Epidemiology and molecular typing of Trypanosoma cruzi in naturally-infected hound dogs and associated triatomine vectors in Texas, USA

Dataset associated with publicatio

Opportunistic additional testing of serologically positive dogs.

<p>Serologic, molecular, and histologic results of tissue samples opportunistically collected from <i>T</i>. <i>cruzi</i>-infected dogs.</p

Lineages of five groups of related dogs.

<p>Each dog is represented by its number, sex, date of birth, and IFA status at time of August 2013 testing. Dams Dog 62, Dog 79, and Dog 85 were from kennel A, dams Dog 33, Dog 37, Dog 38, and Dog 57 were from kennel C.</p

Serological testing.

<p>Blood samples from working dogs were tested for anti-<i>T</i>. <i>cruzi</i> antibodies using IFA and Chagas Stat-Pak. Only those samples positive on both assays were considered positive for calculation of seroprevalence. *In addition to these 55 IFA-positive dogs, there was one additional dog with an antibody titer of 160 that was not run on the Chagas Stat-Pak.</p

Results of serologic and PCR testing.

<p>Blood samples from working dogs were tested for anti-<i>Trypanosoma cruzi</i> antibodies using IFA and Chagas Stat-Pak; DNA extracted from the blood clots were tested for presence of <i>T</i>. <i>cruzi</i> DNA using two PCR assays. Samples were considered serologically positive if positive on both IFA and Chagas Stat-Pak and PCR positive if they were positive on both assays.</p