NGC 7419: A young open cluster with a number of very young intermediate mass pre-MS stars

We present a photometric and spectroscopic study of the young open cluster NGC 7419, which is know to host a large number of classical Be stars for reasons not well understood. Based on CCD photometric observations of 327 stars in UBV passbands, we estimated the cluster parameters as, reddening E(B-V) = 1.65 +/- 0.15 mag and distance = 2900 +/- 400 pc. The turn off age of the cluster was estimated as 25 +/- 5 Myr using isochrone fits. UBV data of the stars were combined with JHK data from 2MASS and were used to create the near infrared (NIR) (J-H) vs (H-K) colour-colour diagram. A large fraction of stars (42%) was found to have NIR excess and their location in the diagram was used to identify them as intermediate mass pre-MS stars. The isochrone fits to pre-MS stars in the optical colour-magnitude diagram showed that the turn-on age of the cluster is 0.3 - 3 Myr. This indicates that there has been a recent episode of star formation in the vicinity of the cluster. Slit-less spectra were used to identify 27 stars which showed H-alpha in emission in the field of the cluster, of which 6 are new identifications. All these stars were found to show NIR excess and located closer to the region populated by Herbig Ae/Be stars in the (J-H) vs (H-K) diagram. Slit spectra of 25 stars were obtained in the region 3700A - 9000A. The spectral features were found to be very similar to those of Herbig Be stars. Those stars were found to be more reddened than the main sequence stars by 0.4 mag on an average. Thus the emission line stars found in this cluster are more similar to the Herbig Be type stars where the circumstellar material is the remnant of the accretion disk.Comment: 20 pages, 11 figures, Accepted for publishing in MNRAS on April 19, 200

Photometric study of distant open clusters in the second quadrant: NGC 7245, King 9, King 13 and IC 166

We present a UBV CCD photometric study of four open clusters, NGC 7245, King 9, IC 166 and King 13, located between l= 90° and 135°. All are embedded in a rich Galactic field. NGC 7245 and King 9 are close together in the sky and have similar reddenings. The distances and ages are: NGC 7245, 3.8 ± 0.35 kpc and 400 Myr; King 9 (the most distant cluster in this quadrant), 7.9 ± 1.1 kpc and 3.0 Gyr. King 13 is 3.1 ± 0.3 kpc distant and 300 Myr old. King 9 and IC 166 (4.8 ± 0.5 kpc distant and 1 Gyr old) may be metal-poor clusters (Z = 0.008), as estimated from isochrone fitting. The average value of the distance of young clusters from the Galactic plane in the above longitude range and beyond 2 kpc (−47 ± 16 pc, for 64 clusters) indicates that the young disc bends towards the southern latitudes

Analytical heat conduction model of annular composite fins

A two-dimensional analytical heat conduction model of an annular composite fin has been carried out. The composite fins composed of a porous polyethylene core, a square aluminum insert, and metallic zinc coating layers, was fabricated using wire-arc spraying technology. Analytical solutions of temperature distribution, energy dissipation and fin efficiency through the fins at natural convection condition have been proposed

Mannerisms of Millennials: Why are they different?

Differences in attitudes and mannerisms of the millennials and the older generation could result in intergenerational conflicts. This study aims to determine the definitions of good mannerisms as perceived by the two different generations and to identify the factors that contribute to the mannerisms and characteristics of the millennials. Using a non-experimental design, a questionnaire was distributed to 400 respondents. The responses by the millennials differed in some aspects from the older generation. Nevertheless, both groups agreed that the lack of a good upbringing and social media influences were the primary reasons for the lack of good mannerisms among millennials. Keywords: Millennials, Mannerism, Values, Characteristics eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open-access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under the responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians), and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia. DOI: https://doi.org/10.21834/ebpj.v7i21.369

Be phenomenon in open clusters: Results from a survey of emission-line stars in young open clusters

