RNA Oligonucleotide Fingerprint Analysis of Dengue Serotype 3 and 4 Viruses Isolated in the Southeast Asia

東南アジアにて分離されたデングウイルス3型と4型を,RNase-T1オリゴヌクレオチドフィンガープリント法により比較検討した.3型と4型の株間では,スポットのパターンは明らかに異なっていた.分離年代が10年ほど差があるフィリピンで分離された3型株は互いによく似ており,インドネシアで分離された3型株も互いによく似ていたが,フィリピン,インドネシア及びタイでそれぞれ分離された株では明らかに地域差がみられた.これらのことは,デングウイルス3型遺伝子の変異と撰択とが,これら地理的に充分離れた地域では,それぞれ独立して進行したことを示唆している.実験室内感染患者より分離後,乳のみマウス脳10代継代された4型ウイルスは患者に感染前の株とかなり類似していたことより,デングウイルス4型遺伝子はこの程度の継代ではあまり変化しないものと考えられる.Oligonucleotide fingerprints of the 42S genome RNA of dengue (DEN) serotype 3 and 4 virus isolated in the Southeast Asia were examined, DEN type 3 (DEN-3) virus strains were apparently different from DEN type 4 (DEN-4) virus strains in their fingerprint patterns. Two DEN-3 isolates in the Philippines were rather similar each other, and also three DEN-3 isolates in Indonesia were rather similar, whereas the isolates in the Philippines, Indonesia, and Thailand were significantly different from each other. The result suggests that mutations and selections of DEN-3 viral genome would have progressed independently in these geographically distant areas. The DEN-4 virus isolated from the serum of a patient with laboratory infection and passaged 10 times in suckling mouse brain (SMB) was considerably similar to its parent strain, suggesting that the viral genome would not have changed so much by these passages

Ticks Associated with Macquarie Island Penguins Carry Arboviruses from Four Genera

Macquarie Island, a small subantarctic island, is home to rockhopper, royal and king penguins, which are often infested with the globally distributed seabird tick, Ixodes uriae. A flavivirus, an orbivirus, a phlebovirus, and a nairovirus were isolated from these ticks and partial sequences obtained. The flavivirus was nearly identical to Gadgets Gully virus, isolated some 30 year previously, illustrating the remarkable genetic stability of this virus. The nearest relative to the orbivirus (for which we propose the name Sandy Bay virus) was the Scottish Broadhaven virus, and provided only the second available sequences from the Great Island orbivirus serogroup. The phlebovirus (for which we propose the name Catch-me-cave virus) and the previously isolated Precarious Point virus were distinct but related, with both showing homology with the Finnish Uukuniemi virus. These penguin viruses provided the second and third available sequences for the Uukuniemi group of phleboviruses. The nairovirus (for which we propose the name Finch Creek virus) was shown to be related to the North American Tillamook virus, the Asian Hazara virus and Nairobi sheep disease virus. Macquarie Island penguins thus harbour arboviruses from at least four of the seven arbovirus-containing genera, with related viruses often found in the northern hemisphere

Global age-sex-specific fertility, mortality, healthy life expectancy (HALE), and population estimates in 204 countries and territories, 1950-2019 : a comprehensive demographic analysis for the Global Burden of Disease Study 2019

Background Accurate and up-to-date assessment of demographic metrics is crucial for understanding a wide range of social, economic, and public health issues that affect populations worldwide. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 produced updated and comprehensive demographic assessments of the key indicators of fertility, mortality, migration, and population for 204 countries and territories and selected subnational locations from 1950 to 2019. Methods 8078 country-years of vital registration and sample registration data, 938 surveys, 349 censuses, and 238 other sources were identified and used to estimate age-specific fertility. Spatiotemporal Gaussian process regression (ST-GPR) was used to generate age-specific fertility rates for 5-year age groups between ages 15 and 49 years. With extensions to age groups 10-14 and 50-54 years, the total fertility rate (TFR) was then aggregated using the estimated age-specific fertility between ages 10 and 54 years. 7417 sources were used for under-5 mortality estimation and 7355 for adult mortality. ST-GPR was used to synthesise data sources after correction for known biases. Adult mortality was measured as the probability of death between ages 15 and 60 years based on vital registration, sample registration, and sibling histories, and was also estimated using ST-GPR. HIV-free life tables were then estimated using estimates of under-5 and adult mortality rates using a relational model life table system created for GBD, which closely tracks observed age-specific mortality rates from complete vital registration when available. Independent estimates of HIV-specific mortality generated by an epidemiological analysis of HIV prevalence surveys and antenatal clinic serosurveillance and other sources were incorporated into the estimates in countries with large epidemics. Annual and single-year age estimates of net migration and population for each country and territory were generated using a Bayesian hierarchical cohort component model that analysed estimated age-specific fertility and mortality rates along with 1250 censuses and 747 population registry years. We classified location-years into seven categories on the basis of the natural rate of increase in population (calculated by subtracting the crude death rate from the crude birth rate) and the net migration rate. We computed healthy life expectancy (HALE) using years lived with disability (YLDs) per capita, life tables, and standard demographic methods. Uncertainty was propagated throughout the demographic estimation process, including fertility, mortality, and population, with 1000 draw-level estimates produced for each metric. Findings The global TFR decreased from 2.72 (95% uncertainty interval [UI] 2.66-2.79) in 2000 to 2.31 (2.17-2.46) in 2019. Global annual livebirths increased from 134.5 million (131.5-137.8) in 2000 to a peak of 139.6 million (133.0-146.9) in 2016. Global livebirths then declined to 135.3 million (127.2-144.1) in 2019. Of the 204 countries and territories included in this study, in 2019, 102 had a TFR lower than 2.1, which is considered a good approximation of replacement-level fertility. All countries in sub-Saharan Africa had TFRs above replacement level in 2019 and accounted for 27.1% (95% UI 26.4-27.8) of global livebirths. Global life expectancy at birth increased from 67.2 years (95% UI 66.8-67.6) in 2000 to 73.5 years (72.8-74.3) in 2019. The total number of deaths increased from 50.7 million (49.5-51.9) in 2000 to 56.5 million (53.7-59.2) in 2019. Under-5 deaths declined from 9.6 million (9.1-10.3) in 2000 to 5.0 million (4.3-6.0) in 2019. Global population increased by 25.7%, from 6.2 billion (6.0-6.3) in 2000 to 7.7 billion (7.5-8.0) in 2019. In 2019, 34 countries had negative natural rates of increase; in 17 of these, the population declined because immigration was not sufficient to counteract the negative rate of decline. Globally, HALE increased from 58.6 years (56.1-60.8) in 2000 to 63.5 years (60.8-66.1) in 2019. HALE increased in 202 of 204 countries and territories between 2000 and 2019. Interpretation Over the past 20 years, fertility rates have been dropping steadily and life expectancy has been increasing, with few exceptions. Much of this change follows historical patterns linking social and economic determinants, such as those captured by the GBD Socio-demographic Index, with demographic outcomes. More recently, several countries have experienced a combination of low fertility and stagnating improvement in mortality rates, pushing more populations into the late stages of the demographic transition. Tracking demographic change and the emergence of new patterns will be essential for global health monitoring. Copyright (C) 2020 The Author(s). Published by Elsevier Ltd.Peer reviewe

