Impacts of cutting frequency and position to tree line on herbage accumulation in silvopastoral grassland reveal potential for grassland conservation based on land use and cover information

In agricultural grassland, high herbage utilisation efficiency (HEFF), which is the proportion of gross live-green herbage production that is utilised before entering senescence, is ensured by frequent defoliation. The decision upon which defoliation frequency to apply depends on the farming intensity. Assuming a reduced total herbage accumulation near trees in silvopastoral systems, frequent defoliations with high HEFF become less worthwhile—at least in specific spatial configurations. This makes an extensive management near trees an interesting option because it promotes other grassland-related ecosystem services such as biodiversity. The present study first analysed the interaction between defoliation frequency and position to trees on the total, dead and live herbage accumulation and the HEFF at two silvopastoral sites with short-rotation coppices in Germany. In addition, the total grassland–tree interface in Germany was assessed from land use and land cover maps of Germany based on satellite data to approximate the potential of grassland extensification near trees. The total herbage accumulation near trees declined by up to 41% but the HEFF was not affected by the position. Consequently, any intensification is not paid-off by adequate productivity and herbage quality in terms of HEFF and tree-related losses in herbage accumulation are expected up to a distance of 4.5–6 m. Applying a 4.5 m border on satellite data, we found that up to 4.4% (approximately 2200 km2) of the total grassland area in Germany is at a tree interface and potentially suitable for extensification. These findings indicate substantial potential for biodiversity conservation in grasslands with low trade-off for high-quality yield.Bundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347Peer Reviewe

miR-22 and miR-205 Drive Tumor Aggressiveness of Mucoepidermoid Carcinomas of Salivary Glands

Publisher Copyright: Copyright © 2022 Naakka, Barros-Filho, Adnan-Awad, Al-Samadi, Marchi, Kuasne, Korelin, Suleymanova, Brown, Scapulatempo-Neto, Lourenço, Castilho, Kowalski, Mäkitie, Araújo, Leivo, Rogatto, Salo and Passador-Santos.Objectives: To integrate mRNA and miRNA expression profiles of mucoepidermoid carcinomas (MECs) and normal salivary gland (NSGs) tissue samples and identify potential drivers. Material and Methods: Gene and miRNA expression arrays were performed in 35 MECs and six NSGs. Results: We found 46 differentially expressed (DE) miRNAs and 3,162 DE mRNAs. Supervised hierarchical clustering analysis of the DE transcripts revealed two clusters in both miRNA and mRNA profiles, which distinguished MEC from NSG samples. The integrative miRNA-mRNA analysis revealed a network comprising 696 negatively correlated interactions (44 miRNAs and 444 mRNAs) involving cell signaling, cell cycle, and cancer-related pathways. Increased expression levels of miR-205-5p and miR-224-5p and decreased expression levels of miR-139-3p, miR-145-3p, miR-148a-3p, miR-186-5p, miR-338-3p, miR-363-3p, and miR-4324 were significantly related to worse overall survival in MEC patients. Two overexpressed miRNAs in MEC (miR-22 and miR-205) were selected for inhibition by the CRISPR-Cas9 method. Cell viability, migration, and invasion assays were performed using an intermediate grade MEC cell line. Knockout of miR-205 reduced cell viability and enhanced ZEB2 expression, while miR-22 knockout reduced cell migration and invasion and enhanced ESR1 expression. Our results indicate a distinct transcriptomic profile of MEC compared to NSG, and the integrative analysis highlighted miRNA-mRNA interactions involving cancer-related pathways, including PTEN and PI3K/AKT. Conclusion: The in vitro functional studies revealed that miR-22 and miR-205 deficiencies reduced the viability, migration, and invasion of the MEC cells suggesting they are potential oncogenic drivers in MEC.Peer reviewe

miR-22 and miR-205 Drive Tumor Aggressiveness of Mucoepidermoid Carcinomas of Salivary Glands

