Management of an emerging pest, Tetranychus urticae Koch (Acari: Tetranychidae), with pesticides in eastern Ethiopia

Outbreak of two-spotted spider mite, Tetranychus urticae Koch, happened on potato ( Solanum tuberosum L.) in eastern Ethiopia in 2014 and effective pesticides as part of IPM practice were sought from greenhouse and laboratory experiments at Haramaya University, Ethiopia. The objective of the study was to identify pesticides with the highest efficacy against the mite. Potatoes infested with mites were used in the efficacy trial. Treatments, 9 pesticides and a control, were applied 2 times at weekly intervals. Mites per leaf before and after each spray were counted. Promising pesticides were further screened with leaf-disc spray, and dip methods. Adult mortality was recorded after 24 and 48 hours. Chlorantrniliprole + Lambda-cyhalothrin, Amitraz, Profenofos, Profenofos \u201cQ\u201d 720 g l-1, and Paraffin oil showed superior efficacy on eggs and mobile stages; though Paraffin\u2019s efficacy became clear 14 days later. Paraffin had the lowest mortality in leaf-disc sprays, but better mortality in the leaf-disc dip. This was attributed to complete wetting by the latter. Chlorantrniliprole + lambda-cyhalothrin, Profenofos, Profenofos \u201cQ\u201d and Paraffin were identified as promising pesticides for the two spotted spider mite management on potato.L\u2019explosion de t\ue9tranyque \ue0 deux points, Tetranychus urticae Koch, s\u2019est produite sur la patate ( Solanum tuberosum L.) dans l\u2019Est de l\u2019Ethiopie en 2014 et des pesticides effectifs comme une partie de de pratique IPM a \ue9t\ue9 recherch\ue9e \ue0 partir des exp\ue9rimentations sous serre et au laboratoire \ue0 l\u2019Universit\ue9 de Haramaya, Ethiopie. L\u2019objectif de l\u2019\ue9tude \ue9tait d\u2019identifier les pesticides avec de tr\ue8s grande efficacit\ue9 contre le t\ue9tranyque. Les patates infest\ue9es avec les t\ue9tranyques ont \ue9t\ue9 utilis\ue9es dans un essai d\u2019efficacit\ue9. Les traitements, 9 pesticides et un contr\uf4le, \ue9taient appliqu\ue9s deux fois \ue0 des intervalles d\u2019une semaine. Les t\ue9tranyques par feuille ont \ue9t\ue9 compt\ue9s avant et apr\ue8s chaque traitement. Des pesticidesprometteurs ont \ue9t\ue9 \ue9galement\ue9valu\ue9s avec le pulv\ue9riseur \ue0 disque sur les feuilles, et les m\ue9thodes d\u2019immersion. La mortalit\ue9 de l\u2019adulte\ue9tait evalu\ue9e apr\ue8s 24 et 48 heures. Chlorantrniliprole + Lambda-cyhalothrin, Amitraz, Profenofos, Profenofos \u201cQ\u201d 720 g l-1, et l\u2019huile du Paraffine ont montr\ue9 une efficacit\ue9 sup\ue9rieure sur les \u153ufs, stages mobiles\ua0; quoique l\u2019efficacit\ue9 de paraffine est devenue claire \ue0 14 jours apr\ue8s. Paraffine a eu la plus faible mortalit\ue9 avec le pulv\ue9riseur \ue0 disque sur la feuille, mais meilleure mortalit\ue9 avec l\u2019immersion de la feuille. Ceci a \ue9t\ue9 attribu\ue9 \ue0 la mouillure compl\ue8te de la deuxi\ue8me m\ue9thode. Chlorantrniliprole + lambda-cyhalothrin, Profenofos, profenofos \u201cQ\u201d et Paraffine ont \ue9t\ue9 identifi\ue9s comme pesticide prometteurs, pour la gestion du t\ue9tranyque \ue0 deux points sur la tomate

Evaluation of the protective efficacy of Salmonella Gallinarum 9R strain vaccine against Salmonella strains isolated from cases suspected of salmonellosis outbreaks in poultry farms in central Ethiopia

