35 research outputs found
SKYSURF-4: Panchromatic HST All-Sky Surface-Brightness Measurement Methods and Results
The diffuse, unresolved sky provides most of the photons that the Hubble
Space Telescope (HST) receives, yet remains poorly understood. HST Archival
Legacy program SKYSURF aims to measure the 0.2-1.6 m sky surface
brightness (sky-SB) from over 140,000 HST images. We describe a sky-SB
measurement algorithm designed for SKYSURF that is able to recover the input
sky-SB from simulated images to within 1% uncertainty. We present our sky-SB
measurements estimated using this algorithm on the entire SKYSURF database.
Comparing our sky-SB spectral energy distribution (SED) to measurements from
the literature shows general agreements. Our SKYSURF SED also reveals a
possible dependence on Sun angle, indicating either non-isotropic scattering of
solar photons off interplanetary dust or an additional component to Zodiacal
Light. Finally, we update Diffuse Light limits in the near-IR based on the
methods from Carleton et al. (2022), with values of 0.009 MJy sr (22 nW
m sr) at 1.25 m, 0.015 MJy sr (32 nW m
sr) at 1.4 m, and 0.013 MJy sr (25 nW m sr) at
1.6 m. These estimates provide the most stringent all-sky constraints to
date in this wavelength range. SKYSURF sky-SB measurements are made public on
the official SKYSURF website and will be used to constrain Diffuse Light in
future papers.Comment: Revised based on helpful comments from the reviewer, and accepted to
AJ on April 12th, 2023. Main paper: 18 pages, 9 figures, 4 tables.
Appendices: 16 pages, 10 figures, 1 table. Main results shown in Figure 7 and
Table
SKYSURF: Constraints on Zodiacal Light and Extragalactic Background Light through Panchromatic HST All-Sky Surface-Brightness Measurements: I. Survey Overview and Methods
We give an overview and describe the rationale, methods, and testing of the
Hubble Space Telescope (HST) Archival Legacy project "SKYSURF." SKYSURF uses
HST's unique capability as an absolute photometer to measure the ~0.2-1.7
m sky surface brightness (SB) from 249,861 WFPC2, ACS, and WFC3 exposures
in ~1400 independent HST fields. SKYSURF's panchromatic dataset is designed to
constrain the discrete and diffuse UV to near-IR sky components: Zodiacal Light
(ZL; inner Solar System), Kuiper Belt Objects (KBOs; outer Solar System),
Diffuse Galactic Light (DGL), and the discrete plus diffuse Extragalactic
Background Light (EBL). We outline SKYSURF's methods to: (1) measure sky-SB
levels between its detected objects; (2) measure the integrated discrete EBL,
most of which comes from AB17-22 mag galaxies; and (3) estimate how
much diffuse light may exist in addition to the extrapolated discrete galaxy
counts. Simulations of HST WFC3/IR images with known sky-values and gradients,
realistic cosmic ray (CR) distributions, and star plus galaxy counts were
processed with nine different algorithms to measure the "Lowest Estimated
Sky-SB" (LES) in each image between the discrete objects. The best algorithms
recover the inserted LES values within 0.2% when there are no image gradients,
and within 0.2-0.4% when there are 5-10% gradients. SKYSURF requires
non-standard re-processing of these HST images that includes restoring the
lowest sky-level from each visit into each drizzled image. We provide a proof
of concept of our methods from the WFC3/IR F125W images, where any residual
diffuse light that HST sees in excess of the Kelsall et al. (1998) Zodiacal
model prediction does not depend on the total object flux that each image
contains. This enables us to present our first SKYSURF results on diffuse light
in Carleton et al. (2022).Comment: Accepted to AJ; see accompanying paper Carleton et al. 2022:
arXiv:2205.06347. Comments welcome
Common genetic determinants of intraocular pressure and primary open-angle Glaucoma
10.1371/journal.pgen.1002611PLoS Genetics85
The James Webb Space Telescope
The James Webb Space Telescope (JWST) is a large (6.6m), cold (50K),
infrared-optimized space observatory that will be launched early in the next
decade. The observatory will have four instruments: a near-infrared camera, a
near-infrared multi-object spectrograph, and a tunable filter imager will cover
the wavelength range, 0.6 to 5.0 microns, while the mid-infrared instrument
will do both imaging and spectroscopy from 5.0 to 29 microns. The JWST science
goals are divided into four themes. The End of the Dark Ages: First Light and
Reionization theme seeks to identify the first luminous sources to form and to
determine the ionization history of the early universe. The Assembly of
Galaxies theme seeks to determine how galaxies and the dark matter, gas, stars,
metals, morphological structures, and active nuclei within them evolved from
the epoch of reionization to the present day. The Birth of Stars and
Protoplanetary Systems theme seeks to unravel the birth and early evolution of
stars, from infall on to dust-enshrouded protostars to the genesis of planetary
systems. The Planetary Systems and the Origins of Life theme seeks to determine
the physical and chemical properties of planetary systems including our own,
and investigate the potential for the origins of life in those systems. To
enable these observations, JWST consists of a telescope, an instrument package,
