Identity Change through Affordances Actualization: Evidence from Healthcare Workers

As more and more digital technologies are used in healthcare organizations, the way healthcare workers work and doctor-patient communication are changing. These changes will lead to identity change of healthcare workers. Some scholars try to understand technological changes in terms of the affordance theory. However, there are few relevant studies that incorporate specific application scenarios. In this paper, we explore the specific performance of the digital technology affordance and the impact on healthcare workers’ identity in China. We conducted in-depth interviews with 14 healthcare workers and used grounded theory to summarize three kinds of digital technology affordance, namely functional affordance, process affordance and performance affordance. The findings suggest that on the one hand, digital technology affordance increase the efficiency of healthcare workers and enhance collaboration among colleagues, thus reinforcing the healthcare workers’ identity. On the other hand, over-reliance on digital technology may also lead to unnecessary hassles that worsen healthcare workers’ identity. Our study enriches the affordance theory and identity theory, and has constructive implications for the quality of healthcare services in a digital context

Deqi Sensations of Transcutaneous Electrical Nerve Stimulation on Auricular Points

Deqi sensation, a psychophysical response characterized by a spectrum of different needling sensations, is essential for Chinese acupuncture clinical efficacy. Previous research works have investigated the component of Deqi response upon acupuncture on acupoints on the trunk and limbs. However, the characteristics of Deqi sensations of transcutaneous electrical nerve stimulation (TENS) on auricular points are seldom reported. In this study, we investigated the individual components of Deqi during TENS on auricular concha area and the superior scapha using quantitative measurements in the healthy subjects and depression patients. The most striking characteristics of Deqi sensations upon TENS on auricular points were tingling, numbness, and fullness. The frequencies of pressure, warmness, heaviness, and soreness were relatively lower. The dull pain and coolness are rare. The characteristics of Deqi were similar for the TENS on concha and on the superior scapha

Phytophthora sojae Avirulence Effector Avr3b is a Secreted NADH and ADP-ribose Pyrophosphorylase that Modulates Plant Immunity

Plants have evolved pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) to protect themselves from infection by diverse pathogens. Avirulence (Avr) effectors that trigger plant ETI as a result of recognition by plant resistance (R) gene products have been identified in many plant pathogenic oomycetes and fungi. However, the virulence functions of oomycete and fungal Avr effectors remain largely unknown. Here, we combined bioinformatics and genetics to identify Avr3b, a new Avr gene from Phytophthora sojae, an oomycete pathogen that causes soybean root rot. Avr3b encodes a secreted protein with the RXLR host-targeting motif and C-terminal W and Nudix hydrolase motifs. Some isolates of P. sojae evade perception by the soybean R gene Rps3b through sequence mutation in Avr3b and lowered transcript accumulation. Transient expression of Avr3b in Nicotiana benthamiana increased susceptibility to P. capsici and P. parasitica, with significantly reduced accumulation of reactive oxygen species (ROS) around invasion sites. Biochemical assays confirmed that Avr3b is an ADP-ribose/NADH pyrophosphorylase, as predicted from the Nudix motif. Deletion of the Nudix motif of Avr3b abolished enzyme activity. Mutation of key residues in Nudix motif significantly impaired Avr3b virulence function but not the avirulence activity. Some Nudix hydrolases act as negative regulators of plant immunity, and thus Avr3b might be delivered into host cells as a Nudix hydrolase to impair host immunity. Avr3b homologues are present in several sequenced Phytophthora genomes, suggesting that Phytophthora pathogens might share similar strategies to suppress plant immunity

High Performance of Commercial PAC on the Simultaneous Desulfurization and Denitrification of Wastewater From a Coal-Fired Heating Plant

The flue gas desulfurization wastewater is highly saline and has too many refractory pollutants to be recycled during the desulfurization process of the coal-fired heating plant. Given that waste heat is abundant in coal-fired heating plants, a thermal treatment method was developed to simultaneously remove sulfates and nitrates from the wastewater, with the production of chemical-grade natroalunite and recycled water. The results showed that sulfates and nitrates were 50.3 and 10 g/L in the wastewater, respectively, and only 2.8% and 9.1% were removed after direct treatment at 270°C for 7 h; but these rates increased to 99.3% and 99.9%, respectively, with the addition of commercial poly aluminum chloride. Mass balance summarized that the treatment of 1 ton wastewater needed 0.1 ton PAC and produced 0.11 ton natroalunite and 0.92 ton recycle water. The removal of sulfates and nitrates was mainly done by the precipitation reaction of sulfates such as natroalunite and the redox reaction between nitrates and organics, respectively. Thermodynamic analysis demonstrated that the precipitate reaction occurred at 45°C and accelerated in the temperature range of 45–270°C, but became slow with the decrease of sulfate and Al concentrations in wastewater. Four other reagents were also used for wastewater treatment in comparison with PAC and showed the following order of performance: PAC > citrate calcium > limestone > subacetate aluminum > citrate ferric. This method provided a practical route to treat wastewater from flue gas desulfurization without generating secondary waste

Dcf1 Deficiency Attenuates the Role of Activated Microglia During Neuroinflammation

Microglia serve as the principal immune cells and play crucial roles in the central nervous system, responding to neuroinflammation via migration and the execution of phagocytosis. Dendritic cell-derived factor 1 (Dcf1) is known to play an important role in neural stem cell differentiation, glioma apoptosis, dendritic spine formation, and Alzheimer’s disease (AD), nevertheless, the involvement of the Dcf1 gene in the brain immune response has not yet been reported. In the present paper, the RNA-sequencing and function enrichment analysis suggested that the majority of the down-regulated genes in Dcf1-/- (Dcf1-KO) mice are immune-related. In vivo experiments showed that Dcf1 deletion produced profound effects on microglial function, increased the expression of microglial activation markers, such as ionized calcium binding adaptor molecule 1 (Iba1), Cluster of Differentiation 68 (CD68) and translocator protein (TSPO), as well as certain proinflammatory cytokines (Cxcl1, Ccl7, and IL17D), but decreased the migratory and phagocytic abilities of microglial cells, and reduced the expression levels of some other proinflammatory cytokines (Cox-2, IL-1β, IL-6, TNF-α, and Csf1) in the mouse hippocampus. Furthermore, in vitro experiments revealed that in the absence of lipopolysaccharide (LPS), the majority of microglia were ramified and existed in a resting state, with only approximately 10% of cells exhibiting an amoeboid-like morphology, indicative of an activated state. LPS treatment dramatically increased the ratio of activated to resting cells, and Dcf1 downregulation further increased this ratio. These data indicated that Dcf1 deletion mediates neuroinflammation and induces dysfunction of activated microglia, preventing migration and the execution of phagocytosis. These findings support further investigation into the biological mechanisms underlying microglia-related neuroinflammatory diseases, and the role of Dcf1 in the immune response

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe