261 research outputs found
Loss of Endothelial Glycocalyx During Normothermic Machine Perfusion of Porcine Kidneys Irrespective of Pressure and Hematocrit
BACKGROUND: Normothermic machine perfusion (NMP) is a promising modality for marginal donor kidneys. However, little is known about the effects of NMP on causing endothelial glycocalyx (eGC) injury. This study aims to evaluate the effects of NMP on eGC injury in marginal donor kidneys and whether this is affected by perfusion pressures and hematocrits.METHODS: Porcine slaughterhouse kidneys (n = 6/group) underwent 35âmin of warm ischemia. Thereafter, the kidneys were preserved with oxygenated hypothermic machine perfusion for 3âh. Subsequently, 4âh of NMP was applied using pressure-controlled perfusion with an autologous blood-based solution containing either 12%, 24%, or 36% hematocrit. Pressures of 55, 75, and 95 mmâ
Hg were applied in the 24% group. Perfusate, urine, and biopsy samples were collected to determine both injury and functional parameters.RESULTS: During NMP, hyaluronan levels in the perfusate increased significantly ( P < 0.0001). In addition, the positivity of glyco-stained glycocalyx decreased significantly over time, both in the glomeruli ( P = 0.024) and peritubular capillaries ( P = 0.003). The number of endothelial cells did not change during NMP ( P = 0.157), whereas glomerular endothelial expression of vascular endothelial growth factor receptor-2 decreased significantly ( P < 0.001). Microthrombi formation was significantly increased after NMP. The use of different pressures and hematocrits did not affect functional parameters during perfusion. CONCLUSIONS: NMP is accompanied with eGC and vascular endothelial growth factor receptor-2 loss, without significant loss of endothelial cells. eGC loss was not affected by the different pressures and hematocrits used. It remains unclear whether endothelial injury during NMP has harmful consequences for the transplanted kidney.</p
Associated Charm Production in Neutrino-Nucleus Interactions
In this paper a search for associated charm production both in neutral and
charged current -nucleus interactions is presented. The improvement of
automatic scanning systems in the {CHORUS} experiment allows an efficient
search to be performed in emulsion for short-lived particles. Hence a search
for rare processes, like the associated charm production, becomes possible
through the observation of the double charm-decay topology with a very low
background. About 130,000 interactions located in the emulsion target
have been analysed. Three events with two charm decays have been observed in
the neutral-current sample with an estimated background of 0.180.05. The
relative rate of the associated charm cross-section in deep inelastic
interactions, has been
measured. One event with two charm decays has been observed in charged-current
interactions with an estimated background of 0.180.06 and the
upper limit on associated charm production in charged-current interactions at
90% C.L. has been found to be .Comment: 10 pages, 4 figure
Circulating Mycobacterium tuberculosis DosR latency antigen-specific, polyfunctional, regulatory IL10(+) Th17 CD4 T-cells differentiate latent from active tuberculosis
Immunogenetics and cellular immunology of bacterial infectious disease
Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in âs = 7 TeV pp collisions with the ATLAS detector
A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fbâ1 of protonâproton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results
Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC
Measurements of inclusive jet suppression in heavy ion collisions at the LHC
provide direct sensitivity to the physics of jet quenching. In a sample of
lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated
luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with
a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the
transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the
anti-kt algorithm with values for the distance parameter that determines the
nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of
the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp.
