344 research outputs found
Human tribbles-1 controls proliferation and chemotaxis of smooth muscle cells via MAPK signaling pathways
Migration and proliferation of smooth muscle cells are key to a number of physiological and pathological processes, including wound healing and the narrowing of the vessel wall.Previous work has shown links between inflammatory stimuli and vascular smooth muscle cell proliferation and migration through mitogen activated protein kinase (MAPK) activation, though the molecular mechanisms of this process are poorly understood.
Here we report that tribbles-1, a recently described modulator of MAPK activation controls vascular smooth muscle cell proliferation and chemotaxis via the Jun Kinase pathway. Our findings demonstrate that this regulation takes place via direct interactions between tribbles-1 and MKK4/SEK1, a Jun activator kinase. The
activity of this kinase is dependent on tribbles-1 levels, whilst the activation and the expression of MKK4/SEK1 is not. In addition, tribbles-1 expression is elevated in
human atherosclerotic arteries compared to non-atherosclerotic controls, suggesting that this protein may pay a role in disease in vivo. In summary, the data presented here suggest an important regulatory role for trb-1 in vascular smooth muscle cell biology
The radius and effective temperature of the binary Ap star beta CrB from CHARA/FLUOR and VLT/NACO observations
The prospects for using asteroseismology of rapidly oscillating Ap (roAp)
stars are hampered by the large uncertainty in fundamental stellar parameters.
Results in the literature for the effective temperature (Teff) often span a
range of 1000 K. Our goal is to reduce systematic errors and improve the Teff
calibration of Ap stars based on new interferometric measurements. We obtained
long-baseline interferometric observations of beta CrB using the CHARA/FLUOR
instrument. To disentangle the flux contributions of the two components of this
binary star, we obtained VLT/NACO adaptive optics images. We determined limb
darkened angular diameters of 0.699+-0.017 mas for beta CrB A (from
interferometry) and 0.415+-0.017 mas for beta CrB B (from surface brightness-
color relations), corresponding to radii of 2.63+-0.09 Rsun (3.4 percent
uncertainty) and 1.56+-0.07 Rsun (4.5 percent). The combined bolometric flux of
the A and B components was determined from satellite UV data, spectrophotometry
in the visible and broadband data in the infrared. The flux from the B
component constitutes 16+-4 percent of the total flux and was determined by
fitting an ATLAS9 model atmosphere to the broad-band NACO J and K magnitudes.
Combining the flux of the A component with its measured angular diameter, we
determine the effective temperature Teff(A) = 7980+-180 K (2.3 percent). Our
new interferometric and imaging data enable a nearly model-independent
determination of the effective temperature of beta CrB A. Including our recent
study of alpha Cir, we now have direct Teff measurements of two of the
brightest roAp stars, providing a strong benchmark for an improved calibration
of the Teff scale for Ap stars. This will support the use of potentially strong
constraints imposed by asteroseismic studies of roAp stars.Comment: 7 pages, accepted by A&
Nernst effect of the new iron-based superconductor LaOFFeAs
We report the first Nernst effect measurement on the new iron-based
superconductor LaOFFeAs . In the normal state, the
Nernst signal is negative and very small. Below a large positive peak
caused by vortex motion is observed. The flux flowing regime is quite large
compared to conventional type-II superconductors. However, a clear deviation of
the Nernst signal from normal state background and an anomalous depression of
off-diagonal thermoelectric current in the normal state between and 50
K are observed. We propose that this anomaly in the normal state Nernst effect
could correlate with the SDW fluctuations.Comment: 8 pages, 4 figures; Latex file changed, references adde
The effects of electrical hippocampal kindling of seizures on amino acids and kynurenic acid concentrations in brain structures
Our study demonstrated that the development of seizures during the electrically induced kindling of seizures is associated with significant changes in the concentration of kynurenic acid (KYNA) and its precursor, tryptophan (TRP). The primary finding of our study was an increase in KYNA levels and the KYNA/TRP ratio (a theoretical index of activity of the kynurenine pathway) in the amygdala and hippocampus of kindled animals. We also found decreases in the concentration of tryptophan in the hippocampus and prefrontal cortex. Changes in the concentration of KYNA and TRP in the amygdala were accompanied by a significant decrease in γ-Aminobutryic Acid (GABA) levels and an increase in the glutamate/GABA ratio. Moreover, we found a significant negative correlation between the local concentrations of KYNA and glutamate in the amygdala of kindled rats. However, there were no changes in the local concentrations of the following amino acids: glutamate, aspartate, glutamine, glycine, taurine and alanine. In conclusion, these new results suggest a modulatory influence of KYNA on the process of epileptogenesis, characterized by a negative relationship between the KYNA and glutamate systems in the amygdala
Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis
Megakaryocytopoiesis is a complex differentiation process driven by the hormone
thrombopoietin by which haematopoietic progenitor cells give rise to
megakaryocytes, the giant bone marrow cells that in turn break down to form blood
platelets. The Tribbles Pseudokinase 3 gene (TRIB3) encodes a pleiotropic protein
increasingly implicated in the regulation of cellular differentiation programmes.
