Interdiffusion and Doping Gradients at the Buffer/Absorber Interface in Thin-Film Solar Cells

An accurate determination of the net dopant concentration in photovoltaic absorbers is critical for understanding and optimizing solar cell performance. The complex device structure of multilayered thin-film solar cells poses challenges to determine the dopant concentration. Capacitance-voltage ( C- V) measurements of Cu(In,Ga)Se2 thin-film solar cells typically yield depth-dependent apparent doping profiles and are not consistent with Hall measurements of bare absorbers. We show that deep defects cannot fully explain these discrepancies. We instead find that the space charge region capacitance follows the model of a linearly graded junction in devices containing a CdS or Zn(O,S) buffer layer, indicating that elemental intermixing at the absorber/buffer interface alters the dopant concentration within the absorber. For absorbers covered with MgF2, C- V measurements indeed agree well with Hall measurements. Photoluminescence measurements of Cu(In,Ga)Se2 absorbers before and after deposition of a CdS layer provide further evidence for a significant reduction of the near-surface net dopant concentration in the presence of CdS. We thus demonstrate that interdiffusion at the absorber/buffer interface is a critical factor to consider in the correct interpretation of doping profiles obtained from C- V analysis in any multilayered solar cell and that the true bulk dopant concentration in thin-film devices might be considerably different

Comparative Structural Analysis of Human DEAD-Box RNA Helicases

DEAD-box RNA helicases play various, often critical, roles in all processes where RNAs are involved. Members of this family of proteins are linked to human disease, including cancer and viral infections. DEAD-box proteins contain two conserved domains that both contribute to RNA and ATP binding. Despite recent advances the molecular details of how these enzymes convert chemical energy into RNA remodeling is unknown. We present crystal structures of the isolated DEAD-domains of human DDX2A/eIF4A1, DDX2B/eIF4A2, DDX5, DDX10/DBP4, DDX18/myc-regulated DEAD-box protein, DDX20, DDX47, DDX52/ROK1, and DDX53/CAGE, and of the helicase domains of DDX25 and DDX41. Together with prior knowledge this enables a family-wide comparative structural analysis. We propose a general mechanism for opening of the RNA binding site. This analysis also provides insights into the diversity of DExD/H- proteins, with implications for understanding the functions of individual family members

Transcriptome-Based Network Analysis Reveals a Spectrum Model of Human Macrophage Activation

SummaryMacrophage activation is associated with profound transcriptional reprogramming. Although much progress has been made in the understanding of macrophage activation, polarization, and function, the transcriptional programs regulating these processes remain poorly characterized. We stimulated human macrophages with diverse activation signals, acquiring a data set of 299 macrophage transcriptomes. Analysis of this data set revealed a spectrum of macrophage activation states extending the current M1 versus M2-polarization model. Network analyses identified central transcriptional regulators associated with all macrophage activation complemented by regulators related to stimulus-specific programs. Applying these transcriptional programs to human alveolar macrophages from smokers and patients with chronic obstructive pulmonary disease (COPD) revealed an unexpected loss of inflammatory signatures in COPD patients. Finally, by integrating murine data from the ImmGen project we propose a refined, activation-independent core signature for human and murine macrophages. This resource serves as a framework for future research into regulation of macrophage activation in health and disease

Height and body-mass index trajectories of school-aged children and adolescents from 1985 to 2019 in 200 countries and territories: a pooled analysis of 2181 population-based studies with 65 million participants