Jet production is found to be suppressed by approximately a factor of two in
the 10% most central collisions relative to peripheral collisions. Rcp varies
smoothly with centrality as characterized by the number of participating
nucleons. The observed suppression is only weakly dependent on jet radius and
transverse momentum. These results provide the first direct measurement of
inclusive jet suppression in heavy ion collisions and complement previous
measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables,
submitted to Physics Letters B. All figures including auxiliary figures are
available at
http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02
The free energy of NOAA active region AR 11029
The NOAA active region AR 11029 was a small but highly active sunspot region
which produced 73 GOES soft X-ray flares. The flares appear to show a departure
from the well known power-law frequency-size distribution. Specifically, too
few GOES C-class and no M-class flares were observed by comparison with a
power-law distribution (Wheatland in Astrophys. J. 710, 1324, 2010). This was
conjectured to be due to the region having insufficient magnetic energy to
power large events. We construct nonlinear force-free extrapolations of the
coronal magnetic field of active region AR 11029 using data taken on 24 October
by the SOLIS Vector-SpectroMagnetograph (SOLIS/VSM), and data taken on 27
October by the Hinode Solar Optical Telescope SpectroPolarimeter (Hinode/SP).
Force-free modeling with photospheric magnetogram data encounters problems
because the magnetogram data are inconsistent with a force-free model, and we
employ a recently developed `self-consistency' procedure which addresses this
and accommodates uncertainties in the boundary data (Wheatland and Regnier in
Astrophys. J. 700, L88, 2009). We calculate the total energy and free energy of
the self-consistent solution and find that the free energy was 4x10^29 erg on
24 October, and 7x10^31 erg on 27 October. An order of magnitude scaling
between RHESSI non-thermal energy and GOES peak X-ray flux is established from
a sample of flares from the literature and is used to estimate flare energies
from observed GOES peak X-ray flux. Based on the scaling, we conclude that the
estimated free energy of AR 11029 on 27 October when the flaring rate peaked is
sufficient to power M-class or X-class flares, and hence the modeling does not
appear to support the hypothesis that the absence of large flares is due to the
region having limited energy.Comment: Accepted for publication in Solar Physic
Measurement of charm production in neutrino charged-current interactions
The nuclear emulsion target of the CHORUS detector was exposed to the
wide-band neutrino beam of the CERN SPS of 27 GeV average neutrino energy from
1994 to 1997. In total about 100000 charged-current neutrino interactions with
at least one identified muon were located in the emulsion target and fully
reconstructed, using newly developed automated scanning systems. Charmed
particles were searched for by a program recognizing particle decays. The
observation of the decay in nuclear emulsion makes it possible to select a
sample with very low background and minimal kinematical bias. 2013
charged-current interactions with a charmed hadron candidate in the final state
were selected and confirmed through visual inspection. The charm production
rate induced by neutrinos relative to the charged-current cross-section is
measured to be sigma(nu_mu N -> mu- C X)/sigma(CC) = (5.75 +-0.32 stat +-0.30
syst)%. The charm production cross-section as a function of the neutrino energy
is also obtained. The results are in good agreement with previous measurements.
The charm-quark hadronization produces the following charmed hadrons with
relative fractions (in %): f_Dzero = 43.7+-4.5, f_Lambda_c^plus = 19.2+-4.2,
f_Dplus = 25.3+-4.2, and f_D_splus = 11.8+-4.7.Comment: 16 pages, 5 figure
Search for astrophysical electron antineutrinos in Super-Kamiokande with 0.01wt% gadolinium-loaded water
We report the first search result for the flux of astrophysical electron
antineutrinos for energies O(10) MeV in the gadolinium-loaded Super-Kamiokande
(SK) detector. In June 2020, gadolinium was introduced to the ultra-pure water
of the SK detector in order to detect neutrons more efficiently. In this new
experimental phase, SK-Gd, we can search for electron antineutrinos via inverse
beta decay with efficient background rejection and higher signal efficiency
thanks to the high efficiency of the neutron tagging technique. In this paper,
we report the result for the initial stage of SK-Gd with a exposure at 0.01% Gd mass concentration. No significant excess
over the expected background in the observed events is found for the neutrino
energies below 31.3 MeV. Thus, the flux upper limits are placed at the 90%
confidence level. The limits and sensitivities are already comparable with the
previous SK result with pure-water () owing
to the enhanced neutron tagging
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