705 research outputs found
Identification of novel candidate target genes, including EPHB3, MASP1 and SST at 3q26.2âq29 in squamous cell carcinoma of the lung
- Author
- AL Tai
- B Angulo
- CC Yen
- D Pinkel
- DM Parkin
- DS Salomon
- F Jiang
- FR Hirsch
- Garnis1 Cathie
- GY Lee
- H Willenbrock
- I Lukashova-v Zangen
- J Pardo
- J Qian
- J Tong
- JI Kim
- Ji Un Kang
- Jin Man Kim
- JJ Park
- Jong Woo Park
- JU Kang
- JU Kang
- JU Kang
- JU Kang
- K Heselmeyer
- K Heselmeyer
- K Lundgren
- Kye Chul Kwon
- L Roque
- M Chujo
- M Höglund
- M Kuraya
- M Nanjundan
- M Varella-Garcia
- MM Weiss
- P Loukopoulos
- PP Massion
- S Okada
- S Yokoi
- SM Sy
- SM Sy
- Sun Hoe Koo
- T Ubagai
- T Yakut
- W Liu
- WS Yan
- WW Lockwood
- Y-W Choi
- YW Choi
- YW Choi
- Publication venue
- BioMed Central
- Publication date
- 01/01/2009
- Field of study
<p>Abstract</p> <p>Background</p> <p>The underlying genetic alterations for squamous cell carcinoma (SCC) and adenocarcinoma (AC) carcinogenesis are largely unknown.</p> <p>Methods</p> <p>High-resolution array- CGH was performed to identify the differences in the patterns of genomic imbalances between SCC and AC of non-small cell lung cancer (NSCLC).</p> <p>Results</p> <p>On a genome-wide profile, SCCs showed higher frequency of gains than ACs (<it>p </it>= 0.067). More specifically, statistically significant differences were observed across the histologic subtypes for gains at 2q14.2, 3q26.2âq29, 12p13.2âp13.33, and 19p13.3, as well as losses at 3p26.2âp26.3, 16p13.11, and 17p11.2 in SCC, and gains at 7q22.1 and losses at 15q22.2âq25.2 occurred in AC (<it>P </it>< 0.05). The most striking difference between SCC and AC was gains at the 3q26.2âq29, occurring in 86% (19/22) of SCCs, but in only 21% (3/14) of ACs. Many significant genes at the 3q26.2âq29 regions previously linked to a specific histology, such as EVI1,<it>MDS1, PIK3CA </it>and <it>TP73L</it>, were observed in SCC (<it>P </it>< 0.05). In addition, we identified the following possible target genes (> 30% of patients) at 3q26.2âq29: <it>LOC389174 </it>(3q26.2),<it>KCNMB3 </it>(3q26.32),<it>EPHB3 </it>(3q27.1), <it>MASP1 </it>and <it>SST </it>(3q27.3), <it>LPP </it>and <it>FGF12 </it>(3q28), and <it>OPA1</it>,<it>KIAA022</it>,<it>LOC220729</it>, <it>LOC440996</it>,<it>LOC440997</it>, and <it>LOC440998 </it>(3q29), all of which were significantly targeted in SCC (<it>P </it>< 0.05). Among these same genes, high-level amplifications were detected for the gene, <it>EPHB3</it>, at 3q27.1, and <it>MASP1 </it>and <it>SST</it>, at 3q27.3 (18, 18, and 14%, respectively). Quantitative real time PCR demonstrated array CGH detected potential candidate genes that were over expressed in SCCs.</p> <p>Conclusion</p> <p>Using whole-genome array CGH, we have successfully identified significant differences and unique information of chromosomal signatures prevalent between the SCC and AC subtypes of NSCLC. The newly identified candidate target genes may prove to be highly attractive candidate molecular markers for the classification of NSCLC histologic subtypes, and could potentially contribute to the pathogenesis of the squamous cell carcinoma of the lung.</p
A placebo-controlled trial of Korean red ginseng extract for preventing Influenza-like illness in healthy adults
- Author
- AG Jansen
- BF Johnson
- BK Tan
- CB Hall
- Chang-Seop Lee
- DA Tyrrell
- Dae-Young Kwon
- DS Lim
- Eun-Kyung Choi
- Eun-Ok Park
- F Scaglione
- F Scaglione
- G Cilla
- GL Kirkpatrick
- GN Predy
- Hee-Joo Kang
- Hyang-Im Back
- Hye-Jeong Yang
- J Flamaing
- JE McElhaney
- JF Aguilera
- JM Gwaltney Jr
- JP Engel
- Ju-Hyung Lee
- JY Song
- KH Kim
- Ki-Chan Ha
- KL Nichol
- KS Shin
- Kyung-Min Choi
- M Camps
- M Kaye
- M Tomoda
- M Wang
- Mi-Ra Oh
- Min-Gul Kim
- Min-Jeong Kim
- RJ Singleton
- S Vohra
- Soo-Wan Chae
- SR Lowenstein
- Sun-Young Kim
- Publication venue
- BioMed Central
- Publication date
- 01/01/2012
- Field of study
<p>Abstracts</p> <p>Background</p> <p>Standardized Korean red ginseng extract has become the best-selling influenza-like illness (ILI) remedy in Korea, yet much controversy regarding the efficacy of the Korean red ginseng (KRG) in reducing ILI incidence remains. The aim of the study is to assess the efficacy of the KRG extract on the ILI incidence in healthy adults.</p> <p>Methods/Design</p> <p>We will conduct a randomized, double-blind, placebo-controlled study at the onset of the influenza seasons. A total of 100 subjects 30-70 years of age will be recruited from the general populations. The subjects will be instructed to take 9 capsules per day of either the KRG extract or a placebo for a period of 3 months. The primary outcome measure is to assess the frequency of ILI onset in participated subjects. Secondary variable measures will be included severity and duration of ILI symptoms. The ILI symptoms will be scored by subjects using a 4-point scale.</p> <p>Discussion</p> <p>This study is a randomized placebo controlled trial to evaluate the efficacy of the KRG extract compared to placebo and will be provided valuable new information about the clinical and physiological effects of the KRG extract on reduction of ILI incidence including flu and upper respiratory tract infections. The study has been pragmatically designed to ensure that the study findings can be implemented into clinical practice if KRG extract can be shown to be an effective reduction strategy in ILI incidence.</p> <p>Trial Registration</p> <p><a href="http://www.clinicaltrials.gov/ct2/show/NCT01478009">NCT01478009</a>.</p
Involvement of Cox-2 in the metastatic potential of chemotherapy-resistant breast cancer cells
- Author
- A Di Popolo
- A Jemal
- A Ristimaki
- A Takaoka
- AJ Dannenberg
- AM Simeone
- B Singh
- C Ceccarelli
- C Cherbonnel-Lasserre
- Chang Hoon Lee
- Changsun Choi
- CL Reimer
- CM Rudin
- CS Williams
- D Luo
- D Ratnasinghe
