Design of RNAi reagents for invertebrate model organisms and human disease vectors

RNAi has become an important tool to silence gene expression in a variety of organisms, in particular when classical genetic methods are missing. However, application of this method in functional studies has raised new challenges in the design of RNAi reagents in order to minimize false positive and false negative results. Since the performance of reagents can be rarely validated on a genome-wide scale, improved computational methods are required that consider experimentally derived design parameters. Here, we describe computational methods for the design of RNAi reagents for invertebrate model organisms and human disease vectors, such as Anopheles. We describe procedures on how to design short and long double-stranded RNAs for single genes, and evaluate their predicted specificity and efficiency. Using a bioinformatics pipeline we also describe how to design a genome-wide RNAi library for Anopheles gambiae

Discurso de investidura como Doctor Honoris Causa del Profesor Doctor D. Boutros-Ghali

Nombrado Doctor Honoris Causa el 15 de abril de 199

mps17-BGhali2MCB199406270

[Letter dated 27 June 1994 from Boutros Boutros-Ghali notifying M. Cherif Bassiouni that the final report of the Commission had been submitted to the Security Council]https://scholarlycommons.law.case.edu/documents_780/1042/thumbnail.jp

Log::ProgramInfo: A Perl module to collect and log data for bioinformatics pipelines.

BackgroundTo reproduce and report a bioinformatics analysis, it is important to be able to determine the environment in which a program was run. It can also be valuable when trying to debug why different executions are giving unexpectedly different results.ResultsLog::ProgramInfo is a Perl module that writes a log file at the termination of execution of the enclosing program, to document useful execution characteristics. This log file can be used to re-create the environment in order to reproduce an earlier execution. It can also be used to compare the environments of two executions to determine whether there were any differences that might affect (or explain) their operation.AvailabilityThe source is available on CPAN (Macdonald and Boutros, Log-ProgramInfo. http://search.cpan.org/~boutroslb/Log-ProgramInfo/).ConclusionUsing Log::ProgramInfo in programs creating result data for publishable research, and including the Log::ProgramInfo output log as part of the publication of that research is a valuable method to assist others to duplicate the programming environment as a precursor to validating and/or extending that research

Space-time coding techniques with bit-interleaved coded modulations for MIMO block-fading channels

The space-time bit-interleaved coded modulation (ST-BICM) is an efficient technique to obtain high diversity and coding gain on a block-fading MIMO channel. Its maximum-likelihood (ML) performance is computed under ideal interleaving conditions, which enables a global optimization taking into account channel coding. Thanks to a diversity upperbound derived from the Singleton bound, an appropriate choice of the time dimension of the space-time coding is possible, which maximizes diversity while minimizing complexity. Based on the analysis, an optimized interleaver and a set of linear precoders, called dispersive nucleo algebraic (DNA) precoders are proposed. The proposed precoders have good performance with respect to the state of the art and exist for any number of transmit antennas and any time dimension. With turbo codes, they exhibit a frame error rate which does not increase with frame length.Comment: Submitted to IEEE Trans. on Information Theory, Submission: January 2006 - First review: June 200