Uso da fish em mucosa oral para investigação de mosaicismo com linhagem 45,x: estudo com homens saudáveis e pacientes com distúrbios da diferenciação do sexo

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOObjective: To verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Subjects and methods: Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. Results: The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Conclusions: Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium. © ABE&M todos os direitos reservados.To verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Subjects and methods: Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. Results: The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Conclusions: Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium584328334FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO2011/50189-7Verificar se a hibridização in situ por fluorescência (FISH) em células de mucosa oral poderia ser empregada para detectar criptomosaicismo com linhagem 45,X em pacientes 46,XY. Sujeitos e métodos: Amostra de 19 jovens saudáveis 46,XY e cinco pacientes com distúrbios da diferenciação do sexo (DDS), quatro 45,X/46,XY e um 46,XY. FISH com sondas específicas para X e Y em núcleos interfásicos de linfócitos e mucosa oral para investigar aproporção de núcleos contendo apenas o sinal do cromossomo X. Resultados: A frequência de núcleos contendo apenas o sinal do X nos dois tecidos dos homens saudáveis não diferiu (p =0,69). Em todos os pacientes com DDS essa frequência foi significativamente maior, e tambémnão houve diferença entre os dois tecidos (p = 0,38). Conclusões: A investigação de mosaicismo com linhagem 45,X em pacientes com DDS 46,XY ou esterilidade pode ser feita por FISHdiretamente em células de mucosa ora

Insight into stability of CotA laccase from the spore coat of Bacillus subtilis

The axial ligand of the catalytic mononuclear T1 copper site (Met(502)) of the CotA laccase was replaced by a leucine or phenylalanine residue to increase the redox potential of the enzyme. These mutations led to an increase in the redox potential by approx. 100 mV relative to the wild-type enzyme but the catalytic constant k(cat) in the mutant enzymes was severely compromised. This decrease in the catalytic efficiency was unexpected as the X-ray analysis of mutants has shown that replacement of methionine ligand did not lead to major structural changes in the geometry of the T1 Centre or in the overall fold of the enzyme. However, the mutations have a profound impact on the thermodynamic stability of the enzyme. The fold of the enzyme has become unstable especially with the introduction of the larger phenylalanine residue and this instability should be related to the decrease in the catalytic efficiency. The instability of the fold for the mutant proteins resulted in the accumulation of an intermediate state, partly unfolded, in-between native and unfolded states. Quenching of tryptophan fluorescence by acrylamide has further revealed that the intermediate state is partly unfolded.info:eu-repo/semantics/publishedVersio

Produtividade de raízes de mandioca consorciada com milho e caupi em sistema orgânico.

Foram avaliados sistemas orgânicos de produção de mandioca “de mesa”, em Seropédica (RJ). O experimento constou dos seguintes tratamentos: monocultivo de mandioca (cv. IAC 576-70) e consórcios com milho experimental (cv. Eldorado), caupi (cv. Mauá) e milho+caupi. O manejo orgânico foi padronizado e toda a área experimental irrigada durante o período de permanência do milho no sistema. Do milho, foram colhidas espigas verdes (imaturas) e a parte aérea acamada na superfície do solo. O caupi foi incluído como adubo verde e cortado na floração, sendo mantidos os resíduos na superfície do solo. Ambos os consortes ocuparam as entrelinhas da mandioca, de modo alternado, semeados após a primeira capina da cultura principal. A cultivar IAC 576-70 mostrou-se adaptada ao manejo orgânico, com produtividade de raízes de padrão comercial próxima a 31 Mg ha-¹. Não houve diferenças significativas entre o monocultivo e os três tipos de consórcios testados. A inclusão do milho representou potencial de renda adicional ao produtor, colhendo-se,em média, 18.125 espigas ha-¹, o que correspondeu a 5,1 Mg ha-¹. Os resíduos provenientes da roçada do caupi proporcionaram um aporte de biomassa fresca de 12 Mg ha-¹, com uma expressiva contribuição em nitrogênio (cerca de 44 kg de N ha-1). A fabácea leguminosa cobriu por completo as entrelinhas da mandioca,demonstrando seu potencial de controle à erosão e a ervas espontâneas. O consórcio triplo mostrou-se vantajoso tendo em vista que a receita obtida com a venda do milho verde justificaria os custos da irrigação, além dos benefícios da inclusão do caupi e da não interferência dos consortes na produtividade da mandioca

Carbono orgânico dissolvido e biodisponibilidade de N e P como indicadores de qualidade do solo

Nas últimas décadas, qualidade do solo tem se tornado um tópico importante na ciência do solo. Embora esforços consideráveis tenham sido dedicados com o intuito de definir "qualidade do solo", ainda não há um conceito amplamente aceito pela comunidade cientifica. A seleção de índices qualitativos para definir qualidade do solo é uma tarefa extremamente difícil, e diversas propriedades químicas, físicas e biológicas tem sido sugeridas como potenciais indicadores. A matéria orgânica do solo está associada com processos químicos, físicos e biológicos no solo, e, portanto, é considerada um dos melhores indicadores de qualidade do solo. O manejo do solo pode influenciar significativamente a dinâmica do carbono orgânico e o ciclo de N, P, e S. Entretanto, mudanças na concentração total da matéria organica em resposta ao manejo pode ser dificil de ser detectada devido à variabilidade natural do solo. Quando comparada com a matéria orgânica total do solo, a fração mais prontamente disponível, como o carbono orgânico dissolvido (COD), é mais sensível às mudanças no manejo do solo a curto e médio prazo e, portanto, pode ser utilizada como indicador fundamental de qualidade do solo ou das alterações das condições naturais. Embora a fração dissolvida represente apenas uma pequena porção da matéria orgânica total do solo, o COD é móvel no solo e constitui uma importante fonte de C para os microorganismos, podendo facilmente refletir os efeitos de diferentes sistemas de manejo. Inúmeros métodos são utilizados para caracterizar o COD, mas os processos que influenciam sua mineralização e a disponibilidade dos elementos associado com a matéria orgânica (N, P, e S) ainda não são completamente entendidos. Pesquisas futuras devem buscar entender os processos que governam a dinâmica de nutrientes e do COD e como os mesmos afetam a qualidade do solo.Soil quality has become an important issue in soil science. Considerable attempts have been made to define soil quality, but a general concept has not yet been accepted by the scientific community. The selection of quantitative indices for soil quality is extremely difficult, and a considerable number of chemical, physical, and biochemical properties have been suggested as potential indicators of soil quality. Because soil organic matter (SOM) can be associated with different soil chemical, physical and biological processes, it has been widely considered as one of the best soil quality indicator. Land use can significantly influence dynamics of organic carbon and N, P, and S cycle. However, changes in total soil organic carbon (SOC) contents in response to land use may be difficult to detect because of the natural soil variability. In the short to medium term, biological properties and readily decomposable fractions of SOC, such as dissolved organic carbon (DOC), are much more sensitive to soil management than is SOM as a whole, and can be used as a key indicator of soil natural functions. Despite the fact that labile C accounts for a small portion of the total organic matter in the soils, DOC is the most mobile and important C-source for microorganisms, and can easily reflect the effects of land use on soil quality. Although several methods are used to characterize DOC, the factors influencing mineralization and bioavailability of elements associated with organic matter (N, P, and S) remains unclear. Future research should focus on the processes that govern DOC and nutrient dynamics and how they affect soil quality

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02