Effectiveness of microbial inoculants from tempeh and tapai in home scale composting

This study evaluated the effectiveness and necessity of microbial inoculants on home scale composting (food waste: rice bran: dried leaves 2:1:1) with the attempt to divert food waste from the landfill. In this study, the feedstock was inoculated with three formulations of microbial inoculants (MI) and one control, namely 100% Tempeh solution, 100% Tapai solution, Effective MicroorganismTM (EM™) and water as control. Various physico-chemical properties and enzymatic activities were evaluated during the composting process. The quality of the end composts was evaluated by the physico-chemical properties, bioassays responds, characteristics of humic acid, nutrients content and pathogens content. It was found that the temperature of all three feedstock treated with MI can be heat up to higher level (>50oC) and did not produced foul odour compared to the control. However, for most of the monitored parameters of all treatments (with MI and control) during the composting process showed similar changes without significant differences. For the end composts (week 8), no significance difference was identified for the characteristics including pH (~7), EC (~3 dS/m), C: N (100%) but all indicating well matured. Nevertheless, composts with MI showed higher content of nitrogen than the control. In comparison with composts treated with EM™, MI from Tempeh produced compost with higher nitrogen and humic content; MI from Tapai showed compost with better ability to raise the temperature to a higher degree. This study concludes that MI produced from Tempeh and Tapai showed comparable performance as the commercial brand, the Effective Microorganism™ as microbial inoculants

Air-cushioning below an impacting wave-structured disk:Free-surface deformation and slamming load

Prior to the impact of a horizontal disk onto a liquid surface, the air underneath flows radially outward across the liquid surface to escape from below the edge of the disk. Such airflow causes the surface to be elevated near the disk edge, creating a free-surface condition that influences the details of the subsequent impact dynamics. In this work, the nature of the surface elevation under an impacting disk is investigated by modulating the forcing of the free surface: The airflow below the disk is altered by imposing a radially symmetric wave structure of varying wavelength on the impacting disk surface. Subsequently, the liquid surface deformation before impact is measured experimentally using a total internal reflection technique. The experiments provide convincing evidence that supports the argument that the surface elevation is an instability of the Kelvin-Helmholtz type. In addition, the impact force exerted on the wave-structured disks is measured using a load cell. Due to the macroscopic wave structure on the disk, the maximum impact force is significantly reduced, and the results indicate that both the free-surface deformation before impact and the way in which the impacting surface is subsequently wetted influence the maximum impact force.</p

Pre- and post-treatment assessment for the anaerobic digestion of lignocellulosic waste: P-graph

Lignocellulosic waste is one of the most abundant and potential feedstocks for anaerobic digestion (AD), but the energy efficiency is limited by the lignocellulosic composition which is recalcitrant to biodegradation. Pretreatment of feedstock and the post-treatment of biogas and digestate play a significant role in enhancing the AD efficiency as well as the product utilisation. This study aims to determine the cost-optimal pre-and post-treatment pathway for an AD of lignocellulosic waste by applying P-graph. The economic balance between the main operating cost, yield and quality of products were considered. The treatment options were overviewed followed by a case study considered a different combination of physical, chemical and biological pretreatments, biogas post-treatment (combine heat and power, fuel cell, biomethane, biofuel) and digestate treatments. A total of 9 pre-treatments for lignocellulosic waste, 2 digestate post-treatments and 9 post-treatments for biogas were evaluated in this study. Chemical pre-treatment by CaO, post-treatment by H2S removal with membrane separation for biomethane production and without the composting of digestate is suggested as the optimal treatment pathway for lignocellulosic waste

Locating carbon neutral mobility hubs using artificial intelligence techniques

This research proposes a novel, three-tier AI-based scheme for the allocation of carbon–neutral mobility hubs. Initially, it identified optimal sites using a genetic algorithm, which optimized travel times and achieved a high fitness value of 77,000,000. Second, it involved an Ensemble-based suitability analysis of the pinpointed locations, using factors such as land use mix, densities of population and employment, and proximities of parking, biking, and transit. Each factor is weighted by its carbon emissions contribution, then incorporated into a suitability analysis model, generating scores that guide the final selection of the most suitable mobility hub sites. The final step employs a traffic assignment model to evaluate these sites’ environmental and economic impacts. This includes measuring reductions in vehicle kilometers traveled and calculating other cost savings. Focusing on addressing sustainable development goals 11 and 9, this study leverages advanced techniques to enhance transportation planning policies. The Ensemble model demonstrated strong predictive accuracy, achieving an R-squared of 95% in training and 53% in testing. The identified hubs’ sites reduced daily vehicle travel by 771,074 km, leading to annual savings of 225.5 million USD. This comprehensive approach integrates carbon-focused analyses and post-assessment evaluations, thereby offering a comprehensive framework for sustainable mobility hub planning

An emissions analysis for environmentally sustainable freight transportation modes: distance and capacity

Transportation is one of the largest air pollutants as well as the Greenhouse Gas (GHGs) contributors. The inclusion of air pollutants in optimisation studies is less established than the use of CO2 and/or GHGs which are often used as an indicator of environmental sustainability. This study aims to assess environmentally sustainable alternatives for freight transportation by considering both GHG and air pollutants. A case study identifying air emissions of different freight transport modes for moving goods from Rotterdam is presented. The assessed freight transports include articulated lorry, container ship, bulk carrier, and rail operated by diesel. The environmentally sustainable freight transport of the studied route based on the common practice (GHGs only) and the inclusion of air pollutants are discussed. Evaluation of the results shows that transport with lower GHG emissions does not result on PM and SOx as well. A possibility of inappropriate decision making has been consequently highlighted. The impact of loaded capacity in moving the goods on the emission is assessed from the perspective of logistics service provider. A further comprehensive study which includes the entire life cycle is needed as this study only considers the tank to wheel emissions

Effect of H-NS on the elongation and compaction of single DNA molecules in a nanospace

10.1039/c3sm51214bSoft Matter9409593-960

Insulin-like growth factor - Oestradiol crosstalk and mammary gland tumourigenesis

Development and differentiation of the mammary gland are dependent on the appropriate temporal expression of both systemically acting hormones and locally produced growth factors. A large body of evidence suggests that molecular crosstalk between these hormonal and growth factor axes is crucial for appropriate cell and tissue function. Two of the most important trophic factors involved in this process are the oestrogen (E) and insulin-like growth factor (IGF) molecular axes. The reciprocal crosstalk that exists between these pathways occurs at transcriptional/post-transcriptional and translational/post-translational levels regulate the expression and activity of genes involved in this process. In a clinical context an important consequence of such crosstalk in the mammary gland is the role which it may play in the aetiology, maintenance and development of breast tumours. Although oestradiol (E2) acting through oestrogen receptors α and β (ERα/β) is important for normal mammary gland function it can also provide a mitogenic drive to ER+ breast tumours. Therefore over several years anti-oestrogen therapeutic regimens in the form of selective oestrogen receptor modulators (SERMs - e.g. tamoxifen), aromatase inhibitors (AI e.g. anastrozole) or selective oestrogen receptor down regulators (SERDs - e.g. fulvestrant) have been used in an adjuvant setting to control tumour growth. Although initial response is usually encouraging, large cohorts of patients eventually develop resistance to these treatments leading to tumour recurrence and poor prognosis. There are potentially many routes by which breast cancer (BC) cells could escape anti-oestrogen based therapeutic strategies and one of the most studied is the possible growth factor mediated activation of ER(s). Because of this, growth factor modulation of ER activity has been an intensively studied route of molecular crosstalk in the mammary gland. The insulin-like growth factors (IGF-1 and -2) are amongst the most potent mitogens for mammary epithelial cells and there is accumulating evidence that they interact with the E2 axis to regulate mitogenesis, apoptosis, adhesion, migration and differentiation of mammary epithelial cells. Such interactions are bi-directional and E2 has been shown to regulate the expression and activity of IGF axis genes with the general effect of sensitising breast epithelial cells to the actions of IGFs and insulin. In this short review we discuss the evidence for the involvement of crosstalk between the insulin-like growth factor (IGF) and oestrogen axes in the mammary gland and comment on the relevance of such studies in the aetiology and treatment of BC

