116 research outputs found
Cell and matrix response of temporomandibular cartilage to mechanical loading
OBJECTIVES:
The generation of transgenic mice expressing green fluorescent proteins (GFPs) has greatly aided our understanding of the development of connective tissues such as bone and cartilage. Perturbation of a biological system such as the temporomandibular joint (TMJ) within its adaptive remodeling capacity is particularly useful in analyzing cellular lineage progression. The objectives of this study were to determine: (i) if GFP reporters expressed in the TMJ indicate the different stages of cell maturation in fibrocartilage and (ii) how mechanical loading affects cellular response in different regions of the cartilage.
DESIGN/METHODS:
Four-week-old transgenic mice harboring combinations of fluorescent reporters (Dkk3-eGFP, Col1a1(3.6 kb)-GFPcyan, Col1a1(3.6 kb)-GFPtpz, Col2a1-GFPcyan, and Col10a1-RFPcherry) were used to analyze the expression pattern of transgenes in the mandibular condylar cartilage (MCC). To study the effect of TMJ loading, animals were subjected to forced mouth opening with custom springs exerting 50 g force for 1 h/day for 5 days. Dynamic mineralization and cellular proliferation (EdU-labeling) were assessed in loaded vs control mice.
RESULTS:
Dkk3 expression was seen in the superficial zone of the MCC, followed by Col1 in the cartilage zone, Col2 in the prehypertrophic zone, and Col10 in the hypertrophic zone at and below the tidemark. TMJ loading increased expression of the GFP reporters and EdU-labeling of cells in the cartilage, resulting in a thickness increase of all layers of the cartilage. In addition, mineral apposition increased resulting in Col10 expression by unmineralized cells above the tidemark.
CONCLUSION:
The TMJ responded to static loading by forming thicker cartilage through adaptive remodeling
Simultaneous Planck, Swift, and Fermi observations of X-ray and gamma-ray selected blazars
We present simultaneous Planck, Swift, Fermi, and ground-based data for 105
blazars belonging to three samples with flux limits in the soft X-ray, hard
X-ray, and gamma-ray bands. Our unique data set has allowed us to demonstrate
that the selection method strongly influences the results, producing biases
that cannot be ignored. Almost all the BL Lac objects have been detected by
Fermi-LAT, whereas ~40% of the flat-spectrum radio quasars (FSRQs) in the
radio, soft X-ray, and hard X-ray selected samples are still below the
gamma-ray detection limit even after integrating 27 months of Fermi-LAT data.
The radio to sub-mm spectral slope of blazars is quite flat up to ~70GHz, above
which it steepens to ~-0.65. BL Lacs have significantly flatter spectra
than FSRQs at higher frequencies. The distribution of the rest-frame
synchrotron peak frequency (\nupS) in the SED of FSRQs is the same in all the
blazar samples with =10^13.1 Hz, while the mean inverse-Compton peak
frequency, , ranges from 10^21 to 10^22 Hz. The distributions of \nupS
and of \nupIC of BL Lacs are much broader and are shifted to higher energies
than those of FSRQs and strongly depend on the selection method. The Compton
dominance of blazars ranges from ~0.2 to ~100, with only FSRQs reaching values
>3. Its distribution is broad and depends strongly on the selection method,
with gamma-ray selected blazars peaking at ~7 or more, and radio-selected
blazars at values ~1, thus implying that the assumption that the blazar power
is dominated by high-energy emission is a selection effect. Simple SSC models
cannot explain the SEDs of most of the gamma-ray detected blazars in all
samples. The SED of the blazars that were not detected by Fermi-LAT may instead
be consistent with SSC emission. Our data challenge the correlation between
bolometric luminosity and \nupS predicted by the blazar sequence.Comment: Version accepted by A&A. Joint Planck, Swift, and Fermi
collaborations pape
Planck early results XV : Spectral energy distributions and radio continuum spectra of northern extragalactic radio sources
Peer reviewe
Planck early results. XV. Spectral energy distributions and radio continuum spectra of northern extragalactic radio sources
Spectral energy distributions (SEDs) and radio continuum spectra are presented for a northern sample of 104 extragalactic radio sources, based
on the Planck Early Release Compact Source Catalogue (ERCSC) and simultaneous multifrequency data. The nine Planck frequencies, from 30
to 857 GHz, are complemented by a set of simultaneous observations ranging from radio to gamma-rays. This is the first extensive frequency
coverage in the radio and millimetre domains for an essentially complete sample of extragalactic radio sources, and it shows how the individual
shocks, each in their own phase of development, shape the radio spectra as they move in the relativistic jet. The SEDs presented in this paper
were fitted with second and third degree polynomials to estimate the frequencies of the synchrotron and inverse Compton (IC) peaks, and the
spectral indices of low and high frequency radio data, including the Planck ERCSC data, were calculated. SED modelling methods are discussed,
