Research on the structure of a new type of multi party bicycle system

at present, many people’s gathering bicycles suitable for scenic spots, parks and pedestrian streets are popular abroad, but they have not been introduced in China. Based on the analysis of foreign multi person bicycles, this paper improves the suspension device, steering device, transmission device, braking device and parking device of bicycle vehicles, so as to improve the ability of multi person gathering bicycles to adapt to diff erent road conditions and the safety, reliability and comfort in the process of riding. The research results are expected to provide reference for the development of multi-party bicycles in China

Topology control of VANET based on the multi-radio in vehicle and traffic flow in road network

Za pokretnu mrežu interneta (Vehicular Ad Hoc Networks, VANET), određivanje resursa, pravila industrije transporta i potreba održavanja, rad daje sigurnu i učinkovitu topologiju mobilnog interneta i dizajnira multi-radio čvorove u vozilu, a zatim izrađuje algoritam optimizacije kolonije mrava usmjeren virtualnoj sili kroz topologiju izvornog pristupa kako bi stvorio abstraktnu cestovnu mrežu. Na osnovu tih ideja, gradimo postupak umrežavanja i održavanja u pojedinim dijelovima. Konačno provjeravamo izvedivost i učinkovitost strategije kontrole topologije VANET-a zasnovanu na multi-radiju u vozilu i toku prometa na cestovnoj mreži kroz simulirani eksperiment.For mobile internet network (Vehicular Ad Hoc Networks, VANET), resources allocation, transportation industry regulations and service business requirements, this paper builds a stable and efficient mobile internet topology and designs multi-radio nodes in vehicle, and then raises the virtual-force oriented ant colony optimization algorithm through primal approach topology to abstract road network. Based on these ideas, we design the process of networking and maintenance in sections. Finally, we verify the feasibility and effectiveness of topology control strategy of VANET based on the multi-radio in vehicle and traffic flow in road network through the simulation experiment

Hybrid acoustic metamaterial as super absorber for broadband low-frequency sound

A hybrid acoustic metamaterial is proposed as a new class of sound absorber, which exhibits superior broadband low-frequency sound absorption as well as excellent mechanical stiffness/strength. Based on the honeycomb-corrugation hybrid core (H-C hybrid core), we introduce perforations on both top facesheet and corrugation, forming perforated honeycomb-corrugation hybrid (PHCH) to gain super broadband low-frequency sound absorption. Applying the theory of micro-perforated panel (MPP), we establish a theoretical method to calculate the sound absorption coefficient of this new kind of metamaterial. Perfect sound absorption is found at just a few hundreds hertz with two-octave 0.5 absorption bandwidth. To verify this model, a finite element model is developed to calculate the absorption coefficient and analyze the viscous-thermal energy dissipation. It is found that viscous energy dissipation at perforation regions dominates the total energy consumed. This new kind of acoustic metamaterials show promising engineering applications, which can serve as multiple functional materials with extraordinary low-frequency sound absorption, excellent stiffness/strength and impact energy absorption

Jun-APOE-LRP1 axis promotes tumor metastasis in colorectal cancer

Apolipoprotein E (apoE) has previously been reported to play vital roles in tumor progression. However, the impact of apoE on colorectal cancer (CRC) metastasis remains largely unexplored. This study aimed to investigate the role of apoE in CRC metastasis and to identify the transcription factor and receptor of apoE involved in regulation of CRC metastasis. Bioinformatic analyses were conducted to examine the expression pattern and prognosis of apolipoproteins. APOE-overexpressing cell lines were utilized to explore the effects of apoE on proliferation, migration and invasion of CRC cells. Additionally, the transcription factor and receptor of apoE were screened via bioinformatics, and further validated through knockdown experiments. We discovered that the mRNA levels of APOC1, APOC2, APOD and APOE were higher in lymphatic invasion group, and a higher apoE level indicated poorer overall survival and progression-free interval. In vitro studies demonstrated that APOE-overexpression did not affect proliferation but promoted the migration and invasion of CRC cells. We also reported that APOE-expression was modulated by the transcription factor Jun by activating the proximal promoter region of APOE, and APOE-overexpression reversed the metastasis suppression of JUN knockdown. Furthermore, bioinformatics analysis suggested an interaction between apoE and low-density lipoprotein receptor-related protein 1 (LRP1). LRP1 was highly expressed in both the lymphatic invasion group and the APOEHigh group. Additionally, we found that APOE-overexpression upregulated LRP1 protein levels, and LRP1 knockdown attenuated the metastasis-promoting function of APOE. Overall, our study suggests that the Jun-APOE-LRP1 axis contributes to tumor metastasis in CRC

The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens

Background The Critical Assessment of Functional Annotation (CAFA) is an ongoing, global, community-driven effort to evaluate and improve the computational annotation of protein function. Results Here, we report on the results of the third CAFA challenge, CAFA3, that featured an expanded analysis over the previous CAFA rounds, both in terms of volume of data analyzed and the types of analysis performed. In a novel and major new development, computational predictions and assessment goals drove some of the experimental assays, resulting in new functional annotations for more than 1000 genes. Specifically, we performed experimental whole-genome mutation screening in Candida albicans and Pseudomonas aureginosa genomes, which provided us with genome-wide experimental data for genes associated with biofilm formation and motility. We further performed targeted assays on selected genes in Drosophila melanogaster, which we suspected of being involved in long-term memory. Conclusion We conclude that while predictions of the molecular function and biological process annotations have slightly improved over time, those of the cellular component have not. Term-centric prediction of experimental annotations remains equally challenging; although the performance of the top methods is significantly better than the expectations set by baseline methods in C. albicans and D. melanogaster, it leaves considerable room and need for improvement. Finally, we report that the CAFA community now involves a broad range of participants with expertise in bioinformatics, biological experimentation, biocuration, and bio-ontologies, working together to improve functional annotation, computational function prediction, and our ability to manage big data in the era of large experimental screens.Peer reviewe

