Optimization of cellulase and &#223-glucosidase induction by sugarbeet pathogen Sclerotium rolfsii

The ability to produce cellulose degrading enzymes by sugarbeet pathogen Sclerotium rolfsii Sacc. in liquid synthetic media with carboxymethy cellulose (CMC) as inducer was studied. Several culturalconditions were examined to assess their effect in optimizing enzymes production. Shaking cultures gave higher yields of cellulases compared with static ones. Aspargine supplement was the best nitrogen source, especially at 3.0 g/l concentration, in promoting enzyme production. Variation of cellulose/xylan ratio in the culture medium showed that cellulose and xylan induced both cellulases synthesis but cellulose being the most effective specific substrate. The influence of different inhibitorson enzymes production by S. rolfsii was also studied. Cyclohexmide and ethidium bromide inhibited protein synthesis by S. rolfsii. Moreover, glucose repressed cellulase synthesis in S. rolfsii

Dynamique Hydro-Erosive Actuelle Des Bassins Versants Endoreiques De La Region De Niamey (Sud-Ouest Du Niger)

La généralisation du ravinement et la baisse de la productivité des terres sont quelques-unes des principales contraintes qui assaillent l’utilisation des sols au Sahel. Pour gérer efficacement ces sols, une évaluation des processus hydro-érosifs est nécessaire. Ce travail a ainsi pour objectif d’analyser la dynamique hydro-érosive sur un site expérimental installé depuis 2004 dans la région de Niamey (Niger). Sur ce site, le dispositif de mesure est composé de huit parcelles de mesures de ruissellement et d’érosion, des stations hydrométriques à l’exutoire des deux bassins versants endoréiques et de plusieurs piquets destinés aux suivis morpho-dynamiques des ravines. Après une décennie d’observation, les ruissellements mesurés sur les parcelles se caractérisent par une dynamique saisonnière croissante sur les surfaces encroûtées (croûtes biologique et d’érosion) et décroissante sur les surfaces cultivées. Le coefficient de ruissellement varie de 5 % sur la surface cultivée à 58 % sur la croûte d’érosion. Au cours des 10 années de mesure, ce coefficient a connu une forte croissance en particulier sur la jachère (+ 80 %) et sur la surface cultivée (+ 300 %), traduisant ainsi la dégradation des sols. A l’échelle des bassins versants, l’augmentation du coefficient de ruissellement s’accompagne d’une érosion aréolaire qui décape le sol à une vitesse moyenne de 5 mm/an et d’une érosion linéaire active, de l’ordre de 4 m3/an au niveau des ravines suivies. Les transferts sédimentaires qui en résultent agissent sur le fonctionnement morpho-sédimentaire des cours d’eau. Des aménagements antiérosifs sont nécessaires pour dissiper les processus hydro-érosifs et préserver les services écosystémiques des sols des bassins. Widespread gullying and the declining land productivity are some of the main constraints plaguing land use in the Sahel. In order to effectively manage these soils, it has become necessary to assess the hydro-erosive processes. The current study, thus, aims at analyzing the hydro-erosion dynamics on an experimental site installed since 2004 in the Niamey region (Niger Republic). On this site, the measurement device is made up of eight runoff and erosion measurement plots, hydrometric stations at the outlet of the two endorheic watersheds and several stakes intended for morpho-dynamic monitoring of the gullies. After a decade of observation, the runoff measured on the plots is characterized by an increasing seasonal dynamic on encrusted surfaces (biological and erosion crusts) and a decreasing one on cultivated surfaces. The runoff coefficient varies from 5% on the cultivated area to 58% on the erosion crust. Over the 10 years of measurement, this coefficient has greatly increased, especially on the fallow (+ 80%) and on cultivated area (+ 300%); this increase consequently reflects soil degradation. At the watershed scale, the increase in the runoff coefficient is accompanied by area erosion (or the erosion of the area out of the basin) which strikes the soil at an average speed of 5 mm/year and active linear erosion measuring 4 m3/year as observed at the monitored (the ongoing investigated) gullies. The resulting sediment transfers act on the morpho-sedimentary functioning of rivers. Anti-erosion facilities are necessary to dissipate the hydro-erosive processes and preserve the ecosystem services of the soil in the basin

Multi-messenger observations of a binary neutron star merger

On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ~1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40+8-8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 Mo. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ~40 Mpc) less than 11 hours after the merger by the One- Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ~10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position ~9 and ~16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta

Combination of searches for heavy spin-1 resonances using 139 fb−1 of proton-proton collision data at s = 13 TeV with the ATLAS detector

