6 research outputs found
The Effect of Incomplete Bile Duct Obstruction on Diisopropanolnitrosamine—Induced Cholangiocarcinoma
This study was carried out to clarify the influence of incomplete bile duct obstruction (IBDO) on the
occurrence and proliferation of cholangiocarcinoma and to evaluate the effect of release of IBDO at an
early stage, using 175 Syrian golden hamsters. These hamsters received 500mg/kg body weight of
diisopropanolnitrosamine (DIPN) once weekly for 10 weeks, and then were divided into 3 groups,
consisting of the simple laparotomy group (SL group), the IBDO group and 2 week IBDO group, in
which IBDO was released after 2 weeks. The occurrence rates of cholangiocarcinoma at 20 weeks were
42% in the SL group, 76% in the IBDO group and 30% in the 2 week IBDO group. The mean numbers
of tumors per hamster in the IBDO group were significantly greater than those in other groups (p <
0.05). Both occurrence rates and numbers of tumors in the 2 week IBDO group were similar to those in
the SL group. The proliferation of bile ductules and isolation of bacteria from bile in the IBDO group
had higher rates at 15, 20 weeks than those found in the other groups. These results suggest that IBDO
has an influence, as promoter, on the occurrence of cholangiocarcinoma induced by DIPN, and the
disappearance of its promoting effect is caused by release of the obstruction