The Play Behaviours of Roma Children in Transylvania

The Roma children of Transylvania are probably the most materially deprived in Europe. They often live in one-room shacks made from wood and mud, with no running water, no sanitation, and sometimes no heating. Many rely on charity for their food and medicines. But, are they play deprived? This paper summarises an observational study of the play behaviours of children in a small Roma village. It highlights the striking contrast between the abject poverty that characterises their lives and the general happiness of the children. These children live their limited lives to the full. They ‘play everywhere and with everything’, but not in the generally accepted sense of that phrase. The usual niceties of privacy, personal possessions and property boundaries are irrelevant here. Their play is rich in imagination and creativity; it is living proof of Nicholson’s theory of loose parts

Painting with Bob:Assisted creativity for novices

Current digital painting tools are primarily targeted at professionals and are often overwhelmingly complex for use by novices. At the same time, simpler tools may not invoke the user creatively, or are limited to plain styles that lack visual sophistication. There are many people who are not art professionals, yet would like to partake in digital creative expression. Challenges and rewards for novices differ greatly from those for professionals. In this paper, we leverage existing works in Creativity and Creativity Support Tools (CST) to formulate design goals specifically for digital art creation tools for novices. We implemented these goals within a digital painting system, called Painting with Bob. We evaluate the efficacy of the design and our prototype with a user study, and we find that users are highly satisfied with the user experience, as well as the paintings created with our system

'The terrible twos': Gaining control in the nursery?

'The terrible twos' are often described as a time of 'gaining control', usually thought of as adults asserting control over children, who learn to control themselves. However, toddlerhood is as much about children learning to take control for themselves. This paper is an attempt to detail something of the social geography in the toddler room of a Scottish nursery, considering both styles of adult control and the ways in which toddlers attempt to appropriate and reconfigure space and time for themselves. That is, the ways in which space and time are negotiated in the course of day-to-day nursery life

Baculovirus Infection Triggers a Shift from Amino Acid Starvation-Induced Autophagy to Apoptosis

Autophagy plays a central role in regulating important cellular functions such as cell survival during starvation and control of infectious pathogens. On the other hand, many pathogens have evolved mechanisms of inhibition of autophagy such as blockage of the formation of autophagosomes or the fusion of autophagosomes with lysosomes. Baculoviruses are important insect pathogens for pest control, and autophagy activity increases significantly during insect metamorphosis. However, it is not clear whether baculovirus infection has effects on the increased autophagy. In the present study, we investigated the effects of the Autographa californica nucleopolyhedrovirus (AcMNPV) infection on autophagy in SL-HP cell line from Spodoptera litura induced under amino acid deprivation. The results revealed that AcMNPV infection did not inhibit autophagy but triggered apoptosis under starvation pressure. In the early stage of infection under starvation, mitochondrial dysfunction was detected, suggesting the organelles might be involved in cell apoptosis. The semi-quantitative PCR assay revealed that the expression of both p35 and ie-1 genes of AcMNPV had no significant difference between the starved and unstarved SL-HP cells. The western blot analysis showed that no cleavage of endogenous Atg6 occurred during the process of apoptosis in SL-HP cells. These data demonstrated that some permissive insect cells may defend baculovirus infection via apoptosis under starvation and apoptosis is independent of the cleavage of Atg6 in SL-HP cells

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

red, green and yellow lentil whole flour

Gluten-free lentil tarhanas were obtained by replacing red, green and yellow lentil whole flour instead of wheat flour used in traditional tarhana formulation. Some physical, chemical, bioactive, microbiological, reological, morphological and sensory properties of these tarhanas were investigated comparatively. Amount of crude protein (%), crude ash (%), crude fat(%), soluble-insoluble-total dietary fibre (%), some elements, antioxident activity (mu mol TE/100 g) and total phenolic content (mg GAE/100g) of the tarhanas which were obtained by using lentil flour were significantly increased compared to the control tarhanas. All varieties of tarhana exhibited pseudoplastic flow behavior. In terms of microbiological evaluation; lentil tarhanas has higher lactic acid bacteria with yeast and mold amount compared to control tarhanas. When lentil tarhanas were compared, the green lentil tarhanas is the richest one according to the values of soluble-insoluble-total dietary fiber (%), crude ash (%),total phenolic content and antioxidant activity. Tarhanas examined by scanning electron microscopy (SEM), the control and red lentil tarhanas with green and yellow lentil tarhanas exhibited similar morphological characteristics. It has been found that the sensory evaluation of tarhanas is not statistically different from each other in terms of general appreciation. As a result of this, it shows that gluten-free tarhanas produced with lentil flour can be consumed instead of control tarhanas.C1 [Goencue, Ali] Adnan Menderes Univ, Food Technol Program, Food Proc Dept, Cine Vocat Sch, Aydin, Turkey.[Celik, Ilyas] Pamukkale Univ, Fac Engn, Food Engn Dept, Denizli, Turkey

Pretend Play as a Life-Span Activity: Play, Games and Philosophy [Special Issue]

This article has no associated abstract

Rye and oat flour enriched baked wheat chips: bioactive and textural properties

This study investigates the effect of rye and oat flour incorporation into baked wheat chip formulation on the bioactive, textural and sensory attributes. The increase in the ratio of rye and oat flours ranging from 0-50% resulted in gradual increase in both total phenolic content and diphenylpicrylhydrazyl radical scavenging activity and the chip formulation of 50% wheat+50% oat had the highest values. Texture as assessed hardness value indicated that rye and oat flours addition leads to much more compact structure as compared to 100% wheat flour. Sensory analysis revealed that the samples containing rye and oat flour are acceptable as proved by their scores. It is therefore may be reasonable to fortify the wheat flour formulation with rye/oat flour to produce and health beneficial bakery product

Fibromyalgia, physical and psychological aspects: 107 cases

Fibromyalgia syndrome which is characterized by, widespread musculoskeletal pain, sleep disturbance, fatigue, morning stiffness and tenderness at specific sites is frequently associated with affective dysfunction. The aim of this study is to evaluate patients with fibromyalgia both physically and psychologically. Patients with fibromyalgia syndrome, who were admitted to Cukurova University Faculty of Medicine, Department of Physical Medicine and Rehabilitation are studied. Of the 107 patients, 96 were female and 11 male. In all patients, tender points, factors modulating the symptoms, associated features, laboratory and radiologic findings were determined. We performed Turkish version of Maudsley Obsessional-Compulsive Questionnaire and Zung's Self Rating Depression Scale in patients to evaluate them psychologically. In our study group, the number of tender points varied between 11 and 18. We observed the folio wing associated disturbances: sleep disturbance in 65%, fatigue in 93%, morning stiffness in 72%, subjective paresthesias in 75%, subjective soft tissue swelling in 45%, anxiety in 57%, headache in 73%, irritable bowel syndrome in 43%, widespread pain in 42%, sicca symptoms in 18%, urinary urgency in 24% and dysmenorrhea in 23% (rates). The psychological evaluation of the patients revealed that 43% of them had depressive symptoms and 25% had obsessive-compulsive sings. In conclusion we demonstrated that, besides physical findings, fibromyalgia syndrome is also associated with several psychological symptoms