The bilateral deficit during jumping tasks: Relationship with speed and change of direction speed performance

Research to date has investigated the phenomenon of the bilateral deficit (BLD); however, limited research exists on its association with measures of athletic performance. The purpose of the present study was to investigate the magnitude of the BLD and examine its relationship with linear speed and change of direction speed (CODS) performance. Eighteen physically active and healthy university students performed double and single leg countermovement jumps (CMJ), drop jumps (DJ) and standing broad jumps (SBJ), to calculate the BLD across jump tasks. Subjects also performed 10m and 30m sprints and a 505 CODS test, which were correlated with all BLD metrics. Results showed varying levels of BLD across CMJ metrics (jump height, peak force, eccentric impulse, concentric impulse, peak power), DJ metrics (ground contact time, flight time), and the SBJ (distance). However, a bilateral facilitation (BLF) was shown for jump height and reactive strength index (RSI) during the DJ test. The main findings of the present study were that: 1) a larger BLD in CMJ jump height related to a faster 505 change of direction (COD) (left leg) (r = -0.48; p = 0.04), 505 COD (right leg) (r = -0.53; p = 0.02) and COD deficit (right leg) (r = -0.59; p = 0.01), 2) a larger BLD in CMJ concentric impulse related to faster 505 COD (left leg) (r = -0.51; p = 0.03), 505 COD (right leg) (r = -0.64, p = 0.01) and COD deficit (right leg) (r = -0.60; p = 0.01), 3) a larger BLD in DJ flight time related to a faster 505 COD (left leg) (r = -0.48; p = 0.04). These results suggest that a larger BLD is associated with faster CODS performance, but not linear speed. This highlights the individual nature of the BLD and may support the notion of developing movement competency on one limb for enhanced CODS performance

BAT AGN Spectroscopic Survey-III. An observed link between AGN Eddington ratio and narrow emission line ratios

We investigate the observed relationship between black hole mass ($M_{\rm BH}$), bolometric luminosity ($L_{\rm bol}$), and Eddington ratio (${\lambda}_{\rm Edd}$) with optical emission line ratios ([NII] {\lambda}6583/H{\alpha}, [SII] {\lambda}{\lambda}6716,6731/H{\alpha}, [OI] {\lambda}6300/H{\alpha}, [OIII] {\lambda}5007/H{\beta}, [NeIII] {\lambda}3869/H{\beta}, and HeII {\lambda}4686/H{\beta}) of hard X-ray-selected AGN from the BAT AGN Spectroscopic Survey (BASS). We show that the [NII] {\lambda}6583/H{\alpha} ratio exhibits a significant correlation with ${\lambda}_{\rm Edd}$ ($R_{\rm Pear}$ = -0.44, $p$-value=$3\times10^{-13}$, {\sigma} = 0.28 dex), and the correlation is not solely driven by $M_{\rm BH}$ or $L_{\rm bol}$. The observed correlation between [NII] {\lambda}6583/H{\alpha} ratio and $M_{\rm BH}$ is stronger than the correlation with $L_{\rm bol}$, but both are weaker than the ${\lambda}_{\rm Edd}$ correlation. This implies that the large-scale narrow lines of AGN host galaxies carry information about the accretion state of the AGN central engine. We propose that the [NII] {\lambda}6583/H{\alpha} is a useful indicator of Eddington ratio with 0.6 dex of rms scatter, and that it can be used to measure ${\lambda}_{\rm Edd}$ and thus $M_{\rm BH}$ from the measured $L_{\rm bol}$, even for high redshift obscured AGN. We briefly discuss possible physical mechanisms behind this correlation, such as the mass-metallicity relation, X-ray heating, and radiatively driven outflows.Comment: Accepted for publication in MNRAS, 9 pages, 5 figures, 1 tabl

BAT AGN Spectroscopic Survey I: Spectral Measurements, Derived Quantities, and AGN Demographics

We present the first catalog and data release of the Swift-BAT AGN Spectroscopic Survey (BASS). We analyze optical spectra of the majority of AGN (77%, 641/836) detected based on their 14-195 keV emission in the 70-month Swift BAT all-sky catalog. This includes redshift determination, absorption and emission line measurements, and black hole mass and accretion rate estimates for the majority of obscured and un-obscured AGN (74%, 473/641) with 340 measured for the first time. With ~90% of sources at z<0.2, the survey represents a significant census of hard-X-ray selected AGN in the local universe. In this first catalog paper, we describe the spectroscopic observations and datasets, and our initial spectral analysis. The FWHM of the emission lines show broad agreement with the X-ray obscuration (~94%), such that Sy 1-1.8 have NH10^21.9 cm^-2. Seyfert 1.9 show a range of column densities. Compared to narrow line AGN in the SDSS, the X-ray selected AGN have a larger fraction of dusty host galaxies suggesting these types of AGN are missed in optical surveys. Using the most sensitive [OIII]/Hbeta and [NII]/Halpha emission line diagnostic, about half of the sources are classified as Seyferts, ~15% reside in dusty galaxies that lack an Hbeta detection, but for which the line upper limits imply either a Seyfert or LINER, ~15% are in galaxies with weak or no emission lines despite high quality spectra, and a few percent each are LINERS, composite galaxies, HII regions, or in known beamed AGN.Comment: Accepted ApJ, see www.bass-survey.com for dat

BAT AGN spectroscopic survey–II. X-ray emission and high-ionization optical emission lines

We investigate the relationship between X-ray and optical line emission in 340 nearby (z ≃ 0.04) AGN selected above 10 keV using Swift BAT. We find a weak correlation between the extinction corrected [O iii] and hard X-ray luminosity (L^(int)_([OIII])∝L_(14-195) with a large scatter (R_(Pear) = 0.64, σ = 0.62 dex) and a similarly large scatter with the intrinsic 2–10 keV to [O iii] luminosities (R_(Pear) = 0.63, σ = 0.63 dex). Correlations of the hard X-ray fluxes with the fluxes of high-ionization narrow lines ([O iii], He ii, [Ne iii] and [Ne v]) are not significantly better than with the low-ionization lines (H α, [S ii]). Factors like obscuration or physical slit size are not found to be a significant part of the large scatter. In contrast, the optical emission lines show much better correlations with each other (σ = 0.3 dex) than with the X-ray flux. The inherent large scatter questions the common usage of narrow emission lines as AGN bolometric luminosity indicators and suggests that other issues such as geometrical differences in the scattering of the ionized gas or long-term AGN variability are important

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Forster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research

The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy

International audienceThe interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssu-rrna.org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of high-troughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences consists of eight unique taxonomic fields. In total, 136 866 sequences are nuclear encoded, 45 708 (36 501 mitochondrial and 9657 chloroplastic) are from organelles, the remaining being putative chimeric sequences. The website allows the users to download sequences from the entire and partial databases (including representative sequences after clustering at a given level of similarity). Different web tools also allow searches by sequence similarity. The presence of both rRNA and rDNA sequences, taking into account introns (crucial for eukaryotic sequences), a normalized eight terms ranked-taxonomy and updates of new GenBank releases were made possible by a long-term collaboration between experts in taxonomy and computer scientists

The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy

The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssu-rrna.org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of high-troughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences consists of eight unique taxonomic fields. In total, 136 866 sequences are nuclear encoded, 45 708 (36 501 mitochondrial and 9657 chloroplastic) are from organelles, the remaining being putative chimeric sequences. The website allows the users to download sequences from the entire and partial databases (including representative sequences after clustering at a given level of similarity). Different web tools also allow searches by sequence similarity. The presence of both rRNA and rDNA sequences, taking into account introns (crucial for eukaryotic sequences), a normalized eight terms ranked-taxonomy and updates of new GenBank releases were made possible by a long-term collaboration between experts in taxonomy and computer scientist

Using olfactory media cues in e-learning – perspectives from an empirical investigation

People interact with computers using their senses. Currently, in a digital context, traditional digital media like videos and images used to convey information to users, and these media can be used as a source of information. However, relatively few studies have been conducted on olfactory media as a source of information in a digital context. In this paper, we report on a study that examined the possibility of using olfactory media as a source of information and whether its usage as informational cues enhances learning performance and user Quality of Experience (QoE). To this end, an olfactory-enhanced quiz (web-based) was developed about four countries. The quiz contained different types of questions employing four types of digital media in their contents: text, image, audio and olfactory media. Four scents were used that were considered to be related to the respective countries. Sixty-four participants were invited to our experiment to evaluate this application. Our results revealed that usage of olfactory media synchronised with traditional digital media had a significant impact on learner performance compared to the case when no olfactory media was employed. In respect of user QoE, it was found that olfactory media influenced users positively; moreover, they were passionate about engaging with enhanced olfactory applications in the future