- DS Kim
- E Shtivelman
- EH Kim
- G Ferrandina
- G Li
- HJ Kim
- J Bange
- J Williams
- Ju-Hee Kang
- K Araki
- K Park
- K Xu
- KH Chun
- Ki-Hoon Song
- Kyung-Chae Jeong
- M Yao
- MC Zatelli
- ME St-Germain
- MW Chan
- NY Lukyanova
- P Vihinen
- PD Ryan
- Q Jiang
- R Wang
- RE Harris
- RS Kerbel
- S Narumiya
- Seung Hyun Oh
- SH Oh
- Sunshin Kim
- Y Kurihara
- Publication venue
- BioMed Central
- Publication date
- 01/01/2011
- Field of study
<p>Abstract</p> <p>Background</p> <p>A major problem with the use of current chemotherapy regimens for several cancers, including breast cancer, is development of intrinsic or acquired drug resistance, which results in disease recurrence and metastasis. However, the mechanisms underlying this drug resistance are unknown. To study the molecular mechanisms underlying the invasive and metastatic activities of drug-resistant cancer cells, we generated a doxorubicin-resistant MCF-7 breast cancer cell line (MCF-7/DOX).</p> <p>Methods</p> <p>We used MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, flow cytometry assays, DNA fragmentation assays, Western blot analysis, cell invasion assays, small interfering RNA (siRNA) transfection, reverse transcription-polymerase chain reaction, experimental lung metastasis models, and gelatin and fibrinogen/plasminogen zymography to study the molecular mechanism of metastatic activities in MCF-7/DOX cells.</p> <p>Results</p> <p>We found that MCF-7/DOX acquired invasive activities. In addition, Western blot analysis showed increased expression of epidermal growth factor receptor (EGFR) and Cox-2 in MCF-7/DOX cells. Inhibition of Cox-2, phosphoinositide 3-kinase (PI3K)/Akt, or mitogen-activated protein kinase (MAPK) pathways effectively inhibited the invasive activities of MCF-7/DOX cells. Gelatin and fibrinogen/plasminogen zymography analysis showed that the enzymatic activities of matrix metalloproteinase-2 (MMP-2), MMP-9, and urokinase-type plasminogen activator were markedly higher in MCF-7/DOX cells than in the MCF-7 cells. <it>In vitro </it>invasion assays and mouse models of lung metastasis demonstrated that MCF-7/DOX cells acquired invasive abilities. Using siRNAs and agonists specific for prostaglandin E (EP) receptors, we found that EP1 and EP3 played important roles in the invasiveness of MCF-7/DOX cells.</p> <p>Conclusions</p> <p>We found that the invasive activity of MCF-7/DOX cells is mediated by Cox-2, which is induced by the EGFR-activated PI3K/Akt and MAPK pathways. In addition, EP1 and EP3 are important in the Cox-2-induced invasion of MCF-7/DOX cells. Therefore, not only Cox-2 but also EP1 and EP3 could be important targets for chemosensitization and inhibition of metastasis in breast cancers that are resistant to chemotherapy.</p
Phosphorylation of Nicastrin by SGK1 Leads to Its Degradation through Lysosomal and Proteasomal Pathways
- Author
- A Herreman
- AM Bogusz
- B De Strooper
- B De Strooper
- B De Strooper
- C Brou
- C Chen
- C Goutte
- C Haass
- C Kaether
- C Shelly
- C Shen
- CJ Phiel
- D Gianni
- D Pearce
- D Pearce
- DR Dries
- DS Yang
- E Kamynina
- EC Lai
- EH Schroeter
- Eui-Ju Choi
- Eun-Jung Ann
- F Lang
- Florian Lang
- G He
- G Struhl
- G Yu
- GL Firestone
- H Cheng
- H Karlstrom
- H Sakoda
- H Steiner
- Hee-Sae Park
- Hyeong-Jin Baek
- Jean Kanellopoulos
- Ji-Ae Hong
- Ji-Hye Yoon
- Ji-Seon Ahn
- JS Mo
- Jung-Soon Mo
- K Shirotani
- K Tang
- LH Kuo
- M Niimura
- MF Arteaga
- MF Arteaga
- Mi-Yeon Kim
- MK Webster
- MS Wolfe
- MY Kim
- O Berezovska
- P Buse
- P Cohen
- P Kehoe
- P Wulff
- R Francis
- R Kopan
- R Pardossi-Piquard
- R Siman
- R Vassar
- RD Bagshaw
- S Prokop
- S Shah
- SF Lee
- SH Kim
- SH Pasternak
- SK Kim
- Su-Man Kim
- T Kobayashi
- T Maeda
- WJ Luo
- WT Kimberly
- Y Ye
- YW Zhang
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2012
- Field of study
The gamma-secretase complex is involved in the intramembranous proteolysis of a variety of substrates, including the amyloid precursor protein and the Notch receptor. Nicastrin (NCT) is an essential component of the gamma-secretase complex and functions as a receptor for gamma-secretase substrates. In this study, we determined that serum- and glucocorticoid-induced protein kinase 1 (SGK1) markedly reduced the protein stability of NCT. The SGK1 kinase activity was decisive for NCT degradation and endogenous SGK1 inhibited gamma-secretase activity. SGK1 downregulates NCT protein levels via proteasomal and lysosomal pathways. Furthermore, SGK1 directly bound to and phosphorylated NCT on Ser437, thereby promoting protein degradation. Collectively, our findings indicate that SGK1 is a gamma-secretase regulator presumably effective through phosphorylation and degradation of NCT
Bio-anthropological Studies on Human Skeletons from the 6th Century Tomb of Ancient Silla Kingdom in South Korea
- Author
- A Fujii
- A. Young Yoon
- AH Thompson
- Caroline M. Wilkinson
- Chang Seok Oh
- DH Shin
- Dong Hoon Shin
- DS Lim
- DY Kang
- DY Yang
- Eun Jin Woo
- GV Lebedinskaya
- HY Lee
- I Hershkovitz
- JA Yu
- Jeong A. Yoo
- Jin Og Ju
- Jong Ha Hong
- JY Shin
- K Choy
- K Choy
- K Pearson
- KC Choy
- KS Kho
- KS Koh
- L Fan
- M Fantini
- M Hofreiter
- M Yoneda
- Mark Spigelman
- MP Richards
- MS Nelson
- S Benazzi
- SB Hong
- SH Han
- SI Kvaal
- Soon Jo Choi
- Soong Doek Lee
- SY Kang
- T Hanihara
- T Nagaoka
- T Tsutaya
- TD White
- WJ Lee
- WJ Lee
- WJ Lee
- Won-Joon Lee
- Yi-Suk Kim
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/06/2016
- Field of study
In November and December 2013, unidentified human skeletal remains buried in a mokgwakmyo
(a traditional wooden coffin) were unearthed while conducting an archaeological investigation near Gyeongju, which was the capital of the Silla Kingdom (57 BCEâ 660 CE) of ancient Korea. The human skeletal remains were preserved in relatively intact condition.