Cognitive mechanisms associated with auditory sensory gating

Sensory gating is a neurophysiological measure of inhibition that is characterised by a reduction in the P50 event-related potential to a repeated identical stimulus. The objective of this work was to determine the cognitive mechanisms that relate to the neurological phenomenon of auditory sensory gating. Sixty participants underwent a battery of 10 cognitive tasks, including qualitatively different measures of attentional inhibition, working memory, and fluid intelligence. Participants additionally completed a paired-stimulus paradigm as a measure of auditory sensory gating. A correlational analysis revealed that several tasks correlated significantly with sensory gating. However once fluid intelligence and working memory were accounted for, only a measure of latent inhibition and accuracy scores on the continuous performance task showed significant sensitivity to sensory gating. We conclude that sensory gating reflects the identification of goal-irrelevant information at the encoding (input) stage and the subsequent ability to selectively attend to goal-relevant information based on that previous identification

Multiphoton Quantum Optics and Quantum State Engineering

We present a review of theoretical and experimental aspects of multiphoton quantum optics. Multiphoton processes occur and are important for many aspects of matter-radiation interactions that include the efficient ionization of atoms and molecules, and, more generally, atomic transition mechanisms; system-environment couplings and dissipative quantum dynamics; laser physics, optical parametric processes, and interferometry. A single review cannot account for all aspects of such an enormously vast subject. Here we choose to concentrate our attention on parametric processes in nonlinear media, with special emphasis on the engineering of nonclassical states of photons and atoms. We present a detailed analysis of the methods and techniques for the production of genuinely quantum multiphoton processes in nonlinear media, and the corresponding models of multiphoton effective interactions. We review existing proposals for the classification, engineering, and manipulation of nonclassical states, including Fock states, macroscopic superposition states, and multiphoton generalized coherent states. We introduce and discuss the structure of canonical multiphoton quantum optics and the associated one- and two-mode canonical multiphoton squeezed states. This framework provides a consistent multiphoton generalization of two-photon quantum optics and a consistent Hamiltonian description of multiphoton processes associated to higher-order nonlinearities. Finally, we discuss very recent advances that by combining linear and nonlinear optical devices allow to realize multiphoton entangled states of the electromnagnetic field, that are relevant for applications to efficient quantum computation, quantum teleportation, and related problems in quantum communication and information.Comment: 198 pages, 36 eps figure

Sequence determinants of breakpoint location during HIV-1 intersubtype recombination

Retroviral recombination results from strand switching, during reverse transcription, between the two copies of genomic RNA present in the virus. We analysed recombination in part of the envelope gene, between HIV-1 subtype A and D strains. After a single infection cycle, breakpoints clustered in regions corresponding to the constant portions of Env. With some exceptions, a similar distribution was observed after multiple infection cycles, and among recombinant sequences in the HIV Sequence Database. We compared the experimental data with computer simulations made using a program that only allows recombination to occur whenever an identical base is present in the aligned parental RNAs. Experimental recombination was more frequent than expected on the basis of simulated recombination when, in a region spanning 40 nt from the 5′ border of a breakpoint, no more than two discordant bases between the parental RNAs were present. When these requirements were not fulfilled, breakpoints were distributed randomly along the RNA, closer to the distribution predicted by computer simulation. A significant preference for recombination was also observed for regions containing homopolymeric stretches. These results define, for the first time, local sequence determinants for recombination between divergent HIV-1 isolates