavior of which is plug flow with an experimental Pèclet number higher than 20. The pH was adjusted in the untreated fermentation broth, which was directly introduced into the reactor thus reducing the number of stages. The IgG2a purification was carried out in a single device via two steps: antibody adsorption in the expanded bed and antibody elution in the settled bed using Streamline rProtein A. A thirty-fold increase in the high-purity antibody concentration was achieved at the top of the pH5 elution peak with a total recovery of 93.1% (w/w) between elution peaks pH 5 and 3
Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality
I Congresso Ibero-Americano de Bibliotecas Escolares
Actas de la primera edición del I Congreso Iberoamericano de Bibliotecas Escolares, CIBES 2015, organizado por la Universidad Carlos III de Madrid (España), la Universidad Estatal Paulista (Brasil) y el Ayuntamiento de Getafe (España). Celebrado: 21 - 23 de octubre de 2015 en la Universidad Estatal Paulista (Marília) y 26 - 28 de octubre de 2015 en la Universidad Carlos III de Madrid (Getafe)Universidad Carlos III de Madrid (España)Universidad Estatal Paulista (Brasil)Ayuntamiento de Getafe (España)Dimensiones y visiones de la biblioteca escolar en una Educación por competencias: la
necesidad de una política estratégica / Miguel Ángel Marzal. -- Getafe ciudad educadora,
lectora y escritora: Bibliotecas escolares / Lourdes Muñoz Santiuste. -- Presente y
futuro: biblioteca escolar-CREA y proyectos interdisciplinares / Rosa Piquín. -- Cultura
en información: un reto esencial de la biblioteca escolar / Mónica Baró. -- Bibliotecas
escolares de Galicia: un mundo de oportunidades a favor de la Educación / Cristina Novoa.
-- 10 años de la Red de Bibliotecas Escolares de Extremadura (REBEX) / Casildo Macías
Pereira. -- Biblioteca Escolar y uso ético de la información para una Cultura de Paz / Ana
Barrero Tíscar. -- Dinamización de la Biblioteca Escolar Plumita durante el curso escolar
2014/15 / María Antonia Cano Cañada. -- Experiencia de la creación de una biblioteca
escolar / Susana Santos Martín. -- Grupo cooperativo Bibliotecas escolares en Red-Albacete
/ José Manuel Garrido Argandoña y Eva Leal Scasso. -- La BCREA "Juan Leiva". El fomento de
la lectura desde la web social / Andrés Pulido Villar. -- Proceso de implantación de una
herramienta de autoevaluación en la red de bibliotecas escolares de Extremadura (REBEX) /
Casildo Macías Pereira. -- La biblioteca escolar: abriendo fronteras / Lorena Verónica
Cabrera Orellana. -- O programa RBE e a avaliaçao das bibliotecas escolares: melhoria,
desenvolvimiento e innovaçao / Elsa Conde. -- Profesional de Biblioteconomía y
Documentación: esencial en la plantilla de la escuela / Pilar del Campo Puerta. -- Una
mirada activa al proceso educativo desde la biblioteca escolar / María Jesús Fontela
Fernández . -- Con otra mirada "La ilustración como vehículo de comunicación y aprendizaje
en las bibliotecas escolares" / Pablo Jurado Sánchez-Galán. -- Fingertips. Recriar a
biblioteca escolar na sala de aula / Rui Alfonso Mateus. -- Hablemos de libros. Cómo
transformar una clase de literatura en una comunidad de interpretación de textos /
Francisco César Díaz Rey. -- Inclusión social de familias inmigrantes a través de un
programa de aprendizaje de la lengua castellana / Ana Carmen Tolino Fernández-Henarejos.
-- O desenvolvimento de atividades de mediação de leitura em biblioteca escolar: o caso da
biblioteca da Escola Sesc de Ensino Médio / Vagner Amaro. -- La biblioteca escolar.