In an attempt to obtain biological information on the skeleton, physical anthropological, mitochondrial
DNA, stable isotope and craniofacial analyses were carried out. The results indicated that the individual was a female from the Silla period, of 155 ± 5 cm height, who died in her late thirties. The maternal lineage belonged to the haplogroup F1b1a, typical for East Asia, and the diet had been more C3- (wheat, rice and potatoes) than C4-based (maize, millet and other tropical grains). Finally, the face of the individual was reconstructed utilizing the skull (restored from osseous fragments) and three-dimensional computerized modelling system. This study, applying multi-dimensional approaches within an overall bio-anthropological analysis, was the first attempt to collect holistic biological information on human skeletal remains dating to the Silla Kingdom period of ancient Korea
Search for new phenomena in final states with an energetic jet and large missing transverse momentum in pp collisions at â s = 8 TeV with the ATLAS detector
- Author
- Aad G
- Abbott B
- Abbott DC
- Abeling K
- Abhayasinghe DK
- Abidi SH
- AbouZeid OS
- Abraham NL
- Abramowicz H
- Abreu H
- Abud AA
- Abulaiti Y
- Acharya BS
- Achkar B
- Adachi S
- Adam L
- Adamczyk L
- Adamek L
- Adelman J
- Adersberger M
- Adiguzel A
- Adorni S
- Adye T
- Affolder AA
- Afik Y
- Agapopoulou C
- Agaras MN
- Aggarwal A
- Agheorghiesei C
- Aguilar-Saavedra JA
- Ahmadov F
- Ahmed WS
- Ai X
- Aielli G
- Akatsuka S
- Akesson TPA
- Akilli E
- Akimov A
- Al Khoury K
- Alberghi GL
- Albert J
- Alderweireldt S
- Aleksa M
- Aleksandrov IN
- Alexa C
- Alexopoulos T
- Alfonsi A
- Alfonsi F
- Alhroob M
- Ali B
- Aliev M
- Alimonti G
- Allaire C
- Allbrooke BMM
- Allen BW
- Allport PP
- Aloisio A
- Alonso F
- Alpigiani C
- Alshehri AA
- Alvarez Estevez M
- Alviggi MG
- Amaral Coutinho Y
- Ambler A
- Ambroz L
- Amelung C
- Amidei D
- Amor Dos Santos SP
- Amoroso S
- Amrouche CS
- An F
- Anastopoulos C
- Andari N
- Andeen T
- Anders CF
- Anders JK
- Andreazza A
- Andrei V
- Anelli CR
- Angelidakis S
- Angerami A
- Anisenkov A
- Annovi A
- Antel C
- Anthony MT
- Antipov E
- Antonelli M
- Antrim DJA
- Anulli F
- Aoki M
- Aparisi Pozo JA
- Araque JP
- Araujo Ferraz V
- Araujo Pereira R
- Araya ST
- Arcangeletti C
- Arce ATH
- Arduh FA
- Arguin J-F
- Argyropoulos S
- Arling J-H
- Armbruster AJ
- Armstrong A
- Arnaez O
- Arnold H
- Artoni G
- Artz S
- Asai S
- Asawatavonvanich T
- Asbah N
- Asimakopoulou EM
- Asquith L
- Assahsah J
- Assamagan K
- Astalos R
- Astigarraga MEP
- Atkin RJ
- Atkinson M
- Atlay NB
- Atmani H
- Augsten K
- Avolio G
- Ayoub MK
- Azuelos G
- Bachacou H
- Bachas K
- Backes M
- Backman F
- Bagnaia P
- Bahmani M
- Bahrasemani H
- Bailey AJ
- Bailey VR
- Baines JT
- Bakalis C
- Baker OK
- Bakker PJ
- Balaji S
- Baldin EM
- Balek P
- Balli F
- Balunas WK
- Balz J
- Banas E
- Bandyopadhyay A
- Banerjee S
- Bannoura AAE
- Barak L
- Barbe WM
- Barberio EL
- Barberis D
- Barbero M
- Barbour G
- Barillari T
- Barisits M-S
- Barkeloo J
- Barklow T
- Barnea R
- Barnett BM
- Barnett RM
- Barnovska-Blenessy Z
- Baroncelli A
- Barone G
- Barr AJ
- Barreiro F
- Barsov S
- Bartoldus R
- Bartolini G
- Barton AE
- Bartos P
- Basalaev A
- Basan A
- Bassalat A
- Basso MJ
- Bates RL
- Batlamous S
- Batley JR
- Batool B
- Battaglia M
- Bauce M
- Bauer F
- Bauer KT
- Bawa HS
- Beacham JB
- Beau T
- Beauchemin PH
- Becherer F
- Bechtle P
- Beck HC
- Beck HP
- Becker K
- Becot C
- Beddall A
- Beddall AJ
- Bednyakov VA
- Bedognetti M
- Beermann TA
- Begalli M
- Begel M
- Behera A
- Behr JK
- Beisiegel F
- Bell AS
- Bella G
- Bella LA
- Bellagamba L
- Bellerive A
- Bellos P
- Beloborodov K
- Belotskiy K
- Belyaev NL
- Benchekroun D
- Benekos N
- Benhammou Y
- Benjamin DP
- Benoit M
- Bensinger JR
- Bentvelsen S
- Beresford L
- Beretta M
- Berge D
- Berger N
- Bergmann B
- Bergsten LJ
- Beringer J
- Berlendis S
- Berlingen JM
- Bernardi G
- Bernius C
- Bernlochner FU
- Berry T
- Berta P
- Bertella C
- Bertram IA
- Besson N
- Bethani A
- Bethke S
- Betti A
- Bevan AJ
- Beyer J
- Bhattacharya DS
- Bhattarai P
- Bi R
- Bianchi RM
- Biebel O
- Biedermann D
- Bielski R
- Bierwagen K
- Biesuz N
- Biglietti M
- Billoud TR
- Bindi M
- Bingul A
- Bini C
- Biondi S
- Birman M
- Bisanz T
- Biswal JP
- Biswas D
- Bitadze A
- Bittrich C
- Bjorke K
- Blazek T
- Bloch I
- Blocker C
- Blue A
- Blumenschein U
- Bobbink GJ
- Bobrovnikov VS
- Bocchetta SS
- Bocci A
- Boeriu OEV
- Boerner D
- Bogavac D
- Bogdanchikov AG
- Bohm C
- Boisvert V
- Bokan P
- Bold T
- Bolz AE
- Bomben M
- Bona M
- Bonilla JS
- Boonekamp M
- Booth CD
- Borecka-Bielska HM
- Borgna LS
- Borisov A
- Borissov G
- Bortfeldt J
- Bortoletto D
- Boscherini D
- Bosman M
- Bostanabad MG
- Bouaouda K
- Boudreau J
- Bouhova-Thacker E
- Boumediene D
- Bourdarios CA
- Boutle SK
- Boveia A
- Boyd J
- Boye D
- Boyko IR
- Bozson AJ
- Bracinik J
- Brahimi N
- Brandt G
- Brandt O
- Braren F
- Brau B
- Brau JE
- Brendlinger K
- Brenner L
- Brenner R
- Bressler S
- Bret MC
- Brickwedde B
- Briglin DL
- Britton D
- Britzger D
- Brock I
- Brock R
- Brooijmans G
- Brooks WK
- Brost E
- Broughton JH
- Bruncko D
- Bruni A
- Bruni G
- Bruni LS
- Bruno S
- Bruschi M
- Bruscino N
- Bryant P
- Bryngemark L
- Buanes T
- Buat Q
- Buchholz P
- Buckley AG
- Budagov IA
- Buehrer F
- Buescher V
- Bugge MK
- Bulekov O
- Burch TJ
- Burdin S
- Burgard CD
- Burger AM
- Burghgrave B
- Burr JTP
- Burton CD
- Burzynski JC
- Buschmann E
- Bussey PJ
- Butler JM
- Buttar CM
- Butterworth JM
- Butti P
- Buttinger W
- Buzatu A
- Buzykaev AR
