122 research outputs found
Simplified Kinetics and Colour Formation in Sucrose Solutions Based on A-Dicarbonyl Compounds
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugÀnglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Colour formation in technical and model sucrose solutions was investigated resulting in a novel kinetic approach of MAILLARD reaction during thermal processing of sugar solutions. Presented results describe new aspects of the non-enzymatic browning reaction (MAILLARD reaction). Two temperature depending pathways of colour formation were found. Both reaction mechanisms are based on the formation of a-dicarbonyl compounds, the key intermediates of colour formation.Discussing temperature dependence of colour formation, a change on MAILLARD reaction mechanism takes place at 100.4 °C. Above this temperature the colour formation is strongly accelerated. Activation energy of the non-enzymatic browning energy for temperatures from 65 ° to 100.4 °C amounts 77 kJ/mol. In this temperature range, D-glucosone is the most important a-dicarbonyl compound for studied reaction systems. Above 100.4 °C, activation energy is equal to 112 kJ/mol and 3-deoxyosone is the dominant colour formation intermediate. Achieved results bridge the gap between the termination step of a MAILLARD reaction i.e. of colour formation (represented by its activation energy) and intermediates formation (reaction kinetics). In particular, a change of colour formation mechanism with reaction temperature was confirmed by specific formation of two a-dicarbonyl compounds, responsible for MAILLARD reaction in technical sugar solutions
Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
<p>Abstract</p> <p>Background</p> <p>The <it>Streptococcus pyogenes </it>(group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis of this exclusively human bacterial species. If this system acts uniquely in all serotypes is currently unclear. Presence of serotype- or strain-dependent regulatory circuits and polarity is an emerging scheme in <it>Streptococcus pyogenes </it>pathogenesis. Thus, the contribution of the sensor kinase (CovS) of the global regulatory two-component signal transduction system CovRS on pathogenesis of several M serotypes was investigated.</p> <p>Results</p> <p>CovS mutation uniformly repressed capsule expression and hampered keratinocyte adherence in all tested serotypes. However, a serotype- and even strain-dependent contribution on survival in whole human blood and biofilm formation was noted, respectively.</p> <p>Conclusions</p> <p>These data provide new information on the action of the CovS sensor kinase and revealed that its activity on capsule expression and keratinocyte adherence is uniform across serotypes, whereas the influence on biofilm formation and blood survival is serotype or even strain dependent. This adds the CovRS system to a growing list of serotype-specific acting regulatory loci in <it>S. pyogenes</it>.</p
Bright Room-Temperature Single Photon Emission from Defects in Gallium Nitride
Single photon emitters play a central role in many photonic quantum
technologies. A promising class of single photon emitters consists of atomic
color centers in wide-bandgap crystals, such as diamond silicon carbide and
hexagonal boron nitride. However, it is currently not possible to grow these
materials as sub-micron thick films on low-refractive index substrates, which
is necessary for mature photonic integrated circuit technologies. Hence, there
is great interest in identifying quantum emitters in technologically mature
semiconductors that are compatible with suitable heteroepitaxies. Here, we
demonstrate robust single photon emitters based on defects in gallium nitride
(GaN), the most established and well understood semiconductor that can emit
light over the entire visible spectrum. We show that the emitters have
excellent photophysical properties including a brightness in excess of 500x10^3
counts/s. We further show that the emitters can be found in a variety of GaN
wafers, thus offering reliable and scalable platform for further technological
development. We propose a theoretical model to explain the origin of these
emitters based on cubic inclusions in hexagonal gallium nitride. Our results
constitute a feasible path to scalable, integrated on-chip quantum technologies
based on GaN
Analysis of the influence of drainage channels in environmental degradation in the flooded plain of ParanĂĄ river, Brazil
