Recommendations for the advancement of oil-in-water media and source oil characterization in aquatic toxicity test studies

During toxicity testing, chemical analyses of oil and exposure media samples are needed to allow comparison of results between different tests as well as to assist with identification of the drivers and mechanisms for the toxic effects observed. However, to maximize the ability to compare results between different laboratories and biota, it has long been recognized that guidelines for standard protocols were needed. In 2005, the Chemical Response to Oil Spills: Ecological Effects Research Forum (CROSERF) protocol was developed with existing common analytical methods that described a standard method for reproducible preparation of exposure media as well as recommended specific analytical methods and analyte lists for comparative toxicity testing. At the time, the primary purpose for the data collected was to inform oil spill response and contingency planning. Since then, with improvements in both analytical equipment and methods, the use of toxicity data has expanded to include their integration into fate and effect models that aim to extend the applicability of lab-based study results to make predictions for field system-level impacts. This paper focuses on providing a summary of current chemical analyses for characterization of oil and exposure media used during aquatic toxicity testing and makes recommendations for the minimum analyses needed to allow for interpretation and modeling purposes.publishedVersio

Index-based approach for estimating vulnerability of Arctic biota to oil spills

Risk of an Arctic oil spill has become a global matter of concern. Climate change induced opening of shipping routes increases the Arctic maritime traffic which exposes the area to negative impacts of potential maritime accidents. Still, quantitative analyses of the likely environmental impacts of such accidents are scarce, and our understanding of the uncertainties related to both accidents and their consequences is poor. There is an obvious need for analysis tools that allow us to systematically analyze the impacts of oil spills on Arctic species, so the risks can be taken into account when new sea routes or previously unexploited oil reserves are utilized. In this paper, an index‐based approach is developed to study exposure potential (described via probability of becoming exposed to spilled oil) and sensitivity (described via oil‐induced mortality and recovery) of Arctic biota in the face of an oil spill. First, a conceptual model presenting the relevant variables that contribute to exposure potential and sensitivity of key Arctic marine functional groups was built. Second, based on an extensive literature review, a probabilistic estimate was assigned for each variable, and the variables were combined to an index representing the overall vulnerability of Arctic biota. The resulting index can be used to compare the relative risk between functional groups and accident scenarios. Results indicate that birds have the highest vulnerability to spilled oil, and seals and whales the lowest. Polar bears’ vulnerability varies greatly between seasons, while ice seals’ vulnerability remains the same in every accident scenario. Exposure potential of most groups depends strongly on type of oil, whereas their sensitivity contains less variation.Peer reviewe

The Influence of Manga on the Graphic Novel

This material has been published in The Cambridge History of the Graphic Novel edited by Jan Baetens, Hugo Frey, Stephen E. Tabachnick. This version is free to view and download for personal use only. Not for re-distribution, re-sale or use in derivative works. © Cambridge University PressProviding a range of cogent examples, this chapter describes the influences of the Manga genre of comics strip on the Graphic Novel genre, over the last 35 years, considering the functions of domestication, foreignisation and transmedia on readers, markets and forms

Quantification of Oil Spill Risk

The identification and evaluation of oil spill risk is important for contingency planning, as well as for the decision-making processes inherent in spill risk management. It needs to encompass both the probability of oil spills occurring under a number of types of circumstances, along with the potential consequences or impacts of the oil spillage. The quantification of oil spill risk provides policy makers and officials with more objective measures of probabilities, consequences, and overall risk to make informed decisions. Each potential source of oil spillage presents its own challenges for measuring the components of risk. This chapter presents state-of-the-art approaches to risk quantification for four varied spill sources-vessels, oil wells, sunken shipwrecks, and crude oil trains to demonstrate varied approaches.For vessel spills, risk analysis includes calculating the probabilities of vessel accidents that may result in spills through vessel traffic studies, coupled with outflow analyses that determine the probability of spillage and the volume of oil released. The consequences of vessel spills can be quantified through oil spill trajectory, fate, and effects modeling.Determining the probability and magnitude of well blowouts can be accomplished through the application of a fault-tree model. Again, the consequences of spillage can be determined with oil spill trajectory, fate, and effects modeling.Sunken wrecks containing oil present a unique form of spill risk. The wrecks may or may not leak or release oil in some future time until corrosion or disturbance breaks the vessels\u27 bunker or oil cargo tanks. The probability of spillage is dependent on evaluating the factors that may lead to a release. Spill trajectory, fate, and effects modeling can be used to predict potential spill consequences.The dramatic increase in the use of unit trains to transport crude oil in large quantities, coupled with the potential for accidental spills with devastating consequences of fire and explosion, have led to an urgent need to quantify risk. Again, modeling tools can be used to assist decision makers and planners in assessing this risk

Improving the design and conduct of aquatic toxicity studies with oils based on 20 years of CROSERF experience

Laboratory toxicity testing is a key tool used in oil spill science, spill effects assessment, and mitigation strategy decisions to minimize environmental impacts. A major consideration in oil toxicity testing is how to replicate real-world spill conditions, oil types, weathering states, receptor organisms, and modifying environmental factors under laboratory conditions. Oils and petroleum-derived products are comprised of thousands of compounds with different physicochemical and toxicological properties, and this leads to challenges in conducting and interpreting oil toxicity studies. Experimental methods used to mix oils with aqueous test media have been shown to influence the aqueous-phase hydrocarbon composition and concentrations, hydrocarbon phase distribution (i. e., dissolved phase versus in oil droplets), and the stability of oil:water solutions which, in turn, influence the bioavailability and toxicity of the oil containing media. Studies have shown that differences in experimental methods can lead to divergent test results. Therefore, it is imperative to standardize the methods used to prepare oil:water solutions in order to improve the realism and comparability of laboratory tests. The CROSERF methodology, originally published in 2005, was developed as a standardized method to prepare oil:water solutions for testing and evaluating dispersants and dispersed oil. However, it was found equally applicable for use in testing oil-derived petroleum substances. The goals of the current effort were to: (1) build upon two decades of experience to update existing CROSERF guidance for conducting aquatic toxicity tests and (2) to improve the design of laboratory toxicity studies for use in hazard evaluation and development of quantitative effects models that can then be applied in spill assessment. Key experimental design considerations discussed include species selection (standard vs field collected), test substance (single compound vs whole oil), exposure regime (static vs flow-through) and duration, exposure metrics, toxicity endpoints, and quality assurance and control