Clinicopathological And Immunohistochemical Evaluation Of Oral And Oropharyngeal Squamous Cell Carcinoma In Chilean Population

In oral and oropharyngeal squamous cell carcinoma (OCSCC and OPSCC) exist an association between clinical and histopathological parameters with cell proliferation, basal lamina, connective tissue degradation and surrounding stroma markers. We evaluated these associations in Chilean patients. A convenience sample of 37 cases of OCSCC (n=16) and OPSCC (n=21) was analyzed clinically (TNM, clinical stage) and histologically (WHO grade of differentiation, pattern of tumor invasion). We assessed the expression of p53, Ki67, HOXA1, HOXB7, type IV collagen (ColIV) and carcinoma-associated fibroblast (a-SMA-positive cells). Additionally we conducted a univariate/bivariate analysis to assess the relationship of these variables with survival rates. Males were mostly affected (56.2% OCSCC, 76.2% OPSCC). Patients were mainly diagnosed at III/IV clinical stages (68.8% OCSCC, 90.5% OPSCC) with a predominantly infiltrative pattern invasion (62.9% OCSCC, 57.1% OPSCC). Significant association between regional lymph nodes (N) and clinical stage with OCSCC-HOXB7 expression (Chi-Square test P < 0.05) was observed. In OPSCC a statistically significant association exists between p53, Ki67 with gender (Chi-Square test P < 0.05). In OCSCC and OPSCC was statistically significant association between ki67 with HOXA1, HOXB7, and between these last two antigens (Pearson's Correlation test P < 0.05). Furthermore OPSCC-p53 showed significant correlation when it was compared with a-SMA (Kendall's Tau-c test P < 0.05). Only OCSCC-pattern invasion and OPSCC-primary tumor (T) pattern resulted associated with survival at the end of the follow up period (Chi-Square Likelihood Ratio, P < 0.05). Clinical, histological and immunohistochemical features are similar to seen in other countries. Cancer proliferation markers were associated strongly from each other. Our sample highlights prognostic value of T and pattern of invasion, but the conclusions may be limited and should be considered with caution (small sample). 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Medidas morfométricas da carcaça de cordeiros de diferentes genótipos submetidos a dois sistemas de produção.

Resumo: Objetivou-se avaliar a influência do sistema de produção, bem como de diferentes grupos genéticos sobre as características morfométricas da carcaça de cordeiros. Foram observados 24 animais, machos não castrados, das raças ½Dorper x ½SPRD, ½Santa Inês x ½SPRD e ½Somalis x ½SPRD, submetidos à terminação a pasto e em confinamento. Os animais foram abatidos com peso vivo médio de 30 kg. As carcaças foram pesadas e refrigeradas a 4ºC por 24h, quando então foram mensuradas as seguintes medidas morfométricas: comprimento corporal, perímetro torácico, perímetro da perna, comprimento da perna e comprimento interno. O sistema de produção interferiu nas medidas de perímetro da perna e comprimento interno, não havendo diferença significativa (P>0,05) para as demais medidas. Os genótipos estudados não influenciaram na medida de perímetro. As medidas de comprimento da carcaça, perímetro da perna e comprimento interno não diferiram estatisticamente para os mestiços ½Dorper x ½SPRD e ½Santa Inês x ½SPRD, no entanto esses genótipos apresentaram diferença significativa para a raça ½Somalis x ½SPRD nesses parâmetros. O sistema de produção não acarretou mudanças importantes para as medidas morfométricas de comprimento de carcaça, perímetro torácico e comprimento da perna. O mestiço ½Dorper x ½SPRD apresentou as melhores médias para as medidas estudadas. [Morphometric carcass characteristics of lambs of different genotypes under two production systems]. Abstract: Objectified to evaluate the influence of production system, well as of different genetic groups on the morphometric characteristics of the carcass of lambs. 24 animals had been observed, males not castrated, of breeds ½ Dorper x ½ SPRD, ½ Santa Inês x ½ SPRD and ½ Somalis x ½ SPRD, submitted to termination grass and confinement. The animals had been abated with average alive weight 30 kg. The carcasses had been weighed and cooled 4ºC for 24h, when then following morphometric measures had been measured: corporal length, thoracic perimeter, perimeter of leg, length of leg and internal length. The production system intervened with the measures of perimeter of leg and internal length, not having significant difference (P>0.05) for excessively measures. Studied genotypes had not influenced in measure of perimeter. Measures of length of carcass, perimeter of leg and internal length hanot statistical differed for the mestizos ½ Dorper x ½ SPRD and ½ Santa Inês x ½ SPRD, however these genotypes had presented significant difference for the breed ½ Somalis x ½ SPRD in these parameters. The production system does not cause important changes for morphometric measures of carcass length, thoracic perimeter and length of the leg. The mestizo ½ Dorper x ½ SPRD presented best averages for studied measures