Previous studies have hinted that TRIB3 could be also involved in
megakaryocytopoiesis but its role in this process has so far not been investigated.
Using cellular model systems of haematopoietic lineage differentiation here we
demonstrate that TRIB3 is a negative modulator of megakaryocytopoiesis. We found
that in primary cultures derived from human haematopoietic progenitor cells,
thrombopoietin-induced megakaryocytic differentiation led to a time and dosedependent
decrease in TRIB3 mRNA levels. In the haematopoietic cell line UT7/mpl,
silencing of TRIB3 increased basal and thrombopoietin-stimulated megakaryocyte
antigen expression, as well as basal levels of ERK1/2 phosphorylation. In primary
haematopoietic cell cultures, silencing of TRIB3 facilitated megakaryocyte
differentiation. In contrast, over-expression of TRIB3 in these cells inhibited the
differentiation process. The in-vitro identification of TRIB3 as a negative regulator of
megakaryocytopoiesis suggests that in-vivo this gene could be important for the
regulation of platelet production
Formation of dense partonic matter in relativistic nucleus-nucleus collisions at RHIC: Experimental evaluation by the PHENIX collaboration
Extensive experimental data from high-energy nucleus-nucleus collisions were
recorded using the PHENIX detector at the Relativistic Heavy Ion Collider
(RHIC). The comprehensive set of measurements from the first three years of
RHIC operation includes charged particle multiplicities, transverse energy,
yield ratios and spectra of identified hadrons in a wide range of transverse
momenta (p_T), elliptic flow, two-particle correlations, non-statistical
fluctuations, and suppression of particle production at high p_T. The results
are examined with an emphasis on implications for the formation of a new state
of dense matter. We find that the state of matter created at RHIC cannot be
described in terms of ordinary color neutral hadrons.Comment: 510 authors, 127 pages text, 56 figures, 1 tables, LaTeX. Submitted
to Nuclear Physics A as a regular article; v3 has minor changes in response
to referee comments. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
Role of Cajal Bodies and Nucleolus in the Maturation of the U1 snRNP in Arabidopsis
Background: The biogenesis of spliceosomal snRNPs takes place in both the cytoplasm where Sm core proteins are added and snRNAs are modified at the 59 and 39 termini and in the nucleus where snRNP-specific proteins associate. U1 snRNP consists of U1 snRNA, seven Sm proteins and three snRNP-specific proteins, U1-70K, U1A, and U1C. It has been shown previously that after import to the nucleus U2 and U4/U6 snRNP-specific proteins first appear in Cajal bodies (CB) and then in splicing speckles. In addition, in cells grown under normal conditions U2, U4, U5, and U6 snRNAs/snRNPs are abundant in CBs. Therefore, it has been proposed that the final assembly of these spliceosomal snRNPs takes place in this nuclear compartment. In contrast, U1 snRNA in both animal and plant cells has rarely been found in this nuclear compartment. Methodology/Principal Findings: Here, we analysed the subnuclear distribution of Arabidopsis U1 snRNP-specific proteins fused to GFP or mRFP in transiently transformed Arabidopsis protoplasts. Irrespective of the tag used, U1-70K was exclusively found in the nucleus, whereas U1A and U1C were equally distributed between the nucleus and the cytoplasm. In the nucleus all three proteins localised to CBs and nucleoli although to different extent. Interestingly, we also found that the appearance of the three proteins in nuclear speckles differ significantly. U1-70K was mostly found in speckles whereas U1A and U1C in,90 % of cells showed diffuse nucleoplasmic in combination with CBs and nucleolar localisation. Conclusions/Significance: Our data indicate that CBs and nucleolus are involved in the maturation of U1 snRNP. Difference
