48 research outputs found
Characterization of the human immunodeficiency virus type 1 enhancer-binding proteins from the human T-cell line Jurkat
The transcription of the human immunodeficiency virus type 1 (HIV-1) is under the control of cellular proteins that bind to the viral long terminal repeat (LTR). Among the protein-binding regions of the HIV-1 LTR is the transcription-enhancer region. We show that at least one inducible, C1, and one constitutive, C2, protein can bind to the HIV enhancer in Jurkat cells. The two proteins differ in their surface charge, since they are separable by anion-exchange chromatography. Bivalent cations such as Mg2+ and Zn2+ differentially affect their binding to oligonucleotides which contain the HIV-enhancer domain. Both C1 and C2 proteins also bind to a similar sequence found in the interleukin-2-receptor alpha-subunit enhancer. The inducible C1 protein was partially purified by three chromatographic steps and characterized by u.v. cross-linking as a 47 kDa protein
Cosmic microwave background and large scale structure limits on the interaction between dark matter and baryons
We study the effect on the cosmic microwave background (CMB) anisotropy and
large scale structure (LSS) power spectrum of a scattering interaction between
cold dark matter and baryons. This scattering alters the CMB anisotropy and LSS
spectrum through momentum transfer between the cold dark matter particles and
the baryons. We find that current CMB observations can put an upper limit on
the scattering cross section which is comparable with or slightly stronger than
previous disk heating constraints at masses greater than 1 GeV, and much
stronger at smaller masses. When large-scale structure constraints are added to
the CMB limits, our constraint is more stringent than this previous limit at
all masses. In particular, a dark matter-baryon scattering cross section
comparable to the ``Spergel-Steinhardt'' cross section is ruled out for dark
matter mass greater than 1 GeV.Comment: 8 pages, 2 figures, use RevTeX4, submitted to PRD replaced with
revised versio
One-Loop Helicity Amplitudes for Parton Level Virtual Compton Scattering
We calculate the one-loop QCD virtual corrections to all helicity amplitudes
for parton level virtual Compton scattering processes. We include the
amplitudes both on quark target process and on gluon
target process . The infrared pole structure of the
amplitudes is in agreement with the prediction of Catani's general formalism
for the singularities of one-loop amplitudes, while expressions for the finite
remainder are given in terms of logarithms and dilogarithms that are real in
the physical region.Comment: 16 pages, 2 figures, detailed comparison with DVCS include
Cosmological model with interactions in the dark sector
A cosmological model is proposed for the current Universe consisted of
non-interacting baryonic matter and interacting dark components. The dark
energy and dark matter are coupled through their effective barotropic indexes,
which are considered as functions of the ratio between their energy densities.
It is investigated two cases where the ratio is asymptotically stable and their
parameters are adjusted by considering best fits to Hubble function data. It is
shown that the deceleration parameter, the densities parameters, and the
luminosity distance have the correct behavior which is expected for a viable
present scenario of the Universe.Comment: 6 pages, 8 figure
Coupled dark matter-dark energy in light of near Universe observations
Cosmological analysis based on currently available observations are unable to
rule out a sizeable coupling among the dark energy and dark matter fluids. We
explore a variety of coupled dark matter-dark energy models, which satisfy
cosmic microwave background constraints, in light of low redshift and near
universe observations. We illustrate the phenomenology of different classes of
dark coupling models, paying particular attention in distinguishing between
effects that appear only on the expansion history and those that appear in the
growth of structure. We find that while a broad class of dark coupling models
are effectively models where general relativity (GR) is modified --and thus can
be probed by a combination of tests for the expansion history and the growth of
structure--, there is a class of dark coupling models where gravity is still
GR, but the growth of perturbations is, in principle modified. While this
effect is small in the specific models we have considered, one should bear in
mind that an inconsistency between reconstructed expansion history and growth
may not uniquely indicate deviations from GR. Our low redshift constraints
arise from cosmic velocities, redshift space distortions and dark matter
abundance in galaxy voids. We find that current data constrain the
dimensionless coupling to be |xi|<0.2, but prospects from forthcoming data are
for a significant improvement. Future, precise measurements of the Hubble
constant, combined with high-precision constraints on the growth of structure,
could provide the key to rule out dark coupling models which survive other
tests. We shall exploit as well weak equivalence principle violation arguments,
which have the potential to highly disfavour a broad family of coupled models.Comment: 34 pages, 6 figures; changes to match published versio
Search for Supersymmetry in Di-Photon Final States at sqrt{s} = 1.96 TeV
We report results of a search for supersymmetry (SUSY) with gauge-mediated
symmetry breaking in di-photon events collected by the D0 experiment at the
Fermilab Tevatron Collider in 2002--2006. In 1.1 fb of data, we find no
significant excess beyond the background expected from the standard model and
set the most stringent lower limits to date for a standard benchmark model on
the lightest neutralino and chargino masses of 125 GeV and 229 GeV,
respectively, at 95% confidence
IFN-gamma inhibits c-myc gene expression by impairing the splicing process in a colony-stimulating factor dependent murine myeloid cell line
Proliferation of NSF-60.8 cells, a CSF-dependent murine myeloid cell line, is strongly inhibited by murine IFN-gamma. Northern analysis of growth arrested, NSF-60.8 cells activated by addition of CSF in the presence or absence of IFN-gamma indicated that IFN-gamma inhibited the induction of c-myc mRNA steady state level by CSF. The effect was observed as early as 30 min after induction and the inhibition was complete after 20 h. IFN-gamma did not impair the transcriptional activation of c-myc gene, and it had only a slight destabilizing effect on the mature c-myc message. Study of the processing steps of c-myc mRNA precursor indicated that in the presence of IFN-gamma, a putative 3.6-kb splice intermediate accumulated instead of the mature message, suggesting that IFN-gamma inhibits the splicing of c-myc precursor