Artigo extraĂdo de dissertação de Mestrado em CiĂȘncias Florestais do Departamento de Engenharia Florestal â UnB.Agradecimentos ao CNPq, CAPES, EMBRAPA Pantanal, CESP, Fundação Giacometti e EMBRAPA-CENARGEN.A pesquisa teve como objetivo analisar a influĂȘncia dos canais de drenagem artificiais construĂdos para ampliar a ĂĄrea agrĂcola e de pastagem nas ĂĄreas Ășmidas na planĂcie de inundação do rioParanĂĄ, utilizando um Sistema de Informação GeogrĂĄfica (SIG). Foram selecionadas imagens de satĂ©lite nas Ă©pocas da seca e da chuva. A partir de transectos obtidos nos sobrevĂŽos na ĂĄrea, foram coletados 50 pontos com canal e sem canal, com o auxĂlio de um GPS. Esses pontos foram plotados nas imagens classificadas. Em seguida foram gerados âbuffersâ de 1 km de raio.A partir destes âbuffersâ foi analisada a disponibilidade das ĂĄreas de vĂĄrzea (campo alagado e Ășmido) nas Ă©pocas seca e chuvosa. O campo alagado na Ă©poca da cheia nĂŁo foi influenciadopela presença de canais de drenagem, enquanto que na seca os canais de drenagem influenciaram a disponibilidade destas ĂĄreas. O mesmo nĂŁo aconteceu com o campo Ășmido que sofreu influĂȘncia dos canais de drenagem tanto na Ă©poca seca quanto na Ă©poca da chuva. A redução de campo alagado na seca, em ĂĄreas com canal, foi de 56,2% e em ĂĄreas sem canal foi de 9%. ______________________________________________________________________________ ABSTRACTThe main aim of this study was to analyse the influence of the artificial drainage channelsconstructed to enlarge pasture at the plain humid areas of the ParanĂĄ river, using the GIS system.To achieve it were collected satellite images during the dry and wet seasons. Through over flytransects of the area were collected 50 spots with and without channels, with the aid of a GPS system. These spots were plotted at the satellite images that were processed generating buffers of 1 km range. From these buffers were analysed the availability of varzea area (flooded andhumid fields) during both periods. The flooded fields during the high waters season were notaffected by the presence of drainage channels, while during the dry season the drainage channelsaffected these areas. The same did not happen with the humid fields, that during both seasons suffered the influence of the drainage channels. The flooded area during the dry season reduced56.2% in the areas with drainage channels, and 9% in the areas without channel
The colonic mucosa-associated microbiome in SIV infection: shift towards Bacteroidetes coincides with mucosal CD4(+) T cell depletion and enterocyte damage
Allers K, Stahl-Hennig C, Fiedler T, et al. The colonic mucosa-associated microbiome in SIV infection: shift towards Bacteroidetes coincides with mucosal CD4(+) T cell depletion and enterocyte damage. SCIENTIFIC REPORTS. 2020;10(1).The intesinal microbiome is considered important in human immunodeficiency virus (HIV) pathogenesis and therefore represents a potential therapeutic target to improve the patients' health status. Longitudinal alterations in the colonic mucosa-associated microbiome during simian immunodeficiency virus (SIV) infection were investigated using a 16S rRNA amplicon approach on the Illumina sequencing platform and bioinformatics analyses. Following SIV infection of six animals, no alterations in microbial composition were observed before the viral load peaked in the colon. At the time of acute mucosal SIV replication, the phylum Bacteroidetes including the Bacteroidia class as well as the phylum Firmicutes and its families Ruminococcaceae and Eubacteriaceae became more abundant. Enrichment of Bacteroidetes was maintained until the chronic phase of SIV infection. The shift towards Bacteroidetes in the mucosa-associated microbiome was associated with the extent of SIV infection-induced mucosal CD4(+) T cell depletion and correlated with increasing rates of enterocyte damage. These observations suggest that Bacteroidetes strains increase during virus-induced mucosal immune destruction. As Bacteroidetes belong to the lipopolysaccharide- and short chain fatty acids-producing bacteria, their rapid enrichment may contribute to inflammatory tissue damage and metabolic alterations in SIV/HIV infection. These aspects should be considered in future studies on therapeutic interventions
Molecular Interactions of Prodiginines with the BH3 Domain of Anti-Apoptotic Bcl-2 Family Members
Prodigiosin and obatoclax, members of the prodiginines family, are small molecules with anti-cancer properties that are
currently under preclinical and clinical trials. The molecular target(s) of these agents, however, is an open question.