- Bylund OB
- Cabras G
- Cabrera Urban S
- Caforio D
- Cai H
- Cairo VMM
- Cakir IT
- Cakir O
- Calace N
- Calafiura P
- Calandri A
- Calderini G
- Calfayan P
- Callea G
- Caloba LP
- Caltabiano A
- Calvente Lopez S
- Calvet D
- Calvet S
- Calvet TP
- Calvetti M
- Camarda S
- Camarero Munoz D
- Camarri P
- Camelia EA
- Cameron D
- Camincher C
- Campana S
- Campanelli M
- Camplani A
- Campoverde A
- Canale V
- Canesse A
- Cantero J
- Cao T
- Cao Y
- Capua M
- Cardarelli R
- Cardillo F
- Carducci G
- Carli I
- Carli T
- Carlino G
- Carlson BT
- Carminati L
- Carney RMD
- Caron S
- Carquin E
- Carra S
- Carter JWS
- Casado MP
- Casha AF
- Casper DW
- Castelijn R
- Castillo Garcia L
- Castillo Gimenez V
- Castillo FL
- Castillo LRF
- Castro NF
- Catinaccio A
- Catmore JR
- Cattai A
- Cavaliere V
- Cavallaro E
- Cavalli-Sforza M
- Cavasinni V
- Celebi E
- Cerda Alberich L
- Cerny K
- Cerqueira AS
- Cerri A
- Cerrito L
- Cerutti F
- Cervelli A
- Cetin SA
- Chadi Z
- Chakraborty D
- Chan J
- Chan WS
- Chan WY
- Chapman JD
- Chargeishvili B
- Charlton DG
- Charman TP
- Chau CC
- Che S
- Chekanov S
- Chekulaev S
- Chelkov GA
- Chen B
- Chen C
- Chen CH
- Chen H
- Chen J
- Chen S
- Chen SJ
- Chen X
- Chen Y-H
- Cheng HC
- Cheng HJ
- Cheplakov A
- Cheremushkina E
- Cheu E
- Cheung K
- Chevalerias TJA
- Chevalier L
- Chiarella V
- Chiarelli G
- Chiodini G
- Chisholm AS
- Chitan A
- Chiu I
- Chiu YH
- Chizhov M
- Choi K
- Chomont AR
- Chouridou S
- Chow YS
- Chu MC
- Chu X
- Chudoba J
- Chwastowski JJ
- Chytka L
- Cieri D
- Ciesla KM
- Cinca D
- Cindro V
- Cioara IA
- Ciocio A
- Cirotto F
- Citron ZH
- Citterio M
- Ciubotaru DA
- Ciungu BM
- Clark A
- Clark MR
- Clark PJ
- Clement C
- Coadou Y
- Cobal M
- Coccaro A
- Cochran J
- Cohen H
- Coimbra AEC
- Cole B
- Colijn AP
- Collot J
- Conde Muino P
- Connell SH
- Connelly IA
- Constantinescu S
- Conventi F
- Cooper-Sarkar AM
- Corga KDV
- Cormier F
- Cormier KJR
- Cornell SD
- Corpe LD
- Corradi M
- Correia AMH
- Corrigan EE
- Corriveau F
- Costa MJ
- Costanza F
- Costanzo D
- Cowan G
- Cowley JW
- Crane J
- Cranmer K
- Crawley SJ
- Creager RA
- Crepe-Renaudin S
- Crescioli F
- Cristinziani M
- Croft V
- Crosetti G
- Cueto A
- Cukierman AR
- Cunningham WR
- Czekierda S
- Czodrowski P
- D'amen G
- D'Amico V
- D'Auria S
- D'Eramo L
- da Costa JBG
- Da Fonseca Pinto J
- Da Via C
- Dabrowski W
- Dachs F
- Dado T
- Dahbi S
- Dai T
- Dallapiccola C
- Dam M
- Damazio DO
- Damp J
- Dandoy JR
- Daneri MF
- Dann NS
- Danninger M
- Dao V
- Darbo G
- Dartsi O
- Dattagupta A
- Daubney T
- David C
- Davidek T
- Davis DR
- Dawson I
- De Asmundis R
- De Beurs M
- De Castro S
- De Cecco S
- De Groot N
- de Jong P
- De la Torre H
- de Lima DEF
- De Lima RT
- De Maria A
- De Pedis D
- De Regie JBDV
- de Renstrom PAB
- De Sa RCL
- De Salvo A
- De Sanctis U
- De Santis M
- De Santo A
- De Sousa MJDCS
- De K
- Debenedetti C
- Dedovich D
- Deiana AM
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- Zanzi D
- Zaripovas DR
- Zeissner S
- Zeitnitz C
- Zemaityte G
- Zeng JC
- Zenin O
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- Zhao Z
- Zhemchugov A
- Zheng Z
- Zhong D
- Zhou B
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- Zhou N
- Zhou Y
- Zhu C
- Zhu CG
- Zhu H
- Zhu HL
- Zhu J
- Zhu Y
- Zhuang X
- Zhukov K
- Zhulanov V
- Zieminska D
- Zimine N
- Zimmermann S
- Zinonos Z
- Ziolkowski M
- Zivkovic L
- Zobernig G
- Zoccoli A
- Zoch K
- Zorbas TG
- Zou R
- Zu Theenhausen HM
- Zwalinski L
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2015
- Field of study
Results of a search for new phenomena in final states with an energetic jet and large missing transverse momentum are reported. The search uses 20.3 fbâ1 of â s = 8 TeV data collected in 2012 with the ATLAS detector at the LHC. Events are required to have at least one jet with pT > 120 GeV and no leptons. Nine signal regions are considered with increasing missing transverse momentum requirements between Emiss T > 150 GeV and Emiss T > 700 GeV. Good agreement is observed between the number of events in data and Standard Model expectations. The results are translated into exclusion limits on models with either large extra spatial dimensions, pair production of weakly interacting dark matter candidates, or production of very light gravitinos in a gauge-mediated supersymmetric model. In addition, limits on the production of an invisibly decaying Higgs-like boson leading to similar topologies in the final state are presente
From Cleanroom to Desktop: Emerging Micro-Nanofabrication Technology for Biomedical Applications
- Author
- A del Campo
- A Grimes
- A Hatch
- A Jamshidi
- A Khademhosseini
- A Khademhosseini
- A Khademhosseini
- A Khademhosseini
- A Ozcan
- A Revzin
- A Revzin
- A Tourovskaia
- AAS Bhagat
- AM Christensen
- AP Quist
- AV Kabashin
- AV Pattekar
- AW Martinez
- AW Martinez
- AW Martinez
- AW Martinez
- AY Fu
- B Bohl
- B Khademhosseinieh
- B Sundaray
- BA Parviz
- BH Jo
- C Hong
- C Li
- C Liu
- C Liu
- C Mao
- CC Fu
- CH Lin
- CS Chen
- D Huh
- D Li
- D Liang
- D Nguyen
- D Pushkarev
- D Qin
- D Sasaki
- D Sun
- D Tseng
- D Yang
- DC Duffy
- DD Carlo
- DK Kim
- DL Englert
- DN Breslauer
- DN Breslauer
- DS Malkin
- DY Stevens
- E Berthier
- E Carrilho
- E Carrilho
- E Fu
- E Tkachenko
- EP Chan
- F Umbrecht
- FG Omenetto
- G Altman
- G Braun
- GA Ozin
- GB Lee
- GE Moore
- GJ Kost
- GM Walker
- GM Walker
- GM Whitesides
- GM Whitesides
- GP Payne
- GV Kaigala
- H Cong
- H Cong
- H Gu
- H Hwang
- H Hwang
- H Li
- H Yan
- H Yi
- HA Becerril
- HC Moeller
- HY Hsu
- I Barbulovic-Nad
