ISOLATION AND IDENTIFICATION OF A NEW PHYTOSTEROL FROM HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH LEAVES

Objective: The Plant Holoptelea Intergrifolia (Roxb.) Planch is being used for the treatment of various disorders since time immemorial in the indigenous system of medicine in India. The main objective of the work was to isolate a new phytosterol from petroleum ether extract of Holoptelea  Integrifolia leaves using Preparative Thin Layer Chromatography (TLC). Methods: As per ICH guidelines we have Prepared Thin Layer Chromatographic plates for separation of a new phytosterol from Petroleum  ether extract of leaves of Holoptelea  Intergrifolia (Roxb.) Planch. The mobile phase used for separation of phytosterol consisted ofChloroform: Ethyl acetate, in the volume ratio of 4:6 (v/v), UV, LC/MS, IR and NMR spectral analytical techniques were used for identification and confirmation of structure of a new Phytosterol by Preparative TLC. Results: Preliminary phytochemical analysis of petroleum ether extract of Holoptelea integrifolia leaves showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates. The isolated phytosterol designated as17-(6-(diethylamino) decan-3-yl)-10,13-dimethyl-12,13-dihydro-10H-cyclopenta[a] phenanthren-3-ol. It responded positively to Liebermann Burchard test indicating steroidal nature of the molecule. Conclusion: On the basis of spectral data analysis and chemical reactions, the structure of a new phytosterol isolated by preparative TLC from petroleum ether extract of leaves of Holoptelea Integrifolia (Roxb.) Planch has been formulated by UV, LC/MS, IR and NMR spectral analysis as 17-(6-(diethylamino) decan-3-yl)-10,13-dimethyl-12,13-dihydro-10H-cyclopenta[a] phenanthren-3-ol This is a new phytosterol isolated from plant source and being reported for the first time

GREEN SYNTHESIS OF MAGNETIC IRON NANOPARTICLES USING MEDICINAL PLANT TRIDAX PROCUMBENS LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIMICROBIAL AGENT AGAINST E. COLI

Objective: The present study was aimed at the biological synthesis of magnetic iron nanoparticles by using the plant extract of Tridax procumbens and also to study their antimicrobial property against gram-negative bacteria (Escherichia coli). Methods: The synthesis of magnetic iron nanoparticles was carried out by the co-precipitation method using biological methods like plant extract as reducing agent and capping agents are biocompatible and non-hazardous. These nanoparticles were characterized by UV-Visible spectroscopy, XRD (X-Ray Diffraction), and SEM (Scanning Electron Microscope). As well as antibacterial activity of the nanoparticles was carried out by agar well diffusion method and Most Probable Number (MPN) method against gram-negative E. coli (Escherichia coli) bacteria. Results: The average crystallite size of Magnetic Nanoparticles (MNPs) was found to be 72 nm by X-ray diffraction. The optical absorption band at wavelengths of 240 nm and 402 nm was obtained from the UV Visible spectrum. Spherical shape morphology was observed in SEM studies. The antibacterial assay clearly expressed that E. coli showed a maximum zone of inhibition (15±0.15 mm) at 2 mg/ml and 1 mg/ml concentration was found for Magnetic Nanoparticles. In the Most Probable Number (MPN) test it is seen that the bacterial count is reduced after adding synthesized NPs into the water sample. Conclusion: The results of the present study conclude that the Magnetic Nanoparticles synthesized using Tridax procumbens leaf extracts is found to be stable and show good antibacterial activity against gram-negative (Escherichia coli) bacteria

Electrical and Optical Characterisation of 100 MeV 197Au Irradiated GaAs

Effect of 100 MeV 197Au implantation, followedby Rapid Thermal Annealing on electrical and opticalcharacteristic is reported. Single crystal n+ GaAssubstrates of orientation have been implanted atroom temperature with 197Au ions to the doses of 1X1012,1X1013, 1X1014 ions/cm2. The as-implanted current-voltage(I-V) characteristic of samples is studied and the opticalinvestigations in IR and mid IR-range have been made.The implanted samples were isochronally annealed byRTA system at different temperatures and the roomtemperature electrical characterization and the opticalinvestigations are reported