Measurement of t(t)over-bar normalised multi-differential cross sections in pp collisions at root s=13 TeV, and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Peer reviewe

Measurement of the top quark mass in the all- jets final state at root s=13 TeV and combination with the lepton plus jets channel

A top quark mass measurement is performed using 35.9 fb - 1 of LHC proton- proton collision data collected with the CMS detector at v s = 13 TeV. The measurement uses the tt all- jets final state. A kinematic fit is performed to reconstruct the decay of the tt system and suppress themultijet background. Using the ideogram method, the top quark mass ( mt) is determined, simultaneously constraining an additional jet energy scale factor ( JSF). The resulting value of mt = 172.34 +/- 0.20 ( stat+ JSF) +/- 0.70 ( syst) GeV is in good agreement with previous measurements. In addition, a combined measurement that uses the tt lepton+ jets and all- jets final states is presented, using the same mass extraction method, and provides an mt measurement of 172.26 +/- 0.07 ( stat+ JSF) +/- 0.61 ( syst) GeV. This is the first combined mt extraction from the lepton+ jets and all- jets channels through a single likelihood function.Peer reviewe

Measurement of tt̄ normalised multi-differential cross sections in pp collisions at √s = 13 TeV, and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Normalised multi-differential cross sections for top quark pair (tt̄) production are measured in proton-proton collisions at a centre-of-mass energy of 13 TeV using events containing two oppositely charged leptons. The analysed data were recorded with the CMS detector in 2016 and correspond to an integrated luminosity of 35.9fb⁻¹. The double-differential tt̄ cross section is measured as a function of the kinematic properties of the top quark and of the tt̄ system at parton level in the full phase space. A triple-differential measurement is performed as a function of the invariant mass and rapidity of the tt̄ system and the multiplicity of additional jets at particle level. The data are compared to predictions of Monte Carlo event generators that complement next-to-leading-order (NLO) quantum chromodynamics (QCD) calculations with parton showers. Together with a fixed-order NLO QCD calculation, the triple-differential measurement is used to extract values of the strong coupling strength αS and the top quark pole mass (m_(pole_t) using several sets of parton distribution functions (PDFs). The measurement of m_(pole)_t exploits the sensitivity of the tt̄ invariant mass distribution to m_(pole)_t near the production threshold. Furthermore, a simultaneous fit of the PDFs, α_S, and m_(pole)_t is performed at NLO, demonstrating that the new data have significant impact on the gluon PDF, and at the same time allow an accurate determination of α_S and m_(pole)_t. The values α_S(m_Z) = 0.1135+0.0021−0.0017 and m_(pole)_t = 170.5±0.8GeV are extracted, which account for experimental and theoretical uncertainties, the latter being estimated from NLO scale variations. Possible effects from Coulomb and soft-gluon resummation near the tt̄ production threshold are neglected in these parameter extractions. A rough estimate of these effects indicates an expected correction of m_(pole)_t of the order of +1 GeV, which can be regarded as additional theoretical uncertainty in the current m_(pole)_t extraction

An embedding technique to determine ττ backgrounds in proton-proton collision data

An embedding technique is presented to estimate standard model tau tau backgrounds from data with minimal simulation input. In the data, the muons are removed from reconstructed mu mu events and replaced with simulated tau leptons with the same kinematic properties. In this way, a set of hybrid events is obtained that does not rely on simulation except for the decay of the tau leptons. The challenges in describing the underlying event or the production of associated jets in the simulation are avoided. The technique described in this paper was developed for CMS. Its validation and the inherent uncertainties are also discussed. The demonstration of the performance of the technique is based on a sample of proton-proton collisions collected by CMS in 2017 at root s = 13 TeV corresponding to an integrated luminosity of 41.5 fb(-1).Peer reviewe