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens

BackgroundThe Critical Assessment of Functional Annotation (CAFA) is an ongoing, global, community-driven effort to evaluate and improve the computational annotation of protein function.ResultsHere, we report on the results of the third CAFA challenge, CAFA3, that featured an expanded analysis over the previous CAFA rounds, both in terms of volume of data analyzed and the types of analysis performed. In a novel and major new development, computational predictions and assessment goals drove some of the experimental assays, resulting in new functional annotations for more than 1000 genes. Specifically, we performed experimental whole-genome mutation screening in Candida albicans and Pseudomonas aureginosa genomes, which provided us with genome-wide experimental data for genes associated with biofilm formation and motility. We further performed targeted assays on selected genes in Drosophila melanogaster, which we suspected of being involved in long-term memory.ConclusionWe conclude that while predictions of the molecular function and biological process annotations have slightly improved over time, those of the cellular component have not. Term-centric prediction of experimental annotations remains equally challenging; although the performance of the top methods is significantly better than the expectations set by baseline methods in C. albicans and D. melanogaster, it leaves considerable room and need for improvement. Finally, we report that the CAFA community now involves a broad range of participants with expertise in bioinformatics, biological experimentation, biocuration, and bio-ontologies, working together to improve functional annotation, computational function prediction, and our ability to manage big data in the era of large experimental screens.</p

Construction de vecteurs et contrôle translationnel pour une thérapie génique basée sur le GM-CSF

La dégénérescence maculaire liée à l'âge (DMLA) est une neurodégénérescence de la rétine et représente la première cause de cécité chez les patients âgés de plus de 60 ans. Pour la forme sèche de la pathologie il n'existe toutefois pas de traitement à l'heure actuelle. Ici, une thérapie génique utilisant le système de transposition Sleeping Beauty (SB100X ) a été développée avec le gène d'intérêt (GM-CSF) comme agent neuroprotecteur pour traiter la DMLA sèche. GM-CSF a pu être introduit dans un vecteur et être multiplié. Le transfection des cellules ARPE-19 a démontré la vitalité des cellules transfectées. Une soft-agar assay a exclu la tumorigénicité du système SB100X utilisant un plasmide codant le PEDF neuroprotecteur. Bien que l'expression de la protéine recombinante n'ait pu être évaluée jusqu'à présent, la RCP a prouvé la transfection et intégration réussie du gène GM-CSF. Les résultats presents démontrent la faisabilité et sécurité de l'approche in vitro

Comparison of antibiotic-impregnated bone cement coverage versus vacuum sealing drainage in semi-open bone grafting using for tibial fracture with infected bone and soft tissue defect: a retrospective analysis

Abstract Objective To compare antibiotic-impregnated bone cement coverage (bone cement surface technique; BCS-T) versus vacuum sealing drainage (VSD) for tibial fracture with infected bone and soft tissue defect. Method This retrospective analysis compared the clinical outcomes in patients undergoing BCS-T (n = 16) versus VSD (n = 15) for tibial fracture with infected bone and soft tissue defect at the Third Hospital of Hebei Medical University from March 2014 to August 2019. For BCS-T group, osseous cavity was filled with autograft bone graft after debridement, and then the wound was covered with a 3-mm layer of bone cement impregnated with vancomycin and gentamycin. The dressing was changed every day in the first week, and every 2 ~ 3 days in the second week. For VSD group, a negative pressure of -150 ~ -350 mmHg was maintained, and the dressing was changed every 5–7 days. All patients received antibiotics treatment based on bacterial culture results for 2 weeks. Results The 2 groups did not differ in age, sex and key baseline characteristics, including type of Gustilo-Anderson classification, size of the bone and soft tissue defect, the percentage of primary debridement, bone transport, and the time from injury to bone grafting. The median follow-up was 18.9 months (range:12–40). The time to complete coverage of bone graft by granulation tissue was 21.2 (15.0–44.0) and 20.3 (15.0–24.0) days in the BCS-T and VSD groups, respectively (p = 0.412). The 2 groups also did not differ in wound healing time (3.3 (1.5–5.5) versus 3.2(1.5–6.5) months; p = 0.229) and bone defect healing time (5.4(3.0-9.6) versus 5.9(3.2–11.5) months; p = 0.402). However, the cost of covering material was significantly reduced in the BCS-T group (2071 ± 134 versus 5542 ± 905 yuan; p = 0.026). Paley functional classification at 12 months did not differ between the 2 groups (excellent in 87.5% versus 93.3% in the 2 groups; p = 0.306). Conclusion BCS-T could achieve clinical outcomes similar to VSD in patients receiving bone graft for tibial fracture with infected bone and soft tissue defect, but material cost was significantly reduced. Randomized controlled trials are needed to verify our finding

Tunable acoustic absorbers with periodical micro-perforations having varying pore shapes

Circular pores with sub-millimeter diameters have been widely used to construct micro-perforated panels (MPPs), the acoustical performance of which can be predicted well using the Maa theory (Ma