Temos que pegar : o jogo Pokémon GO e o potencial de comunicação em 2019

O tema central desse trabalho trata-se de Pokémon, que hoje é denominado a franquia mais rentável de todos os tempos, superando grandes potência do mercado midiático. Neste sentido o texto trará dados históricos e fundamentos básicos necessário para o entendimento desse universo. Como objeto deste trabalho utilizou-se o game mobile Pokémon GO, que em sua própria categoria de mercado, a dos jogos móveis locativos, atingiu marcas sem precedentes. Este trabalho tem por finalidade desvendar as razões para o sucesso da aplicação principalmente no que diz respeito as ações e eventos que ocorreram no ano de 2019. Nessa perspectiva, este trabalho propõe uma conceituação de Jogos para então desvelar as características que colaboram para o impacto dos recursos de interação entre os usuários e a interface para com o mercado. Este trabalho busca, ainda, observar formas diferentes de promover negócios dentro da plataforma, que até o momento não dispõe de um modelo, aberto aos públicos, de comercialização para dentro do ambiente virtual.The central theme of this work is Pokémon, which today is called the highest grossing media franchise of all time, surpassing the very greatests franchises of the media market. In this sense the text will bring historical data and basic foundations necessary for the understanding of this universe. As the object of this work was used the mobile game Pokémon GO, which in its own category of the market, the location-based games, reached unprecedented marks. The purpose of this paper is to unveil the reasons for the success of the application, especially with regard to the actions and events that occurred in the year 2019. From this perspective, this work proposes a conceptualization of Games to then unveil the characteristics that collaborate for the impact of the interaction between users and the interface to the market. This work also seeks to observe different ways of promoting business within the platform, which to date does not have a model, open to the public, of marketing into the virtual environment

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Temos que pegar : o jogo Pokémon GO e o potencial de comunicação em 2019

O tema central desse trabalho trata-se de Pokémon, que hoje é denominado a franquia mais rentável de todos os tempos, superando grandes potência do mercado midiático. Neste sentido o texto trará dados históricos e fundamentos básicos necessário para o entendimento desse universo. Como objeto deste trabalho utilizou-se o game mobile Pokémon GO, que em sua própria categoria de mercado, a dos jogos móveis locativos, atingiu marcas sem precedentes. Este trabalho tem por finalidade desvendar as razões para o sucesso da aplicação principalmente no que diz respeito as ações e eventos que ocorreram no ano de 2019. Nessa perspectiva, este trabalho propõe uma conceituação de Jogos para então desvelar as características que colaboram para o impacto dos recursos de interação entre os usuários e a interface para com o mercado. Este trabalho busca, ainda, observar formas diferentes de promover negócios dentro da plataforma, que até o momento não dispõe de um modelo, aberto aos públicos, de comercialização para dentro do ambiente virtual.The central theme of this work is Pokémon, which today is called the highest grossing media franchise of all time, surpassing the very greatests franchises of the media market. In this sense the text will bring historical data and basic foundations necessary for the understanding of this universe. As the object of this work was used the mobile game Pokémon GO, which in its own category of the market, the location-based games, reached unprecedented marks. The purpose of this paper is to unveil the reasons for the success of the application, especially with regard to the actions and events that occurred in the year 2019. From this perspective, this work proposes a conceptualization of Games to then unveil the characteristics that collaborate for the impact of the interaction between users and the interface to the market. This work also seeks to observe different ways of promoting business within the platform, which to date does not have a model, open to the public, of marketing into the virtual environment

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

International audienceIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field