90 research outputs found

    Stirring Unmagnetized Plasma

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    A new concept for spinning unmagnetized plasma is demonstrated experimentally. Plasma is confined by an axisymmetric multi-cusp magnetic field and biased cathodes are used to drive currents and impart a torque in the magnetized edge. Measurements show that flow viscously couples momentum from the magnetized edge (where the plasma viscosity is small) into the unmagnetized core (where the viscosity is large) and that the core rotates as a solid body. To be effective, collisional viscosity must overcome the ion-neutral drag due to charge exchange collisions

    Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)1

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    Premise of the study: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami?s urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae). Methods and Results: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (Ho) of 0.314 and average expected heterozygosity (He) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average Ho of 0.35 and an average He of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average Ho of 0.257 and an average He of 0.272. Conclusions: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project

    Impact of COVID-19 pandemic on cardiometabolic patients without SARS CoV-2 infection in Latin America

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    A cross-sectional survey including 38 questions about demography, clinical condition, changes in health habits, and medical treatments for cardiometabolic patients in outpatient follow-up was conducted. From June 15 to July 15, 2020, a total of 13 Latin-American countries participated in enrolling patients. These countries were divided into 3 geographic regions: Region 1 including North, Central, and Caribbean Regions (NCCR), Region 2 including the Andean Region (AR), and Region 3 including the Southern Cone Region (SCR). 4.216 patients were analyzed, resulting in a coefficient of 33.82%, 32.23%, and 33.94% for NCCR, AR, and SCR, respectively. Significant differences were found between the AR, SCR, and NCCR regions. The analysis of habitual medication usage showed that discontinued use of medication was more present in AR, reaching almost 30% (p < 0.001). The main finding of this study was the negative impact that restrictive measures have on adherence to medications and physical activity: Rs = 0.84 (p = 0.0003) and Rs = 0.61 (p = 0.0032), respectively. AR was the most vulnerable region. Restrictive quarantine measures imposed by the different countries showed a positive correlation with medication discontinuation and a negative correlation with physical activity levels in patients analyzed. These findings characterize the impact of the consequences left by this pandemic. Undoubtedly, restrictive measures have been and will continue to have reverberating negative effects in most Latin-American countries.Fil: Camiletti, Jorge. Hospital Italiano de La Plata; ArgentinaFil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Hospital Español de Mendoza; ArgentinaFil: López Santi, Ricardo. Hospital Italiano de La Plata; ArgentinaFil: Erriest, Juan. Hospital Italiano de La Plata; ArgentinaFil: García-Bello, Eliomar. Centro de Diagnóstico Medicina Avanzada y Telemedicina; República DominicanaFil: Araujo, John. Centro Cardiovascular Somer Incare; ColombiaFil: Varleta-Olivares, Paola. Hospital Dipreca; ChileFil: Gómez-Díaz, Eduardo. Hospital Metropolitano del Norte; VenezuelaFil: Ramírez, Gisselle. Medicina Cardiovascular Asociada; República DominicanaFil: Berni Betancourt, Ana. Sociedad interamericana de Cardiología; México. Consejo Interamericano de Electrocardiográfica y Arritmias; México. Hospital Ángeles Pedregal; MéxicoFil: Escalada Lesme, Gustavo. Centro Médico Nacional-Hospital Nacional Itaguá; ParaguayFil: Campos Alcántara, Lourdes V.. Consultorio de Lourdes Victoria Campos Alcántara; PerúFil: Moya Loor, Leonardo. Hospital Santa Margarita; EcuadorFil: Rey Benavente, Claudio. Hospital Arroyabe Pichanal; ArgentinaFil: Almonte, Claudia. Medicina Cardiovascular Asociada; República DominicanaFil: Cortez Sandoval, Maicol. Hospital Nacional Edgardo Rebagliti Martins; PerúFil: Alvarado Cuadros, María. Department of Cardiology, Institution; EcuadorFil: Rosario, Monica I.. Centro de Diagnóstico Medicina Avanzada y Telemedicina; República DominicanaFil: Gupta, Shyla. Queen’s University; CanadáFil: Ibarrola, Martin. Cardiovascular Center BV; ArgentinaFil: Baranchuk, Adrián. Kingston Health Sciences Centre; Canad