Emission-line stars in young open clusters are identified to study their properties, as a function of age, spectral type and their evolutionary state. 207 open star clusters were observed using slitless spectroscopy method and 157 emission stars were identified in 42 clusters. We have found 54 new emission-line stars in 24 open clusters, out of which 19 clusters are found to house emission stars for the first time. About 20% clusters harbour emission stars. The fraction of clusters housing emission stars is maximum in both the 0--10 and 20--30 Myr age bin ($\sim$ 40% each) and in the other age bins, this fraction ranges between 10% -- 25%, upto 80 Myr. We have used optical colour magnitude diagram (CMD) along with Near-IR Colour-Colour diagram (NIR CCDm) to classify the emission stars into Classical Be (CBe) stars and Herbig Be (HBe) stars. Most of the emission stars in our survey belong to CBe class ($\sim$ 92%) while a few are HBe stars ($\sim$ 6%) and HAe stars ($\sim$1%). The CBe stars are located all along the MS in the optical CMDs of clusters of all ages, which indicates that the Be phenomenon is unlikely due to core contraction near the turn-off. Most of the clusters which contain emission stars are found in Cygnus, Perseus & Monoceros region of the Galaxy, which are locations of active star formation. The distribution of CBe stars as a function of spectral type shows peaks at B1-B2 and B6-B7. Our results indicate there could be two mechanisms responsible for the CBe phenomenon. Some are born CBe stars (fast rotators), as indicated by their presence in clusters younger than 10 Myr. Some stars evolve to CBe stars, as indicates by the enhancement in the fraction of clusters with CBe stars in the 20-30 Myr age bin.Comment: 54 pages, 18 figures, Submitted to MNRA

TGF-β Inducible Early Gene 1 Regulates Osteoclast Differentiation and Survival by Mediating the NFATc1, AKT, and MEK/ERK Signaling Pathways

TGF-β Inducible Early Gene-1 (TIEG1) is a Krüppel-like transcription factor (KLF10) that was originally cloned from human osteoblasts as an early response gene to TGF-β treatment. As reported previously, TIEG1−/− mice have decreased cortical bone thickness and vertebral bone volume and have increased spacing between the trabeculae in the femoral head relative to wildtype controls. Here, we have investigated the role of TIEG1 in osteoclasts to further determine their potential role in mediating this phenotype. We have found that TIEG1−/− osteoclast precursors differentiated more slowly compared to wildtype precursors in vitro and high RANKL doses are able to overcome this defect. We also discovered that TIEG1−/− precursors exhibit defective RANKL-induced phosphorylation and accumulation of NFATc1 and the NFATc1 target gene DC-STAMP. Higher RANKL concentrations reversed defective NFATc1 signaling and restored differentiation. After differentiation, wildtype osteoclasts underwent apoptosis more quickly than TIEG1−/− osteoclasts. We observed increased AKT and MEK/ERK signaling pathway activation in TIEG1−/− osteoclasts, consistent with the roles of these kinases in promoting osteoclast survival. Adenoviral delivery of TIEG1 (AdTIEG1) to TIEG1−/− cells reversed the RANKL-induced NFATc1 signaling defect in TIEG1−/− precursors and eliminated the differentiation and apoptosis defects. Suppression of TIEG1 with siRNA in wildtype cells reduced differentiation and NFATc1 activation. Together, these data provide evidence that TIEG1 controls osteoclast differentiation by reducing NFATc1 pathway activation and reduces osteoclast survival by suppressing AKT and MEK/ERK signaling

The RNA-Binding Protein Musashi1 Affects Medulloblastoma Growth via a Network of Cancer- Related Genes and Is an Indicator of Poor Prognosis