Salmonellosis is one of the most important bacterial diseases of poultry causing heavy economic losses. Though it can be prevented through vaccination, regular monitoring of the protective efficacy of the vaccine against field outbreaks is essential since antigenically dissimilar serovars may evolve compromising the efficacy of the vaccine. This study was, therefore, carried out to evaluate the protective efficacy of the currently used attenuated Salmonella Gallinarum 9R strain vaccine against experimental challenge with field isolates of Salmonella strains obtained from disease outbreaks. Three Salmonella serovars viz Salmonella Gallinarum, Salmonella Pullorum and Salmonella Enteritidis isolated and identified (phenotypically and molecularly) from outbreaks suspected of salmonellosis were used for the challenge experiment. A total of 90 chickens randomly divided into 2 categories (vaccinated and non-vaccinated control) each comprising 45 birds were used for protective efficacy study. Each of these categories were further subdivided and randomly assigned into 3 groups each comprising 15 birds for challenge with each of the three field Salmonella serovars. Chickens were vaccinated subcutaneously with live attenuated Salmonella Gallinarum 9R vaccine containing 2x107 CFU per dose. Each group in both vaccinated and non vaccinated category were challenged with the three field serovars (one strain per group) through oral administration of 1 ml of bacterial suspension containing 5×107organisms. Post challenge follow-up showed no mortality in all vaccinated groups challenged with each of the three serovars while a mortality of 53.3% (N=8), 13.3% (N=2) and 0% was observed in S. Gallinarum, S. Pullorum and S. Enteritidis challenged nonvaccinated groups, respectively. None of the challenge Salmonella strains were  recovered from liver and spleen of the vaccinated birds two weeks after challenge. In conclusion, the currently used attenuated S. Gallinarum 9R strain vaccine against fowl typhoid can effectively confer protection not only against field strains of Salmonella Gallinarum, but also cross-protection to S. Pullorum and S. Enteritidis involved in causing poultry salmonellosis outbreaks in Ethiopia.Keywords: Central Ethiopia; Poultry; Salmonellosis; Vaccine efficac

Global, regional, and national burden of chronic kidney disease, 1990–2017 : a systematic analysis for the Global Burden of Disease Study 2017

Background Health system planning requires careful assessment of chronic kidney disease (CKD) epidemiology, but data for morbidity and mortality of this disease are scarce or non-existent in many countries. We estimated the global, regional, and national burden of CKD, as well as the burden of cardiovascular disease and gout attributable to impaired kidney function, for the Global Burden of Diseases, Injuries, and Risk Factors Study 2017. We use the term CKD to refer to the morbidity and mortality that can be directly attributed to all stages of CKD, and we use the term impaired kidney function to refer to the additional risk of CKD from cardiovascular disease and gout. Methods The main data sources we used were published literature, vital registration systems, end-stage kidney disease registries, and household surveys. Estimates of CKD burden were produced using a Cause of Death Ensemble model and a Bayesian meta-regression analytical tool, and included incidence, prevalence, years lived with disability, mortality, years of life lost, and disability-adjusted life-years (DALYs). A comparative risk assessment approach was used to estimate the proportion of cardiovascular diseases and gout burden attributable to impaired kidney function. Findings Globally, in 2017, 1·2 million (95% uncertainty interval [UI] 1·2 to 1·3) people died from CKD. The global all-age mortality rate from CKD increased 41·5% (95% UI 35·2 to 46·5) between 1990 and 2017, although there was no significant change in the age-standardised mortality rate (2·8%, −1·5 to 6·3). In 2017, 697·5 million (95% UI 649·2 to 752·0) cases of all-stage CKD were recorded, for a global prevalence of 9·1% (8·5 to 9·8). The global all-age prevalence of CKD increased 29·3% (95% UI 26·4 to 32·6) since 1990, whereas the age-standardised prevalence remained stable (1·2%, −1·1 to 3·5). CKD resulted in 35·8 million (95% UI 33·7 to 38·0) DALYs in 2017, with diabetic nephropathy accounting for almost a third of DALYs. Most of the burden of CKD was concentrated in the three lowest quintiles of Socio-demographic Index (SDI). In several regions, particularly Oceania, sub-Saharan Africa, and Latin America, the burden of CKD was much higher than expected for the level of development, whereas the disease burden in western, eastern, and central sub-Saharan Africa, east Asia, south Asia, central and eastern Europe, Australasia, and western Europe was lower than expected. 1·4 million (95% UI 1·2 to 1·6) cardiovascular disease-related deaths and 25·3 million (22·2 to 28·9) cardiovascular disease DALYs were attributable to impaired kidney function. Interpretation Kidney disease has a major effect on global health, both as a direct cause of global morbidity and mortality and as an important risk factor for cardiovascular disease. CKD is largely preventable and treatable and deserves greater attention in global health policy decision making, particularly in locations with low and middle SDI

