GANGRENA DIGITAL EM EXTREMIDADE SUPERIOR SECUNDÁRIA À INFUSÃO INTRAVENOSA LOCAL DE VANCOMICINA: RELATO DE CASO

http://dx.doi.org/10.5902/223658346378RESUMO Em decorrência de sua crescente recomendação para tratamento de infecções hospitalares por Staphilococcus sp. com resistência à meticilina e oxacilina, a utilização do antibiótico glicopeptídeo Vancomicina aumentou muito nos últimos 20 anos. Apesar desde fato, o número de pacientes que eventualmente apresenta efeitos adversos graves à sua administração é muito pequeno, sendo mais frequentes reações simples como dor, tromboflebite no local de administração, febre e calafrios. Entre as complicações graves relacionadas ao seu uso estão a anafilaxia, a necrólise epidérmica tóxica, o eritema multiforme, a ototoxicidade e a ‘síndrome do homem vermelho’. Neste artigo descrevemos um caso muito raro e excepcional de gangrena isquêmica de todos os quirodáctilos da mão direita após infusão intravenosa local de vancomicina. Descritores: vancomicina, efeitos adversos, toxicidade, gangrena

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe

Passion fruit seed meal at growing and finishing pig (30-90 kg) feeding

The passion fruit seed is a byproduct of the extraction of the passion fruit pulp and can be used for feeding to pigs. Two experiments were conducted (digestibility and performance) to evaluate the use of the passion fruit seed meal in feed for growing and finishing pigs. A digestibility trial was conducted with growing and finishing pigs, in which the passion fruit seed meal (PSM) replaced the reference diet (0% PSM) in levels of 4, 8, 12 and 16%. Thirty barrows were used with average weight of 36.85±4.19. The PSF showed DE and ME of 3.244 and 3.223 Mcal/kg, respectively. In the performance trial, were used 150 pigs, which 75 in the growing phase and 75 pigs in the finishing, with initial body weight of 30.63±1.49 and final of 60.38±4.75 kg and 60.40±1.50 and 90.02±4.84 kg, respectively. Five diets were used, with four inclusion levels of PSM (4, 8, 12 e 16%) and one control diet (0% of PSM). The PSM levels did not influence (P>0.05) the performance variables. In the growing pigs, serum levels of cholesterol increased linearly with (P0.05) by increasing levels of PSM in the diets. We conclude that the PSM can be used up to 16% in growing and finishing pigs diets

Assessing non-synthetic crosslinkers in biomaterial inks based on polymers of marine origin to increase the shape fidelity in 3D extrusion printing

16 pages, 9 figures.-- Open accessIn the past decade, there has been significant progress in 3D printing research for tissue engineering (TE) using biomaterial inks made from natural and synthetic compounds. These constructs can aid in the regeneration process after tissue loss or injury, but achieving high shape fidelity is a challenge as it affects the construct's physical and biological performance with cells. In parallel with the growth of 3D bioprinting approaches, some marine-origin polymers have been studied due to their biocompatibility, biodegradability, low immunogenicity, and similarities to human extracellular matrix components, making them an excellent alternative to land mammal-origin polymers with reduced disease transmission risk and ethical concerns. In this research, collagen from shark skin, chitosan from squid pens, and fucoidan from brown algae were effectively blended for the manufacturing of an adequate biomaterial ink to achieve a printable, reproducible material with a high shape fidelity and reticulated using four different approaches (phosphate-buffered saline, cell culture medium, 6% CaCl2, and 5 mM Genipin). Materials characterization was composed by filament collapse, fusion behavior, swelling behavior, and rheological and compressive tests, which demonstrated favorable shape fidelity resulting in a stable structure without deformations, and interesting shear recovery properties around the 80% mark. Additionally, live/dead assays were conducted in order to assess the cell viability of an immortalized human mesenchymal stem cell line, seeded directly on the 3D printed constructs, which showed over 90% viable cells. Overall, the Roswell Park Memorial Institute cell culture medium promoted the adequate crosslinking of this biopolymer blend to serve the TE approach, taking advantage of its capacity to hamper pH decrease coming from the acidic biomaterial ink. While the crosslinking occurs, the pH can be easily monitored by the presence of the indicator phenol red in the cell culture medium, which reduces costs and timeThe authors would like to acknowledge to Portuguese Foundation of Science and Technology (FCT) for PhD fellowship (D N Carvalho) under the scope of the doctoral program Tissue Engineering, Regenerative Medicine and Stem Cells, Ref. PD/BD/143044/2018, and The German Research Foundation (S Dani). The Article Processing Charges (APC) for making the article Open Access were funded by the joint publication funds of the TU Dresden, including Carl Gustav Carus Faculty of Medicine, and the SLUB Dresden as well as the Open Access Publication Funding of the German Reearch Foundation (DFG)Peer reviewe