with an emphasis on proper, physical modelling of the synchrotron bump using multiple components. Planck ERCSC data also suggest that the
original accelerated electron energy spectrum could be much harder than commonly thought, with power-law index around 1.5 instead of the
canonical 2.5. The implications of this are discussed for the acceleration mechanisms effective in blazar shocks. Furthermore in many cases the
Planck data indicate that gamma-ray emission must originate in the same shocks that produce the radio emission
Virgo Detector Characterization and Data Quality during the O3 run
The Advanced Virgo detector has contributed with its data to the rapid growth
of the number of detected gravitational-wave signals in the past few years,
alongside the two LIGO instruments. First, during the last month of the
Observation Run 2 (O2) in August 2017 (with, most notably, the compact binary
mergers GW170814 and GW170817) and then during the full Observation Run 3 (O3):
an 11 months data taking period, between April 2019 and March 2020, that led to
the addition of about 80 events to the catalog of transient gravitational-wave
sources maintained by LIGO, Virgo and KAGRA. These discoveries and the manifold
exploitation of the detected waveforms require an accurate characterization of
the quality of the data, such as continuous study and monitoring of the
detector noise. These activities, collectively named {\em detector
characterization} or {\em DetChar}, span the whole workflow of the Virgo data,
from the instrument front-end to the final analysis. They are described in
details in the following article, with a focus on the associated tools, the
results achieved by the Virgo DetChar group during the O3 run and the main
prospects for future data-taking periods with an improved detector.Comment: 86 pages, 33 figures. This paper has been divided into two articles
which supercede it and have been posted to arXiv on October 2022. Please use
these new preprints as references: arXiv:2210.15634 (tools and methods) and
arXiv:2210.15633 (results from the O3 run
Virgo Detector Characterization and Data Quality: results from the O3 run
The Advanced Virgo detector has contributed with its data to the rapid growth
of the number of detected gravitational-wave (GW) signals in the past few
years, alongside the two Advanced LIGO instruments. First during the last month
of the Observation Run 2 (O2) in August 2017 (with, most notably, the compact
binary mergers GW170814 and GW170817), and then during the full Observation Run
3 (O3): an 11-months data taking period, between April 2019 and March 2020,
that led to the addition of about 80 events to the catalog of transient GW
sources maintained by LIGO, Virgo and now KAGRA. These discoveries and the
manifold exploitation of the detected waveforms require an accurate
characterization of the quality of the data, such as continuous study and
monitoring of the detector noise sources. These activities, collectively named
{\em detector characterization and data quality} or {\em DetChar}, span the
whole workflow of the Virgo data, from the instrument front-end hardware to the
final analyses. They are described in details in the following article, with a
focus on the results achieved by the Virgo DetChar group during the O3 run.
Concurrently, a companion article describes the tools that have been used by
the Virgo DetChar group to perform this work.Comment: 57 pages, 18 figures. To be submitted to Class. and Quantum Grav.
This is the "Results" part of preprint arXiv:2205.01555 [gr-qc] which has
been split into two companion articles: one about the tools and methods, the
other about the analyses of the O3 Virgo dat
Virgo Detector Characterization and Data Quality: tools
Detector characterization and data quality studies -- collectively referred
to as {\em DetChar} activities in this article -- are paramount to the
scientific exploitation of the joint dataset collected by the LIGO-Virgo-KAGRA
global network of ground-based gravitational-wave (GW) detectors. They take
place during each phase of the operation of the instruments (upgrade, tuning
and optimization, data taking), are required at all steps of the dataflow (from
data acquisition to the final list of GW events) and operate at various
latencies (from near real-time to vet the public alerts to offline analyses).
This work requires a wide set of tools which have been developed over the years
to fulfill the requirements of the various DetChar studies: data access and
bookkeeping; global monitoring of the instruments and of the different steps of
the data processing; studies of the global properties of the noise at the
detector outputs; identification and follow-up of noise peculiar features
(whether they be transient or continuously present in the data); quick
processing of the public alerts. The present article reviews all the tools used
by the Virgo DetChar group during the third LIGO-Virgo Observation Run (O3,
from April 2019 to March 2020), mainly to analyse the Virgo data acquired at
EGO. Concurrently, a companion article focuses on the results achieved by the
DetChar group during the O3 run using these tools.Comment: 44 pages, 16 figures. To be submitted to Class. and Quantum Grav.