The global, regional, and national burden of cirrhosis by cause in 195 countries and territories, 1990-2017 : a systematic analysis for the Global Burden of Disease Study 2017

Background Cirrhosis and other chronic liver diseases (collectively referred to as cirrhosis in this paper) are a major cause of morbidity and mortality globally, although the burden and underlying causes differ across locations and demographic groups. We report on results from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2017 on the burden of cirrhosis and its trends since 1990, by cause, sex, and age, for 195 countries and territories. Methods We used data from vital registrations, vital registration samples, and verbal autopsies to estimate mortality. We modelled prevalence of total, compensated, and decompensated cirrhosis on the basis of hospital and claims data. Disability-adjusted life-years (DALYs) were calculated as the sum of years of life lost due to premature death and years lived with disability. Estimates are presented as numbers and age-standardised or age-specific rates per 100 000 population, with 95% uncertainty intervals (UIs). All estimates are presented for five causes of cirrhosis: hepatitis B, hepatitis C, alcohol-related liver disease, non-alcoholic steatohepatitis (NASH), and other causes. We compared mortality, prevalence, and DALY estimates with those expected according to the Socio-demographic Index (SDI) as a proxy for the development status of regions and countries. Findings In 2017, cirrhosis caused more than 1.32 million (95% UI 1.27-1.45) deaths (440000 [416 000-518 000; 33.3%] in females and 883 000 [838 000-967 000; 66.7%] in males) globally, compared with less than 899 000 (829 000-948 000) deaths in 1990. Deaths due to cirrhosis constituted 2.4% (2.3-2.6) of total deaths globally in 2017 compared with 1.9% (1.8-2.0) in 1990. Despite an increase in the number of deaths, the age-standardised death rate decreased from 21.0 (19.2-22.3) per 100 000 population in 1990 to 16.5 (15.8-18-1) per 100 000 population in 2017. Sub-Saharan Africa had the highest age-standardised death rate among GBD super-regions for all years of the study period (32.2 [25.8-38.6] deaths per 100 000 population in 2017), and the high-income super-region had the lowest (10.1 [9.8-10-5] deaths per 100 000 population in 2017). The age-standardised death rate decreased or remained constant from 1990 to 2017 in all GBD regions except eastern Europe and central Asia, where the age-standardised death rate increased, primarily due to increases in alcohol-related liver disease prevalence. At the national level, the age-standardised death rate of cirrhosis was lowest in Singapore in 2017 (3.7 [3.3-4.0] per 100 000 in 2017) and highest in Egypt in all years since 1990 (103.3 [64.4-133.4] per 100 000 in 2017). There were 10.6 million (10.3-10.9) prevalent cases of decompensated cirrhosis and 112 million (107-119) prevalent cases of compensated cirrhosis globally in 2017. There was a significant increase in age-standardised prevalence rate of decompensated cirrhosis between 1990 and 2017. Cirrhosis caused by NASH had a steady age-standardised death rate throughout the study period, whereas the other four causes showed declines in age-standardised death rate. The age-standardised prevalence of compensated and decompensated cirrhosis due to NASH increased more than for any other cause of cirrhosis (by 33.2% for compensated cirrhosis and 54.8% for decompensated cirrhosis) over the study period. From 1990 to 2017, the number of prevalent cases snore than doubled for compensated cirrhosis due to NASH and more than tripled for decompensated cirrhosis due to NASH. In 2017, age-standardised death and DALY rates were lower among countries and territories with higher SDI. Interpretation Cirrhosis imposes a substantial health burden on many countries and this burden has increased at the global level since 1990, partly due to population growth and ageing. Although the age-standardised death and DALY rates of cirrhosis decreased from 1990 to 2017, numbers of deaths and DALYs and the proportion of all global deaths due to cirrhosis increased. Despite the availability of effective interventions for the prevention and treatment of hepatitis B and C, they were still the main causes of cirrhosis burden worldwide, particularly in low-income countries. The impact of hepatitis B and C is expected to be attenuated and overtaken by that of NASH in the near future. Cost-effective interventions are required to continue the prevention and treatment of viral hepatitis, and to achieve early diagnosis and prevention of cirrhosis due to alcohol-related liver disease and NASH. Copyright (C) 2020 The Author(s). Published by Elsevier Ltd.Peer reviewe

