Obesogenic Lifestyle and Its Influence on Adiposity in Children and Adolescents, Evidence from Mexico

Overweight (OW) and obesity (OB) during childhood/adolescence are major public health problems in Mexico. Several obesogenic lifestyle (OL) risk factors have been identified, but the burden and consequences of them in Mexican children/adolescents remain unclear. The objective of this study was to estimate the prevalence of OL components and describe their relationships with adiposity, and OW/OB. A population-based cross-sectional study of Mexican children/adolescents with nutritional assessment, data collection on daily habits and adiposity as fat-mass index (FMI) by dual-energy X-ray absorptiometry was performed. Individual OL-components: "inactivity," "excessive screen time," "insufficient sleep," "unhealthy-diet", were defined according to non-adherence to previously published healthy recommendations. RESULTS: 1449 subjects were assessed between March 2015 to April 2018. Sixteen percent of subjects had all four OL-components, 40% had three, 35% had two, 9% had one, and 0.5% had none. A cumulative OL score showed a significant dose-response effect with FMI. The combination of inactivity, excessive screen time, and insufficient sleep showed the highest risk association to OW/OB and higher values of FMI. CONCLUSIONS: The prevalence of OL-components was extremely high and associated with increased adiposity and OW/OB. Several interventions are needed to revert this major public health threat

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Comparison of plants used for skin and stomach problems in Trinidad and Tobago with Asian ethnomedicine

RefereedThis paper provides a preliminary evaluation of fifty-eight ethnomedicinal plants used in Trinidad and Tobago for skin problems, stomach problems, pain and internal parasites for safety and possible efficacy. Thirty respondents, ten of whom were male were interviewed from September 1996 to September 2000 on medicinal plant use for health problems. The respondents were obtained by snowball sampling, and were found in thirteen different sites, 12 in Trinidad and one in Tobago. The uses are compared to those current in Asia. Bambusa vulgaris, Bidens alba, Jatropha curcas, Neurolaena lobata, Peperomia rotundifolia and Phyllanthus urinaria are possibly efficacous for stomach problems, pain and internal parasites. Further scientific study of these plants is warranted

The Ilopango caldera complex, El Salvador: Origin and early ignimbrite-forming eruptions of a graben/pull-apart caldera structure

The Ilopango caldera is located in the central part of El Salvador, within the right-lateral El Salvador Fault System (ESFZ) and adjacent to the capital city of San Salvador. The caldera has a polygonal shape of 17 × 13 km and hosts an intra-caldera lake. Ilopango caldera had multiple collapse eruptions that formed widespread and voluminous silicic ignimbrites. Volcanic activity of the caldera has been controlled by strike-slip faults of the ESFZ. In this work we present the geological characteristics of the first three ignimbrite-forming eruptions of Ilopango caldera, providing an interpretation of the origin and initial stages of the volcanic evolution of this caldera complex. An initial extensional regime of the ESFZ possibly developed a graben at or near the actual Ilopango caldera, where the graben's master faults worked as fissure vents during the first caldera collapse. The Olocuilta Ignimbrite was emplaced at 1.785 ± 0.01 Ma BP, with a Dense Rock Equivalent (DRE) volume > 50 km 3 (probably ~300 km 3 ). The ESFZ stress gradually changed from extensive to transtensive, inducing the second collapse associated with a pull-apart caldera, producing the Colima Ignimbrite at 1.56 ± 0.01 Ma BP, with a DRE volume of >11 km 3 . The transtensive regime increased along the ESFZ, producing the third collapse in the pull-apart graben caldera apparently affected by the newly formed strike-slip San Vicente Fault. This phase corresponds to the explosive eruption that formed the Apopa Ignimbrite at ~1.34 Ma BP, with >9 km 3 DRE volume. The latter ignimbrite marks a change in the eruptive style producing hydromagmatic pyroclastic flows followed by a dense ignimbrite with coignimbrite lithic breccias. These features suggest the involvement of water that could come from a paleoIlopango lake within the caldera depression associated with the second caldera collapse at 1.56 Ma BP. Ilopango is thus a multistage caldera system associated with the largest explosive events registered in El Salvador so far

