Application of the modal superposition technique combined with analytical elastoplastic approaches to assess the fatigue crack initiation on structural components

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.Local fatigue approaches, such as, the stress-life, strain-life or energetic approaches defines a framework to estimate the fatigue crack initiation from notches of structural details. Various engineering structures, such as, bridges, wind towers, among others, are subjected to cyclic dynamic loadings which may substantially reduce the strength of these structures. Nowadays, the structural systems tend to be more complex being necessary to find computationally efficient solutions to perform their fatigue analysis, accounting for dynamic actions corresponding to long complex loading events (e.g. diversity of trains crossing a bridge), mainly if local approaches are envisaged. Thus, this paper aims at presenting and validating a generalization of a methodology based on modal superposition technique, for fatigue damage parameters evaluation, which can be applied in fatigue analysis using local approaches. This technique was applied recently in the context of fatigue crack propagation based on fracture mechanics, although it can be extended to compute the history of local notch stresses and strains at notches. A very important conclusion is that the technique can be explored for the case of local confined plasticity at notches whenever the global elastic behaviour of the component prevails. Local submodelling can be explored with this technique to avoid the necessity of large computational models. Local models are only needed to be run under linear elastic conditions for the selected modal shapes of the structure, being the local time history of fatigue damage variable computed by modal superposition for each loading event. That time history may be further post-processed for elastoplastic conditions using Neuber or Glinka's analyses. Comparisons with direct integration elastoplastic dynamic analysis confirmed the feasibility of the proposed approach.Authors acknowledge the Portuguese Foundation for Science and Technology for the funding, particularly through the iRail doctoral program and the grants PD/BD/114101/2015 and SFRH/BPD/107825/2015. Authors gratefully acknowledge the funding of SciTech – Science and Technology for Competitive and Sustainable Industries (NORTE-01-0145-FEDER-000022), R&D project co-financed by Programa Operacional Regional do Norte

Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling

Vascular calcification (VC) is the process of deposition of calcium phosphate crystals in the blood vessel wall, with a central role for vascular smooth muscle cells (VSMCs). VC is highly prevalent in chronic kidney disease (CKD) patients and thought, in part, to be induced by phosphate imbalance. The molecular mechanisms that regulate VC are not fully known. Here we propose a novel role for the mineralisation regulator Ucma/GRP (Upper zone of growth plate and Cartilage Matrix Associated protein/Gla Rich Protein) in phosphate-induced VSMC calcification. We show that Ucma/GRP is present in calcified atherosclerotic plaques and highly expressed in calcifying VSMCs in vitro. VSMCs from Ucma/GRP(-/-) mice showed increased mineralisation and expression of osteo/chondrogenic markers (BMP-2, Runx2, beta-catenin, p-SMAD1/5/8, ALP, OCN), and decreased expression of mineralisation inhibitor MGP, suggesting that Ucma/GRP is an inhibitor of mineralisation. Using BMP signalling inhibitor noggin and SMAD1/5/8 signalling inhibitor dorsomorphin we showed that Ucma/GRP is involved in inhibiting the BMP-2-SMAD1/5/8 osteo/chondrogenic signalling pathway in VSMCs treated with elevated phosphate concentrations. Additionally, we showed for the first time evidence of a direct interaction between Ucma/GRP and BMP-2. These results demonstrate an important role of Ucma/GRP in regulating osteo/chondrogenic differentiation and phosphate-induced mineralisation of VSMCs.NWO ZonMw [MKMD 40-42600-98-13007]; FCT [SFRH/BPD/70277/2010]info:eu-repo/semantics/publishedVersio

Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging

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INVENTORY OF MOSQUITOES (DIPTERA: CULICIDAE) IN CONSERVATION UNITS IN BRAZILIAN TROPICAL DRY FORESTS

In Brazil, most studies of the Culicidae family are concentrated in rainforest regions. As such, there is a lack of knowledge regarding the diversity of Culicidae in regions with different climatic and vegetational characteristics. The aim of this study was to compile an inventory of Culicidae in protected areas of the semi-arid region of the state of Minas Gerais, Brazil, in order to better understand the diversity of the family within this region. The study was conducted across four protected areas in the northern region of the state, in tropical dry forest (TDF) fragments. Sampling methods included Shannon trap and CDC light trap, as well as active collection. A total of 11,219 mosquito specimens were collected between August 2008 and July 2012, belonging to 11 genera and 45 species; 15 new records for the state of Minas Gerais were registered, as well as 26 new records for semi-arid regions within the state. The high number of new Culicidae records in this region demonstrates the importance of inventory studies for increasing the knowledge of culicid biodiversity in Minas Gerais, and in particular within semi-arid regions of the state