A combination of searches for new heavy spin-1 resonances decaying into different pairings of W, Z, or Higgs bosons, as well as directly into leptons or quarks, is presented. The data sample used corresponds to 139 fb−1 of proton-proton collisions at = 13 TeV collected during 2015–2018 with the ATLAS detector at the CERN Large Hadron Collider. Analyses selecting quark pairs (qq, bb, , and tb) or third-generation leptons (τν and ττ) are included in this kind of combination for the first time. A simplified model predicting a spin-1 heavy vector-boson triplet is used. Cross-section limits are set at the 95% confidence level and are compared with predictions for the benchmark model. These limits are also expressed in terms of constraints on couplings of the heavy vector-boson triplet to quarks, leptons, and the Higgs boson. The complementarity of the various analyses increases the sensitivity to new physics, and the resulting constraints are stronger than those from any individual analysis considered. The data exclude a heavy vector-boson triplet with mass below 5.8 TeV in a weakly coupled scenario, below 4.4 TeV in a strongly coupled scenario, and up to 1.5 TeV in the case of production via vector-boson fusion

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Resveratrol regulates neuro-inflammation and induces adaptive immunity in Alzheimer’s disease

BACKGROUND: Treatment of mild-moderate Alzheimer’s disease (AD) subjects (N = 119) for 52 weeks with the SIRT1 activator resveratrol (up to 1 g by mouth twice daily) attenuates progressive declines in CSF Aβ40 levels and activities of daily living (ADL) scores. METHODS: For this retrospective study, we examined banked CSF and plasma samples from a subset of AD subjects with CSF Aβ42 <600 ng/ml (biomarker-confirmed AD) at baseline (N = 19 resveratrol-treated and N = 19 placebo-treated). We utilized multiplex Xmap technology to measure markers of neurodegenerative disease and metalloproteinases (MMPs) in parallel in CSF and plasma samples. RESULTS: Compared to the placebo-treated group, at 52 weeks, resveratrol markedly reduced CSF MMP9 and increased macrophage-derived chemokine (MDC), interleukin (IL)-4, and fibroblast growth factor (FGF)-2. Compared to baseline, resveratrol increased plasma MMP10 and decreased IL-12P40, IL12P70, and RANTES. In this subset analysis, resveratrol treatment attenuated declines in mini-mental status examination (MMSE) scores, change in ADL (ADCS-ADL) scores, and CSF Aβ42 levels during the 52-week trial, but did not alter tau levels. CONCLUSIONS: Collectively, these data suggest that resveratrol decreases CSF MMP9, modulates neuro-inflammation, and induces adaptive immunity. SIRT1 activation may be a viable target for treatment or prevention of neurodegenerative disorders. TRIAL REGISTRATION: ClinicalTrials.gov NCT0150485

Comparative metagenomics approaches to characterize the soil fungal communities of western coastal region, Saudi Arabia

A total of 145007 reads were obtained from pyrosequencing for all the 4 samples. The total count ranged from 11,301,014 (Mecca old road) to 23,503,512 bp (Thuwal). A total of 460 fungal species belonging to 133 genera, 58 families, 33 orders, 13 classes and 4 phyla was identified across the four sites. The most abundant phylum at all four sites was Ascomycota followed by Basidiomycota. Four phyla (Ascomycota-99.31%, Basidiomycota-0.59%, Chytridiomycota-0.04%, Glomeromycota-0.03%) were detected in Khulais. Except for Glomeromycota, all phyla were detected at Mecca old road (Ascomycota-74.26%, Basidiomycota-25.71%, Chytridiomycota-0.01%) and Thuwal (Ascomycota-99.59%, Basidiomycota-0.40%, Chytridiomycota-0.002%); while only Ascomycota-90.98% and Basidiomycota-9.01% were detected in Asfan road. At the class level, Sordariomycetes was predominantly observed at Asfan road-59.88%, Khulais-68.26% and Thuwal-94.84%; while Pezizomycetes was dominant at Mecca old road-56.01%, was absent at Asfan road. Agaricomycetes was present only at Mecca old road-25.73%; while Tremellomycetes-5.77%, Malasseizomycetes-2.13% and Microbotryomycetes-1.10% were found only at Asfan road. The phylogenetic trees revealed that clear genus level differences are visible across all the four sites, with an overall predominance of Thielavia followed by Madurella, Aspergillus, and Gelasinospora. Chaetomium sp., Aspergillus caespitosus and Aspergillus sp. were found in moderate (Mecca old road and Thuwal) to abundant (Asfan road and Khulais) quantities. Thielavia sp., Thielavia hyalocarpa and Madurella sp. are found in moderate quantities at Khulais and Mecca old road, while in abundant levels at Asfan road and Thuwal. Fusarium equisati and F. oxysporum were detected at Thuwal and Khulais. Sordaria araneosa was present at Khulais, while Malasseiza globosa species was detected in moderate quantities across all sites except Khulais