ObjectivesTo integrate mRNA and miRNA expression profiles of mucoepidermoid carcinomas (MECs) and normal salivary gland (NSGs) tissue samples and identify potential drivers. Material and MethodsGene and miRNA expression arrays were performed in 35 MECs and six NSGs. ResultsWe found 46 differentially expressed (DE) miRNAs and 3,162 DE mRNAs. Supervised hierarchical clustering analysis of the DE transcripts revealed two clusters in both miRNA and mRNA profiles, which distinguished MEC from NSG samples. The integrative miRNA-mRNA analysis revealed a network comprising 696 negatively correlated interactions (44 miRNAs and 444 mRNAs) involving cell signaling, cell cycle, and cancer-related pathways. Increased expression levels of miR-205-5p and miR-224-5p and decreased expression levels of miR-139-3p, miR-145-3p, miR-148a-3p, miR-186-5p, miR-338-3p, miR-363-3p, and miR-4324 were significantly related to worse overall survival in MEC patients. Two overexpressed miRNAs in MEC (miR-22 and miR-205) were selected for inhibition by the CRISPR-Cas9 method. Cell viability, migration, and invasion assays were performed using an intermediate grade MEC cell line. Knockout of miR-205 reduced cell viability and enhanced ZEB2 expression, while miR-22 knockout reduced cell migration and invasion and enhanced ESR1 expression. Our results indicate a distinct transcriptomic profile of MEC compared to NSG, and the integrative analysis highlighted miRNA-mRNA interactions involving cancer-related pathways, including PTEN and PI3K/AKT. ConclusionThe in vitro functional studies revealed that miR-22 and miR-205 deficiencies reduced the viability, migration, and invasion of the MEC cells suggesting they are potential oncogenic drivers in MEC

Towards long-term standardised carbon and greenhouse gas observations for monitoring Europe's terrestrial ecosystems : a review

Research infrastructures play a key role in launching a new generation of integrated long-term, geographically distributed observation programmes designed to monitor climate change, better understand its impacts on global ecosystems, and evaluate possible mitigation and adaptation strategies. The pan-European Integrated Carbon Observation System combines carbon and greenhouse gas (GHG; CO2, CH4, N2O, H2O) observations within the atmosphere, terrestrial ecosystems and oceans. High-precision measurements are obtained using standardised methodologies, are centrally processed and openly available in a traceable and verifiable fashion in combination with detailed metadata. The Integrated Carbon Observation System ecosystem station network aims to sample climate and land-cover variability across Europe. In addition to GHG flux measurements, a large set of complementary data (including management practices, vegetation and soil characteristics) is collected to support the interpretation, spatial upscaling and modelling of observed ecosystem carbon and GHG dynamics. The applied sampling design was developed and formulated in protocols by the scientific community, representing a trade-off between an ideal dataset and practical feasibility. The use of open-access, high-quality and multi-level data products by different user communities is crucial for the Integrated Carbon Observation System in order to achieve its scientific potential and societal value.Peer reviewe

Peanut‐induced anaphylaxis in children and adolescents: Data from the European Anaphylaxis Registry

Background Peanut allergy has a rising prevalence in high-income countries, affecting 0.5%-1.4% of children. This study aimed to better understand peanut anaphylaxis in comparison to anaphylaxis to other food triggers in European children and adolescents. Methods Data was sourced from the European Anaphylaxis Registry via an online questionnaire, after in-depth review of food-induced anaphylaxis cases in a tertiary paediatric allergy centre. Results 3514 cases of food anaphylaxis were reported between July 2007 - March 2018, 56% in patients younger than 18 years. Peanut anaphylaxis was recorded in 459 children and adolescents (85% of all peanut anaphylaxis cases). Previous reactions (42% vs. 38%; p = .001), asthma comorbidity (47% vs. 35%; p < .001), relevant cofactors (29% vs. 22%; p = .004) and biphasic reactions (10% vs. 4%; p = .001) were more commonly reported in peanut anaphylaxis. Most cases were labelled as severe anaphylaxis (Ring&Messmer grade III 65% vs. 56% and grade IV 1.1% vs. 0.9%; p = .001). Self-administration of intramuscular adrenaline was low (17% vs. 15%), professional adrenaline administration was higher in non-peanut food anaphylaxis (34% vs. 26%; p = .003). Hospitalization was higher for peanut anaphylaxis (67% vs. 54%; p = .004). Conclusions The European Anaphylaxis Registry data confirmed peanut as one of the major causes of severe, potentially life-threatening allergic reactions in European children, with some characteristic features e.g., presence of asthma comorbidity and increased rate of biphasic reactions. Usage of intramuscular adrenaline as first-line treatment is low and needs to be improved. The Registry, designed as the largest database on anaphylaxis, allows continuous assessment of this condition

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Cabbage and fermented vegetables : From death rate heterogeneity in countries to candidates for mitigation strategies of severe COVID-19

