296 research outputs found
Supernova Remnants in the Fossil Starburst in M82
We report the discovery of ten compact H-alpha-bright sources in the
post-starburst region northeast of the center of M82, ``M82 B.'' These objects
have H alpha luminosities and sizes consistent with Type II supernova remnants
(SNRs). They fall on the same H alpha surface brightness-diameter (Sigma-D)
relation defined by SNRs in other nearby star-forming galaxies, with the M82
candidates lying preferentially at the small diameter end. These are the first
candidates for optically-visible SNRs in M82 outside the heavily obscured
central starburst within ~250 pc from the galactic center. If these sources are
SNRs, they set an upper limit to the end of the starburst in region ``B2,''
about 500 pc from the galaxy's core, of ~50 Myr. Region ``B1,'' about 1000 pc
from the core, lacks good SNR candidates and is evidently somewhat older. This
suggests star formation in the galaxy has propagated inward toward the
present-day intense starburst core.Comment: Re-submitted to AJ, referee's comments taken into account, 15 pages
LaTeX preprint style, 4 postscript figures; full-resolution figures available
from http://www.astro.virginia.edu/~rd7a/snrs/ Changes: minor textual changes
and orientation/axes of Fig.
Breathomics—exhaled volatile organic compound analysis to detect hepatic encephalopathy : a pilot study
he current diagnostic challenge with diagnosing hepatic encephalopathy (HE) is identifying those with minimal HE as opposed to the more clinically apparent covert/overt HE. Rifaximin, is an effective therapy but earlier identification and treatment of HE could prevent liver disease progression and hospitalization. Our pilot study aimed to analyse breath samples of patients with different HE grades, and controls, using a portable electronic (e) nose. 42 patients were enrolled; 22 with HE and 20 controls. Bedside breath samples were captured and analysed using an uvFAIMS machine (portable e-nose). West Haven criteria applied and MELD scores calculated. We classify HE patients from controls with a sensitivity and specificity of 0.88 (0.73-0.95) and 0.68 (0.51-0.81) respectively, AUROC 0.84 (0.75-0.93). Minimal HE was distinguishable from covert/overt HE with sensitivity of 0.79 and specificity of 0.5, AUROC 0.71 (0.57-0.84). This pilot study has highlighted the potential of breathomics to identify VOCs signatures in HE patients for diagnostic purposes. Importantly this was performed utilizing a non-invasive, portable bedside device and holds potential for future early HE diagnosis
UV-Optical Pixel Maps of Face-On Spiral Galaxies -- Clues for Dynamics and Star Formation Histories
UV and optical images of the face-on spiral galaxies NGC 6753 and NGC 6782
reveal regions of strong on-going star formation that are associated with
structures traced by the old stellar populations. We make NUV--(NUV-I) pixel
color-magnitude diagrams (pCMDs) that reveal plumes of pixels with strongly
varying NUV surface brightness and nearly constant I surface brightness. The
plumes correspond to sharply bounded radial ranges, with (NUV-I) at a given NUV
surface brightness being bluer at larger radii. The plumes are parallel to the
reddening vector and simple model mixtures of young and old populations, thus
neither reddening nor the fraction of the young population can produce the
observed separation between the plumes. The images, radial surface-brightness,
and color plots indicate that the separate plumes are caused by sharp declines
in the surface densities of the old populations at radii corresponding to disk
resonances. The maximum surface brightness of the NUV light remains nearly
constant with radius, while the maximum I surface brightness declines sharply
with radius. An MUV image of NGC 6782 shows emission from the nuclear ring. The
distribution of points in an (MUV-NUV) vs. (NUV-I) pixel color-color diagram is
broadly consistent with the simple mixture model, but shows a residual trend
that the bluest pixels in (MUV-NUV) are the reddest pixels in (NUV-I). This may
be due to a combination of red continuum from late-type supergiants and [SIII]
emission lines associated with HII regions in active star-forming regions. We
have shown that pixel mapping is a powerful tool for studying the distribution
and strength of on-going star formation in galaxies. Deep, multi-color imaging
can extend this to studies of extinction, and the ages and metallicities of
composite stellar populations in nearby galaxies.Comment: LaTeX with AASTeX style file, 29 pages with 12 figures (some color,
some multi-part). Accepted for publication in The Astrophysical Journa
PEG Branched Polymer for Functionalization of Nanomaterials with Ultralong Blood Circulation
Nanomaterials have been actively pursued for biological and medical
applications in recent years. Here, we report the synthesis of several new
poly(ethylene glycol) grafted branched-polymers for functionalization of
various nanomaterials including carbon nanotubes, gold nanoparticles (NP) and