- J Atencia
- J Bath
- J Carlier
- J Friend
- J Ju
- J Kobayashi
- J Steigert
- J Xie
- J Zhou
- J Zhou
- JC McDonald
- JC Mohr
- JL Osborn
- JL Tan
- JM Curran
- JM Karp
- JP Beech
- JR Anderson
- JR Rettig
- JS Go
- JY Lee
- K Haubert
- K Itoga
- K Itoga
- K Itoga
- K Itoga
- K Khare
- K Najafi
- K Sollier
- K Stephan
- KD Wise
- KD Wise
- KE Nelson
- KL Mills
- KS Lee
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- LJ Zhang
- LT Jiang
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- M De
- M Mastrangeli
- M Natali
- M Rafat
- M Wang
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- MJ Banholzer
- MJ Madou
- ML Berre
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- N Bowden
- P Abgrall
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- P Kauffman
- P Kim
- P Tseng
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- PWK Rothemund
- PY Chiou
- R Delille
- R Derda
- R Jayakumar
- R Khnouf
- RA Petros
- RD Piner
- RJ Aitken
- RJ Tseng
- RW Sands
- S Bhattacharya
- S Chung
- S Li
- S Park
- S Seo
- S Zhao
- SA Ruiz
- SK Seidlits
- SP Desai
- SR Quake
- SS Agasti
- SS Shah
- T Koerner
- T Pan
- Tingrui Pan
- TM Keenan
- UY Schaff
- UY Schaff
- V Vogel
- VI Vullev
- W Choi
- W Wang
- Wei Wang
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- WH Ko
- X Cui
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- X Qian
- XZ Niu
- Y Cui
- Y Cui
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- Y Li
- Y Zeng
- Y Zeng
- YC Tsai
- YC Tsai
- YM Shin
- YP Zhao
- YZ Wang
- YZ Wu
- Z Chen
- Z Chen
- Publication venue
- Springer US
- Publication date
- 01/01/2010
- Field of study
This review is motivated by the growing demand for low-cost, easy-to-use, compact-size yet powerful micro-nanofabrication technology to address emerging challenges of fundamental biology and translational medicine in regular laboratory settings. Recent advancements in the field benefit considerably from rapidly expanding material selections, ranging from inorganics to organics and from nanoparticles to self-assembled molecules. Meanwhile a great number of novel methodologies, employing off-the-shelf consumer electronics, intriguing interfacial phenomena, bottom-up self-assembly principles, etc., have been implemented to transit micro-nanofabrication from a cleanroom environment to a desktop setup. Furthermore, the latest application of micro-nanofabrication to emerging biomedical research will be presented in detail, which includes point-of-care diagnostics, on-chip cell culture as well as bio-manipulation. While significant progresses have been made in the rapidly growing field, both apparent and unrevealed roadblocks will need to be addressed in the future. We conclude this review by offering our perspectives on the current technical challenges and future research opportunities
A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)
- Author
- Abbott TE
- Abdallah RI
- Abdel-Aal IR
- Abdelmonem SA
- Abdo WF
- Abellan AN
- AbellĂĄn AN
- AbellĂĄn AN
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- AKI Research Group
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- Imanaka H
- Inaloo S
- Ince C
- Iotti GA
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- Iotti GA
- IPREA Study Group
- Irazabal JM
- Irazabal JM
- Ishimatsu S
- Itenov TS
- Iwamoto M
- Izawa M
- Izdes S
- Jaber S
- Jacky A
- Jacobs FM
- Jacques T
- Jaffal K
- Jamali S
- Jang BH
- Janotka M
- Janotka M
- Janotka M
- Jansen D
- Jansen N
- Japan Septic Disseminated Intravascular Coagulation (JSEPTIC DIC) study group
- Jardim JJ
- Jardim M
- Jareño A
- Javadpour S
- Jayasinghe S
- Jean-Baptiste S
- Jean-Baptiste S
- Jensen AE
- Jensen JU
- Jeon YD
- Jeon YD
- Jeong IY
- Jeong IY
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- Jeong WY
- Jian Z
- Jiao LR
- Jibaja M
- Jimenez-Herrera MF
- Jiménez GJ
- Jo YH
- Joachim J
- Jochmans S
- John G
- Jonas M
- Jonas M
- Jonas M
- Jones C
- Joshi R
- Joshi V
- Jovaisa T
- JSEPTIC (Japanese Society of Education for Physicians and Trainees in Intensive Care) Clinical Trial Group
- Juan WC
- Juanas CA
- Judickas S
- Juez A
- Juliarena A
- Jun IG
- Jun IG
- Jung K
- Jung KW
- Junior JM
- Kaczirek K
- Kaese S
- Kalani N
- Kalenka A
- Kalfon P
- Kamali E
- Kaminsky GE
- Kaneko M
- Kang M
- Kang M
- Kang PL
- Kang PL
- Kang PL
- Kang PL
- Kang Y
- Kao KC
- Kappler F
- Kara A
- Karachi F
- Karanjia N
- Karbing DS
- Karsten E
- Kasdan H
- Kashiya S
- Kashyap R
- Katabami K
- Katabami K
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- Katabami K
- Katira BH
- Kavanagh BP
- Kawamura N
- Kawano S
- Kay FU
- Kayaaslan B
- Kayambu G
- Kazutoshi F
- Ke MW
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- Keep J
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- Kellner F
- Kelly JM
- Kenardy J
- Keough E
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- Kerr C
- Kezyte G
- Khadjibaev A
- Khaliq W
- Khine H
- Kim DJ
- Kim HJ
- Kim JM
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- Kim KS
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- Kim MH
- Kim MH
- Kim W
- Kimmoun A
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- Kinnear J
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- Kirakli C
- Kirca H
- Kirman M
- Kirwan CJ
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- Klapsing P
- Klaus DA
- Kleinpell R
- Kluge S
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- Ko JI
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- Kobayashi A
- Koco E
- Kodate A
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- Kokkini S