Summary Background Comparable global data on health and nutrition of school-aged children and adolescents are scarce. We aimed to estimate age trajectories and time trends in mean height and mean body-mass index (BMI), which measures weight gain beyond what is expected from height gain, for school-aged children and adolescents. Methods For this pooled analysis, we used a database of cardiometabolic risk factors collated by the Non-Communicable Disease Risk Factor Collaboration. We applied a Bayesian hierarchical model to estimate trends from 1985 to 2019 in mean height and mean BMI in 1-year age groups for ages 5–19 years. The model allowed for non-linear changes over time in mean height and mean BMI and for non-linear changes with age of children and adolescents, including periods of rapid growth during adolescence. Findings We pooled data from 2181 population-based studies, with measurements of height and weight in 65 million participants in 200 countries and territories. In 2019, we estimated a difference of 20 cm or higher in mean height of 19-year-old adolescents between countries with the tallest populations (the Netherlands, Montenegro, Estonia, and Bosnia and Herzegovina for boys; and the Netherlands, Montenegro, Denmark, and Iceland for girls) and those with the shortest populations (Timor-Leste, Laos, Solomon Islands, and Papua New Guinea for boys; and Guatemala, Bangladesh, Nepal, and Timor-Leste for girls). In the same year, the difference between the highest mean BMI (in Pacific island countries, Kuwait, Bahrain, The Bahamas, Chile, the USA, and New Zealand for both boys and girls and in South Africa for girls) and lowest mean BMI (in India, Bangladesh, Timor-Leste, Ethiopia, and Chad for boys and girls; and in Japan and Romania for girls) was approximately 9–10 kg/m2. In some countries, children aged 5 years started with healthier height or BMI than the global median and, in some cases, as healthy as the best performing countries, but they became progressively less healthy compared with their comparators as they grew older by not growing as tall (eg, boys in Austria and Barbados, and girls in Belgium and Puerto Rico) or gaining too much weight for their height (eg, girls and boys in Kuwait, Bahrain, Fiji, Jamaica, and Mexico; and girls in South Africa and New Zealand). In other countries, growing children overtook the height of their comparators (eg, Latvia, Czech Republic, Morocco, and Iran) or curbed their weight gain (eg, Italy, France, and Croatia) in late childhood and adolescence. When changes in both height and BMI were considered, girls in South Korea, Vietnam, Saudi Arabia, Turkey, and some central Asian countries (eg, Armenia and Azerbaijan), and boys in central and western Europe (eg, Portugal, Denmark, Poland, and Montenegro) had the healthiest changes in anthropometric status over the past 3·5 decades because, compared with children and adolescents in other countries, they had a much larger gain in height than they did in BMI. The unhealthiest changes—gaining too little height, too much weight for their height compared with children in other countries, or both—occurred in many countries in sub-Saharan Africa, New Zealand, and the USA for boys and girls; in Malaysia and some Pacific island nations for boys; and in Mexico for girls. Interpretation The height and BMI trajectories over age and time of school-aged children and adolescents are highly variable across countries, which indicates heterogeneous nutritional quality and lifelong health advantages and risks

Cyclones of Subtropical Origin in the Southwest Pacific: a Climatology and Aspects of Movement and Development

A comprehensive study on cyclones of subtropical origin (STCs) in the Southwest Pacific is carried out. A brief history of the damage caused by STCs in New Zealand between 1990 and 2005 is given. It shows that approximately 2 to 3 times a year STCs come into the vicinity of New Zealand, mostly affecting the North Island and causing predominantly flood damage. A climatology is compiled with a cyclone track database covering 21 years, providing an overview of the behaviour and characteristics of STCs in this region. Distinct annual and seasonal patterns in frequency, tracks and intensity are revealed. Some of these patterns resemble those of tropical cyclones, in particular those undergoing extratropical transition, while others resemble those of extratropical cyclones in this region. In addition, it is shown that there is a significant increase in the number of summer STCs, which coincides with an increase in sea surface temperatures in the area. The structure and processes involved in the development of STCs are investigated in more detail using data from the United Kingdom Meteorological Office (UKMO) global model spanning 5 years (1999 to 2003). An analysis of the upper-level flow shows that STCs are steered into midlatitudes by upper-level baroclinic waves, m general through interaction with an upper-level trough. Differences in the structure and development of STCs can be attributed to the fact that upper-level baroclinic waves are able to propagate far into the sub tropics in this region. This is also the reason for the existence of three types of STCs, when differentiating by characteristics of their development process. Type 1 STCs are very similar to extratropical cyclones in structure and development. The structure and the development process of Type 3 STCs resemble more those of tropical cyclones. The initial development of Type 2 STCs is similar to that of Type 3, but they then undergo a transition, found to be very similar to that of tropical cyclones undergoing extratropical transition. Interseasonal variations in the upper-level flow over the Southwest Pacific are reflected in the behaviour and characteristics of STCs and subsequently the occurrence of the three types of STCs. During the colder seasons baroclinic waves frequently propagate relatively far into the subtropics in this region. This means STCs not only have a high chance of being picked up by an upper-level trough and undergoing extratropical transition, they are also able to actually form in the vicinity of a trough. Thus, during that time most STCs tend to be either Type 1 or 2. On the other hand, during summer, when baroclinic waves only occasionally propagate into the subtropics, there is a higher frequency of Type 3 STCs. In terms of weather-related threats to New Zealand, the interaction with an upperlevel trough is the cause for STCs coming into the vicinity of New Zealand, while the high rain rates that accompany them, and that are the cause for the extensive, mostly flood-related, damage, are attributed to their place of origin