Large differences in COVID-19 death rates exist between countries and between regions of the same country. Some very low death rate countries such as Eastern Asia, Central Europe, or the Balkans have a common feature of eating large quantities of fermented foods. Although biases exist when examining ecological studies, fermented vegetables or cabbage have been associated with low death rates in European countries. SARS-CoV-2 binds to its receptor, the angiotensin-converting enzyme 2 (ACE2). As a result of SARS-CoV-2 binding, ACE2 downregulation enhances the angiotensin II receptor type 1 (AT(1)R) axis associated with oxidative stress. This leads to insulin resistance as well as lung and endothelial damage, two severe outcomes of COVID-19. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is the most potent antioxidant in humans and can block in particular the AT(1)R axis. Cabbage contains precursors of sulforaphane, the most active natural activator of Nrf2. Fermented vegetables contain many lactobacilli, which are also potent Nrf2 activators. Three examples are: kimchi in Korea, westernized foods, and the slum paradox. It is proposed that fermented cabbage is a proof-of-concept of dietary manipulations that may enhance Nrf2-associated antioxidant effects, helpful in mitigating COVID-19 severity.Peer reviewe

Nrf2-interacting nutrients and COVID-19 : time for research to develop adaptation strategies

There are large between- and within-country variations in COVID-19 death rates. Some very low death rate settings such as Eastern Asia, Central Europe, the Balkans and Africa have a common feature of eating large quantities of fermented foods whose intake is associated with the activation of the Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) anti-oxidant transcription factor. There are many Nrf2-interacting nutrients (berberine, curcumin, epigallocatechin gallate, genistein, quercetin, resveratrol, sulforaphane) that all act similarly to reduce insulin resistance, endothelial damage, lung injury and cytokine storm. They also act on the same mechanisms (mTOR: Mammalian target of rapamycin, PPAR gamma:Peroxisome proliferator-activated receptor, NF kappa B: Nuclear factor kappa B, ERK: Extracellular signal-regulated kinases and eIF2 alpha:Elongation initiation factor 2 alpha). They may as a result be important in mitigating the severity of COVID-19, acting through the endoplasmic reticulum stress or ACE-Angiotensin-II-AT(1)R axis (AT(1)R) pathway. Many Nrf2-interacting nutrients are also interacting with TRPA1 and/or TRPV1. Interestingly, geographical areas with very low COVID-19 mortality are those with the lowest prevalence of obesity (Sub-Saharan Africa and Asia). It is tempting to propose that Nrf2-interacting foods and nutrients can re-balance insulin resistance and have a significant effect on COVID-19 severity. It is therefore possible that the intake of these foods may restore an optimal natural balance for the Nrf2 pathway and may be of interest in the mitigation of COVID-19 severity

Monitoring drought impacts on grasslands in Central Europe by means of remote sensing time series