Utilización de patrones de estacionalidad e impactos de variables con retardo en el tiempo para la planificación de los nacimientos en sistemas vacunos lecheros.

Con el objetivo de demostrar la posibilidad de utilizar los patrones de comportamiento estacional de las variables con retardos en el tiempo para planificar la mejor época del año para concentrar los partos, se recolectaron todos los boletines mensuales de reproducción correspondientes a las empresas de la cuenca lechera Camagüey-Jimaguayú con las variables año (1982 a 2005), mes (enero a diciembre), hembras inseminadas pendientes a diagnóstico de gestación, hembras recentinas, hembras vacías, incorporaciones a la reproducción, desechos de la reproducción, hembras detectadas en el primer estro, hembras detectadas en total de estros, hembras gestantes en el diagnóstico, hembras no gestantes en el diagnóstico y total de nacimientos. Se realizó el proceso descomposición estacional y el cruce bivariado de las combinaciones entre todas las series en estudio mediante la función de correlación cruzada de series cronológicas utilizando retardos de hasta 24 meses. Fueron determinadas las relaciones de interdependencia de las variables con retardos, mediante la técnica SEM (modelación de ecuaciones estruc-turales). El modelo estructural obtenido, partiendo de un enfoque global utilizando los patrones de comportamiento estacional y las relaciones de dependencia en el tiempo, permitió la planificación de laépoca de los nacimientos mediante la optimización en los escalones precedentes de la variable con mayor impacto en el sistema (RECTOT 10).Using Seasonality Patterns and Time-Lagged Variables Impact for Birth Planning in Dairy Farm-ing SystemABSTRACTThe current study demonstrates the possibility of using seasonal performance patterns derived from time-lagged variables to devise a plan dealing with seasonal calving herds. To this end, data were collected from every monthly bulletin on reproduction from livestock centers in Camagüey-Jimaguayú dairy basin using the following variables: year (1982-2005), month (January-December), inseminated heifers without undergoing a pregnancy diagnosis process, puerperal heifers, non-pregnant heifers, incorporations to reproduction, culled heifers, heifers at first estrus, heifers at total estrus, heifers diagnosed as pregnant, heifers diagnosed as non-pregnant, and total of births. The seasonal de-tached procedure and bivariate crossing of combinations among all the assessed series were performed by crossed correlations of chronological series with lags scored up to 24 months. Lagged variables interdependence relations were determine by the SEM technique (structural equation models). An initial global approach including seasonal performance and time dependent relations was the starting point to find out the structural model to be implemented. This model was useful in planning seasonal births aided by RECTOT 10 software that optimized the highest impact variable

Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors

<p>Abstract</p> <p>Background</p> <p>Odontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine the possible predictive value of these markers.</p> <p>Methods</p> <p>Thirteen cases of RCs and twelve cases of KCOTs were included in this study. Antibodies against laminin-1 and Ki-67 were used as primary antibodies.</p> <p>Results</p> <p>ten cases out of thirteen cases of RCs were immunopositive to laminin-1. The immunonegative cases of RCs showed high degree of inflammation inside the connective tissue wall. One case out of twelve cases of KCOTs was immunopositive to laminin-1 and the rest were immunonegative. Seven cases out of thirteen cases of RCs showed immunopositivity for Ki-67 with increased numbers of immunopositive cells when the inflammation was severe in the connective tissue wall. All KCOTS were immunopositive to Ki-67.</p> <p>Conclusions</p> <p>The benign nature of radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.</p