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- Komuro T
- Kondili E
- Kongpolprom N
- Kooner G
- Kostalas M
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- Kou PS
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- Kovacikova L
- Kox M
- Kraegpoeth A
- Krenn CG
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- Kristof J
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- Kubik M
- Kubota K
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- Kunze-Szikszay N
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- Kurtz P
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- Kwon HM
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- Kye YC
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- Labaune MA
- Labra C
- Lacerda P
- Laerkner E
- Lafaurie M
- Lafuente E
- Lagonidis D
- Lagos R
- Lahmer T
- Lai CC
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- Latini R
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- Latorre J
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- Lichtenstein D
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- Lukaszewicz AC
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- Maistry N
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- Morman D
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- Motos A
- Mouaci A
- Mourad M
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- Moxham J
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- Mukherjee DN
- Muncharaz AB
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- Spronk PE
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- St Georgeâs University of London
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- Ălvarez JT
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- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2016
- Field of study
Meeting abstrac
Multi-messenger observations of a binary neutron star merger
- Author
- Aab A
- Aartsen MG
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- Mazumder N
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- Michelson PF
- Middendorf L
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- Milano L
- Milia Sabrina
- Miller AA
- Miller CJ
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- Mitselmakher G
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- Mohan M
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- Mohr JJ
- Mohrmann L
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- Moment G
- Montanet F
- Montani M
- Montaruli T
- Monzani ME
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- Moore CJ
- Moore RW
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- Morello C
- Moreno E
- Moreno G
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- Morita K
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- Morlino G
- Morokuma T
- Morris D
- Morriss SR
- Morselli A
- Morselli A
- Moskalenko IV
- Moskvitin AS
- Mostaf M
- Motohara K
- Moulai M
- Moulin E
- Mours B
- Moussa A
- Mow-Lowry CM
- Mrka S
- Mrka Z
- Mueller AL
- Mueller AL
- Mueller BB
- Mueller G
- Mueller G
- Mueller MA
- Mueller S
- Muir AW
- Muir J
- Mukherjee Arunava
- Mukherjee D
- Mukherjee S
- Mukund N
- Mullavey A
- Munar-Adrover P
- Munch J
- Mundell CG
- Muniz EA
- Muoz A Gonzolez
- Muoz RR
- Murach T
- Murata KL
- Muratore M
- Murguia-Berthier A
- Murphy T
- Murray PG
- Mussa R
- Myers ST
- Nagai H
- Nagashima H
- Nagayama T
- Nahnhauer R
- Nakahira S
- Nakajima M
- Nakamura Y
- Nakaoka T
- Nakar E
- Nakarmi P
- Nakashima S
- Nakata F
- Nang Y
- Napier K
- Naranjo I
- Nardecchia I
- Natalucci L
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- Naumann U
- Nava L
- Navarrini Alessandro
- Navas S
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- Nayak RK
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- Negro M
- Neilsen E
- Neilson J
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- Nellen L
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- Newbold M
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- Nolting D
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- North C
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- Nousek JA
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- Nowicki SC
- Noysena K
- Nozka L
- Nugent P
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- Nuss E
- Nuttall LK
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- Nynka M
- O'Brien P
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- O'Shaughnessy R
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- Oda S
- Odaka H
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- Oh JJ
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- Ohm S
- Ohme F
- Ohsawa R
- Ohshima T
- Ohta K
- Oikonomou F
- Ojha R
- Okada MA
- Okita H
- Oleynik Ph P
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- Olivas A
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- Onken CA
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- Opiela R
- Oppermann P
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- Orange B
- Organokov M
- Orienti M
- Orlando E
- Orlati A
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- Ortega LF
- Ortu P
- Osborne JP
- Oslowski S
- Ossokine S
- Ostrowski M
- Ottaway DJ
- Ou G
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- Owen BJ
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- Ozawa S
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- Pace AE
- Paciesas WS
- Padilla N
- Padovani M
- Pagani C
- Page J
- Page KL
- Page MA
- Pai A
- Pai SA
- Pal-Singh A
- Palamos JR
- Palashov O
- Palatiello M
- Palatka M
