Humans have already increased the risk of major disruptions to Pacific rainfall

© The Author(s) 2017.Intermittent disruptions to rainfall patterns and intensity over the Pacific Ocean lasting up to ∼ 1 year have major impacts on severe weather, agricultural production, ecosystems, and disease within the Pacific, and in many countries beyond. The frequency with which major disruptions to Pacific rainfall occur has been projected to increase over the 21st century, in response to global warming caused by large 21st century greenhouse gas emissions. Here we use the latest generation of climate models to show that humans may have contributed to the major disruption that occurred in the real world during the late 20th century. We demonstrate that although marked and sustained reductions in 21st century anthropogenic greenhouse gas emissions can greatly moderate the likelihood of major disruption, elevated risk of occurrence appears locked in now, and for at least the remainder of the 21st century

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

The impact of global warming and the El Nino-Southern Oscillation on seasonal precipitation extremes in Australia

© 2020, The Author(s).The El Niño-Southern Oscillation (ENSO) drives substantial variability in precipitation and drought risk over Australia. Understanding the combined effect of anthropogenic forcing and ENSO on Australian precipitation extremes over the coming century can assist adaptation efforts. Here we use 24 CMIP5 climate models to examine externally forced changes in the frequency of 'droughts', when precipitation falls below the pre-industrial Decile 1 threshold. We focus on June to November (i.e., southern hemisphere Winter–Spring season) because precipitation during this period is important for agricultural production and recharging reservoirs in many parts of the country. The analysis in this paper is based on two 90-year simulations (1900–1989 and 2010–2099) for Historical and RCP8.5 scenarios. We show that the frequency of droughts, including droughts occurring in consecutive Winter–Spring seasons, is projected to increase in the twenty-first century under the RCP8.5 scenario in all eight Natural Resource Management (NRM) 'clusters'. Approximately 60% of years are projected to be drought years in Perth, 35% in Adelaide, 30% in Melbourne, and approximately 20–25% of years in Sydney, Canberra and Brisbane. The relative frequency distributions of seasonally averaged Winter–Spring precipitation shift to lower values in all NRM clusters. However, apart from the Southern and Southwestern Flatlands, the shifts are accompanied by changes in the shape of the distributions whereby the high end of the distributions do not shift as much as other parts of the distribution and the wettest seasons become marginally wetter. This means that in most locations generally drier conditions are projected to be infrequently punctuated by seasons that are just as wet or wetter than the wettest years experienced during the twentieth century. While the models generally do a poor job in simulating ENSO precipitation teleconnections over Australia, an increase in ENSO-driven variability is suggested for the Wet Tropics, the Monsoonal North, the Central Slopes and the Southern and Southwestern Flatlands

Setting and smashing extreme temperature records over the coming century

Changes in the intensity or frequency of extreme climate events can profoundly increase the disruption caused by climate change1–4. The more extreme these events, the greater the potential to push ecosystems and communities beyond their ability to cope3,5. The rate at which existing high temperature records have been broken has increased in response to rising global greenhouse gas emissions (GGHGEs)2,6–8, and the rate at which historical records are surpassed is projected to increase further over the coming century1,2,9,10. Here we examine future events that will be so extreme that they will not have been experienced previously. Record setting in 22 climate models11 indicates that, by the end of the twenty-first century, under business-as-usual increases in GGHGEs (representative concentration pathway (RCP) 8.5 (ref. 12)), high monthly mean temperature records will be set in approximately 58% of the world every year, and in 67% of least developed countries and 68% of small island developing states. These figures all drop to 14% under a scenario with much lower GHG concentrations (RCP2.6 (ref. 12)). In any given year, the likelihood of ‘smashing’ at least one monthly record by more than 1.0 °C is much less likely under RCP2.6 than it is under RCP8.5 (1.1 versus 8.9%)

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

No abstract available

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present