Search for chargino-neutralino production with mass splittings near the electroweak scale in three-lepton final states in √s=13 TeV pp collisions with the ATLAS detector

A search for supersymmetry through the pair production of electroweakinos with mass splittings near the electroweak scale and decaying via on-shell W and Z bosons is presented for a three-lepton final state. The analyzed proton-proton collision data taken at a center-of-mass energy of √s=13  TeV were collected between 2015 and 2018 by the ATLAS experiment at the Large Hadron Collider, corresponding to an integrated luminosity of 139  fb−1. A search, emulating the recursive jigsaw reconstruction technique with easily reproducible laboratory-frame variables, is performed. The two excesses observed in the 2015–2016 data recursive jigsaw analysis in the low-mass three-lepton phase space are reproduced. Results with the full data set are in agreement with the Standard Model expectations. They are interpreted to set exclusion limits at the 95% confidence level on simplified models of chargino-neutralino pair production for masses up to 345 GeV

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13

Revising the WHO verbal autopsy instrument to facilitate routine cause-of-death monitoring.

OBJECTIVE: Verbal autopsy (VA) is a systematic approach for determining causes of death (CoD) in populations without routine medical certification. It has mainly been used in research contexts and involved relatively lengthy interviews. Our objective here is to describe the process used to shorten, simplify, and standardise the VA process to make it feasible for application on a larger scale such as in routine civil registration and vital statistics (CRVS) systems. METHODS: A literature review of existing VA instruments was undertaken. The World Health Organization (WHO) then facilitated an international consultation process to review experiences with existing VA instruments, including those from WHO, the Demographic Evaluation of Populations and their Health in Developing Countries (INDEPTH) Network, InterVA, and the Population Health Metrics Research Consortium (PHMRC). In an expert meeting, consideration was given to formulating a workable VA CoD list [with mapping to the International Classification of Diseases and Related Health Problems, Tenth Revision (ICD-10) CoD] and to the viability and utility of existing VA interview questions, with a view to undertaking systematic simplification. FINDINGS: A revised VA CoD list was compiled enabling mapping of all ICD-10 CoD onto 62 VA cause categories, chosen on the grounds of public health significance as well as potential for ascertainment from VA. A set of 221 indicators for inclusion in the revised VA instrument was developed on the basis of accumulated experience, with appropriate skip patterns for various population sub-groups. The duration of a VA interview was reduced by about 40% with this new approach. CONCLUSIONS: The revised VA instrument resulting from this consultation process is presented here as a means of making it available for widespread use and evaluation. It is envisaged that this will be used in conjunction with automated models for assigning CoD from VA data, rather than involving physicians

Search for new phenomena in final states with an energetic jet and large missing transverse momentum in pp collisions at √ s = 8 TeV with the ATLAS detector

Results of a search for new phenomena in final states with an energetic jet and large missing transverse momentum are reported. The search uses 20.3 fb−1 of √ s = 8 TeV data collected in 2012 with the ATLAS detector at the LHC. Events are required to have at least one jet with pT > 120 GeV and no leptons. Nine signal regions are considered with increasing missing transverse momentum requirements between Emiss T > 150 GeV and Emiss T > 700 GeV. Good agreement is observed between the number of events in data and Standard Model expectations. The results are translated into exclusion limits on models with either large extra spatial dimensions, pair production of weakly interacting dark matter candidates, or production of very light gravitinos in a gauge-mediated supersymmetric model. In addition, limits on the production of an invisibly decaying Higgs-like boson leading to similar topologies in the final state are presente