Development of a reliable extraction method for the recovery of total genomic DNA from woodchip colonizing biofilm involved in gas biofiltration

[Abstract] This preliminary study focused on a critical step for the characterization of microbial ecosystem involved in biofiltration. Two aspects of nucleic acid recovery were explored: (i) cell dispersion (three methods tested) and (ii) total DNA extraction (four methods tested). The objective is to select the optimal combination of desorption/extraction methods, allowing subsequent molecular investigations to be reliable. Three relevant criteria are used to assess extraction efficiency: DNA amount and purity, and subsequent amplification feasibility

Distortion correction of two-component - two-dimensional PIV using a large imaging sensor with application to measurements of a turbulent boundary layer flow at Reτ=2386Re_{\tau} = 2386

In the past decade, advances in electronics technology have made larger imaging sensors available to the experimental fluid mechanics community. These advancements have enabled the measurement of 2-component 2-dimensional (2C-2D) velocity fields using particle image velocimetry (PIV) with much higher spatial resolution than previously possible. However, due to the large size of the sensor, the lens distortion needs to be taken into account as it will now have a more significant effect on the measurement quality that must be corrected to ensure accurate high-fidelity 2C-2D velocity field measurements. In this paper, two dewarping models, a second-order rational function (R2) and a bicubic polynomial (P3) are investigated with regards to 2C-2D PIV measurements of a turbulent boundary layer (TBL) using a large imaging sensor. Two approaches are considered and compared: (i) dewarping the images prior to the PIV cross-correlation analysis and (ii) undertaking the PIV cross-correlation analysis using the original recorded distorted images then followed by using the mapping functions derived for image dewarping to provide the correct spatial location of the velocity measurement point. The results demonstrate that the use of P3 dewarping model to correct lens distortion yields better results than the R2 dewarping model. Furthermore, both approaches for the P3 dewarping model yield results which are statistically indistinguishable

Effect of streamwise domain size on the POD mode characteristics in an adverse pressure gradient turbulent boundary layer

Proper orthogonal decomposition (POD) is used to study coherent structures on measurements of wall-bounded turbulent flows. In order to apply POD in the study of turbulent boundary layers, it is important to determine the appropriate size of the flow domain to be used in POD analysis. This study uses the 2C-2D PIV measurements of an adverse pressure gradient (APG) turbulent boundary layer (TBL) with beta ~ 0 - 3.74 and Re_delta2 ~ 1720 - 23430 where Re_delta2 is the momentum thickness based Reynolds number and beta is the Clauser's pressure gradient parameter. The measurements were obtained in the Laboratoire de Mecanique des Fluides de Lille (LMFL) High-Reynolds-Number (HRN) Boundary Layer Wind Tunnel, Lille, France. Spanning over 20 delta along the streamwise direction (where delta is the boundary layer thickness in the middle of the field of view), these are appropriate to be used in the study of the extent of the large-scale motions. POD analysis on the variable domain size along the streamwise direction (delta x) suggests that the POD mode shape changes with delta x. In this particular TBL, the shape of the first mode which represents the largest scales in the fluid flow, changes until delta x is equal to or greater than 8 delta. It is also observed the smaller scales of higher-order POD modes take longer delta x to converge in the mode shape. The streamwise integral-length-scale of 6.68 delta also confirms the size of the largest scales to be around 8 delta. Therefore, for this particular TBL, a minimum streamwise domain size of 8 delta is appropriate for the analysis of large-scale motions using POD

Intense large-scale motions in zero and adverse pressure gradient turbulent boundary layers

Proper orthogonal decomposition (POD) is used to study coherent structures in wall-bounded turbulent flows. The present study uses POD in turbulent boundary layers to determine the contributions of the intense large-scale motions (LSMs) to the Reynolds stresses. This study uses the 2C-2D PIV measurements of zero pressure gradient turbulent boundary layers (ZPG-TBL) at Re_delta2 = 7750, and adverse pressure gradient turbulent boundary layer (APG-TBL) at beta=2.27 and Re_delta2=16240, where Re_delta2 is the momentum thickness based Reynolds number and beta is the Clauser's pressure gradient parameter. The measurements were obtained in the Laboratoire de Mecanique des Fluides de Lille (LMFL) High-Reynolds-Number (HRN) Boundary Layer Wind Tunnel, Lille, France. The snapshots of the flow field are segregated into those dominated by the intense and mild LSMs based on the intensity of the temporal coefficients of the first POD mode. The intense LSMs are further decomposed into high-momentum (HM) and low-momentum (LM) motions. The relative contributions of the HM motions to the Reynolds stresses are larger near the wall as compared to the LM motions. At the wall-normal distance of the displacement thickness (delta_1), HM and LM motions have similar contributions. Beyond delta_1, the LM motions have larger contributions with their peaks located closer to the displacement thickness height. This shows that in the presence of an APG, the turbulence activity is shifted closer to the displacement thickness height

Investigation of Large Scale Motions in Zero and Adverse Pressure Gradient Turbulent Boundary Layers Using High-Spatial-Resolution PIV

Particle image velocimetry (PIV) has been used to capture the high-spatial-resolution (HSR) two-component, two-dimensional (2C-2D) velocity fields of a zero-pressure-gradient (ZPG) turbulent boundary layer (TBL) and of an adverse-pressure-gradient (APG) TBL. Proper Orthogonal Decomposition (POD) is performed on the measured velocity fields to characterize the velocity fields as large or small scale motions (LSMs or SSMs), with further characterisation of the LSMs into high and low momentum events. This paper reports the findings of the PIV experiment and the subsequent analysis of the high Reynolds number ZPG and APG TBLs

Analysis of the Contribution of Large Scale Motions to the Skin Friction of a Zero-Pressure-Gradient Turbulent Boundary Layer Using the Renard-Deck Decomposition

Coherent flow structures in turbulent boundary layers have been an active field of research for many decades, as they might be the key to reveal the mechanics of turbulence production and transport in turbulent shear flows. Renard and Deck (2016) proposed a theoretical decomposition for the mean skin-friction coefficient based on the mean kinetic energy budget in the streamwise direction. This decomposition, referred to as the Renard-Deck (RD) decomposition, decomposes the mean skin friction generation into three physical mechanisms in an absolute reference frame, namely, direct viscous dissipation, turbulent kinetic energy production, and spatial growth. In this study, the large scale motions (LSMs) are extracted using a proper orthogonal decomposition (POD) of the velocity field based on high-spatial-resolution two-dimensional - two-component particle image velocimetry (HSR 2C-2D PIV) of a zero-pressure-gradient turbulent boundary layer (ZPG-TBL), and their effect on the skin friction via RD decomposition

Exploring Attitudes Toward “Sugar Relationships” Across 87 Countries: A Global Perspective on Exchanges of Resources for Sex and Companionship

The current study investigates attitudes toward one form of sex for resources: the so-called sugar relationships, which often involve exchanges of resources for sex and/or companionship. The present study examined associations among attitudes toward sugar relationships and relevant variables (e.g., sex, sociosexuality, gender inequality, parasitic exposure) in 69,924 participants across 87 countries. Two self-report measures of Acceptance of Sugar Relationships (ASR) developed for younger companion providers (ASR-YWMS) and older resource providers (ASR-OMWS) were translated into 37 languages. We tested cross-sex and cross-linguistic construct equivalence, cross-cultural invariance in sex differences, and the importance of the hypothetical predictors of ASR. Both measures showed adequate psychometric properties in all languages (except the Persian version of ASR-YWMS). Results partially supported our hypotheses and were consistent with previous theoretical considerations and empirical evidence on human mating. For example, at the individual level, sociosexual orientation, traditional gender roles, and pathogen prevalence were significant predictors of both ASR-YWMS and ASR-OMWS. At the country level, gender inequality and parasite stress positively predicted the ASR-YWMS. However, being a woman negatively predicted the ASR-OMWS, but positively predicted the ASR-YWMS. At country-level, ingroup favoritism and parasite stress positively predicted the ASR-OMWS. Furthermore, significant cross-subregional differences were found in the openness to sugar relationships (both ASR-YWMS and ASR-OMWS scores) across subregions. Finally, significant differences were found between ASR-YWMS and ASR-OMWS when compared in each subregion. The ASR-YWMS was significantly higher than the ASR-OMWS in all subregions, except for Northern Africa and Western Asia

Validation of the Short Version (TLS-15) of the Triangular Love Scale (TLS-45) Across 37 Languages

Love is a phenomenon that occurs across the world and affects many aspects of human life, including the choice of, and process of bonding with, a romantic partner. Thus, developing a reliable and valid measure of love experiences is crucial. One of the most popular tools to quantify love is Sternberg’s 45-item Triangular Love Scale (TLS-45), which measures three love components: intimacy, passion, and commitment. However, our literature review reveals that most studies (64%) use a broad variety of shortened versions of the TLS-45. Here, aiming to achieve scientific consensus and improve the reliability, comparability, and generalizability of results across studies, we developed a short version of the scale—the TLS-15—comprised of 15 items with 5-point, rather than 9-point, response scales. In Study 1 (N = 7,332), we re-analyzed secondary data from a large-scale multinational study that validated the original TLS-45 to establish whether the scale could be truncated. In Study 2 (N = 307), we provided evidence for the three-factor structure of the TLS-15 and its reliability. Study 3 (N = 413) confirmed convergent validity and test–retest stability of the TLS-15. Study 4 (N = 60,311) presented a large-scale validation across 37 linguistic versions of the TLS-15 on a cross-cultural sample spanning every continent of the globe. The overall results provide support for the reliability, validity, and cross-cultural invariance of the TLS-15, which can be used as a measure of love components—either separately or jointly as a three-factor measure

