226 research outputs found
Single seed-based high-throughput genotyping and rapid generation advancement for accelerated groundnut genetics and breeding research
The groundnut breeding program at International Crops Research Institute for the Semi-Arid Tropics routinely performs marker-based early generation selection (MEGS) in thousands of segregating populations. The existing MEGS includes planting of segregating populations in fields or glasshouses, label tagging, and sample collection using leaf-punch from 20â25 day old plants followed by genotyping with 10 single nucleotide polymorphisms based early generation selection marker panels in a high throughput genotyping (HTPG) platform. The entire process is laborious, time consuming, and costly. Therefore, in order to save the time of the breeder and to reduce the cost during MEGS, we optimized a single seed chipping (SSC) process based MEGS protocol and deployed on large scale by genotyping >3000 samples from ongoing groundnut breeding program. In SSC-based MEGS, we used a small portion of cotyledon by slicing-off the posterior end of the single seed and transferred to the 96-deep well plate for DNA isolation and genotyping at HTPG platform. The chipped seeds were placed in 96-well seed-box in the same order of 96-well DNA sampling plate to enable tracking back to the selected individual seed. A high germination rate of 95â99% from the chipped seeds indicated that slicing of seeds from posterior end does not significantly affect germination percentage. In addition, we could successfully advance 3.5 generations in a year using a low-cost rapid generation turnover glass-house facility as compared to routine practice of two generations in field conditions. The integration of SSC based genotyping and rapid generation advancement (RGA) could significantly reduce the operational requirement of person-hours and expenses, and save a period of 6â8 months in groundnut genetics and breeding research
Dexamethasone and supportive care with or without whole brain radiotherapy in treating patients with non-small cell lung cancer with brain metastases unsuitable for resection or stereotactic radiotherapy (QUARTZ): results from a phase 3, non-inferiority, randomised trial
Background
Whole brain radiotherapy (WBRT) and dexamethasone are widely used to treat brain metastases from
non-small cell lung cancer (NSCLC), although there have been no randomised clinical trials showing that WBRT
improves either quality of life or overall survival. Even after treatment with WBRT, the prognosis of this patient group
is poor. We aimed to establish whether WBRT could be omitted without a signifi cant eff ect on survival or quality of life.
Methods
The Quality of Life after Treatment for Brain Metastases (QUARTZ) study is a non-inferiority, phase 3
randomised trial done at 69 UK and three Australian centres. NSCLC patients with brain metastases unsuitable for
surgical resection or stereotactic radiotherapy were randomly assigned (1:1) to optimal supportive care (OSC)
including dexamethasone plus WBRT (20 Gy in five daily fractions) or OSC alone (including dexamethasone). The
dose of dexamethasone was determined by the patientsâ symptoms and titrated downwards if symptoms improved.
Allocation to treatment group was done by a phone call from the hospital to the Medical Research Council Clinical
Trials Unit at University College London using a minimisation programme with a random element and stratifi cation
by centre, Karnofsky Performance Status (KPS), gender, status of brain metastases, and the status of primary lung
cancer. The primary outcome measure was quality-adjusted life-years (QALYs). QALYs were generated from overall
survival and patientsâ weekly completion of the EQ-5D questionnaire. Treatment with OSC alone was considered noninferior
if it was no more than 7 QALY days worse than treatment with WBRT plus OSC, which required 534 patients
(80% power, 5% [one-sided] signifi cance level). Analysis was done by intention to treat for all randomly assigned
patients. The trial is registered with ISRCTN, number ISRCTN3826061.
Findings
Between March 2, 2007, and Aug 29, 2014, 538 patients were recruited from 69 UK and three Australian
centres, and were randomly assigned to receive either OSC plus WBRT (269) or OSC alone (269). Baseline characteristics
were balanced between groups, and the median age of participants was 66 years (range 38â85). Signifi cantly more
episodes of drowsiness, hair loss, nausea, and dry or itchy scalp were reported while patients were receiving WBRT,
although there was no evidence of a difference in the rate of serious adverse events between the two groups. There was
no evidence of a diff erence in overall survival (hazard ratio 1·06, 95% CI 0·90â1·26), overall quality of life, or
dexamethasone use between the two groups. The diff erence between the mean QALYs was 4·7 days (46·4 QALY days
for the OSC plus WBRT group vs 41·7 QALY days for the OSC group), with two-sided 90% CI of â12·7 to 3·3.
