Active APPL1 sequestration by Plasmodium favors liver-stage development

© 2022 The Authors. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/).Intracellular pathogens manipulate host cells to survive and thrive. Cellular sensing and signaling pathways are among the key host machineries deregulated to favor infection. In this study, we show that liver-stage Plasmodium parasites compete with the host to sequester a host endosomal-adaptor protein (APPL1) known to regulate signaling in response to endocytosis. The enrichment of APPL1 at the parasitophorous vacuole membrane (PVM) involves an atypical Plasmodium Rab5 isoform (Rab5b). Depletion of host APPL1 alters neither the infection nor parasite development; however, upon overexpression of a GTPase-deficient host Rab5 mutant (hRab5_Q79L), the parasites are smaller and their PVM is stripped of APPL1. Infection with the GTPase-deficient Plasmodium berghei Rab5b mutant (PbRab5b_Q91L) in this case rescues the PVM APPL1 signal and parasite size. In summary, we observe a robust correlation between the level of APPL1 retention at the PVM and parasite size during exoerythrocytic development.This work was supported by grants from the la Caixa Banking Foundation and Fundação para a Ciência e a Tecnologia (HR17-00264 and PTDC/SAU-PAR/30751/2017 respectively, both to M.M.M.). A.L and S.S.B were sponsored by Fundação para a Ciência e a Tecnologia fellowships (PD/BD/114036/2015, SFRH/BD/114464/2016, respectively). V.S acknowledges funding from the Science and Engineering Research Board (SERB) (EMR/2016/006810), Department of Science and Technology (DST), Government of India. V.P. and C.S. were supported by GRK2581 (P6) SPHINGOINF of the Growing Spine Foundation (DFG).info:eu-repo/semantics/publishedVersio

O perfil do idoso assistido em serviço universitário ambulatorial

Objetivos: Elaborar un banco de datos de los ancianos asistidos en el Núcleo de Atenção ao Idoso – NAI/UFPE; identificar el perfil del anciano en función de variables sociales demográficas y de la salud; evaluar elresultado obtenido en un Micro Examen del Estado Mental (MEEM). Metodología: pesquisa de naturalezade exploración descriptiva que consistió en el análisis de 1194 relatos escritos de los ancianos participantesentre los años de 2000 y 2004. Resultados: Con relación a la distribución por género, 23,4% de los ancianosson del sexo masculino y 76,6% del femenino. En relación a la escolaridad 60% tenían menos de cuatroaños de estudio. El número de viudas (38%) fue tres veces mayor que el de los viudos (12%). Los registrosclínicos más prevalentes sobre la salud de los ancianos fueron: problema de visión, hipertensión arterial,antecedente quirúrgico, dislipidemía, vértigo, catarata, fractura del hueso, reacción alérgica y sedentarismo.El desarrollo en el Micro Examen del Estado Mental presentó una media 24 (DP = 3,7) y mediana 23,5.Destacase que 4,9% de los ancianos tienen resultados menores o iguales a 10 puntos. Conclusiones:Considerando la importancia integral en la atención en la salud, los datos investigados podrán subsidiar laestructuración de acciones dirigidas a clientela anciana atendida en el servicio del dispensario estudiado.Objectives: Develop a database of elder people assisted in the Núcleo de Atenção ao Idoso – NAI/UFPE;identify the profile of the elderly in terms of socio-demographic and health variables; evaluate the scoreobtained on the Mini Mental State Examination (MMSE). Methodology: Quantitative research of exploratorydescriptive nature consisting on the analysis of 1,194 medical records of elderly participants between2000 and 2004. Results: Concerning the distribution by gender, 23.4% of the elderly are male and 76.6%are female. In relation to education, 60% had less than four years of study. The number of female widows(38%) was three times higher than the number of male widows (12%). The most prevalent clinical recordsof the elderly people’s health were: visual problems, hypertension, surgical history, dyslipidemia, dizziness,cataract, bone fractures, allergic reaction and sedentism. The performance on the MMSE had an averageof 24 (SD = 3.7) and median of 23.5. It was found that 4.9% of seniors have scored less than 10 points.Conclusions: Considering the importance of completeness in health care, the data investigated can supportthe structure of actions directed to the elderly patients at the ambulatory place studied.Objetivos: Elaborar banco de dados dos idosos assistidos no Núcleo de Atenção ao Idoso – NAI/UFPE; identificar o perfil do idoso em função das variáveis sociodemográficas e de saúde; avaliar o escore obtido no Mini-Exame do Estado Mental (MEEM). Metodologia: Pesquisa quantitativa de natureza exploratório-descritiva que consistiu na análise de 1.194 prontuários médicos de idosos participantes entre 2000 a 2004. Resultados: Com respeito à distribuição por gênero, 23,4% dos idosos são do sexo masculino e 76,6% do feminino. Em relação à escolaridade 60% tinham menos de quatro anos de estudo. O número de viúvas (38%) foi três vezes maior que o de viúvos (12%). Os registros clínicos mais prevalentes sobre a saúde dos idosos foram: problema de visão, hipertensão arterial, antecedente cirúrgico, dislipidemia, tontura, catarata, fratura óssea, reação alérgica e sedentarismo. O desempenho no MEEM apresentou média 24 (DP = 3,7) e mediana 23,5. Destaca-se que 4,9% dos idosos têm escores menores ou iguais a 10 pontos. Conclusões: Considerando a importância da integralidade na atenção em saúde, os dados investigados poderão subsidiar a estruturação de ações dirigidas à clientela idosa atendida no serviço ambulatorial estudado