Global injury morbidity and mortality from 1990 to 2017 : results from the Global Burden of Disease Study 2017

Correction:Background Past research in population health trends has shown that injuries form a substantial burden of population health loss. Regular updates to injury burden assessments are critical. We report Global Burden of Disease (GBD) 2017 Study estimates on morbidity and mortality for all injuries. Methods We reviewed results for injuries from the GBD 2017 study. GBD 2017 measured injury-specific mortality and years of life lost (YLLs) using the Cause of Death Ensemble model. To measure non-fatal injuries, GBD 2017 modelled injury-specific incidence and converted this to prevalence and years lived with disability (YLDs). YLLs and YLDs were summed to calculate disability-adjusted life years (DALYs). Findings In 1990, there were 4 260 493 (4 085 700 to 4 396 138) injury deaths, which increased to 4 484 722 (4 332 010 to 4 585 554) deaths in 2017, while age-standardised mortality decreased from 1079 (1073 to 1086) to 738 (730 to 745) per 100 000. In 1990, there were 354 064 302 (95% uncertainty interval: 338 174 876 to 371 610 802) new cases of injury globally, which increased to 520 710 288 (493 430 247 to 547 988 635) new cases in 2017. During this time, age-standardised incidence decreased non-significantly from 6824 (6534 to 7147) to 6763 (6412 to 7118) per 100 000. Between 1990 and 2017, age-standardised DALYs decreased from 4947 (4655 to 5233) per 100 000 to 3267 (3058 to 3505). Interpretation Injuries are an important cause of health loss globally, though mortality has declined between 1990 and 2017. Future research in injury burden should focus on prevention in high-burden populations, improving data collection and ensuring access to medical care.Peer reviewe

Estimating global injuries morbidity and mortality : methods and data used in the Global Burden of Disease 2017 study

Background While there is a long history of measuring death and disability from injuries, modern research methods must account for the wide spectrum of disability that can occur in an injury, and must provide estimates with sufficient demographic, geographical and temporal detail to be useful for policy makers. The Global Burden of Disease (GBD) 2017 study used methods to provide highly detailed estimates of global injury burden that meet these criteria. Methods In this study, we report and discuss the methods used in GBD 2017 for injury morbidity and mortality burden estimation. In summary, these methods included estimating cause-specific mortality for every cause of injury, and then estimating incidence for every cause of injury. Non-fatal disability for each cause is then calculated based on the probabilities of suffering from different types of bodily injury experienced. Results GBD 2017 produced morbidity and mortality estimates for 38 causes of injury. Estimates were produced in terms of incidence, prevalence, years lived with disability, cause-specific mortality, years of life lost and disability-adjusted life-years for a 28-year period for 22 age groups, 195 countries and both sexes. Conclusions GBD 2017 demonstrated a complex and sophisticated series of analytical steps using the largest known database of morbidity and mortality data on injuries. GBD 2017 results should be used to help inform injury prevention policy making and resource allocation. We also identify important avenues for improving injury burden estimation in the future.Peer reviewe

Mapping local patterns of childhood overweight and wasting in low- and middle-income countries between 2000 and 2017

A double burden of malnutrition occurs when individuals, household members or communities experience both undernutrition and overweight. Here, we show geospatial estimates of overweight and wasting prevalence among children under 5 years of age in 105 low- and middle-income countries (LMICs) from 2000 to 2017 and aggregate these to policy-relevant administrative units. Wasting decreased overall across LMICs between 2000 and 2017, from 8.4% (62.3 (55.1–70.8) million) to 6.4% (58.3 (47.6–70.7) million), but is predicted to remain above the World Health Organization’s Global Nutrition Target of <5% in over half of LMICs by 2025. Prevalence of overweight increased from 5.2% (30 (22.8–38.5) million) in 2000 to 6.0% (55.5 (44.8–67.9) million) children aged under 5 years in 2017. Areas most affected by double burden of malnutrition were located in Indonesia, Thailand, southeastern China, Botswana, Cameroon and central Nigeria. Our estimates provide a new perspective to researchers, policy makers and public health agencies in their efforts to address this global childhood syndemic

