THE CHANGE OF ARCHITECTONICS OF AN EAR AS A FACTOR OF INCREASING THE PRODUCTIVITY OF WINTER RYE

The change of architectonics of plants helps to optimize an optical-crop biological structure and to form the new morphological features of plants designed to increase the yield of crops. The changes of architectonics of an ear plant of winter rye to improve a performance of culture are grounded theoretically. It is proved that polystaching is one of the possible ways to improve plant productivity. Ramification of a rod ear makes it possible to increase the number of ears in the inflorescence and flowers and seeds according to the plant. The results of monitoring of cultural plant populations of winter rye resulting in isolated samples of the branched structure of the ear are shown. Polystachyres form received in the creation of candidates for sterility fixers of rye varieties through a hybridization with domestic and foreign lines of hybrids. The selected sample 19–5 is analyzed by the major phenotypic characteristics. The studies found that an isolated polystachyres form has several agronomic traits, the main of which is higher than the grade-standard offering. Sample 19–5 should be used in breeding research as a donor of polystaching, short-stemming, increasing the length of the ear, number of grains in the ear and ear weight and grain, respectively, from the plant

Study of the electron-phonon interaction in metal diborides MeB_2 (Me=Zr, Nb, Ta, Mg) by point-contact spectroscopy

We review investigations of the electron-phonon interaction (EPI) in metal diborides MeB_2 (Me=Zr, Nb, Ta, Mg) by point-contact (PC) spectroscopy. For transition metal compounds the PC EPI functions were recovered and EPI parameter $\lambda\lesssim 0.1$ were estimated. The data are consistent with the measured surface phonon dispersion curves. The low $\lambda$ value questions some reports about superconductivity in these compounds. Contrary, EPI in superconducting MgB_2 films manifests also in the PC spectra itself by virtue of an elastic EPI contribution to the excess current determined by the energy dependence of the superconducting order parameter. To analyse the phonon features in the PC spectra of MgB$_2$ a two-band model is exploited and the proximity effect in the {\bf k}-space is suggested.Comment: 6 pages, 6 figs., prepared for proceedings of conference "Modern Problem in Superconductivity", Sept. 2002, Yalta, Ukrain

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

No abstract available

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present