Musashi1 (Msi1) is a highly conserved RNA-binding protein that is required during the development of the nervous system. Msi1 has been characterized as a stem cell marker, controlling the balance between self-renewal and differentiation, and has also been implicated in tumorigenesis, being highly expressed in multiple tumor types. We analyzed Msi1 expression in a large cohort of medulloblastoma samples and found that Msi1 is highly expressed in tumor tissue compared with normal cerebellum. Notably, high Msi1 expression levels proved to be a sign of poor prognosis. Msi1 expression was determined to be particularly high in molecular subgroups 3 and 4 of medulloblastoma. We determined that Msi1 is required for tumorigenesis because inhibition of Msi1 expression by small-interfering RNAs reduced the growth of Daoy medulloblastoma cells in xenografts. To characterize the participation of Msi1 in medulloblastoma, we conducted different high-throughput analyses. Ribonucleoprotein immunoprecipitation followed by microarray analysis (RIP-chip) was used to identify mRNA species preferentially associated with Msi1 protein in Daoy cells. We also used cluster analysis to identify genes with similar or opposite expression patterns to Msi1 in our medulloblastoma cohort. A network study identified RAC1, CTGF, SDCBP, SRC, PRL, and SHC1 as major nodes of an Msi1-associated network. Our results suggest that Msi1 functions as a regulator of multiple processes in medulloblastoma formation and could become an important therapeutic target

Aggregated a-synuclein and complex I deficiency: exploration of their relationship in differentiated neurons

α-Synuclein becomes misfolded and aggregated upon damage by various factors, for example, by reactive oxygen species. These aggregated forms have been proposed to have differential toxicities and their interaction with mitochondria may cause dysfunction within this organelle that contributes to the pathogenesis of Parkinson’s disease (PD). In particular, the association of α-synuclein with mitochondria occurs through interaction with mitochondrial complex I and importantly defects of this protein have been linked to the pathogenesis of PD. Therefore, we investigated the relationship between aggregated α-synuclein and mitochondrial dysfunction, and the consequences of this interaction on cell survival. To do this, we studied the effects of α-synuclein on cybrid cell lines harbouring mutations in either mitochondrial complex I or IV. We found that aggregated α-synuclein inhibited mitochondrial complex I in control and complex IV-deficient cells. However, when aggregated α-synuclein was applied to complex I-deficient cells, there was no additional inhibition of mitochondrial function or increase in cell death. This would suggest that as complex I-deficient cells have already adapted to their mitochondrial defect, the subsequent toxic effects of α-synuclein are reduced

Radio, millimeter and optical monitoring of GRB030329 afterglow: Constraining the double jet model

We present radio, millimeter and optical observations of the afterglow of GRB030329. UBVR_{C}I_{C} photometry is presented for a period of 3 hours to 34 days after the burst. Radio monitoring at 1280 MHz has been carried out using the GMRT for more than a year. Simultaneous millimeter observations at 90 GHz and 230 GHz have been obtained from the Swedish-ESO Submillimeter Telescope (SEST) and the IRAM-PdB interferometer over more than a month following the burst. We use these data to constrain the double jet model proposed by Berger et al. (2003) for this afterglow. We also examine whether instead of the two jets being simultaneously present, the wider jet could result from the initially narrow jet, due to a fresh supply of energy from the central engine after the ``jet break''.Comment: 31 pages, 5 figures, Accepted for publication in A&A main journal. Table 2 will be available only through the electronic version of the journa

A multi-targeted approach to suppress tumor-promoting inflammation

Cancers harbor significant genetic heterogeneity and patterns of relapse following many therapies are due to evolved resistance to treatment. While efforts have been made to combine targeted therapies, significant levels of toxicity have stymied efforts to effectively treat cancer with multi-drug combinations using currently approved therapeutics. We discuss the relationship between tumor-promoting inflammation and cancer as part of a larger effort to develop a broad-spectrum therapeutic approach aimed at a wide range of targets to address this heterogeneity. Specifically, macrophage migration inhibitory factor, cyclooxygenase-2, transcription factor nuclear factor-κB, tumor necrosis factor alpha, inducible nitric oxide synthase, protein kinase B, and CXC chemokines are reviewed as important antiinflammatory targets while curcumin, resveratrol, epigallocatechin gallate, genistein, lycopene, and anthocyanins are reviewed as low-cost, low toxicity means by which these targets might all be reached simultaneously. Future translational work will need to assess the resulting synergies of rationally designed antiinflammatory mixtures (employing low-toxicity constituents), and then combine this with similar approaches targeting the most important pathways across the range of cancer hallmark phenotypes