53 research outputs found
The role of survivin in angiogenesis during zebrafish embryonic development
<p>Abstract</p> <p>Background</p> <p>Survivin is the smallest member of the inhibitor of apoptosis (IAP) gene family. Recently, the zebrafish <it>survivin-1 </it>gene has been cloned, showing remarkable sequence identity and similarity over the BIR domain compared with human and mouse <it>survivin </it>gene. Here we investigated the role of survivin in angiogenesis during zebrafish development. Morpholinos (MOs) targeting the 5' untranslated region (UTR) (Sur<sub>UTR</sub>) and sequences flanking the initiation codon (Sur<sub>ATG</sub>) of zebrafish <it>survivin-1 </it>gene were injected into embryos at 1â4 cell stage. Vasculature was examined by microangiography and GFP expression in <it>Tg(fli1:EGFP)</it><sup><it>y</it>1 </sup>embryos. Results: In embryos co-injected with Sur<sub>UTR </sub>and Sur<sub>ATG</sub>-MOs, vasculogenesis was intact but angiogenesis was markedly perturbed, especially in the inter-segmental vessels (ISV) and dorsal longitudinal anastomotic vessels (DLAV) of the trunk, the inner optic circle and optic veins of developing eyes and the sub-intestinal vessels. Apoptosis was increased, as shown by TUNEL staining and increase in caspase-3 activity. Efficacy of Sur<sub>UTR </sub>and Sur<sub>ATG</sub>-MOs was demonstrated by translation inhibition of co-injected 5'UTR survivin:GFP plasmids. The phenotypes could be recapitulated by splice-site MO targeting the exon2-intron junction of <it>survivin </it>gene and rescued by <it>survivin </it>mRNA. Injection of human vascular endothelial growth factor (VEGF) protein induced ectopic angiogenesis and increased survivin expression, whereas treatment with a VEGF receptor inhibitor markedly reduced angiogenesis and suppressed survivin expression. Conclusion: Survivin is involved in angiogenesis during zebrafish development and may be under VEGF regulation.</p
Outgoing NearâInfrared Radiation From Vegetation Scales With Canopy Photosynthesis Across a Spectrum of Function, Structure, Physiological Capacity, and Weather
We test the relationship between canopy photosynthesis and reflected near-infrared radiation from vegetation across a range of functional (photosynthetic pathway and capacity) and structural conditions (leaf area index, fraction of green and dead leaves, canopy height, reproductive stage, and leaf angle inclination), weather conditions, and years using a network of field sites from across central California. We based our analysis on direct measurements of canopy photosynthesis, with eddy covariance, and measurements of reflected near-infrared and red radiation from vegetation, with light-emitting diode sensors. And we interpreted the observed relationships between photosynthesis and reflected near-infrared radiation using simulations based on the multilayer, biophysical model, CanVeg. Measurements of reflected near-infrared radiation were highly correlated with measurements of canopy photosynthesis on half-hourly, daily, seasonal, annual, and decadal time scales across the wide range of function and structure and weather conditions. Slopes of the regression between canopy photosynthesis and reflected near-infrared radiation were greatest for the fertilized and irrigated C4 corn crop, intermediate for the C3 tules on nutrient-rich organic soil and nitrogen fixing alfalfa, and least for the native annual grasslands and oak savanna on nutrient-poor, mineral soils. Reflected near-infrared radiation from vegetation has several advantages over other remotely sensed vegetation indices that are used to infer canopy photosynthesis; it does not saturate at high leaf area indices, it is insensitive to the presence of dead legacy vegetation, the sensors are inexpensive, and the reflectance signal is strong. Hence, information on reflected near-infrared radiation from vegetation may have utility in monitoring carbon assimilation in carbon sequestration projects or on microsatellites orbiting Earth for precision agriculture applications
A coupled ground heat flux-surface energy balance model of evaporation using thermal remote sensing observations
One of the major undetermined problems in evaporation (ET) retrieval using thermal infrared remote sensing is the lack of a physically based ground heat flux (G) model and its integration within the surface energy balance (SEB) equation. Here, we present a novel approach based on coupling a thermal inertia (TI)-based mechanistic G model with an analytical surface energy balance model, Surface Temperature Initiated Closure (STIC, version STIC1.2). The coupled model is named STIC-TI. The model is driven by noonânight (13:30 and 01:30 local time) land surface temperature, surface albedo, and a vegetation index from MODIS Aqua in conjunction with a clear-sky net radiation sub-model and ancillary meteorological information. SEB flux estimates from STIC-TI were evaluated with respect to the in situ fluxes from