A Report on the Preliminary design of Composite Cocured LCA Fin

A report on the preliminary design of composite cocured LCA fin is presented. A six spar structural configuration involving laminated carbon composite construction is employed in the design of torsion box. The design studies are carried out using a strength of materials based analysis. Three critical loading cases have been considered in the investigation. Results for various cases studied are presented and discussed

Evaluation of damage in foam sandwich components of aircraft

Tn the current set of investigations foam sandwich panels and some components of an aircraft comprising of two layer Glass Fiber Reinforced Plastic(GFRP) face sheets of thickness 1mm each with polyurethene foam as filler of thickness 8mm were examined for detection of debonds and defects. Known defects were introduced in the panels in the form of teflon insert, full foam removal,half foam removal and edge delamination by inserting a teflon and removing it after curing. Two such panels were subjected to acoustic impact and analysis was carried out in both time and frequency domains. These panels were ultrasonically scanned to obtain C-SCAN images as reference to evaluate Acoustic Impact Test (AIT) results. In addition both Fokker bond testing and AIT(woodpecker) were carried out on the same panels and also some critical joints on the actual component. The results obtained from these tests are presented and discussed in this paper

Stress intensity factors for cracks emanating from a pin-loaded lug. 13; 13;

Stress intensity factors (S.I.F.s) for a single radial through crack and for two diametrical through cracks originating from a pin-loaded lug are estimated. Numerical as well as experimental methods are used to obtain these S.I.F.s. Finite Element Analysis (FEA) is used to compute the S.I.F. numerically. In the experimental method, fatigue crack growth tests are conducted to derive the S.I.F. In the F.E. approach, the two dimensional idealization of the lug is modeled with 4 node quadrilateral elements. The stress intensity factor is obtained using modified virtual crack closure integral (MVCCI) technique to post process the FEA data. Nodal forces and displacements in the elements surrounding the crack tip are used to estimate strain energy release rate from which S.I.F. is estimated. In the experimental method, crack growth rate (da/dN) versus stress intensity factor range (DeltaK) correlation data is generated in a single edge notch tension (SENT) specimen of the same thickness (as that of the lug). This data is generated at stress ratio R=0.7 to eliminate the influence of crack closure. In the lug specimen da/dN versus crack length 'a' data is generated at R=0.7. This growth rate is converted to ?K data by referring to da/dN about ?K data of SENT coupon. S.I.F.s derived from these techniques are critically examined

Tumoricidal effects of etoposide incorporated into solid lipid nanoparticles after intraperitoneal administration in Dalton's lymphoma bearing mice

The tumoricidal effects of etoposide incorporated into lipid nanoparticles after single-dose administration were investigated in Dalton's lymphoma ascites bearing mice. Etoposide and its nanoparticle formulations were administered intraperitoneally, and the cell cycle perturbation, cytogenetic damage, cell death (apoptosis), tumor regression, and animal survival were investigated as parameters of response with time. The tumor burden of mice treated with etoposide and its nanoparticle formulations decreased significantly (P<.001) compared with the initial up to 4 to 6 days, followed by an increase at later time intervals. Of the 3 different formulations, the survival time of mice was higher when treated with etoposide-loaded tripalmitin (ETP) nanoparticles, followed by etoposide-loaded glycerol monostearate (EGMS) (27.3%) and etoposide-loaded glycerol distearate (EGDS) (27.3%) compared with free etoposide. Cell cycle analysis revealed the hypodiploid peak (sub G0/G1 cell population) as well as G2 arrest in mice treated with etoposide and its nanoparticle formulations. The frequency of dead cells treated with the nanoparticle formulations remained high even after 8 days of treatment compared with free etoposide. The mice treated with nanoparticle formulations exhibited hypodiploid peaks and reduced S phase even 8 days after treatment, whereas the free etoposide-treated mice showed decrease in apoptosis after 3 days of treatment. The apoptotic frequency in cells 17 days after treatment was in the order of ETP>EGMS>EGDS> etoposide. The experimental results indicated that among the 3 nanoparticle formulations studied, the ETP nanoparticles showed greater and prolonged apoptotic induction properties, resulting in the higher increase in survival time of tumor bearing mice

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

International audienceIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field