10 research outputs found
Development of the International Severe Asthma Registry (ISAR) : A Modified Delphi Study
This study is cofunded by Optimum Patient Care Global and AstraZeneca.Peer reviewedPostprin
Large expert-curated database for benchmarking document similarity detection in biomedical literature search
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe
An immunohistochemical study of the expression of cell-cycle-regulated proteins p53, cyclin D1, RB, p27, Ki67 and MSH2 in gallbladder carcinoma and its precursor lesions
Gallbladder carcinomas are rare but highly
lethal neoplasms. We examined the expression of five
cell-cycle-related molecules (p53, RB, cyclin D1, p27,
Ki-67), and MSH2, in 46 carcinomas, 14 adenomas, 15
low-grade dysplasias, 9 intestinal metaplasias and 20
normal gallbladder epithelia. The expression of these
molecules was altered in gallbladder carcinomas and
adenomas. In gallbladder carcinomas we observed
increased expression of p53, cyclin D1, Ki-67, and
MSH2 together with decreased expression of RB and
p27 protein. Aberrant expression of cyclin D1 and
reduced expression of RB were noted in adenomas, and
expression of cyclin D1 was elevated in low-grade
dysplasias. However, there was no change in the levels
of these cell-cycle molecules in metaplasia. Expression
of p53, p27, Ki-67, and MSH2 was correlated with
clinical stage (P<0.05) and there was also a correlation
between the expression of Ki-67 and MSH-2 and patient
age (P<0.05). These results suggest that altered
expression of cell-cycle molecules p53, cyclin D1, RB,
p27, and of MSH-2 is involved in the progression of
gallbladder carcinomas