Cyclones of Subtropical Origin in the Southwest Pacific: a Climatology and Aspects of Movement and Development

A comprehensive study on cyclones of subtropical origin (STCs) in the Southwest Pacific is carried out. A brief history of the damage caused by STCs in New Zealand between 1990 and 2005 is given. It shows that approximately 2 to 3 times a year STCs come into the vicinity of New Zealand, mostly affecting the North Island and causing predominantly flood damage. A climatology is compiled with a cyclone track database covering 21 years, providing an overview of the behaviour and characteristics of STCs in this region. Distinct annual and seasonal patterns in frequency, tracks and intensity are revealed. Some of these patterns resemble those of tropical cyclones, in particular those undergoing extratropical transition, while others resemble those of extratropical cyclones in this region. In addition, it is shown that there is a significant increase in the number of summer STCs, which coincides with an increase in sea surface temperatures in the area. The structure and processes involved in the development of STCs are investigated in more detail using data from the United Kingdom Meteorological Office (UKMO) global model spanning 5 years (1999 to 2003). An analysis of the upper-level flow shows that STCs are steered into midlatitudes by upper-level baroclinic waves, m general through interaction with an upper-level trough. Differences in the structure and development of STCs can be attributed to the fact that upper-level baroclinic waves are able to propagate far into the sub tropics in this region. This is also the reason for the existence of three types of STCs, when differentiating by characteristics of their development process. Type 1 STCs are very similar to extratropical cyclones in structure and development. The structure and the development process of Type 3 STCs resemble more those of tropical cyclones. The initial development of Type 2 STCs is similar to that of Type 3, but they then undergo a transition, found to be very similar to that of tropical cyclones undergoing extratropical transition. Interseasonal variations in the upper-level flow over the Southwest Pacific are reflected in the behaviour and characteristics of STCs and subsequently the occurrence of the three types of STCs. During the colder seasons baroclinic waves frequently propagate relatively far into the subtropics in this region. This means STCs not only have a high chance of being picked up by an upper-level trough and undergoing extratropical transition, they are also able to actually form in the vicinity of a trough. Thus, during that time most STCs tend to be either Type 1 or 2. On the other hand, during summer, when baroclinic waves only occasionally propagate into the subtropics, there is a higher frequency of Type 3 STCs. In terms of weather-related threats to New Zealand, the interaction with an upperlevel trough is the cause for STCs coming into the vicinity of New Zealand, while the high rain rates that accompany them, and that are the cause for the extensive, mostly flood-related, damage, are attributed to their place of origin.</p

“Zebrafishing” for Novel Genes Relevant to the Glomerular Filtration Barrier

Data for genes relevant to glomerular filtration barrier function or proteinuria is continually increasing in an era of microarrays, genome-wide association studies, and quantitative trait locus analysis. Researchers are limited by published literature searches to select the most relevant genes to investigate. High-throughput cell cultures and other in vitro systems ultimately need to demonstrate proof in an in vivo model. Generating mammalian models for the genes of interest is costly and time intensive, and yields only a small number of test subjects. These models also have many pitfalls such as possible embryonic mortality and failure to generate phenotypes or generate nonkidney specific phenotypes. Here we describe an in vivo zebrafish model as a simple vertebrate screening system to identify genes relevant to glomerular filtration barrier function. Using our technology, we are able to screen entirely novel genes in 4–6 weeks in hundreds of live test subjects at a fraction of the cost of a mammalian model. Our system produces consistent and reliable evidence for gene relevance in glomerular kidney disease; the results then provide merit for further analysis in mammalian models