The Ilopango caldera complex, El Salvador: Stratigraphic revision of the complete eruptive sequence and recurrence of large explosive eruptions

Ilopango caldera erupted episodically at least 13 tuff-forming eruptions with a minimum estimate volume of 1–5 km 3 DRE per eruption, reaching up to 150 km 3 DRE for the first caldera-forming eruption. All tuffs are of dacitic-rhyolitic composition. The complete pyroclastic sequence spans a range in time from 1.785 to 0.0015 Ma, and based on stratigraphy and geochronology constraints can be divided into three formations: the Comalapa, Altavista and Tierras Blancas formations. In this work, we focus on the members of the newly described Altavista Formation (middle part of Ilopango caldera volcanic sequence), which consist of six consolidated pyroclastic deposits or tuffs. Each tuff corresponds to a specific eruption followed by a period of quiescence during which soil beds were developed on the deposits. The ages of the Altavista Formation ranges from 918 to 257 ka, based on new 40 Ar/ 39 Ar, U/Pb-zircon, and U/Th-zircon analyses. The tuffs of this formation show similar characteristics in mineralogy and composition. They are calcalkaline, rhyodacitic tuffs, with plagioclase, clinopyroxene, and hornblende. From field mapping and descriptions of the deposits, we have inferred the eruptive styles that include pumice fallouts, pyroclastic density currents and also hydromagmatic explosions. The common vent in all tuffs was the Ilopango caldera and each member of the Altavista Formation could correspond to a caldera collapse event, except for one of the six eruptions. The volume of each member was estimated to be >30 km 3 DRE, which is the same order of magnitude than that estimated for the Tierra Blanca Joven (TBJ) eruption at about 1,500 B. P, and smaller than those of the ignimbrites of the Comalapa Formation, the first three members of the Ilopango caldera reported previously. The tuffs of the Altavista Formation are visible up to 15–20 km away from the caldera's topographic margin. The recurrence interval of large explosive events at the Ilopango caldera was established by integrating the stratigraphic and geochronologic data of all 13 ignimbrites and pumice fallouts erupted from Ilopango caldera since the first one at 1.78 Ma to the last explosive event (TBJ)

Shared Odds of <i>Borrelia</i> and Rabies Virus Exposure in Serbia

Lyme borreliosis (LB) is the most common tick-borne disease in Serbia and other European countries. Rabies is a fatal zoonosis distributed worldwide and is caused by the rabies virus. Professionals at risk of rabies-including veterinarians, hunters, communal service workers, and forestry workers-overlap with some professions at a higher risk of exposure to tick bites and tick-borne pathogen infections. We hypothesized that individuals identified by the public health system as at risk of rabies virus infection, and consequently vaccinated against rabies virus, also share a higher likelihood of Borrelia exposure. To test our hypothesis, a case-control study was carried out during 2019 in Serbia to determine the seroprevalence of anti-Borrelia antibodies in two case groups (individuals at risk and vaccinated against rabies virus) and a control group (individuals without risk of rabies). Individuals vaccinated against rabies following either "pre-exposure protocol" (PrEP, n = 58) or "post-exposure protocol" (PEP, n = 42) were considered as rabies risk groups and healthy blood donors (n = 30) as the control group. The results showed higher Borrelia seroprevalence in PrEP (17.2%; 10/58) and PEP (19.0%; 8/42) groups compared with the control group (6.67%; 2/30). Furthermore, odds ratio (OR) analysis showed that risk of rabies (in either the PrEP (OR = 2.91) or PEP (OR = 3.29) groups) is associated with increased odds of being seropositive to Borrelia. However, the difference in Borrelia seroprevalence between groups was not statistically significant (Chi-square (χ²) test p > 0.05). The shared odds of LB and rabies exposure found in this study suggest that, in countries where both diseases occur, the common citizen can be at risk of both diseases when in a risky habitat. These findings are important to guide physicians in targeting high-risk groups, and diagnose LB, and to guide decision-makers in targeting control and prevention measures for both infections in risk areas