- Palazzi E
- Palczewski T
- Paliya VS
- Palliyaguru N
- Palliyaguru NT
- Pallot D
- Pallotta J
- Palmas S
- Palmer DM
- Palmerio JT
- Palmese A
- Palomba C
- Pan Howard
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- Pandey SB
- Pandya H
- Paneque D
- Pang B
- Pang PTH
- Pankova DV
- Pankow C
- Pannarale F
- Pant BC
- Panter M
- Paoletti F
- Paoletti F
- Paoli A
- Papa MA
- Papenbreer P
- Paragi Z
- Parente G
- Parida A
- Park IH
- Parker W
- Parkinson PM Saz
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- Parra A
- Parsons RD
- Pascucci D
- Pasqualetti A
- Passaquieti R
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- Pastorello A
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- Pathfinder ASKAP Australian SKA
- Patil M
- Patricelli B
- Paul T
- Pavalas GE
- Pavana M
- Paxman J
- Paz-Chinchn F
- Pea-Rodriguez J
- Pearlstone BL
- Pech M
- Pedraza M
- Pedreira F
- Pedurand R
- Peiffer P
- Peixoto CJ Todero
- Pekeur NW
- Pekowsky L
- Pelayo R
- Pele A
- Pellegrino C
- Pelletier G
- Pellizzoni A
- Penacchioni AV
- Penn S
- Penuela T
- Pepper JA
- Pereira LAS
- Perennes C
- Perez CJ
- Perez-Perez EG
- Perley DA
- Perlin M
- Perreca A
- Perri LM
- Perri M
- Perrina C
- Perrodin D
- Perrone L
- Pescalli A
- Pesce-Rollins M
- Peters C
- Peters WM
- Petrera S
- Petrucci P-O
- Peyaud B
- Pfeiffer HP
- Phelps M
- Phlhofer G
- Phuntsok J
- Pian E
- Piano G
- Piattelli P
- Piccinni OJ
- Pichot M
- Picozza P
- Piel Q
- Pieloth D
- Piergiovanni F
- Pierog T
- Pierro V
- Pignata G
- Pike S
- Pilia M
- Pillant G
- Pimenta M
- Pinard L
- Pinat E
- Pinto IM
- Piotrowski LW
- Piran T
- Piran T
- Piranomonte S
- Pirello M
- Piro AL
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- Piron F
- Pirronello V
- Pisanu T
- Pita S
- Pitkin M
- Pittori C
- Placco VM
- Platino M
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- Plum M
- Podesta F
- Podesta RC
- Podsiadlowski P
- Poe M
- Poggiani R
- Poh J
- Poireau V
- Pollmann A Obertacke
- Poolakkil S
- Poon H
- Popa V
- Popolizio P
- Poppi S
- Porowski C
- Porter EK
- Porter TA
- Possenti A
- Post A
- Potter SB
- Powell J
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- Poznanski D
- Pradier T
- Prado RR
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- Preece RD
- Prentice SJ
- Prestegard T
- Pretz J
- Price LR
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- Prijatelj M
- Principe G
- Principe M
- Pritchard TA
- Privitera P
- Privitera S
- Prochaska JX
- Prodi GA
- Prokhorov D
- Prokhorov LG
- Prokoph H
- Prouza M
- Prrer M
- Przybylski GT
- Pulone L
- Pumo ML
- Punch M
- Puncken O
- Punturo M
- Puppo P
- Qi H
- Qi H
- Qu JL
- Quataert E
- Quel EJ
- Querchfeld S
- Querel R
- Quetschke V
- Quimby RM
- Quinn L
- Quinn S
- Quinones C
- Quintero EA
- Quirrenbach A
- Quispe ID Vergara
- Quitzow-James R
- Raab C
- Raab FJ
- Raab S
- Rabeling DS
- Rabus M
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- Racusin J
- Radkins H
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- Rain S
- Raja S
- Raja W
- Rajan C
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- Rameez M
- Ramirez KE
- Ramirez-Ruiz E
- Ramos-Buades A
- Ramos-Pollan R
- Rana J
- Rando R
- Rao AR
- Rapagnani P
- Rappoldi A
- RATIR RIMAS
- Rau A
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- Raymond V
- Razza A
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- Razzano M
- Razzaque S
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- Rdiger A
- Rea IC
- Read J
- Rebolo R
- Regimbau T
- Rei L
- Reichart DE
- Reid S
- Reimann R
- Reimer A
- Reimer A
- Reimer O
- Reimer O
- Reitze DH
- Relethford B
- Relich M
- Ren W
- Ren Z
- Renaud M
- Renzi V
- Reposeur T
- Resconi E
- Resmi L
- Rest A
- Reva IV
- Reyes R De Los
- Reyes SD
- Reynolds T
- Rho CD
- Rhode W
- Ribeiro T
- Ricci F
- Ricci R
- Ricciarini S
- Riccobene G
- Richman M
- Ricker PM
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- Ridky J
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- Rios-Lopez E
- Risse M
- Ristori P
- Rivera A Ramirez
- Riviere C
- Rizi V
- Rizzo M
- Roberts OJ
- Robertson NA
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- Robie R
- Robinet F
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- Rol E
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- Rosa-Gonzlez D
- Rosell A Carnero
- Rosenberg M
- Rosetti S
- Rosinska D
- Ross MP
- Rossi A
- Rosswog S
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- Roth M
- Rott C
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- Rovero AC
- Rowan S
- Rowell G
- Rowlinson A
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- Roy R
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- Rulten CB
- Rumyantsev VV
- Rusu F
- Rutins G
- Ryan K
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- Rykoff ES
- Rysewyk D
- Saba A
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- Sadeghian L
- Sadler EM
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- Saffe C
- Saffi SJ
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- Sahakian V
- Sai N
- Saito S
- Saito T
- Saito Y
- Sakamaki A
- Sakamoto T
- Sakellariadou M
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- Salamida F
- Salazar H
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- Saleem M
- Saleh A
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- Salina G
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- Salvadori I
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- Sanchez B
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- Sanchez LE
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- Sanchis-Gual N