Association Mapping to Identify Sclerotinia sclerotiorum Genomic Regions Affecting Aggressiveness of Lesion Formation on Sunflower Stems

Sclerotinia sclerotiorum is one of the most destructive pathogens of sunflower in the United States and worldwide. Distinctive symptoms include mid-stalk rot, basal stalk rot and head rot from subsequent infection of the sunflower head. This fungal pathogen has a remarkably broad host range of over 400 dicot plants. However, little is currently known about the virulence strategies that allow S. sclerotiorum to successfully infect a wide range of plant hosts. The goal of our project was to identify S. sclerotiorum virulence determinants and effectors that contribute to disease development on sunflower. We evaluated a diverse collection of 232 S. sclerotiorum isolates for aggressiveness of mid-stalk lesion formation on two sunflower inbred lines. In addition, we performed genotyping-by-sequencing on 191 isolates to identify genetic markers for genome-wide association mapping to identify candidate genes associated with aggressiveness. A total of eight loci associated with variation in aggressiveness were identified

Invitro antibacterial activity of bark, leaf and root extracts of combretum molle plant against streptococcus equi isolated from clinical cases of strangles in donkeys and horses

Abstract Background Effective therapy for many infections is becoming difficult due to the evolutionary development of drug resistance, and hence, the development of alternative treatment options mainly from herbs is crucial. The objective of this study was to investigate the antibacterial effects of ethanol extracts of stem bark, leaves and roots of Combretum molle against Streptococcus equi isolated from clinical cases of strangles using in vitro tests. Methods Plant extraction was performed using a maceration technique with 80% ethanol. The mean zone of inhibition was determined using the agar well diffusion method. Six serial dilutions with different concentrations (10%, 5%, 2.5%, 1.25%, 0.625% and 0.3125%) of each plant extract were prepared using dimethyl sulfoxide (DMSO). A modified agar microdilution method was used to determine the minimum inhibitory concentration (MICs) of the extracts. Results The results revealed that all plant extracts showed significant antibacterial activity. The root extract showed the best antibacterial effect compared to the others at all concentrations, with MZI values of 27.5, 23.225, 20.5, 17.9, 15.65 and 12.25 for the respective concentrations mentioned above and an MIC of 250 µg/ml. It was followed by the stem bark extract, which had MZI values of 24.67, 22.35, 18.225, 16.175, 11.125 and 8.2 millimeters and an MIC of 375 µg/ml. The leaf extract also had significant activity, with MZI values of 20.175, 18.25, 15.7, 13.125, 9.4 and 6.75 in millimeters and an MIC of 500 µg/ml. There was a direct relationship between the concentrations of the plant extracts and the level of inhibition. Conclusion The test plant extracts were compared with the conventional antibiotic penicillin G, and the results indicated that the parts of the test plant have significant antibacterial activity, which may support traditional claims and could be candidates for alternative drug discoveries

Machine Learning Analysis of Hyperspectral Images of Damaged Wheat Kernels

Fusarium head blight (FHB) is a disease of small grains caused by the fungus Fusarium graminearum. In this study, we explored the use of hyperspectral imaging (HSI) to evaluate the damage caused by FHB in wheat kernels. We evaluated the use of HSI for disease classification and correlated the damage with the mycotoxin deoxynivalenol (DON) content. Computational analyses were carried out to determine which machine learning methods had the best accuracy to classify different levels of damage in wheat kernel samples. The classes of samples were based on the DON content obtained from Gas Chromatography–Mass Spectrometry (GC-MS). We found that G-Boost, an ensemble method, showed the best performance with 97% accuracy in classifying wheat kernels into different severity levels. Mask R-CNN, an instance segmentation method, was used to segment the wheat kernels from HSI data. The regions of interest (ROIs) obtained from Mask R-CNN achieved a high mAP of 0.97. The results from Mask R-CNN, when combined with the classification method, were able to correlate HSI data with the DON concentration in small grains with an R2 of 0.75. Our results show the potential of HSI to quantify DON in wheat kernels in commercial settings such as elevators or mills