gold nanorods (NRs), affording high aqueous solubility and stability for these
materials. We synthesize different surfactant polymers based upon
poly-(g-glutamic acid) (gPGA) and poly(maleic anhydride-alt-1-octadecene)
(PMHC18). We use the abundant free carboxylic acid groups of gPGA for attaching
lipophilic species such as pyrene or phospholipid, which bind to nanomaterials
via robust physisorption. Additionally, the remaining carboxylic acids on gPGA
or the amine-reactive anhydrides of PMHC18 are then PEGylated, providing
extended hydrophilic groups, affording polymeric amphiphiles. We show that
single-walled carbon nanotubes (SWNTs), Au NPs and NRs functionalized by the
polymers exhibit high stability in aqueous solutions at different pHs, at
elevated temperatures and in serum. Morever, the polymer-coated SWNTs exhibit
remarkably long blood circulation (t1/2 22.1 h) upon intravenous injection into
mice, far exceeding the previous record of 5.4 h. The ultra-long blood
circulation time suggests greatly delayed clearance of nanomaterials by the
reticuloendothelial system (RES) of mice, a highly desired property for in vivo
applications of nanomaterials, including imaging and drug delivery
Induced pseudoscalar coupling of the proton weak interaction
The induced pseudoscalar coupling is the least well known of the weak
coupling constants of the proton's charged--current interaction. Its size is
dictated by chiral symmetry arguments, and its measurement represents an
important test of quantum chromodynamics at low energies. During the past
decade a large body of new data relevant to the coupling has been
accumulated. This data includes measurements of radiative and non radiative
muon capture on targets ranging from hydrogen and few--nucleon systems to
complex nuclei. Herein the authors review the theoretical underpinnings of
, the experimental studies of , and the procedures and uncertainties
in extracting the coupling from data. Current puzzles are highlighted and
future opportunities are discussed.Comment: 58 pages, Latex, Revtex4, prepared for Reviews of Modern Physic
Control of Length and Spatial Functionality of Single-Wall Carbon Nanotube AFM Nanoprobes
Single-wall carbon nanotube (SWNT) nanofibrils were assembled onto conductive
atomic force microscopy (AFM) probes with the help of dielectrophoresis (DEP).
This process involved the application of a 10 V, 2 MHz, AC bias between a
metal-coated AFM probe and a dilute suspension of SWNTs. This exerted a
positive dielectrophoretic force onto the nanotubes that caused them to align
while precipitating out onto the probe. The gradual removal of the AFM probe
away from the SWNT suspension consolidated these nanotubes into nanofibrils
with a high degree of alignment as demonstrated with polarization Raman
experiments. By varying the pulling speed, immersion time, and concentration of
the SWNT suspension, one can tailor the diameter and thus the stiffness of
these probes. Precise length trimming of these nanofibrils was also performed
by their gradual immersion and dissolution into a liquid that strongly
interacted with nanotubes, (i.e., sodium dodecyl sulfate (SDS) solution).
Vacuum annealing these nanoprobes at temperature up to 450 degree C further
increased their stiffness and rendered them insoluble to SDS and all other
aqueous media. Regrowth of a new SWNT nanofibril from the side or at the end of
a previously grown SWNT nanofibril was also demonstrated by a repeated
dielectrophoretic assembly at the desired immersion depth. These SWNT
nanofibril-equipped AFM probes are electrically conductive and mechanically
robust for use as high-aspect-ratio electrochemical nanoprobes
Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression
Hsp70 proteins represent a family of chaperones that regulate cellular homeostasis and are required for cancer cell survival. However, their function and regulation in mitosis remain unknown. In this paper, we show that the major inducible cytoplasmic Hsp70 isoform, Hsp72, is required for assembly of a robust bipolar spindle capable of efficient chromosome congression. Mechanistically, Hsp72 associates with the K-fiber-stabilizing proteins, ch-TOG and TACC3, and promotes their interaction with each other and recruitment to spindle microtubules (MTs). Targeting of Hsp72 to the mitotic spindle is dependent on phosphorylation at Thr-66 within its nucleotide-binding domain by the Nek6 kinase. Phosphorylated Hsp72 concentrates on spindle poles and sites of MT-kinetochore attachment. A phosphomimetic Hsp72 mutant rescued defects in K-fiber assembly, ch-TOG/TACC3 recruitment and mitotic progression that also resulted from Nek6 depletion. We therefore propose that Nek6 facilitates association of Hsp72 with the mitotic spindle, where it promotes stable K-fiber assembly through recruitment of the ch-TOG-TACC3 complex
Hundreds of variants clustered in genomic loci and biological pathways affect human height
