Tumour necrosis factor production and natural killer cell activity in peripheral blood during treatment with recombinant tumour necrosis factor.

Tumour necrosis factor (TNF) has been found to be an important immunomodulator. Among other functions TNF activates natural killer (NK) cells and stimulates monocytes/macrophages in an autocrine fashion. TNF production and NK activity in peripheral blood mononuclear cells were determined in a clinical phase I study in which recombinant human (rh) TNF was administered as a continuous infusion weekly for a period of 8 weeks. Even though TNF production and NK activity were significantly reduced directly after rhTNF infusion the effect proved to be transient and most pronounced at the first rhTNF administration. One day after completion of the rhTNF infusion the peripheral cells released more TNF into the supernatant compared to TNF activity immediately before the rhTNF infusion. This effect was conspicuous in non-stimulated cultures. After repeated rhTNF infusions both stimulated and non-stimulated TNF production of the peripheral blood mononuclear cells was increased. NK cell activity was also enhanced after repeated cycles of rhTNF administration as compared to early rhTNF treatment. Thus, repeated rhTNF infusions lead to a stimulatory effect on TNF production and NK activity of peripheral blood cells

On the distribution of frequency ratios of kHz QPOs

The width (W), root mean squared amplitude (Rs) of lower and upper kHz quasi-periodic oscillations (QPOs) from accreting neutron stars vary with frequency. Similarly, the QPO frequency varies with the source count rate (S). Hence, the significance of a QPO, scaling as S x Rs^2/W^(1/2) will also depend on frequency. In addition, the significance also scales up with the square root of the integration time of the Fourier power density spectrum (T). Consequently, depending on the way data are considered, kHz QPOs may be detected only over a limited range of their frequency spans or detected predominantly at some frequencies, leading potentially to biases in the observed distributions of frequencies or frequency ratios. Although subject of much controversy, an observed clustering of QPO frequency ratios around 3/2 in Sco X-1, also seen in other sources, has been previously used as an argument supporting resonance based models of neutron star QPOs. In this paper, we measure how the statistical significance of both kHz QPOs vary with frequency for three prototype neutron star kHz QPO sources, namely 4U1636-536, 4U0614+091 and Sco X-1. As the significance of QPO detection depends on frequency, we show that in sensitivity-limited observations (as in the case of the RXTE/PCA), a simultaneous detection of both the lower and upper kHz QPOs can only be achieved over limited frequency ranges. As a result, even a uniform distribution of QPO frequencies will lead to peaks (in particular around 3/2) in the histogram of frequency ratios. This implies that the observed clustering of ratios does not provide any evidence for intrinsically preferred frequency ratios, thus weakening the case for a resonance mechanism at the origin of neutron star kHz QPOs.Comment: Accepted for publication in MNRAS, 9 pages, 9 figure

Consumo alimentar e estado nutricional de adolescentes ativos e não ativos fisicamente

O risco aumentado para o desenvolvimento de Doenças Crônicas Não Transmissíveis (DCNT), em decorrência do consumo alimentar inadequado e o sedentarismo verificados em adolescentes, tem repercutido na saúde desses indivíduos. Objetivou-se comparar o estado nutricional e o consumo alimentar de adolescentes ativos com adolescentes não ativos fisicamente, matriculados em um colégio particular do município de Limoeiro do Norte, Ceará. A amostra correspondeu a 72 alunos do 9° ano do ensino fundamental da referida instituição, os quais foram classificados em dois grupos, grupo dos estudantes ativos e grupo dos não ativos. Foi aplicado um formulário contendo dados relativos à atividade física, pessoais, socioeconômicos, antropométricos e alimentares. No grupo de estudantes ativos, verificou-se um número maior (66,7%) de indivíduos eutróficos. Quanto ao percentual de gordura corporal, observou-se que a maioria (58,3%) dos adolescentes ativos apresentou-se dentro do padrão de normalidade. Verificou-se no grupo ativo maior número (91,7%) de estudantes que não apresentaram risco cardiovascular quando comparados aos não ativos. O consumo alimentar encontrou-se inadequado em ambos os grupos de adolescentes estudados. Concluiu-se que o excesso de peso juntamente com o consumo alimentar inadequado, verificado entre os adolescentes não ativos, sugere um risco aumentado para o desenvolvimento de DCNT nesse público. ABSTRACT Food Consumption and Nutritional Status of Assets and Teens Not Physically ActiveThe increased risk for the development of Chronic Non communicable Diseases (NCDs), as a result of inadequate dietary intake and physical inactivity observed in adolescents has impacted the health of these individuals. The objective was to compare the nutritional status and food intake in adolescents with active physically active adolescents, enrolled in a private school in the municipality from Limoeiro do Norte, Ceará. The sample consisted of 72 students in the 9th grade of elementary school of that institution, which were classified into two groups, group of students and active group of non-active. A form containing data regarding the physical activity, personal, socioeconomic, anthropometric and dietary was applied. In the group of active students there was an excess (66.7%) of normal individuals. As for the percentage of body fat, it was observed that the majority (58.3%) of active adolescents was within normal limits. It was found in the active group larger number (91.7%) of students who did not have cardiovascular risk compared to non-active. Dietary intake found to be inadequate in both groups of adolescents studied. It is concluded that excess weight along with adequate food intake observed among non-active adolescents, suggests an increased risk for the development of NCDs in public

Predictive uncertainty in auditory sequence processing

Copyright © 2014 Hansen and Pearce. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms

Complex hand dexterity: a review of biomechanical methods for measuring musical performance