Role of Cajal Bodies and Nucleolus in the Maturation of the U1 snRNP in Arabidopsis
Background: The biogenesis of spliceosomal snRNPs takes place in both the cytoplasm where Sm core proteins are added and snRNAs are modified at the 59 and 39 termini and in the nucleus where snRNP-specific proteins associate. U1 snRNP consists of U1 snRNA, seven Sm proteins and three snRNP-specific proteins, U1-70K, U1A, and U1C. It has been shown previously that after import to the nucleus U2 and U4/U6 snRNP-specific proteins first appear in Cajal bodies (CB) and then in splicing speckles. In addition, in cells grown under normal conditions U2, U4, U5, and U6 snRNAs/snRNPs are abundant in CBs. Therefore, it has been proposed that the final assembly of these spliceosomal snRNPs takes place in this nuclear compartment. In contrast, U1 snRNA in both animal and plant cells has rarely been found in this nuclear compartment. Methodology/Principal Findings: Here, we analysed the subnuclear distribution of Arabidopsis U1 snRNP-specific proteins fused to GFP or mRFP in transiently transformed Arabidopsis protoplasts. Irrespective of the tag used, U1-70K was exclusively found in the nucleus, whereas U1A and U1C were equally distributed between the nucleus and the cytoplasm. In the nucleus all three proteins localised to CBs and nucleoli although to different extent. Interestingly, we also found that the appearance of the three proteins in nuclear speckles differ significantly. U1-70K was mostly found in speckles whereas U1A and U1C in,90 % of cells showed diffuse nucleoplasmic in combination with CBs and nucleolar localisation. Conclusions/Significance: Our data indicate that CBs and nucleolus are involved in the maturation of U1 snRNP. Difference
Measurements of long-range azimuthal anisotropies and associated Fourier coefficients for pp collisions at √s=5.02 and 13 TeV and p+Pb collisions at √sNN=5.02 TeV with the ATLAS detector
ATLAS measurements of two-particle correlations are presented for √s=5.02 and 13 TeV ppcollisions and for √sNN=5.02 TeV p+Pb collisions at the LHC. The correlation functions are measured as a function of relative azimuthal angle Δϕ, and pseudorapidity separation Δη, using charged particles detected within the pseudorapidity interval |η|2, is studied using a template fitting procedure to remove a “back-to-back” contribution to the correlation function that primarily arises from hard-scattering processes. In addition to the elliptic, cos (2Δϕ), modulation observed in a previous measurement, the pp correlation functions exhibit significant cos (3Δϕ) and cos (4Δϕ) modulation. The Fourier coefficients vn, n associated with the cos (nΔϕ) modulation of the correlation functions for n=2–4 are measured as a function of charged-particle multiplicity and charged-particle transverse momentum. The Fourier coefficients are observed to be compatible with cos (nϕ) modulation of per-event single-particle azimuthal angle distributions. The single-particle Fourier coefficients vn are measured as a function of charged-particle multiplicity, and charged-particle transverse momentum for n=2–4. The integrated luminosities used in this analysis are, 64nb−1 for the √s=13 TeV pp data, 170 nb−1 for the √ s = 5.02 TeV pp data, and 28 nb−1 for the √sNN = 5.02 TeV p+Pb data
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