Proceso de enseñanza-aprendizaje de padres a hijos / Ana Carmen Tolino Fernández-
Henarejos. -- Leo con y para los demás / Ismael Fernández Fernández, Ana María Moreno
Vicente y Ana Beatriz Vicente Pérez. -- Nanas y arrullo. Poesía a la deriva / Bernardo
Fuentes Navarrete y Carlos García-Romeral Pérez. -- Gestión y evaluación de servicios
bibliotecarios para personas con dislexia: una biblioteca escolar inclusiva desde una
perspectiva internacional / Carmen Jorge García-Reyes. -- Sueños lectores compartidos
hechos realidad: la biblioteca escolar del C.E.I.P-S.E.S-A.A “LA PAZ” de Albacete / Ana
Rosa Cabañero Tobarra, Juan Manuel Herráez, Eva Leal Scasso, María Marín Sánchez, Ana
Belén Medrano Martínez y María José Nortes Ruipérez. -- El programa biblioteca escuela en
Civican. La literatura como elemento motivador para la alfabetización informacional /
Villar Arellano Yanguas. -- La competencia digital en el diseño curricular: desde la
biblioteca al aula / Felicidad Campal García. -- O deselvomimento da pesquisa escolar por
meio da competência em informaçao / Luciane de Fátima Cavalcante Beckman y Marta Leandro
da Mata. -- Proyecto escolar de investigación documental "Te pillé leyendo" / José Manuel
Garrido Argandoña. -- Aprender com a Biblioteca Escolar: formar para as literacias / Paula
Correia y Isabel Mendinhos. -- Sucedió en el siglo XX / María Antonia Becerra Montalbán,
Ángel Bernabé Muñoz y Sofía Vaz Romero. -- El Club de lectura en la nube / Belén Benito
Blázquez y Ana Ordás García. -- Promover a leitura e a escrita na era digital:
prácticas nas bibliotecas escolares / María Raquel Ramos. -- A biblioteca escolar e o
desafío da interculturalidade: o projeto Ser + cidadao / María da Conceição Tomé. --
Cuando la competencia digital encontró a la alfabetización informacional o Mucho ruido y
pocas nueces / Felicidad Campal García. -- Hora de ler, un programa para el fomento de la
lectura en contexto educativo / Cristina Novoa. -- Hábitos de lectura para las
competencias en información y alfabetización en información en bibliotecas escolares de
Puerto Rico / Karen Denise Centeno Casillas. -- Repositorios digitales en las bibliotecas
escolares andaluzas: situación, modelos y herramientas para su creación / Dolores Olmos
Olmos y Andrés Pulido Villar. -- Trabajando las competencias clave con las aventuras de
Mozarito en Extremadura / María Teresa Carballosa González y María Esther Nieto Vidal. --
Análisis de modelos de evaluación de la web de la biblioteca escolar / Raúl Cremades
García. -- Emociónate con las historias: El bosque de las emociones e historias con mucho
teatro / Esther Luis Pérez y Ana María Peromingo Fernández. -- Biblioteca escolar de
innovación y continuación / E. María Guerrero Palacios y Silvia Mora Ramírez. -- Uso de
estándares y licencias para la creación y difusión de contenidos en las bibliotecas
escolares / José Luis Barreiro Cebey. -- La biblioteca escolar digital móvil / Javier
Fernández Delgado. -- Uso de aplicaciones móviles para el desarrollo de
la competencia lingüística. Proyecto Hansel App Gretel / Dolores Olmos Olmos. -- A memória
e a mediação segundo Vigotski / Leda Maria Araújo, Patricia Celia Santana, Sueli Bortolin
y Leticia Gorri Molina. -- Bibliotecas escolares como tema de estudo dos alunos de
graduação em blioteconomia do Instituto de Ensino Superior da FUNLEC: estado da arte /
Tiago Pereira Nocera y Rodrigo Pereira. -- Ações de mediação da leitura e da informação
em bibliotecas escolares: um olhar sobre as bibliotecas dos Colégios de Aplicação /
Tatyanne Christina Gonçalves Ferreira Valdez y Alberto Calil Júnior. -- Mediação
pedagógica numa biblioteca de escola pública em Londrina / Rovilson José da Silva, Teba
Silva Yllana y Sueli Bortolin. -- Utilização de categorias por cores em sistema de
biblioteca voltado ao público infanto-juvenil / Liliana Giusti Serra. -- Atividades de
ensino dos atos de leitura com crianças em risco social / Adriana Naomi Fukushima da Silva
y Dagoberto Buim Arena. -- Biblioteca escolar: espaço de significados entre
alunos, professores e bibliotecários / Rodrigo Barbosa Paulo, Marisa Xavier, Helen Castro