Interpretation
Although the primary outcome measure result includes the prespecifi ed non-inferiority margin, the
combination of the small diff erence in QALYs and the absence of a diff erence in survival and quality of life between
the two groups suggests that WBRT provides little additional clinically signifi cant benefi t for this patient group
Formation of dense partonic matter in relativistic nucleus-nucleus collisions at RHIC: Experimental evaluation by the PHENIX collaboration
Extensive experimental data from high-energy nucleus-nucleus collisions were
recorded using the PHENIX detector at the Relativistic Heavy Ion Collider
(RHIC). The comprehensive set of measurements from the first three years of
RHIC operation includes charged particle multiplicities, transverse energy,
yield ratios and spectra of identified hadrons in a wide range of transverse
momenta (p_T), elliptic flow, two-particle correlations, non-statistical
fluctuations, and suppression of particle production at high p_T. The results
are examined with an emphasis on implications for the formation of a new state
of dense matter. We find that the state of matter created at RHIC cannot be
described in terms of ordinary color neutral hadrons.Comment: 510 authors, 127 pages text, 56 figures, 1 tables, LaTeX. Submitted
to Nuclear Physics A as a regular article; v3 has minor changes in response
to referee comments. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
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The new Victorians? Celebrity charity and the demise of the welfare state
This article asks whether the expansion of celebrity involvement in charitable and humanitarian issues in Northern Europe and the US might be a comparable historical phenomenon with the philanthropic endeavours of prominent nineteenth-century persons. The article notes that the conspicuous nature of star philanthropy in both Victorian times and the present is fairly dramatic in comparison with that of the mid twentieth century, when the welfare state and the New Deal were at their peak: a welfare-oriented era which, to some, now increasingly looks like a âhistorical blipâ. It asks whether the rise of contemporary celebrity involvement in charity can therefore be explained in terms of the contemporary political conjuncture, inasmuch as celebrities could be understood as individuals with large amounts of private capital seeking to intervene in â and gain forms of power through â involvement in humanitarian and charitable causes that might have formerly been the job of the state. Can celebrity involvement in charity be explained in these terms? Does the marriage of celebrity and charity today take a neoliberal form, one that parallels the liberal form of nineteenth-century interventions, bequests and donations? What might the key differences between forms of spectacular âphilanthrocapitalismâ in these eras (particularly the contemporary insistence on the confessional and intimate modes of address) reveal about its workings, its internal traditions and about the specificity of our own age? This article draws on contemporary media discourse, debate in the voluntary sector, historical scholarship and Foucaultâs distinctions between liberalism and neoliberalism to argue that whereas âcelanthropyâ in the Victorian period eventually came to contribute to the welfare state, today it is more involved in privatising and dismantling it
Saturation of azimuthal anisotropy in Au + Au collisions at sqrt(s_NN) = 62 - 200 GeV
New measurements are presented for charged hadron azimuthal correlations at
mid-rapidity in Au+Au collisions at sqrt(s_NN) = 62.4 and 200 GeV. They are
compared to earlier measurements obtained at sqrt(s_NN) = 130 GeV and in Pb+Pb
collisions at sqrt(s_NN) = 17.2 GeV. Sizeable anisotropies are observed with
centrality and transverse momentum (p_T) dependence characteristic of elliptic
flow (v_2). For a broad range of centralities, the observed magnitudes and
trends of the differential anisotropy, v_2(p_T), change very little over the
collision energy range sqrt(s_NN) = 62-200 GeV, indicating saturation of the
excitation function for v_2 at these energies. Such a saturation may be
indicative of the dominance of a very soft equation of state for sqrt(s_NN) =
62-200 GeV.Comment: 432 authors, 7 pages text, 4 figures, REVTeX4. To be submitted to
Physical Review Letters. Plain text data tables for the points plotted in
figures for this and previous PHENIX publications are (or will be) publicly
available at http://www.phenix.bnl.gov/papers.htm
An Observational Overview of Solar Flares
We present an overview of solar flares and associated phenomena, drawing upon
a wide range of observational data primarily from the RHESSI era. Following an
introductory discussion and overview of the status of observational
capabilities, the article is split into topical sections which deal with
different areas of flare phenomena (footpoints and ribbons, coronal sources,
relationship to coronal mass ejections) and their interconnections. We also