Most common human traits and diseases have a polygenic pattern of inheritance: DNA sequence variants at many genetic loci influence the phenotype. Genome-wide association (GWA) studies have identified more than 600 variants associated with human traits, but these typically explain small fractions of phenotypic variation, raising questions about the use of further studies. Here, using 183,727 individuals, we show that hundreds of genetic variants, in at least 180 loci, influence adult height, a highly heritable and classic polygenic trait. The large number of loci reveals patterns with important implications for genetic studies of common human diseases and traits. First, the 180 loci are not random, but instead are enriched for genes that are connected in biological pathways (P = 0.016) and that underlie skeletal growth defects (P < 0.001). Second, the likely causal gene is often located near the most strongly associated variant: in 13 of 21 loci containing a known skeletal growth gene, that gene was closest to the associated variant. Third, at least 19 loci have multiple independently associated variants, suggesting that allelic heterogeneity is a frequent feature of polygenic traits, that comprehensive explorations of already-discovered loci should discover additional variants and that an appreciable fraction of associated loci may have been identified. Fourth, associated variants are enriched for likely functional effects on genes, being over-represented among variants that alter amino-acid structure of proteins and expression levels of nearby genes. Our data explain approximately 10% of the phenotypic variation in height, and we estimate that unidentified common variants of similar effect sizes would increase this figure to approximately 16% of phenotypic variation (approximately 20% of heritable variation). Although additional approaches are needed to dissect the genetic architecture of polygenic human traits fully, our findings indicate that GWA studies can identify large numbers of loci that implicate biologically relevant genes and pathways.
High Performance In Vivo Near-IR (>1 {\mu}m) Imaging and Photothermal Cancer Therapy with Carbon Nanotubes
Short single-walled carbon nanotubes (SWNTs) functionalized by PEGylated
phospholipids are biologically non-toxic and long-circulating nanomaterials
with intrinsic near infrared photoluminescence (NIR PL), characteristic Raman
spectra, and strong optical absorbance in the near infrared (NIR). This work
demonstrates the first dual application of intravenously injected SWNTs as
photoluminescent agents for in vivo tumor imaging in the 1.0-1.4 {\mu}m
emission region and as NIR absorbers and heaters at 808 nm for photothermal
tumor elimination at the lowest injected dose (70 {\mu}g of SWNT/mouse,
equivalent to 3.6 mg/kg) and laser irradiation power (0.6 W/cm2) reported to
date. Ex vivo resonance Raman imaging revealed the SWNT distribution within
tumors at a high spatial resolution. Complete tumor elimination was achieved
for large numbers of photothermally treated mice without any toxic side effects
after more than six months post-treatment. Further, side-by-side experiments
were carried out to compare the performance of SWNTs and gold nanorods (AuNRs)
at an injected dose of 700 {\mu}g of AuNR/mouse (equivalent to 35 mg/kg) in NIR
photothermal ablation of tumors in vivo. Highly effective tumor elimination
with SWNTs was achieved at 10 times lower injected doses and lower irradiation
powers than for AuNRs. These results suggest there are significant benefits of
utilizing the intrinsic properties of biocompatible SWNTs for combined cancer
imaging and therapy.Comment: Nanoresearch, in pres
A multi-targeted approach to suppress tumor-promoting inflammation
Cancers harbor significant genetic heterogeneity and patterns of relapse following many therapies are due to evolved resistance to treatment. While efforts have been made to combine targeted therapies, significant levels of toxicity have stymied efforts to effectively treat cancer with multi-drug combinations using currently approved therapeutics. We discuss the relationship between tumor-promoting inflammation and cancer as part of a larger effort to develop a broad-spectrum therapeutic approach aimed at a wide range of targets to address this heterogeneity. Specifically, macrophage migration inhibitory factor, cyclooxygenase-2, transcription factor nuclear factor-κB, tumor necrosis factor alpha, inducible nitric oxide synthase, protein kinase B, and CXC chemokines are reviewed as important antiinflammatory targets while curcumin, resveratrol, epigallocatechin gallate, genistein, lycopene, and anthocyanins are reviewed as low-cost, low toxicity means by which these targets might all be reached simultaneously. Future translational work will need to assess the resulting synergies of rationally designed antiinflammatory mixtures (employing low-toxicity constituents), and then combine this with similar approaches targeting the most important pathways across the range of cancer hallmark phenotypes
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