Casarin y Creuza Barbaroto. -- A Biblioteca Escolar no Contexto da Legislação e
do Processo Educativo / Eliane Lourdes da Silva Moro, Francisca Rosaline Leite Mota y
Raimundo Martins de Lima. -- O jornal impresso como fonte de informação: a importância da
formação de leitores críticos / Mariana Pícaro Cerigatto. -- Bibliotecas escolares no
estado do Rio Grande do Sul: a trajetória de realização dos fóruns gaúchos pela melhoria
das bibliotecas escolares / Eliane Lourdes da Silva Moro y Lizandra Brasil Estabel. -- O
acesso à informação dos usuários surdos na biblioteca escolar / André Luís Onório
Coneglian y Mayara Melo Santana. -- Aprendizagem coletiva de bibliotecários e a
competência de pesquisa dos docentes: o caso do Instituto Federal do Espírito Santo /
Maristela Almeida Mercandeli Rodrigues y Beatriz Quiroz Villardi. -- Biblioteca escolar:
atores, parâmetros e competências / Mavi Galante Mancera Dall´Acqua Carvalho y Claudio
Marcondes de Castro Filho. -- Estratégias de aprendizagem de escrita no
Ensino Fundamental II / Érika Christina Kohle. -- Bebês e livros: leitura nas bebetecas.
Kenia Adriana de Aquino Modesto Silva, Juliane Francischeti Martins Motoyama y Renata
Junqueira de Souza. -- Práticas alternativas para organização de acervos nos espaços de
leitura em ambientes escolares / Luciana Souza Gracioso, Ariovaldo Alves,
Débora Nascimento, Suelen Redondo, Tainara Torika Kiri de Castro, Elizabete Angelon y
Eduardo Barbosa. -- Reflexões sobre a modelagem e criação de uma Rede Virtual de Leitores
para Bibliotecas Escolares / Carla Floriana Martins y Raoni Guerra Rajão. -- Biblioteca
escolar: espaço de formação leitora? / Silvana Ferreira de Souza Balsan y Renata
Junqueira de Souza. -- “Se a Biblioteca Escolar é minha mãe, o Google é meu pai”:
representações da relação entre Biblioteca Escolar e Google no imaginário de
alunos do ensino técnico / Adriana Bogliolo Sirihal-Duarte, Maria L. Amorim Antunes y
Raquel Miranda Vilela Paiva. -- Desafios e propostas para a universalização das
bibliotecas escolares no Brasil e na Espanha / Rodrigo Pereira, Daniela Spudeit y Fernanda
de Sales. -- Bibliotecário educador: possibilidades de atuação no contexto da biblioteca
escolar / André Carlos da Silva, Valéria Martin Valls y Mariana de Paula Silva. -- Uma ONG
para Bibliotecas Escolares : estratégia para ampliar a igualdade e capacidade de acesso
e uso da informação e educação escolar de qualidade / Suelen Camilo Ferreira y Luciana de
Souza Gracioso. -- O aluno com deficência: o papel do bibliotecário na disponibilidade de
recursos acessíveis na biblioteca escolar / Adriano de Sales Coelho, Rosilene de Melo
Oliveira y Marcos Pastana Santos. -- Biblioteca digital virtual e o uso do tablete: uma
possibilidade de construção de novas práticas de leitura na escola / Barbara Cibelli da
Silva Monteagudo y Dagoberto Buim Arena. -- A importância da biblioteca na educação de
crianças de 0 a 3 anos / Yngrid Karolline Mendonça Costa y Cyntia Graziella Guizelim
Simões Girotto. -- Comportamento Informacional de adolescentes: a relação com bibliotecas
e escolas / Nelson Sebastian Silva-Jerez y Helen de Castro S. Casarin
Repositioning of the global epicentre of non-optimal cholesterol
High blood cholesterol is typically considered a feature of wealthy western countries(1,2). However, dietary and behavioural determinants of blood cholesterol are changing rapidly throughout the world(3) and countries are using lipid-lowering medications at varying rates. These changes can have distinct effects on the levels of high-density lipoprotein (HDL) cholesterol and non-HDL cholesterol, which have different effects on human health(4,5). However, the trends of HDL and non-HDL cholesterol levels over time have not been previously reported in a global analysis. Here we pooled 1,127 population-based studies that measured blood lipids in 102.6 million individuals aged 18 years and older to estimate trends from 1980 to 2018 in mean total, non-HDL and HDL cholesterol levels for 200 countries. Globally, there was little change in total or non-HDL cholesterol from 1980 to 2018. This was a net effect of increases in low- and middle-income countries, especially in east and southeast Asia, and