Complex hand dexterity is fundamental to our interactions with the physical, social, and cultural environment. Dexterity can be an expression of creativity and precision in a range of activities, including musical performance. Little is understood about complex hand dexterity or how virtuoso expertise is acquired, due to the versatility of movement combinations available to complete any given task. This has historically limited progress of the field because of difficulties in measuring movements of the hand. Recent developments in methods of motion capture and analysis mean it is now possible to explore the intricate movements of the hand and fingers. These methods allow us insights into the neurophysiological mechanisms underpinning complex hand dexterity and motor learning. They also allow investigation into the key factors that contribute to injury, recovery and functional compensation. The application of such analytical techniques within musical performance provides a multidisciplinary framework for purposeful investigation into the process of learning and skill acquisition in instrumental performance. These highly skilled manual and cognitive tasks present the ultimate achievement in complex hand dexterity. This paper will review methods of assessing instrumental performance in music, focusing specifically on biomechanical measurement and the associated technical challenges faced when measuring highly dexterous activities

On the functional overlap between complement and anti-microbial peptides

Intriguingly, activated complement and anti-microbial peptides share certain functionalities; lytic, phagocytic, and chemo-attractant activities and each may, in addition, exert cell instructive roles. Each has been shown to have distinct LPS detoxifying activity and may play a role in the development of endotoxin tolerance. In search of the origin of complement, a functional homolog of complement C₃ involved in opsonization has been identified in horseshoe crabs. Horseshoe crabs possess anti-microbial peptides able to bind to acyl chains or phosphate groups/saccharides of endotoxin, LPS. Complement activity as a whole is detectable in marine invertebrates. These are also a source of anti-microbial peptides with potential pharmaceutical applicability. Investigating the locality for the production of complement pathway proteins and their role in modulating cellular immune responses are emerging fields. The significance of local synthesis of complement components is becoming clearer from in vivo studies of parenchymatous disease involving specifically generated, complement-deficient mouse lines. Complement C₃ is a central component of complement activation. Its provision by cells of the myeloid lineage varies. Their effector functions in turn are increased in the presence of anti-microbial peptides. This may point to a potentiating range of activities, which should serve the maintenance of health but may also cause disease. Because of the therapeutic implications, this review will consider closely studies dealing with complement activation and anti-microbial peptide activity in acute inflammation (e.g., dialysis-related peritonitis, appendicitis, and ischemia)

Cross-Reactive Sensor Arrays for the Detection of Peptides in Aqueous Solution by Fluorescence Spectroscopy

A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl2(en)], [{RhCl2Cp*}2], and [{RuCl2(p-cymene)} 2] were combined with six different fluorescent dyes to build a cross-reactive sensor array. The fluorescence response of the individual sensor units was based on competitive complexation reactions between the peptide analytes and the fluorescent dyes. The collective response of the sensor array in a time-resolved fashion was used as an input for multivariate analyses. A sensor array comprised of only six metal-dye combinations was able to differentiate ten different dipeptides in buffered aqueous solution at a concentration of 50 uM. Furthermore, the cross-reactive sensor could be used to obtain information about the identity and the quantity of the pharmacologically interesting dipeptides carnosine and homocarnosine in a complex biological matrix, such as deproteinized human blood serum. The sensor array was also able to sense longer peptides, which was demonstrated by differentiating mixtures of the nonapeptide bradykinin and the decapeptide kallidin

K0S and Λ production in Pb-Pb collisions at sNN−−−−√=2.76 TeV

The ALICE measurement of K0S and Λ production at midrapidity in Pb-Pb collisions at sNN−−−√=2.76  TeV is presented. The transverse momentum (pT) spectra are shown for several collision centrality intervals and in the pT range from 0.4  GeV/c (0.6  GeV/c for Λ) to 12  GeV/c. The pT dependence of the Λ/K0S ratios exhibits maxima in the vicinity of 3  GeV/c, and the positions of the maxima shift towards higher pT with increasing collision centrality. The magnitude of these maxima increases by almost a factor of three between most peripheral and most central Pb-Pb collisions. This baryon excess at intermediate pT is not observed in pp interactions at s√=0.9  TeV and at s√=7  TeV. Qualitatively, the baryon enhancement in heavy-ion collisions is expected from radial flow. However, the measured pT spectra above 2  GeV/c progressively decouple from hydrodynamical-model calculations. For higher values of pT, models that incorporate the influence of the medium on the fragmentation and hadronization processes describe qualitatively the pT dependence of the Λ/K0S ratio

Azimuthally Differential Pion Femtoscopy in Pb-Pb Collisions at root s(NN)=2.76 TeV

We present the first azimuthally differential measurements of the pion source size relative to the second harmonic event plane in Pb-Pb collisions at a center-of-mass energy per nucleon-nucleon pair of root(NN)-N-s = 2.76 TeV. The measurements have been performed in the centrality range 0%-50% and for pion pair transverse momenta 0.2 <k(T) <0.7 GeV/c. We find that the R-side and R-out radii, which characterize the pion source size in the directions perpendicular and parallel to the pion transverse momentum, oscillate out of phase, similar to what was observed at the Relativistic Heavy Ion Collider. The final-state source eccentricity, estimated via R-side oscillations, is found to be significantly smaller than the initial-state source eccentricity, but remains positive-indicating that even after a stronger expansion in the in-plane direction, the pion source at the freeze-out is still elongated in the out-of-plane direction. The 3 + 1D hydrodynamic calculations are in qualitative agreement with observed centrality and transverse momentum R-side oscillations, but systematically underestimate the oscillation magnitude.Peer reviewe