[INVITED] Novel optical biosensing technologies for detection of mycotoxins

This work reviews our recent progress in development of novel optical methods of detection of mycotoxins in direct assay with either specific antibodies or aptamers. The main method in this work was the total internal reflection ellipsometry (TIRE) combined with LSPR transducers based on gold nano-structures produced by annealing of thin gold films. The gold nano-islands produced were characterised with SEM, AFM, UV–visible absorption spectroscopy, and spectroscopic ellipsometry. The combination of TIRE and LSPR offers superior refractive index sensitivity as compared to traditional UV–vis absorption spectroscopy. The limitations of LSPR related to a short evanescent field decay length can be overcome using small-size bio-receptors, such as half-antibodies and aptamers. The achieved sensitivity of detection of mycotoxins in 0.01 ppb level of concentration is sufficient for the use of this method for analysis of agriculture products, food and feed on the presence of mycotoxins. Even higher sensitivity in sub-ppt level was achieved with another optical biosensor developed recently; it is based on optical planar waveguide operating as polarization interferometer (PI). This method is promising for development of portable, highly sensitive, and simple to use biosensors suitable for point-of-need detection of mycotoxins

Liposomes in Biology and Medicine

Drug delivery systems (DDS) have become important tools for the specific delivery of a large number of drug molecules. Since their discovery in the 1960s liposomes were recognized as models to study biological membranes and as versatile DDS of both hydrophilic and lipophilic molecules. Liposomes--nanosized unilamellar phospholipid bilayer vesicles--undoubtedly represent the most extensively studied and advanced drug delivery vehicles. After a long period of research and development efforts, liposome-formulated drugs have now entered the clinics to treat cancer and systemic or local fungal infections, mainly because they are biologically inert and biocompatible and practically do not cause unwanted toxic or antigenic reactions. A novel, up-coming and promising therapy approach for the treatment of solid tumors is the depletion of macrophages, particularly tumor associated macrophages with bisphosphonate-containing liposomes. In the advent of the use of genetic material as therapeutic molecules the development of delivery systems to target such novel drug molecules to cells or to target organs becomes increasingly important. Liposomes, in particular lipid-DNA complexes termed lipoplexes, compete successfully with viral gene transfection systems in this field of application. Future DDS will mostly be based on protein, peptide and DNA therapeutics and their next generation analogs and derivatives. Due to their versatility and vast body of known properties liposome-based formulations will continue to occupy a leading role among the large selection of emerging DDS

Evaluation of sesamum gum as an excipient in matrix tablets

In developing countries modern medicines are often beyond the affordability of the majority of the population. This is due to the reliance on expensive imported raw materials despite the abundance of natural resources which could provide an equivalent or even an improved function. The aim of this study was to investigate the potential of sesamum gum (SG) extracted from the leaves of Sesamum radiatum (readily cultivated in sub-Saharan Africa) as a matrix former. Directly compressed matrix tablets were prepared from the extract and compared with similar matrices of HPMC (K4M) using theophylline as a model water soluble drug. The compaction, swelling, erosion and drug release from the matrices were studied in deionized water, 0.1 N HCl (pH 1.2) and phosphate buffer (pH 6.8) using USP apparatus II. The data from the swelling, erosion and drug release studies were also fitted into the respective mathematical models. Results showed that the matrices underwent a combination of swelling and erosion, with the swelling action being controlled by the rate of hydration in the medium. SG also controlled the release of theophylline similar to the HPMC and therefore may have use as an alternative excipient in regions where Sesamum radiatum can be easily cultivated

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe

Raman spectroscopy of free-standing individual semiconducting single-wall carbon nanotubes

International audienceThe radial breathing modes and tangential modes have been systematically measured on a large number of individual semiconducting single-wall carbon nanotubes (thin bundles) suspended between plots (free-standing single-wall carbon nanotubes). The strong intensity of the Raman spectra ensures the precision of the experimentally determined line shapes and frequencies of these modes. The diameter dependence of the frequencies of the tangential modes was measured. This dependence is discussed in relation with recent calculations. The present data confirm/contradict some previous interpretations

Last glacial maximum cooling of 9 °C in continental Europe from a 40 kyr-long noble gas paleothermometry record

International audienceThe Last Glacial Maximum (LGM; ∼26-18 kyr ago) is a time interval of great climatic interest characterized by substantial global cooling driven by radiative forcings and feedbacks associated with orbital changes, lower atmospheric CO2, and large ice sheets. However, reliable proxies of continental paleotemperatures are scarce and often qualitative, which has limited our understanding of the spatial structure of past climate changes. Here, we present a quantitative noble gas temperature (NGT) record of the last ∼40 kyr from the Albian aquifer in Eastern Paris Basin (France, ∼48°N). Our NGT data indicate that the mean annual surface temperature was ∼5 °C during the Marine Isotope Stage 3 (MIS3; ∼40-30 kyr ago), before cooling to ∼2 °C during the LGM, and warming to ∼11 °C in the Holocene, which closely matches modern ground surface temperatures in Eastern France. Combined with water stable isotope analyses, NGT data indicate δD/NGT and δ18O/NGT transfer functions of +1.6 ± 0.4‰/°C and +0.18 ± 0.04‰/°C, respectively. Our noble-gas derived LGM cooling of ∼9 °C (relative to the Holocene) is consistent with previous studies of noble gas paleothermometry in European groundwaters but larger than the low-to-mid latitude estimate of 5.8 ± 0.6 °C derived from a compilation of noble gas records, which supports the notion that continental LGM cooling was more extreme at higher latitudes. While an LGM cooling of ∼9 °C in Eastern France appears compatible with recent data assimilation studies, this value is greater than most estimates from current-generation climate model simulations of the LGM. Comparing our estimate for the temperature in Eastern France during MIS3 (6.4 ± 0.5 °C) with GCM outputs presents a promising avenue to further evaluate climate model simulations and constrain European climate evolution over the last glacial cycle

Integration of self-assembled carbon nanotube transistors: statistics and gate engineering at the wafer scale

International audienc

Integration of self-assembled carbon nanotube transistors: statistics and gate engineering at the wafer scale

International audienc