decreases in high-income western countries, especially those in northwestern Europe, and in central and eastern Europe. As a result, countries with the highest level of non-HDL cholesterol-which is a marker of cardiovascular riskchanged from those in western Europe such as Belgium, Finland, Greenland, Iceland, Norway, Sweden, Switzerland and Malta in 1980 to those in Asia and the Pacific, such as Tokelau, Malaysia, The Philippines and Thailand. In 2017, high non-HDL cholesterol was responsible for an estimated 3.9 million (95% credible interval 3.7 million-4.2 million) worldwide deaths, half of which occurred in east, southeast and south Asia. The global repositioning of lipid-related risk, with non-optimal cholesterol shifting from a distinct feature of high-income countries in northwestern Europe, north America and Australasia to one that affects countries in east and southeast Asia and Oceania should motivate the use of population-based policies and personal interventions to improve nutrition and enhance access to treatment throughout the world.Peer reviewe
Global variations in diabetes mellitus based on fasting glucose and haemogloblin A1c
Fasting plasma glucose (FPG) and haemoglobin A1c (HbA1c) are both used to diagnose
diabetes, but may identify different people as having diabetes. We used data from 117
population-based studies and quantified, in different world regions, the prevalence of
diagnosed diabetes, and whether those who were previously undiagnosed and detected
as having diabetes in survey screening had elevated FPG, HbA1c, or both. We developed
prediction equations for estimating the probability that a person without previously
diagnosed diabetes, and at a specific level of FPG, had elevated HbA1c, and vice versa.
The age-standardised proportion of diabetes that was previously undiagnosed, and
detected in survey screening, ranged from 30% in the high-income western region to 66%
in south Asia. Among those with screen-detected diabetes with either test, the agestandardised
proportion who had elevated levels of both FPG and HbA1c was 29-39%
across regions; the remainder had discordant elevation of FPG or HbA1c. In most low- and
middle-income regions, isolated elevated HbA1c more common than isolated elevated
FPG. In these regions, the use of FPG alone may delay diabetes diagnosis and
underestimate diabetes prevalence. Our prediction equations help allocate finite
resources for measuring HbA1c to reduce the global gap in diabetes diagnosis and
surveillance.peer-reviewe
Heterogeneous contributions of change in population distribution of body mass index to change in obesity and underweight NCD Risk Factor Collaboration (NCD-RisC)
From 1985 to 2016, the prevalence of underweight decreased, and that of obesity and severe obesity increased, in most regions, with significant variation in the magnitude of these changes across regions. We investigated how much change in mean body mass index (BMI) explains changes in the prevalence of underweight, obesity, and severe obesity in different regions using data from 2896 population-based studies with 187 million participants. Changes in the prevalence of underweight and total obesity, and to a lesser extent severe obesity, are largely driven by shifts in the distribution of BMI, with smaller contributions from changes in the shape of the distribution. In East and Southeast Asia and sub-Saharan Africa, the underweight tail of the BMI distribution was left behind as the distribution shifted. There is a need for policies that address all forms of malnutrition by making healthy foods accessible and affordable, while restricting unhealthy foods through fiscal and regulatory restrictions
Worldwide trends in hypertension prevalence and progress in treatment and control from 1990 to 2019: a pooled analysis of 1201 population-representative studies with 104 million participants
Background
Hypertension can be detected at the primary health-care level and low-cost treatments can effectively control hypertension. We aimed to measure the prevalence of hypertension and progress in its detection, treatment, and control from 1990 to 2019 for 200 countries and territories.