Grasländer sind wichtige Elemente der zentraleuropäischen Landschaft und stellen essenzielle Ökosystemdienstleistungen bereit. Dürren, welche durch den globalen Klimawandel zunehmen, haben negative Auswirkungen auf die Vitalität und Produktivität von Grasland. Satellitenmissionen wie Sentinel-2 und Landsat liefern große, bisher ungenutzte Möglichkeiten für das Grasland Monitoring. Ansätze auf Basis quantitativer Parameter, z.B. Prozentanteile von photosynthetisch aktiver Vegetation (PV), nicht photosynthetisch aktiver Vegetation (NPV) und Boden sind bisher für die Anwendung in zentraleuropäischen Grasländern nicht erforscht. Das Ziel der Arbeit war es, das Verständnis von Dürreeinflüssen auf zentraleuropäische Grasländer durch die Entwicklung eines fernerkundungsbasierten Monitoring Frameworks zu verbessern. Der erste Teil dieses Frameworks umfasste die Ableitung konsistenter Zeitreihen von PV-, NPV-, und Bodenanteilen. Der zweite Teil umfasste die Quantifizierung von Dürreeffekten anhand dieser Zeitreihen. Die Ergebnisse zeigten einen großflächigen, massiven und langanhaltenden Rückgang von Graslandvitalität in extremen Dürrejahren (z.B. 2003, 2018-2020). Robuste statistische Zusammenhänge bestätigten die starke Kopplung von Graslandvitalität und Dürre, insbesondere bei gleichzeitigen Hitzewellen. Zudem beeinflussten Bodeneigenschaften sowie klimatische und hydrologische Bedingungen die Dürresensitivität. Die Ergebnisse unterstreichen den Wert von generalisierten Entmischungsansätzen basierend auf Sentinel-2/Landsat Zeitreihen für großflächiges, quantitatives Monitoring von Grasland. Die Ergebnisse deuten darauf hin, dass durch den Klimawandel verstärkte Dürreereignisse in Zukunft erheblichen Einfluss auf die Vitalität von Grasländern in Zentraleuropa haben werden. Die hier gewonnenen Informationen liefern wichtige Beiträge zur Verbesserung von Dürremonitoring und können die Maßnahmenentwicklung zur Verringerung von Dürreschäden im Grasland unterstützen.Grasslands are vital landscape elements in Central Europe providing essential ecosystem services. Drought events, which are increasing with global climate change, negatively affect grassland vitality and productivity. Satellite remote sensing missions such as Sentinel-2/Landsat offer untapped potential for monitoring grassland vitality. However, workflows for grassland monitoring based on fractional cover of photosynthetic vegetation (PV), non-photosynthetic vegetation (NPV), and soil, remain largely unexplored. The goal of this thesis was to advance the understanding of drought impacts on Central European grasslands by developing a framework for monitoring grassland vitality. The framework included the retrieval of consistent PV, NPV, and soil fractional cover time series from Landsat/Sentinel-2, which was achieved by implementing and generalizing an unmixing workflow. Second, drought impacts were quantified and evaluated based on fractional cover time series. Results showed large-scale, severe, and long-lasting negative impacts on grassland vitality in extreme drought years (e.g., in 2003, and 2018-2020). Robust statistical links confirmed the overall consistent coupling of grassland vitality to drought, specifically to compounding droughts and heatwaves. Spatiotemporal patterns of grassland drought sensitivity revealed that underlying factors such as soil features, and climatic and hydrological conditions modulate drought impacts on local to regional scales. Findings of this thesis emphasize the value of generalized unmixing workflows based on Sentinel-2/Landsat time series for quantitative grassland monitoring across large areas. Furthermore, results suggest that droughts amplified by climate change will pose substantial challenges for grassland vitality across Central European grasslands in the future. The findings provide a steppingstone towards improved drought monitoring and can thus inform adaptation efforts to alleviate drought impacts on grasslands

Characterizing spring phenology of temperate broadleaf forests using Landsat and Sentinel-2 time series

Vegetation phenology has a great impact on land-atmosphere interactions like carbon cycling, albedo, and water and energy exchanges. To understand and predict these critical land-atmosphere feedbacks, it is crucial to measure and quantify phenological responses to climate variability, and ultimately climate change. Coarse-resolution sensors such as MODIS and AVHRR have been useful to study vegetation phenology from regional to global scales. These sensors are, however, not capable of discerning phenological variation at moderate spatial scales. By offering increased observation density and higher spatial resolution, the combination of Landsat and Sentinel-2 time series might provide the opportunity to overcome this limitation. In this study, we analyzed the potential of combined Sentinel-2 and Landsat time series for estimating start of season (SOS) of broadleaf forests across Germany for the year 2018. We tested two common statistical modeling approaches (logistic and generalized additive models using thin plate splines) and the two most commonly used vegetation indices, the Normalized Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI). We found strong agreement between SOS estimates from logistic and spline models (rEVI = 0.86; rNDVI = 0.65), whereas agreement was higher for EVI than for NDVI (RMSDEVI = 3.07, RMSDNDVI = 5.26 days). The choice of vegetation index thus had a higher impact on the results than the fitting method. The EVI-based SOS also showed higher correlation with ground observations compared to NDVI (rEVI = 0.51, rNDVI = 0.42). Data density played an important role in estimating land surface phenology. Models combining Sentinel-2A/B, with an average cloud-free observation frequency of 12 days, were largely consistent with the combined Landsat and Sentinel-2 models, suggesting that Sentinel-2A/B may be sufficient to capture SOS for most areas in Germany in 2018. However, in non-overlapping swath areas and mountain areas, observation frequency was significantly lower, underlining the need to combine Landsat and Sentinel-2 for consistent SOS estimates over large areas. Our study demonstrates that estimating SOS of temperate broadleaf forests at medium spatial resolution has become feasible with combined Landsat and Sentinel-2 time series.Peer Reviewe