This is the "Tools" part of preprint arXiv:2205.01555 [gr-qc] which has been
split into two companion articles: one about the tools and methods, the other
about the analyses of the O3 Virgo dat
Safety of intravenous ferric carboxymaltose versus oral iron in patients with nondialysis-dependent CKD: an analysis of the 1-year FIND-CKD trial.
Background: The evidence base regarding the safety of intravenous (IV) iron therapy in patients with chronic kidney disease (CKD) is incomplete and largely based on small studies of relatively short duration. Methods: FIND-CKD (ClinicalTrials.gov number NCT00994318) was a 1-year, open-label, multicenter, prospective study of patients with nondialysis-dependent CKD, anemia and iron deficiency randomized (1:1:2) to IV ferric carboxymaltose (FCM), targeting higher (400-600 µg/L) or lower (100-200 µg/L) ferritin, or oral iron. A post hoc analysis of adverse event rates per 100 patient-years was performed to assess the safety of FCM versus oral iron over an extended period. Results: The safety population included 616 patients. The incidence of one or more adverse events was 91.0, 100.0 and 105.0 per 100 patient-years in the high ferritin FCM, low ferritin FCM and oral iron groups, respectively. The incidence of adverse events with a suspected relation to study drug was 15.9, 17.8 and 36.7 per 100 patient-years in the three groups; for serious adverse events, the incidence was 28.2, 27.9 and 24.3 per 100 patient-years. The incidence of cardiac disorders and infections was similar between groups. At least one ferritin level ≥800 µg/L occurred in 26.6% of high ferritin FCM patients, with no associated increase in adverse events. No patient with ferritin ≥800 µg/L discontinued the study drug due to adverse events. Estimated glomerular filtration rate remained the stable in all groups. Conclusions: These results further support the conclusion that correction of iron deficiency anemia with IV FCM is safe in patients with nondialysis-dependent CKD
Mortality and pulmonary complications in patients undergoing surgery with perioperative SARS-CoV-2 infection: an international cohort study
Background: The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on postoperative recovery needs to be understood to inform clinical decision making during and after the COVID-19 pandemic. This study reports 30-day mortality and pulmonary complication rates in patients with perioperative SARS-CoV-2 infection. Methods: This international, multicentre, cohort study at 235 hospitals in 24 countries included all patients undergoing surgery who had SARS-CoV-2 infection confirmed within 7 days before or 30 days after surgery. The primary outcome measure was 30-day postoperative mortality and was assessed in all enrolled patients. The main secondary outcome measure was pulmonary complications, defined as pneumonia, acute respiratory distress syndrome, or unexpected postoperative ventilation. Findings: This analysis includes 1128 patients who had surgery between Jan 1 and March 31, 2020, of whom 835 (74·0%) had emergency surgery and 280 (24·8%) had elective surgery. SARS-CoV-2 infection was confirmed preoperatively in 294 (26·1%) patients. 30-day mortality was 23·8% (268 of 1128). Pulmonary complications occurred in 577 (51·2%) of 1128 patients; 30-day mortality in these patients was 38·0% (219 of 577), accounting for 81·7% (219 of 268) of all deaths. In adjusted analyses, 30-day mortality was associated with male sex (odds ratio 1·75 [95% CI 1·28–2·40], p\textless0·0001), age 70 years or older versus younger than 70 years (2·30 [1·65–3·22], p\textless0·0001), American Society of Anesthesiologists grades 3–5 versus grades 1–2 (2·35 [1·57–3·53], p\textless0·0001), malignant versus benign or obstetric diagnosis (1·55 [1·01–2·39], p=0·046), emergency versus elective surgery (1·67 [1·06–2·63], p=0·026), and major versus minor surgery (1·52 [1·01–2·31], p=0·047). Interpretation: Postoperative pulmonary complications occur in half of patients with perioperative SARS-CoV-2 infection and are associated with high mortality. Thresholds for surgery during the COVID-19 pandemic should be higher than during normal practice, particularly in men aged 70 years and older. Consideration should be given for postponing non-urgent procedures and promoting non-operative treatment to delay or avoid the need for surgery. Funding: National Institute for Health Research (NIHR), Association of Coloproctology of Great Britain and Ireland, Bowel and Cancer Research, Bowel Disease Research Foundation, Association of Upper Gastrointestinal Surgeons, British Association of Surgical Oncology, British Gynaecological Cancer Society, European Society of Coloproctology, NIHR Academy, Sarcoma UK, Vascular Society for Great Britain and Ireland, and Yorkshire Cancer Research
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