Height and body-mass index trajectories of school-aged children and adolescents from 1985 to 2019 in 200 countries and territories: a pooled analysis of 2181 population-based studies with 65 million participants

Summary Background Comparable global data on health and nutrition of school-aged children and adolescents are scarce. We aimed to estimate age trajectories and time trends in mean height and mean body-mass index (BMI), which measures weight gain beyond what is expected from height gain, for school-aged children and adolescents. Methods For this pooled analysis, we used a database of cardiometabolic risk factors collated by the Non-Communicable Disease Risk Factor Collaboration. We applied a Bayesian hierarchical model to estimate trends from 1985 to 2019 in mean height and mean BMI in 1-year age groups for ages 5–19 years. The model allowed for non-linear changes over time in mean height and mean BMI and for non-linear changes with age of children and adolescents, including periods of rapid growth during adolescence. Findings We pooled data from 2181 population-based studies, with measurements of height and weight in 65 million participants in 200 countries and territories. In 2019, we estimated a difference of 20 cm or higher in mean height of 19-year-old adolescents between countries with the tallest populations (the Netherlands, Montenegro, Estonia, and Bosnia and Herzegovina for boys; and the Netherlands, Montenegro, Denmark, and Iceland for girls) and those with the shortest populations (Timor-Leste, Laos, Solomon Islands, and Papua New Guinea for boys; and Guatemala, Bangladesh, Nepal, and Timor-Leste for girls). In the same year, the difference between the highest mean BMI (in Pacific island countries, Kuwait, Bahrain, The Bahamas, Chile, the USA, and New Zealand for both boys and girls and in South Africa for girls) and lowest mean BMI (in India, Bangladesh, Timor-Leste, Ethiopia, and Chad for boys and girls; and in Japan and Romania for girls) was approximately 9–10 kg/m2. In some countries, children aged 5 years started with healthier height or BMI than the global median and, in some cases, as healthy as the best performing countries, but they became progressively less healthy compared with their comparators as they grew older by not growing as tall (eg, boys in Austria and Barbados, and girls in Belgium and Puerto Rico) or gaining too much weight for their height (eg, girls and boys in Kuwait, Bahrain, Fiji, Jamaica, and Mexico; and girls in South Africa and New Zealand). In other countries, growing children overtook the height of their comparators (eg, Latvia, Czech Republic, Morocco, and Iran) or curbed their weight gain (eg, Italy, France, and Croatia) in late childhood and adolescence. When changes in both height and BMI were considered, girls in South Korea, Vietnam, Saudi Arabia, Turkey, and some central Asian countries (eg, Armenia and Azerbaijan), and boys in central and western Europe (eg, Portugal, Denmark, Poland, and Montenegro) had the healthiest changes in anthropometric status over the past 3·5 decades because, compared with children and adolescents in other countries, they had a much larger gain in height than they did in BMI. The unhealthiest changes—gaining too little height, too much weight for their height compared with children in other countries, or both—occurred in many countries in sub-Saharan Africa, New Zealand, and the USA for boys and girls; in Malaysia and some Pacific island nations for boys; and in Mexico for girls. Interpretation The height and BMI trajectories over age and time of school-aged children and adolescents are highly variable across countries, which indicates heterogeneous nutritional quality and lifelong health advantages and risks

Rising rural body-mass index is the main driver of the global obesity epidemic in adults

Body-mass index (BMI) has increased steadily in most countries in parallel with a rise in the proportion of the population who live in cities(.)(1,2) This has led to a widely reported view that urbanization is one of the most important drivers of the global rise in obesity(3-6). Here we use 2,009 population-based studies, with measurements of height and weight in more than 112 million adults, to report national, regional and global trends in mean BMI segregated by place of residence (a rural or urban area) from 1985 to 2017. We show that, contrary to the dominant paradigm, more than 55% of the global rise in mean BMI from 1985 to 2017-and more than 80% in some low- and middle-income regions-was due to increases in BMI in rural areas. This large contribution stems from the fact that, with the exception of women in sub-Saharan Africa, BMI is increasing at the same rate or faster in rural areas than in cities in low- and middle-income regions. These trends have in turn resulted in a closing-and in some countries reversal-of the gap in BMI between urban and rural areas in low- and middle-income countries, especially for women. In high-income and industrialized countries, we noted a persistently higher rural BMI, especially for women. There is an urgent need for an integrated approach to rural nutrition that enhances financial and physical access to healthy foods, to avoid replacing the rural undernutrition disadvantage in poor countries with a more general malnutrition disadvantage that entails excessive consumption of low-quality calories.Peer reviewe