Exploring the stories of parents from care backgrounds

This study explored how parents from care backgrounds viewed their experiences of childhood. It considered how these experiences influenced parents’ relationships with their children, their concepts and values of parenting and protective factors that contributed to resilience. Six parents were interviewed and gave detailed autobiographical narratives which were transcribed and processed using narrative analysis. Participants were 3 fathers and 3 mothers aged between 25 and 65 years. Participants expressed difficulties in relating to their children consistent with attachment theory, including role confusions, re-traumatisation and over-protectiveness. Despite traumatic childhood experiences participants lacked self-pity and were motivated by a determination to give their children a better experience of childhood than their own. Possible protective factors were indicated in experiences of consistent, stable foster-care and services and personal attributes. Participants described difficulties in making sense of their past lives and reappraised their experiences throughout their narratives. They described managing psychosocial transitions beyond the adolescent changes predicted by focal development theory. This study has implications for clinical practice with families, specifically in raising awareness of the long standing vulnerability that care histories may confer, informing therapeutic practice and the use of integrated models of intervention

Gross solids from combined sewers in dry weather and storms, elucidating production, storage and social factors

Variation in rates of sanitary hygiene products, toilet tissue and faeces occurring in sewers are presented for dry and wet weather from three steep upstream urban catchments with different economic, age and ethnic profiles. Results show, for example, that total daily solids per capita from the low income and ageing populations are almost twice that from high income or ethnic populations. Relative differences are verified through independent questionnaires. The relationship between solids stored in sewers prior to storms, antecedent dry weather period and the proportion of roof to total catchment area is quantified. A full solids' flush occurs when storm flows exceed three times the peak dry weather flow. The data presented will assist urban drainage designers in managing pollution caused by the discharge of sewage solids

Tackling tuberculosis: insights from an international TB Summit in London

Tuberculosis (TB) poses a grave predicament to the world as it is not merely a scientific challenge but a socio-economic burden as well. A prime cause of mortality in human due to an infectious disease; the malady and its cause, Mycobacterium tuberculosis have remained an enigma with many questions that remain unanswered. The ability of the pathogen to survive and switch between varied physiological states necessitates a protracted therapeutic regimen that exerts an excessive strain on low-resource countries. To complicate things further, there has been a significant rise of antimicrobial resistance. Existing control measures, including treatment regimens have remained fairly uniform globally for at least half a century and require reinvention. Overcoming the societal and scientific challenges requires an increase in dialog to identify key regions that need attention and effective partners with whom successful collaborations can be fostered. In this report, we explore the discussions held at the International TB Summit 2015 hosted by EuroSciCon, which served as an excellent platform for researchers to share their recent findings. Ground-breaking results require outreach to affect policy design, governance and control of the disease. Hence, we feel it is important that meetings such as these reach a wider, global audience

Parameter Estimation for Stellar-Origin Black Hole Mergers In LISA

The population of stellar origin black hole binaries (SOBHBs) detected by existing ground-based gravitational wave detectors is an exciting target for the future space-based Laser Interferometer Space Antenna (LISA). LISA is sensitive to signals at significantly lower frequencies than ground-based detectors. SOBHB signals will thus be detected much earlier in their evolution, years to decades before they merge. The mergers will then occur in the frequency band covered by ground-based detectors. Observing SOBHBs years before merger can help distinguish between progenitor models for these systems. We present a new Bayesian parameter estimation algorithm for LISA observations of SOBHBs that uses a time-frequency (wavelet) based likelihood function. Our technique accelerates the analysis by several orders of magnitude compared to the standard frequency domain approach and allows for an efficient treatment of non-stationary noise.Comment: 13 pages, 6 figures, 1 tabl

LISA Galactic Binaries in the Roman Galactic Bulge Time-Domain Survey

Short-period Galactic white dwarf binaries detectable by LISA are the only guaranteed persistent sources for multi-messenger gravitational-wave astronomy. Large-scale surveys in the 2020s present an opportunity to conduct preparatory science campaigns to maximize the science yield from future multi-messenger targets. The Nancy Grace Roman Space Telescope Galactic Bulge Time Domain Survey will (in its Reference Survey design) image seven fields in the Galactic Bulge approximately 40,000 times each. Although the Reference Survey cadence is optimized for detecting exoplanets via microlensing, it is also capable of detecting short-period white dwarf binaries. In this paper, we present forecasts for the number of detached short-period binaries the Roman Galactic Bulge Time Domain Survey will discover and the implications for the design of electromagnetic surveys. Although population models are highly uncertain, we find a high probability that the baseline survey will detect of order ~5 detached white dwarf binaries. The Reference Survey would also have a $\gtrsim20\%$ chance of detecting several known benchmark white dwarf binaries at the distance of the Galactic Bulge.Comment: 9 pages, 4 figure, 1 tabl

LISA Gravitational Wave Sources in A Time-Varying Galactic Stochastic Background

A unique challenge for data analysis with the Laser Interferometer Space Antenna (LISA) is that the noise backgrounds from instrumental noise and astrophysical sources will change significantly over both the year and the entire mission. Variations in the noise levels will be on time scales comparable to, or shorter than, the time most signals spend in the detector's sensitive band. The variation in the amplitude of the galactic stochastic GW background from galactic binaries as the antenna pattern rotates relative to the galactic center is a particularly significant component of the noise variation. LISA's sensitivity to different source classes will therefore vary as a function of sky location and time. The variation will impact both overall signal-to-noise and the efficiency of alerts to EM observers to search for multi-messenger counterparts.Comment: 15 pages, 9 figures, 3 table

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Forster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research

Chemical diversity in a metal-organic framework revealed by fluorescence lifetime imaging

The presence and variation of chemical functionality and defects in crystalline materials, such as metal–organic frameworks (MOFs), have tremendous impact on their properties. Finding a means of identifying and characterizing this chemical diversity is an important ongoing challenge. This task is complicated by the characteristic problem of bulk measurements only giving a statistical average over an entire sample, leaving uncharacterized any diversity that might exist between crystallites or even within individual crystals. Here we show that by using fluorescence imaging and lifetime analysis, both the spatial arrangement of functionalities and the level of defects within a multivariable MOF crystal can be determined for the bulk as well as for the individual constituent crystals. We apply these methods to UiO-67, to study the incorporation of functional groups and their consequences on the structural features. We believe that the potential of the techniques presented here in uncovering chemical diversity in what is generally assumed to be homogeneous systems can provide a new level of understanding of materials properties

Personality of wild male crested macaques (Macaca nigra).

Animal personalities, i.e. consistent differences in behavior across time and/or context, have received increased attention of behavioral biologists over the last years. Recent research shows that personalities represent traits on which natural and sexual selection work and which can have substantial fitness consequences. The aim of this study is to establish the personality structure of crested macaque (Macaca nigra) males as foundation for future studies on its adaptive value. We collected behavioral data through focal animal sampling and additionally conducted two sets of playback experiments. Results of a factor analysis on the behavioral data revealed a four factor structure with components we labeled Anxiety, Sociability, Connectedness and Aggressiveness. Results from the experiments revealed an additional and independent Boldness factor but the absence of Neophilia. Overall, this structure resembles other macaque and animal species with the exception of Connectedness, which might be a consequence of the species' tolerant social style. Our results thus not only form the basis for future studies on the adaptive value of personality in crested macaques but also contribute an important data point for investigating the evolution of personality structure from a comparative perspective by refining, for example, which personality factors characterized the last common ancestor of hominids and macaques