- Sand DJ
- Sandberg V
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- Sandoval A
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- Sapienza P
- Sarazin F
- Sarkar S
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- Sarmento R
- Sarmiento-Cano C
- Sasaki M
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- Sassolas B
- Satalecka K
- Sathyaprakash BS
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- Saulson PR
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- Scheuer J
- Schieler H
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- Schindler R
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- Schlegel DJ
- Schlickeiser R
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- Schneider A
- Schneider M
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- Schoenen S
- Schofield RMS
- Scholten O
- Schonbeck A
- Schoneberg S
- Schoorlemmer H
- Schovnek P
- Schreiber E
- Schroder FG
- Schroder S
- Schroder SL
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- Schulz A
- Schulz A
- Schulze S
- Schumacher J
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- Schutz BF
- Schwalbe SG
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- Schweyer T
- Sciutto SJ
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- Scott J
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- Segreto A
- Seidel E
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- Sekiguchi Y
- Sellers D
- Selsing J
- Sengupta AS
- Sentenac D
- Sequino V
- Sergeev A
- Sergienko Yu P
- Serino M
- Serra G
- Serra-Ricart M
- Serrau M
- Settimo M
- Seunarine S
- Sevilla-Noarbe I
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- Shaffer TJ
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- Shidatsu M
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- Siemens X
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- Sigg D
- Sigl G
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- Steinlechner J
- Steinlechner S
- Steinmeyer D
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- Suarez F
- Suarez-Duran M
- Suchyta E
- Sudholz T
- Sugawara Y
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- Tacca M
- Tachibana Y
- Taddia F
- Tagliaferri G
- Tait SC
- Taiuti M
- Tajitsu A
- Takahashi I
- Takahashi J
- Takahashi M
- Takahashi T
- Takao Y
- Takayama M
- Talbot C
- Talukder D
- Tamura T
- Tamura Y
- Tan Y
- Tanaka I
- Tanaka M
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- Tanimoto A
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- Tao C
- Tao L
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- Taracchini A
- Tarle G
- Tartaglia L
- Tasson JD
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- Ter Veen S
- Ter-Antonyan S
- Terai T
- Terliuk A
- Terreran G
- Terrier R
- Tesic G
- Testa V
- Tewari SV
- Thayer JB
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- Theeg T
- Thene CC
- Thierry P
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- Thies F
- Thomas EG
- Thomas M
- Thomas P
- Thomas RC
- Thompson DJ
- Thorne KA
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- Thrane E
- Tibaldo L
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- Tibolla O
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- Timmermans C
- Tingay SJ
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- Tiwari V
- Tiziani D
- Tjus J Becker
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- Tlatov AG
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- Tokmakov KV
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- Toma K
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- Tonelli M
- Tonnis C
- Tonry J
- Torii S
- Tornasi Z
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- Torres DF
- Torres I
- Torres-Flores S
- Torres-Forn A
- Torresi E
- Torrie CI
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- Tosi D
- Towner MC
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- Tpai M
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- Travnicek P
- Traylor G
- Trichard C
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- Trinastic J
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- Troxel MA
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- Tsunemi H
- Tsvetkova AE
- Tucker BE
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- Turpin D
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- Publication venue
- 'American Astronomical Society'
- Publication date
- 01/01/2017
- Field of study
On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ~1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40+8-8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 Mo. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ~40 Mpc) less than 11 hours after the merger by the One- Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ~10 days. Following early non-detections, X-ray and radio emission were discovered at the transientâs position ~9 and ~16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta
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- Abdel-Aziz AK
- Abdelfatah S
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- Zhong Q
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- Zhu B
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- Zhuang H
- Zhuang X
- Zientara-Rytter K
- Zimmermann CM
- Ziviani E
- Zoladek T
- Zong W-X
- Zorov DB
- Zorzano A
- Zou W
- Zou Z
- Zou Z
- Zuryn S
- Zwerschke W
- Ălvarez ĂMC
- Ăvalos Y
- Ănal G
- ĂstĂŒn S
- ÄoliÄ M
- ÄokiÄ J
- Ćœerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
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