Sedimentary processes in the Thau Lagoon (France): From seasonal to century time scales

As a part of the MICROBENT programme, an investigation of the sedimentation framework was carried out at the water-sediment interface in the Thau Lagoon (French Mediterranean coast). Two main sites, C4 in the middle of the lagoon and C5 near oyster farms, were visited six times between December 2001 and May 2003. Interface sediments were studied using classical sedimentology parameters (radiography RX, grain size distribution) and analysis of selected radionuclides (234Th, 7Be, 210Pb, 226Ra). On a century time scale, excess 210Pb (210Pbxs) presents classical profiles with an upper mixed layer, followed by an exponential decrease of activities to undetectable levels below 20 – 30 cm. At the central site, C4, cores seem to register episodic changes in mean grain size, presenting recurrently peaks. The upper 10 cm of 210Pbxs profiles at site C5 exhibit a mixed layer associated with coarser sediments: this could be related to biological activity. Sedimentation rates derived from 210Pbxs varied from 0.15 cm y−1 at the edge of the basin, to 0.25 cm y−1 at the central site. On a seasonal time scale, 234Th and 7Be both show significant variations in activities and in penetration within the sediment. Bioturbation rates derived from both radionuclides agree well and range between 1–10 cm2 y−1 at site C4 and 1–31 cm2 y−1 at site C5. 234Th and 7Be fluxes at the water-sediment interface show too seasonal variations, more pronounced for site C5. This latter site presents especially a higher variability that is well marked with season, probably in relation with its position near oyster farms

Transport property study of MgO-GaAs(001) contacts for spin injection devices

International audienceThe electrical properties of Au/MgO/n-GaAs(001) tunnel structures have been investigated with capacitance-voltage and current-voltage measurements at room temperature with various MgO thicknesses between 0.5 and 6.0nm. For an oxide thickness higher than 2nm and for low bias voltages, the voltage essentially drops across the oxide and the structure progressively enters the high-current mode of operation with increasing reverse bias voltage, the property sought in spin injection devices. In this mode, we demonstrate that a large amount of charge accumulates at the MgO/GaAsinterface in interface traps located in the semiconductor band gap

From Research to Operational Biomonitoring of Freshwaters: A Suggested Conceptual Framework and Practical Solutions

International audienceThe contradictory demands of managers (quick relevant operational responses) and ecologists (need time for in-depth research) involved in freshwater biomonitoring are still relevant today. To contribute to solving this dilemma, we are proposing a novel biomonitoring approach, which among many others, could be used in this field and further developed in the future. Biomonitoring actions are integrated in conceptual schemes, in which hydrology, chemistry, hydrogeology and geomorphology bear as much importance as biology. Among biomonitoring tools, a harmonization system allows end-users to use a set of qualitative indicators (various biotic indices) and integrate the information given by individual biotic indices. Functional traits and calculation of an ecological potential in porous aquatic habitats (surficial coarse sediments and the hyporheic system) are regarded as a basis for assessing ecological functioning of streams and rivers. This last methodology takes into account the dynamics of water exchanges between surface water and groundwater. Objectives of ecological quality, ecological potentials and resilience capacity that need to be preserved or rehabilitated in aquatic habitats are established. In lakes, a similar approach was followed and a general typology of lake functioning was proposed, including that for urban lakes. All those biomonitoring tools are transferred to end-users and subject to further research. The final purpose is to promote practical high-tech tools which are continually and interactively connected with ongoing research

Digital expression profiling of novel diatom transcripts provides insight into their biological functions

Background: Diatoms represent the predominant group of eukaryotic phytoplankton in the oceans and are responsible for around 20% of global photosynthesis. Two whole genome sequences are now available. Notwithstanding, our knowledge of diatom biology remains limited because only around half of their genes can be ascribed a function based onhomology-based methods. High throughput tools are needed, therefore, to associate functions with diatom-specific genes. Results: We have performed a systematic analysis of 130,000 ESTs derived from Phaeodactylum tricornutum cells grown in 16 different conditions. These include different sources of nitrogen, different concentrations of carbon dioxide, silicate and iron, and abiotic stresses such as low temperature and low salinity. Based on unbiased statistical methods, we have catalogued transcripts with similar expression profiles and identified transcripts differentially expressed in response to specific treatments. Functional annotation of these transcripts provides insights into expression patterns of genes involved in various metabolic and regulatory pathways and into the roles of novel genes with unknown functions. Specific growth conditions could be associated with enhanced gene diversity, known gene product functions, and over-representation of novel transcripts. Comparative analysis of data from the other sequenced diatom, Thalassiosira pseudonana, helped identify several unique diatom genes that are specifically regulated under particular conditions, thus facilitating studies of gene function, genome annotation and the molecular basis of species diversity. Conclusions: The digital gene expression database represents a new resource for identifying candidate diatom-specific genes involved in processes of major ecological relevance

Genome-Wide Transcriptome Analyses of Silicon Metabolism in Phaeodactylum tricornutum Reveal the Multilevel Regulation of Silicic Acid Transporters

BACKGROUND:Diatoms are largely responsible for production of biogenic silica in the global ocean. However, in surface seawater, Si(OH)(4) can be a major limiting factor for diatom productivity. Analyzing at the global scale the genes networks involved in Si transport and metabolism is critical in order to elucidate Si biomineralization, and to understand diatoms contribution to biogeochemical cycles. METHODOLOGY/PRINCIPAL FINDINGS:Using whole genome expression analyses we evaluated the transcriptional response to Si availability for the model species Phaeodactylum tricornutum. Among the differentially regulated genes we found genes involved in glutamine-nitrogen pathways, encoding putative extracellular matrix components, or involved in iron regulation. Some of these compounds may be good candidates for intracellular intermediates involved in silicic acid storage and/or intracellular transport, which are very important processes that remain mysterious in diatoms. Expression analyses and localization studies gave the first picture of the spatial distribution of a silicic acid transporter in a diatom model species, and support the existence of transcriptional and post-transcriptional regulations. CONCLUSIONS/SIGNIFICANCE:Our global analyses revealed that about one fourth of the differentially expressed genes are organized in clusters, underlying a possible evolution of P. tricornutum genome, and perhaps other pennate diatoms, toward a better optimization of its response to variable environmental stimuli. High fitness and adaptation of diatoms to various Si levels in marine environments might arise in part by global regulations from gene (expression level) to genomic (organization in clusters, dosage compensation by gene duplication), and by post-transcriptional regulation and spatial distribution of SIT proteins

Performance of the ATLAS Electromagnetic Calorimeter End-cap Module 0

The construction and beam test results of the ATLAS electromagnetic end-cap calorimeter pre-production module 0 are presented. The stochastic term of the energy resolution is between 10% GeV^1/2 and 12.5% GeV^1/2 over the full pseudorapidity range. Position and angular resolutions are found to be in agreement with simulation. A global constant term of 0.6% is obtained in the pseudorapidity range 2.5 < eta < 3.2 (inner wheel)

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements