Global Interventions for Seismic Upgrading of Substandard RC Buildings

A methodology is developed in this paper for the design and proportioning of interventions for seismic upgrading of substandardreinforced-concrete (RC) buildings. The retrofit approach is presented in the form of a simple design tool that aims toward both demand reduction and enhancement of force and deformation supply through controlled modification of stiffness along the height of the building. This objective is achieved by engineering the translational mode-shape of the structure, so as to optimize the distribution of interstory drift. Resultsfrom the proposed approach are summarized in a spectrum format in which demand, expressed in terms of interstory drift, is related to stiffness. Design charts, which relate the characteristics of commonly used global intervention procedures to influence drift demands, are developed to facilitate the retrofit design. The intervention procedures considered in this paper are reinforced-concrete jacketing, the addition of reinforced concrete walls, and the addition of masonry infills. The proposed methodology is also amenable to adaptation to other strengthening methods, such as the addition of cross-bracing

Fiber-Reinforced Polymer Retrofitting of Predamaged Substandard RC Prismatic Members

An experimental study was conducted to investigate the efficiency of FRP jackets in upgrading the seismic behavior of lightly reinforced concrete prismatic members previously damaged under a combination of axial compression and a reversed cyclic lateral displacement history simulating earthquake effects. The test program comprises 13 cantilever prismatic specimens, which, owing to substandard reinforcing details representative of older construction practices in southern Europe, were susceptible to various undesirable modes of damage such as web-shear cracking, longitudinal bar buckling, or lap-splice failure. After repair, the specimens were retested using the same load combination. The efficiency of the repair options considered in the study, which refer to alternative strengthening systems (with glass or carbon wraps), was investigated with reference to the design parameters of the intervention, the type of the applied lateral displacement history, and the mode of failure that had occurred previously in the initial phase of the tests. The results provided valuable insight regarding participation of the FRP jackets in the various mechanisms of resistance, their ability to reverse the effect of initial damage, and to impart deformation capacity to the structural member

Assessment indices for the seismic vulnerability of existing R.C. buildings

The seismic vulnerability of old multi‐storey reinforced concrete (R.C.) buildings reinforced with substandard details is assessed as a function of interstorey drift demand imposed by the design earthquake while considering brittle termination of elastic response of the critical members of the structure due to a premature shear failure. Interstorey drift demand is related to column and wall translational stiffnesses which are expressed through analytical derivations in terms of the floor area ratios of gravity and lateral load bearing members in the critical floor. Interstorey drift capacity is related to the available transverse reinforcement and the axial load ratio of the vertical members. The significance of the area ratio of vertical members in the typical floor as an index of vulnerability is explored with reference to the limitations in the value of axial load ratio used in R.C. design in order to secure ductile flexural behavior, and also with reference to the stability index of gravity load bearing members. Interstorey Drift Spectra are derived for the existing R.C. buildings suitable for rapid seismic vulnerability screening but also as a guide for rehabilitation of the existing structures. Lightly reinforced or substandard reinforced concrete buildings that reportedly collapsed during previous earthquakes are used as example case studies in order to calibrate the proposed methodology

Design and assessment spectra for retrofitting of RC buildings

This article presents a novel approach for deriving Retrofit Design Spectra (RDS) that are intended for use in preliminary development and assessment of seismic upgrading scenarios of existing structures. The new spectral representation relates the characteristics of the intervention method chosen as the core of the upgrading strategy, with the ductility and strength demand of the retrofitted structure. The methodology utilized for the derivation of the RDS is based on the Capacity Spectrum Method where the capacity curve is described by relationships for global and local intervention methods that are parameterized in terms of fundamental response quantities. The proposed spectra provide direct insight into the complex interrelation between the characteristics of the intervention method and the implications of the upgrading scenario on demand. Alternative retrofit solutions are thus assessed in an efficient way. A case study is used to illustrate practical application of the new approach

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Secondary Metabolites of Marine Microbes: From Natural Products Chemistry to Chemical Ecology

Marine natural products (MNPs) exhibit a wide range of pharmaceutically relevant bioactivities, including antibiotic, antiviral, anticancer, or anti-inflammatory properties. Besides marine macroorganisms such as sponges, algae, or corals, specifically marine bacteria and fungi have shown to produce novel secondary metabolites (SMs) with unique and diverse chemical structures that may hold the key for the development of novel drugs or drug leads. Apart from highlighting their potential benefit to humankind, this review is focusing on the manifold functions of SMs in the marine ecosystem. For example, potent MNPs have the ability to exile predators and competing organisms, act as attractants for mating purposes, or serve as dye for the expulsion or attraction of other organisms. A large compilation of literature on the role of MNPs in marine ecology is available, and several reviews evaluated the function of MNPs for the aforementioned topics. Therefore, we focused the second part of this review on the importance of bioactive compounds from crustose coralline algae (CCA) and their role during coral settlement, a topic that has received less attention. It has been shown that certain SMs derived from CCA and their associated bacteria are able to induce attachment and/or metamorphosis of many benthic invertebrate larvae, including globally threatened reef-building scleractinian corals. This review provides an overview on bioactivities of MNPs from marine microbes and their potential use in medicine as well as on the latest findings of the chemical ecology and settlement process of scleractinian corals and other invertebrate larvae

An experimental and analytical investigation of reinforced concrete beam-column joints strengthened with a range of CFRP schemes applied only to the beam

This paper investigates the experimental and analytical behaviour of beam-column joints that are subjected to a combination of torque, flexural and direct shear forces, where different Carbon Fibre Polymer (CFRP) strengthening wraps have been applied only to the beam. These wrapping schemes have previously been determined by the research community as an effective method of enhancing the torsional capacities of simply supported reinforced concrete beams. In this investigation, four 3/4-scale exterior beam-column joints were subjected to combined monotonic loading; three different beam wrapping schemes were employed to strengthen the beam region of the joint. The paper suggests a series of rational formulae, based on the space truss mechanism, which can be used to evaluate the joint shear demand of the beams wrapped in these various ways. Further, an iterative model, based on the average stress-strain method, has been introduced to predict joint strength. The proposed analytical approaches show good agreement with the experimental results. The experimental outcomes along with the adopted analytical methods reflect the consistent influence of the wrapping ratio, the interaction between the combined forces, the concrete strut capacity and the fibre orientation on the joint forces, the failure mode and the distortion levels. A large rise in the strut force resulting from shear stresses generated from this combination of forces is demonstrated and leads to a sudden-brittle failure. Likewise, increases in the beams’ main steel rebar strains are identified at the column face, again influenced by the load interactions and the wrapping systems used

Effect of bond, aggregate interlock and dowel action on the shear strength degradation of reinforced concrete

Peer reviewed: YesNRC publication: Ye