Combining experimental and computational techniques we find that prodigiosin binds to the BH3 domain in some BCL-2
protein families, which play an important role in the apoptotic programmed cell death. In particular, our results indicate
a large affinity of prodigiosin for MCL-1, an anti-apoptotic member of the BCL-2 family. In melanoma cells, we demonstrate
that prodigiosin activates the mitochondrial apoptotic pathway by disrupting MCL-1/BAK complexes. Computer simulations
with the PELE software allow the description of the induced fit process, obtaining a detailed atomic view of the molecular
interactions. These results provide new data to understand the mechanism of action of these molecules, and assist in the
development of more specific inhibitors of anti-apoptotic BCL-2 proteins.Spanish government and the European Union (FIS-PI10/00338) and from the ERC-2009-Adg
25027-PELE European project
STXBP1 promotes Weibel-Palade body exocytosis through its interaction with the Rab27A effector Slp4-a.
Vascular endothelial cells contain unique rod-shaped secretory organelles, called Weibel-Palade bodies (WPBs), which contain the hemostatic protein von Willebrand factor (VWF) and a cocktail of angiogenic and inflammatory mediators. We have shown that the Rab27A effector synaptotagmin-like protein 4-a (Slp4-a) plays a critical role in regulating hormone-evoked WPB exocytosis. Using a nonbiased proteomic screen for targets for Slp4-a, we now identify syntaxin-binding protein 1 (STXBP1) and syntaxin-2 and -3 as endogenous Slp4-a binding partners in endothelial cells. Coimmunoprecipitations showed that STXBP1 interacts with syntaxin-2 and -3, but not with syntaxin-4. Small interfering RNA-mediated silencing of STXBP1 expression impaired histamine- and forskolin-induced VWF secretion. To further substantiate the role of STXBP1, we isolated blood outgrowth endothelial cells (BOECs) from an early infantile epileptic encephalopathy type 4 (EIEE4) patient carrying a de novo mutation in STXBP1. STXBP1-haploinsufficient EIEE4 BOECs contained similar numbers of morphologically normal WPBs compared with control BOECs of healthy donors; however, EIEE4 BOECs displayed significantly impaired histamine- and forskolin-stimulated VWF secretion. Based on these findings, we propose that the Rab27A-Slp4-a complex on WPB promotes exocytosis through an interaction with STXBP1, thereby controlling the release of vaso-active substances in the vasculature
Phytoplankton across Tropical and Subtropical Regions of the Atlantic, Indian and Pacific Oceans
Postprint4,411
A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
The complex changes in the life cycle of Clostridium acetobutylicum, a promising biofuel producer, are not well understood. During exponential growth, sugars are fermented to acetate and butyrate, and in the transition phase, the metabolism switches to the production of the solvents acetone and butanol accompanied by the initiation of endospore formation. Using phosphate-limited chemostat cultures at pHÂ 5.7, C. acetobutylicum was kept at a steady state of acidogenic metabolism, whereas at pHÂ 4.5, the cells showed stable solvent production without sporulation. Novel proteome reference maps of cytosolic proteins from both acidogenesis and solventogenesis with a high degree of reproducibility were generated. Yielding a 21% coverage, 15 protein spots were specifically assigned to the acidogenic phase, and 29 protein spots exhibited a significantly higher abundance in the solventogenic phase. Besides well-known metabolic proteins, unexpected proteins were also identified. Among these, the two proteins CAP0036 and CAP0037 of unknown function were found as major striking indicator proteins in acidogenic cells. Proteome data were confirmed by genome-wide DNA microarray analyses of the identical cultures. Thus, a first systematic study of acidogenic and solventogenic chemostat cultures is presented, and similarities as well as differences to previous studies of batch cultures are discussed
Search for dark matter produced in association with bottom or top quarks in âs = 13 TeV pp collisions with the ATLAS detector
A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fbâ1 of protonâproton collision data recorded by the ATLAS experiment at âs = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements
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