Risk analysis to support operation and maintenance of an ageing dock-gate for the Port of Marseille Authority

The dry dock n°10 is a strategic infrastructure of the Port of Marseille Authority: its dimensions (465 x 85 m) place it among the biggest dry docks in Europe. Built in the seventies, it is isolated from the sea by a dock-gate in prestressed concrete. Faced to the ageing of this dock-gate, the Port of Marseille Authority wished to make the most of its knowledge in order to assess the feasibility of two considered operation scenarios: use the dry dock for ship repair or for building new civil engineering structures. In this context, OXAND and the Port of Marseille Authority worked together so as to obtain formal and objective decision-making indicators. These indicators took into account the different operation stakes of the dry dock (security, availability), the condition diagnosis and the ageing prognosis of components and facilities of the dock-gate, along with adapted maintenance actions and related costs. The risk analysis methodology used for this study allowed (1) to characterize the risks linked to each foreseen operation scenario, (2) to identify the most appropriate actions to control these risks, integrating operation constraints (e.g.: need to put the dock-gate in dry dock), (3) to rank these actions regarding their cost/benefit ratio and thus (4), to build a maintenance plan

Fuzzy Tandem Repeats Containing p53 Response Elements May Define Species-Specific p53 Target Genes

Evolutionary forces that shape regulatory networks remain poorly understood. In mammals, the Rb pathway is a classic example of species-specific gene regulation, as a germline mutation in one Rb allele promotes retinoblastoma in humans, but not in mice. Here we show that p53 transactivates the Retinoblastoma-like 2 (Rbl2) gene to produce p130 in murine, but not human, cells. We found intronic fuzzy tandem repeats containing perfect p53 response elements to be important for this regulation. We next identified two other murine genes regulated by p53 via fuzzy tandem repeats: Ncoa1 and Klhl26. The repeats are poorly conserved in evolution, and the p53-dependent regulation of the murine genes is lost in humans. Our results indicate a role for the rapid evolution of tandem repeats in shaping differences in p53 regulatory networks between mammalian species

Epreuve d'effort métabolique chez les hémodialysés

LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Risk analysis to support operation and maintenance of an ageing dock-gate for the Port of Marseille Authority

The dry dock n°10 is a strategic infrastructure of the Port of Marseille Authority: its dimensions (465 x 85 m) place it among the biggest dry docks in Europe. Built in the seventies, it is isolated from the sea by a dock-gate in prestressed concrete. Faced to the ageing of this dock-gate, the Port of Marseille Authority wished to make the most of its knowledge in order to assess the feasibility of two considered operation scenarios: use the dry dock for ship repair or for building new civil engineering structures. In this context, OXAND and the Port of Marseille Authority worked together so as to obtain formal and objective decision-making indicators. These indicators took into account the different operation stakes of the dry dock (security, availability), the condition diagnosis and the ageing prognosis of components and facilities of the dock-gate, along with adapted maintenance actions and related costs. The risk analysis methodology used for this study allowed (1) to characterize the risks linked to each foreseen operation scenario, (2) to identify the most appropriate actions to control these risks, integrating operation constraints (e.g.: need to put the dock-gate in dry dock), (3) to rank these actions regarding their cost/benefit ratio and thus (4), to build a maintenance plan

Induction des genes de defense par les elicitines chez le tabac

National audienc

Responses of tobacco to elicitins, proteins from Phytophthora spp. eliciting acquired resistance

International audienc

Mutant mice lacking the p53 C-terminal domain model telomere syndromes

International audienceMutations in p53, although frequent in human cancers , have not been implicated in telomere-related syndromes. Here, we show that homozygous mutant mice expressing p53 D31 , a p53 lacking the C-terminal domain, exhibit increased p53 activity and suffer from aplastic anemia and pulmonary fibrosis, hallmarks of syndromes caused by short telomeres. Indeed, p53 D31/D31 mice had short telomeres and other phenotypic traits associated with the telomere disease dyskeratosis congenita and its severe variant the Hoyeraal-Hreidarsson syndrome. Hetero-zygous p53 +/D31 mice were only mildly affected, but decreased levels of Mdm4, a negative regulator of p53, led to a dramatic aggravation of their symptoms. Importantly, several genes involved in telomere metabolism were downregulated in p53 D31/D31 cells, including Dyskerin, Rtel1, and Tinf2, which are mutated in dyskeratosis congenita, and Terf1, which is implicated in aplastic anemia. Together, these data reveal that a truncating mutation can activate p53 and that p53 plays a major role in the regulation of telomere metabolism