discuss flare soft X-ray spectroscopy and the energetics of the process. The
emphasis is to describe the observations from multiple points of view, while
bearing in mind the models that link them to each other and to theory. The
present theoretical and observational understanding of solar flares is far from
complete, so we conclude with a brief discussion of models, and a list of
missing but important observations.Comment: This is an article for a monograph on the physics of solar flares,
inspired by RHESSI observations. The individual articles are to appear in
Space Science Reviews (2011
Ï production in pâPb collisions at âsNN=8.16 TeV
Ï production in pâPb interactions is studied at the centre-of-mass energy per nucleonânucleon collision âsNN = 8.16 TeV with the ALICE detector at the CERN LHC. The measurement is performed reconstructing bottomonium resonances via their dimuon decay channel, in the centre-of-mass rapidity intervals 2.03 < ycms < 3.53 and â4.46 < ycms < â2.96, down to zero transverse momentum. In this work, results on the Ï(1S) production cross section as a function of rapidity and transverse momentum are presented. The corresponding nuclear modification factor shows a suppression of the Ï(1S) yields with respect to pp collisions, both at forward and backward rapidity. This suppression is stronger in the low transverse momentum region and shows no significant dependence on the centrality of the interactions. Furthermore, the Ï(2S) nuclear modification factor is evaluated, suggesting a suppression similar to that of the Ï(1S). A first measurement of the Ï(3S) has also been performed. Finally, results are compared with previous ALICE measurements in pâPb collisions at âsNN = 5.02 TeV and with theoretical calculations.publishedVersio
(Anti-)deuteron production in pp collisions at 1as=13TeV
The study of (anti-)deuteron production in pp collisions has proven to be a powerful tool to investigate the formation mechanism of loosely bound states in high-energy hadronic collisions. In this paper the production of (anti-)deuterons is studied as a function of the charged particle multiplicity in inelastic pp collisions at s=13 TeV using the ALICE experiment. Thanks to the large number of accumulated minimum bias events, it has been possible to measure (anti-)deuteron production in pp collisions up to the same charged particle multiplicity (d Nch/ d \u3b7 3c 26) as measured in p\u2013Pb collisions at similar centre-of-mass energies. Within the uncertainties, the deuteron yield in pp collisions resembles the one in p\u2013Pb interactions, suggesting a common formation mechanism behind the production of light nuclei in hadronic interactions. In this context the measurements are compared with the expectations of coalescence and statistical hadronisation models (SHM)
Multiplicity dependence of inclusive J/psi production at midrapidity in pp collisions at root s=13 TeV
Measurements of the inclusive J/psi yield as a function of charged-particle pseudorapidity density dN(ch)/d eta in pp collisions at root s = 13 TeV with ALICE at the LHC are reported. The J/psi meson yield is measured at midrapidity (vertical bar y vertical bar <0.9) in the dielectron channel, for events selected based on the charged-particle multiplicity at midrapidity (vertical bar eta vertical bar <1) and at forward rapidity (-3.7 <eta <-1.7 and 2.8 <eta <5.1); both observables are normalized to their corresponding averages in minimum bias events. The increase of the normalized J/psi yield with normalized dN(ch)/d eta is significantly stronger than linear and dependent on the transverse momentum. The data are compared to theoretical predictions, which describe the observed trends well, albeit not always quantitatively. (C) 2020 European Organization for Nuclear Research. Published by Elsevier B.V.Peer reviewe
Measurement of jet suppression in central Pb-Pb collisions at root s(NN)=2.76 TeV
The transverse momentum(p(T)) spectrum and nuclear modification factor (R-AA) of reconstructed jets in 0-10% and 10-30% central Pb-Pb collisions at root s(NN) = 2.76 TeV were measured. Jets were reconstructed using the anti-k(T) jet algorithm with a resolution parameter of R = 0.2 from charged and neutral particles, utilizing the ALICE tracking detectors and Electromagnetic Calorimeter (EMCal). The jet p(T) spectra are reported in the pseudorapidity interval of \eta(jet)\ 5 GeV/c to suppress jets constructed from the combinatorial background in Pb-Pb collisions. The leading charged particle requirement applied to jet spectra both in pp and Pb-Pb collisions had a negligible effect on the R-AA. The nuclear modification factor R-AA was found to be 0.28 +/- 0.04 in 0-10% and 0.35 +/- 0.04 in 10-30% collisions, independent of p(T), jet within the uncertainties of the measurement. The observed suppression is in fair agreement with expectations from two model calculations with different approaches to jet quenching. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V.Peer reviewe
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