Electrical Power Line Theft Detection

Recently, India witnessed blackout in the north, north-eastern and the eastern region. This blackout affected 9% of the world’s population and many industrial areas. This power theft detection project is attempt to monitor pilferage of power. Theft is detected and an alert with message is sent to an authorized person. This project is also attempt to improve the current power distribution scenario by using this technology. In this project, we measure and monitor power at various load points in our model. Using that data, we define the load shedding technique through controlled automation. This entire monitoring is happen using a personal computer. There is real time feedback about the faults at generation points, distribution points, and usage points. As this system is also used to measure power and used for load shedding. Hence this project will used in Indian power grid networks as well as in industrial power measurement and control systems

Electrical and Optical Characterisation of 100 MeV 197Au Irradiated GaAs.

Effect of 100 MeV 197Au implantation, followed by Rapid Thermal Annealing on electrical and optical characteristic is reported. Single crystal n+ GaAs substrates of orientation have been implanted at room temperature with 197Au ions to the doses of 1X1012, 1X1013, 1X1014 ions/cm2. The as-implanted current-voltage (I-V) characteristic of samples is studied and the optical investigations in IR and mid IR-range have been made. The implanted samples were isochronally annealed by RTA system at different temperatures and the room temperature electrical characterization and the optical investigations are reported

An overview of anti-diabetic plants used in Gabon: Pharmacology and Toxicology

© 2017 Elsevier B.V. All rights reserved.Ethnopharmacological relevance: The management of diabetes mellitus management in African communities, especially in Gabon, is not well established as more than 60% of population rely on traditional treatments as primary healthcare. The aim of this review was to collect and present the scientific evidence for the use of medicinal plants that are in currect by Gabonese traditional healers to manage diabetes or hyperglycaemia based here on the pharmacological and toxicological profiles of plants with anti-diabetic activity. There are presented in order to promote their therapeutic value, ensure a safer use by population and provide some bases for further study on high potential plants reviewed. Materials and methods: Ethnobotanical studies were sourced using databases such as Online Wiley library, Pubmed, Google Scholar, PROTA, books and unpublished data including Ph.D. and Master thesis, African and Asian journals. Keywords including ‘Diabetes’ ‘Gabon’ ‘Toxicity’ ‘Constituents’ ‘hyperglycaemia’ were used. Results: A total of 69 plants currently used in Gabon with potential anti-diabetic activity have been identified in the literature, all of which have been used in in vivo or in vitro studies. Most of the plants have been studied in human or animal models for their ability to reduce blood glucose, stimulate insulin secretion or inhibit carbohydrates enzymes. Active substances have been identified in 12 out of 69 plants outlined in this review, these include Allium cepa and Tabernanthe iboga. Only eight plants have their active substances tested for anti-diabetic activity and are suitables for further investigation. Toxicological data is scarce and is dose-related to the functional parameters of major organs such as kidney and liver. Conclusion: An in-depth understanding on the pharmacology and toxicology of Gabonese anti-diabetic plants is lacking yet there is a great scope for new treatments. With further research, the use of Gabonese anti-diabetic plants is important to ensure the safety of the diabetic patients in Gabon.Peer reviewedFinal Accepted Versio

Observation of Charge-Dependent Azimuthal Correlations in p-Pb Collisions and Its Implication for the Search for the Chiral Magnetic Effect

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Search for new physics with dijet angular distributions in proton-proton collisions at root S = 13 TeV

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Search for narrow resonances in dilepton mass spectra in proton-proton collisions at root s=13 TeV and combination with 8 TeV data

Peer reviewe

Search for top squark pair production in pp collisions at root s=13 TeV using single lepton events

Peer reviewe