a spacecraft and a sunshield. The telescope consists of 18 beryllium segments,
some of which are deployed. The segments will be brought into optical alignment
on-orbit through a process of periodic wavefront sensing and control. The JWST
operations plan is based on that used for previous space observatories, and the
majority of JWST observing time will be allocated to the international
astronomical community through annual peer-reviewed proposal opportunities.Comment: 96 pages, including 48 figures and 15 tables, accepted by Space
Science Review
Large expert-curated database for benchmarking document similarity detection in biomedical literature search
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe
Targeting EXT1 reveals a crucial role for heparan sulfate in the growth of multiple myeloma
Expression of the heparan sulfate proteoglycan syndecan-1 is a hallmark of both normal and multiple myeloma (MM) plasma cells. Syndecan-1 could affect plasma cell fate by strengthening integrinmediated adhesion via its core protein and/or by accommodating and presenting soluble factors via its HS side chains. Here, we show that inducible RNAi-mediated knockdown of syndecan-1 in human MM cells leads to reduced growth rates and a strong increase of apoptosis. Importantly, knockdown of EXT1, a copolymerase critical for HS chain biosynthesis, had similar effects. Using an innovative myeloma xenotransplantation model in Rag-2(-/-)gamma(-/-)(c) mice, we demonstrate that induction of EXT1 knockdown in vivo dramatically suppresses the growth of bone marrow localized myeloma. Our findings provide direct evidence that the HS chains of syndecan-1 are crucial for the growth and survival of MM cells within the bone marrow environment, and indicate the HS biosynthesis machinery as a potential treatment target in MM. (Blood. 2010; 115: 601-604
N-cadherin-mediated interaction with multiple myeloma cells inhibits osteoblast differentiation
Background Multiple myeloma is a hematologic malignancy characterized by a clonal expansion of malignant plasma cells in the bone marrow, which is accompanied by the development of osteolytic lesions and/or diffuse osteopenia. The intricate bi-directional interaction with the bone marrow microenvironment plays a critical role in sustaining the growth and survival of myeloma cells during tumor progression. Identification and functional analysis of the (adhesion) molecules involved in this interaction will provide important insights into the pathogenesis of multiple myeloma. Design and Methods Multiple myeloma cell lines and patients' samples were analyzed for expression of the adhesion molecule N-cadherin by immunoblotting, flow cytometry, immunofluorescence microscopy, immunohistochemistry and expression microarray. In addition, by means of blocking antibodies and inducible RNA interference we studied the functional consequence of N-cadherin expression for the myeloma cells, by analysis of adhesion, migration and growth, and for the bone marrow microenvironment, by analysis of osteogenic differentiation. Results The malignant plasma cells in approximately half of the multiple myeloma patients, belonging to specific genetic subgroups, aberrantly expressed the homophilic adhesion molecule N-cadherin. N-cadherin-mediated cell-substrate or homotypic cell-cell adhesion did not contribute to myeloma cell growth in vitro. However, N-cadherin directly mediated the bone marrow localization/retention of myeloma cells in vivo, and facilitated a close interaction between myeloma cells and N-cadherin-positive osteoblasts. Furthermore, this N-cadherin-mediated interaction contributed to the ability of myeloma cells to inhibit osteoblastogenesis. Conclusions Taken together, our data show that myeloma cells frequently display aberrant expression of N-cadherin and that N-cadherin mediates the interaction of myeloma cells with the bone marrow microenvironment, in particular the osteoblasts. This N-cadherin-mediated interaction inhibits osteoblast differentiation and may play an important role in the pathogenesis of myeloma bone diseas
Antibody responses to SARS-CoV-2 mrna vaccines are detectable in Saliva
The approved Pfizer and Moderna mRNA vaccines are well known to induce serum antibody responses to the SARS-CoV-2 Spike (S)-protein. However, their abilities to elicit mucosal immune responses have not been reported. Saliva antibodies represent mucosal responses that may be relevant to how mRNA vaccines prevent oral and nasal SARS-CoV-2 transmission. Here, we describe the outcome of a cross-sectional study on a healthcare worker cohort (WEL-COME-NYPH), in which we assessed whether IgM, IgG, and IgA antibodies to the S-protein and its receptor-binding domain (RBD) were present in serum and saliva samples. Anti-S-protein IgG was detected in 14/31 and 66/66 of saliva samples from uninfected participants after vaccine dos-es-1 and-2, respectively. IgA antibodies to the S-protein were present in 40/66 saliva samples after dose 2. Anti-S-protein IgG was present in every serum sample from recipients of 2 vaccine doses. Vaccine-induced antibodies against the RBD were also frequently present in saliva and sera. These findings may help our understanding of whether and how vaccines may impede SARS-CoV-2 transmission, including to oral cavity target cells