eddy covariance measurements in diverse ecosystems of contrasting aridity in both the Northern Hemisphere and Southern Hemisphere. Sensitivity analysis revealed substantial sensitivity of STIC-TI-derived fluxes due to the land surface temperature uncertainty. An evaluation of noontime G (Gi) estimates showed 12â%â21â% error across six flux tower sites, and a comparison between STIC-TI versus empirical G models also revealed the substantially better performance of the former. While the instantaneous noontime net radiation (RNi) and latent heat flux (LEi) were overestimated (15â% and 25â%), sensible heat flux (Hi) was underestimated (22â%). Overestimation (underestimation) of LEi (Hi) was associated with the overestimation of net available energy (RNiâGi) and use of unclosed surface energy balance flux measurements in LEi (Hi) validation. The mean percent deviations in Gi and Hi estimates were found to be strongly correlated with satellite dayânight view angle difference in parabolic and linear pattern, and a relatively weak correlation was found between dayânight view angle difference versus LEi deviation. Findings from this parameter-sparse coupled GâET model can make a valuable contribution to mapping and monitoring the spatiotemporal variability of ecosystem water stress and evaporation using noonânight thermal infrared observations from future Earth observation satellite missions such as TRISHNA, LSTM, and SBG
Aircraft Regional-Scale Flux Measurements over Complex Landscapes of Mangroves, Desert, and Marine Ecosystems of Magdalena Bay, Mexico
Natural ecosystems are rarely structurally simple or functionally homogeneous. This is true for the complex coastal region of Magdalena Bay, Baja California Sur, Mexico, where the spatial variability in ecosystem fluxes from the Pacific coastal ocean, eutrophic lagoon, mangroves, and desert were studied. The Sky Arrow 650TCN environmental research aircraft proved to be an effective tool in characterizing landâatmosphere fluxes of energy, CO2, and water vapor across a heterogeneous landscape at the scale of 1 km. The aircraft was capable of discriminating fluxes from all ecosystem types, as well as between nearshore and coastal areas a few kilometers distant. Aircraft-derived average midday CO2 fluxes from the desert showed a slight uptake of â1.32 ÎŒmol CO2 mâ2 sâ1, the coastal ocean also showed an uptake of â3.48 ÎŒmol CO2 mâ2 sâ1, and the lagoon mangroves showed the highest uptake of â8.11 ÎŒmol CO2 mâ2 sâ1. Additional simultaneous measurements of the normalized difference vegetation index (NDVI) allowed simple linear modeling of CO2 flux as a function of NDVI for the mangroves of the Magdalena Bay region. Aircraft approaches can, therefore, be instrumental in determining regional CO2 fluxes and can be pivotal in calculating and verifying ecosystem carbon sequestration regionally when coupled with satellite-derived products and ecosystem models
COSORE: A community database for continuous soil respiration and other soilâatmosphere greenhouse gas flux data
Globally, soils store two to three times as much carbon as currently resides in the atmosphere, and it is critical to understand how soil greenhouse gas (GHG) emissions and uptake will respond to ongoing climate change. In particular, the soilâtoâatmosphere CO2 flux, commonly though imprecisely termed soil respiration (RS), is one of the largest carbon fluxes in the Earth system. An increasing number of highâfrequency RS measurements (typically, from an automated system with hourly sampling) have been made over the last two decades; an increasing number of methane measurements are being made with such systems as well. Such high frequency data are an invaluable resource for understanding GHG fluxes, but lack a central database or repository. Here we describe the lightweight, openâsource COSORE (COntinuous SOil REspiration) database and software, that focuses on automated, continuous and longâterm GHG flux datasets, and is intended to serve as a community resource for earth sciences, climate change syntheses and model evaluation. Contributed datasets are mapped to a single, consistent standard, with metadata on contributors, geographic location, measurement conditions and ancillary data. The design emphasizes the importance of reproducibility, scientific transparency and open access to data. While being oriented towards continuously measured RS, the database design accommodates other soilâatmosphere measurements (e.g. ecosystem respiration, chamberâmeasured net ecosystem exchange, methane fluxes) as well as experimental treatments (heterotrophic only, etc.). We give brief examples of the types of analyses possible using this new community resource and describe its accompanying R software package
The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data
The FLUXNET2015 dataset provides ecosystem-scale data on CO2, water, and energy exchange between the biosphere and the atmosphere, and other meteorological and biological measurements, from 212 sites around the globe (over 1500 site-years, up to and including year 2014). These sites, independently managed and operated, voluntarily contributed their data to create global datasets. Data were quality controlled and processed using uniform methods, to improve consistency and intercomparability across sites. The dataset is already being used in a number of applications, including ecophysiology studies, remote sensing studies, and development of ecosystem and Earth system models. FLUXNET2015 includes derived-data products, such as gap-filled time series, ecosystem respiration and photosynthetic uptake estimates, estimation of uncertainties, and metadata about the measurements, presented for the first time in this paper. In addition, 206 of these sites are for the first time distributed under a Creative Commons (CC-BY 4.0) license. This paper details this enhanced dataset and the processing methods, now made available as open-source codes, making the dataset more accessible, transparent, and reproducible.Peer reviewe
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The use of made-up users
While the existence of fictitious users of financial statements has been confirmed in previous research, our study investigates how this powerful yet âmade-upâ construct is deployed within the discourses of the main stakeholders as they shape regulatory debates in the international accounting standard-setting arena, including ârealâ users themselves. Our study draws on Bourdieuâs theorization of dominant discourse as a form of power, and extends it with the phraseological theory of meaning, specifically the linguistic concept of collocation, which focuses on the habitual choices of words in discourse. Using this framework, we conduct a comparative analysis of the recurrent language choices around the term âuserâ in comment letters submitted on the selected IASBâs regulatory proposals. We provide empirical evidence for the existence of commonalties and subtle differences in the ways in which made-up users are discursively operationalized by the four key accounting constituent groups, the accounting profession, prepares, regulators and ârealâ users of financial statements. At the theoretical and methodological level, our study showcases the explanatory power of the concept of collocation to identify and interrogate implicit patterns of dominant discourse as set forth by Bourdieu. We also show that the close investigation of how the dominant discourse of the made-up users works generates a series of new why questions regarding the ârealâ usersâ role in accounting standard setting
AmeriFlux FLUXNET-1F US-Tw5 East Pond Wetland
This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Tw5 East Pond Wetland. This is the FLUXNET version of the carbon flux data for the site US-Tw5 East Pond Wetland produced by applying the standard ONEFlux (1F) software. Site Description - The Twitchell Wetland site is a 6.5 acre restored wetland on Twitchell Island, that is managed by the California Department of Water Resources (DWR) and the U.S. Geological Survey (USGS). In the fall of 1997, the site was permanently flooded to a depth of approximately 55 cm. The wetland remained fairly unvegetated in patches increasing in size towards the east. The site underwent a major disturbance in 2013 when the vegetation was removed to seed a nearby restored wetland. A flux tower equipped to analyze energy, H2O, CO2, and CH4 fluxes was installed on April 17, 2018
Glypican-3 mediated inhibition of CD26 by TFPI: a novel mechanism in hematopoietic stem cell homing and maintenance
Directional migration determines hematopoietic stem/progenitor cell (HSPC) homing, which depends upon the interaction between the chemokine CXCL12 and its receptor CXCR4. CD26 is a widely expressed membrane-bound ectopeptidase that cleaves CXCL12 thereby depleting its chemokine activity. We identified tissue-factor pathway inhibitor (TFPI) as a biological inhibitor of CD26 in murine and human HSPCs. We observed low-level TFPI expression in endothelial cells in the bone marrow (BM), which did not increase following radiation injury. Treatment of HSPCs with TFPI in vitro led to enhanced HSPC migration toward CXCL12, as well as homing and engraftment in the BM upon transplantation. We found that Glypican-3 (GPC3), a heparan sulfate proteoglycan expressed on murine as well as human HSPCs, mediated this effect. TFPI did not affect CD26 activity, migration, or homing of GPC3(-/-) HSPCs, while it affected GPC1(-/-) HSPCs similar to wild-type HSPCs. Moreover, proliferation of GPC3(-/-) but not GPC1(-/-) BM HSPCs was significantly increased, which was associated with a decrease in the primitive HSC pool in BM and an increase in proportion of the circulating HSPCs in the peripheral blood. Hence, we present a novel role for TFPI and GPC3 in regulating HSC homing as well as retention in the BM.status: publishe
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