Methods
We used data from 1990 to 2019 on people aged 30–79 years from population-representative studies with measurement of blood pressure and data on blood pressure treatment. We defined hypertension as having systolic blood pressure 140 mm Hg or greater, diastolic blood pressure 90 mm Hg or greater, or taking medication for hypertension. We applied a Bayesian hierarchical model to estimate the prevalence of hypertension and the proportion of people with hypertension who had a previous diagnosis (detection), who were taking medication for hypertension (treatment), and whose hypertension was controlled to below 140/90 mm Hg (control). The model allowed for trends over time to be non-linear and to vary by age.
Findings
The number of people aged 30–79 years with hypertension doubled from 1990 to 2019, from 331 (95% credible interval 306–359) million women and 317 (292–344) million men in 1990 to 626 (584–668) million women and 652 (604–698) million men in 2019, despite stable global age-standardised prevalence. In 2019, age-standardised hypertension prevalence was lowest in Canada and Peru for both men and women; in Taiwan, South Korea, Japan, and some countries in western Europe including Switzerland, Spain, and the UK for women; and in several low-income and middle-income countries such as Eritrea, Bangladesh, Ethiopia, and Solomon Islands for men. Hypertension prevalence surpassed 50% for women in two countries and men in nine countries, in central and eastern Europe, central Asia, Oceania, and Latin America. Globally, 59% (55–62) of women and 49% (46–52) of men with hypertension reported a previous diagnosis of hypertension in 2019, and 47% (43–51) of women and 38% (35–41) of men were treated. Control rates among people with hypertension in 2019 were 23% (20–27) for women and 18% (16–21) for men. In 2019, treatment and control rates were highest in South Korea, Canada, and Iceland (treatment >70%; control >50%), followed by the USA, Costa Rica, Germany, Portugal, and Taiwan. Treatment rates were less than 25% for women and less than 20% for men in Nepal, Indonesia, and some countries in sub-Saharan Africa and Oceania. Control rates were below 10% for women and men in these countries and for men in some countries in north Africa, central and south Asia, and eastern Europe. Treatment and control rates have improved in most countries since 1990, but we found little change in most countries in sub-Saharan Africa and Oceania. Improvements were largest in high-income countries, central Europe, and some upper-middle-income and recently high-income countries including Costa Rica, Taiwan, Kazakhstan, South Africa, Brazil, Chile, Turkey, and Iran.
Interpretation
Improvements in the detection, treatment, and control of hypertension have varied substantially across countries, with some middle-income countries now outperforming most high-income nations. The dual approach of reducing hypertension prevalence through primary prevention and enhancing its treatment and control is achievable not only in high-income countries but also in low-income and middle-income settings
A novel vortex flow reactor for the purification of B-phycoerythrin from Porphyridium cruentum
The purification of B-phycoerythrin from a concentrated extract of disrupted Porphyridium cruentum cells was carried out using a new vortex flow reactor design for protein purification. The reactor behaved as an expanded bed in the laminar vortices flow regime where the Streamline DEAE resin was expanded by the axial flow and stabilized by the vortex flow. After the broth culture was centrifuged and resuspended in the adsorption buffer, the concentrated extract of disrupted cells was directly loaded into the vortex flow reactor. The purification of B-phycoerythrin was carried out in two steps: adsorption in the expanded bed and elution from the settled bed. 142.0 mg of B-phycoerythrin was eluted representing a total recovery yield of 86.6%. Prior to B-phycoerythrin purification, the protein adsorption of the vortex flow reactor was characterized through hydrodynamic studies and a dynamic capacity measurement using a standard protein