    HPV types and cofactors causing cervical cancer in Peru

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    We conducted a hospital-based case-control study in Peru of 198 women with histologically confirmed cervical cancer (173 squamous cell carcinomas and 25 cases of adenocarcinoma/adenosquamous carcinoma) and 196 control women. Information on risk factors was obtained by personal interview. Using PCR-based assays on exfoliated cervical cells and biopsy specimens, HPV DNA was detected in 95.3% of women with squamous cell carcinoma and in 92.0% of women with adenocarcinoma/adenosquamous carcinoma compared with 17.7% in control women. The age-adjusted odds ratio was 116.0 (95% Cl = 48.6–276.0) for squamous cell carcinoma and 51.4 (95% Cl = 11.4–232.0) for adenocarcinoma/adenosquamous carcinoma. The commonest types in women with cervical cancer were HPV 16, 18, 31, 52 and 35. The association with the various HPV types was equally strong for the two most common types (HPV 16 and 18) as for the other less common types. In addition to HPV, long-term use of oral contraceptives and smoking were associated with an increased risk. HPV is the main cause of both squamous cell carcinoma and adenocarcinoma in Peruvian women. © 2001 Cancer Research Campaignhttp://www.bjcancer.co

    SOIL FERTILITY EVALUATION OF COFFEE (Coffea spp.) PRODUCTION SYSTEMS AND MANAGEMENT RECOMMENDATIONS FOR THE BARAHONA PROVINCE, DOMINICAN REPUBLIC

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    ABSTRACT Reported yields in most coffee farms of the Barahona province in the Dominican Republic are relatively low (&lt; 290 kg ha -1 parchment coffee). In general, coffee producers do not use diagnostic techniques such as soil testing. This fact prevents them from identifying the limiting factors (especially nutrients), complicates the work of coffee cultural management practices, and potentially reduces productivity and coffee quality. This study was designed to diagnose the fertility level of soils in coffee farms in the area of Barahona in 96 farms within an area of 637 hectares and design a nutrient management strategy. Soils from each farm were sampled and analyzed for soil fertility parameters. A survey was provided to farmers that permitted the collection of information regarding yields, management practices, and landscape features. Soils in the area were predominantly clayey. Soil pH varied between 4.61 and 7.69 and soil organic matter ranged between 3.29 and 10.9%. Exchangeable potassium levels were classified as deficient in all areas. The clustering of results identified two main components, which accounted for 76% of the variability of the data and the grouping into five communities by similarity of features. The results show that soil testing of this coffee coffee-growing region can be used as a tool to diagnose the soil fertility status and guide them in implementing management and fertilization recommendations

    The Lantern, 2021-2022

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    No More Buses through El Paso • A Woman\u27s World • The Angel of Tragedy • A Victim of Circumstance • Ace of Hearts • Ghost Light • Missing Diamonds • The Upside-Down House: A Dialogue with the Self • What is Chronic Pain? • A Sunny Day in Sinkhole • Extra Marshmallows • Fourth Wall Broken • Hemlock • In the Comfort of Others • Lasting Impressions • Let\u27s Do the Time Warp Again • One Last Afternoon • Space Invaders • The Dogwood Tree • An Ode to Poppies • Charlotte\u27s Web • Crab • Crossing • Dandelions • Dandelion Sandwich • Grizzly Hood • Help Wanted • I Gave Way • I\u27m not who you wanted but maybe one day I can be • Kneeling • Lemon Cookies • Lies • Method Acting • Moment of Tranquility • Our Home • Overthinking • Sea Glass • Seasonal • Thirty-Two (No Spares) • The Autumn Beast • The Miller\u27s Daughter • Theodore • To the Earring I Left Behind in Your Carpet • Virginia • Waltzing • Yellow House • 1/25 British Monarch • Cracked • In the Shadows • Jewelwing • Life on the Wing • O\u27 Captain my Captain • Stars Above the Bay • The Common Fall • Tom • Cats + Crowshttps://digitalcommons.ursinus.edu/lantern/1190/thumbnail.jp

    Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

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    In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field
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