Auditory-perceptual judgment of velar stops associated to cleft palate by judges with and without experience

Objetivo: verificar: a) o grau de concordância de juízes no julgamento perceptivo-auditivo da produção de oclusivas velares, antes e depois da fonoterapia; b) a possível influência da composição fonética das amostras de fala nesta concordância e c) se os julgamentos obtidos por juízes com experiência diferem daqueles obtidos por juízes sem experiência, nas condições investigadas. Métodos: 60 amostras de fala de uma criança com fissura labiopalatina (30 previamente e 30 posteriormente a fonoterapia) e 30 amostras de fala de uma criança com fala típica (normal) foram julgadas por um grupo de 9 juízes. Três fonoaudiólogos estabeleceram os julgamentos consensuais "padrão ouro" para o estudo. Seis outros juízes julgaram as amostras: três considerados com experiência (fonoaudiólogos) e três sem experiência (alunos de graduação). As amostras de fala incluíram palavras constituídas pelas oclusivas velares /k/ e /g/ combinada com as vogais /a/, /i/ e /u/. Os juízes foram instruídos a julgar a presença, a ausência das oclusivas velares ou a presença de AC nestas amostras. Resultados: verificou-se diferenças no grau de concordância de juízes com experiência (Kappa moderada) e sem experiência (Kappa baixo) para os julgamentos realizados na condição pré-fonoterapia. O contexto fonético das amostras de fala influenciaram os julgamentos nas condições pré e pós-fonoterapia. Houve maior percentual de acerto para os juízes experientes condição pré-fonoterapia (p-valor <0,001). Conclusão: a experiência dos juízes e composição fonética das amostras de fala influenciam os julgamentos perceptivo-auditivos das AC.Purpose: to verify: a) the level of agreement among judges during auditory-perceptual assessment of velar plosive sounds before and after speech therapy, b) the possible influence of the phonetic composition from the speech samples in this correlation c) whether the assessments from judges with experience differ from those without experience in given conditions. Methods: 60 speech samples of children with cleft lip and palate, 30 before and 30 after speech therapy, and 30 samples from children without cleft lip and palate and with normal speech were rated by a group of 9 judges. Three speech-language pathologists established the "gold standard" consensual judgments for the study. Six other judges assessed the samples for this study: three considered experienced ones (speech-language pathologists) and three non-experienced (undergraduate students). The speech samples included velar consonants /k/ and /g/ and vowels /a/, /i/ and /u/. Judges were instructed to assess the presence or absence of velar consonants or presence of CA. Results: Kappa statistics revealed moderate agreement among experienced judges and low agreement among the judges without experience for samples recorded before speech therapy. Phonetic context had an effect on the assessments before and after speech therapy. Assessments were significantly better among experienced judges before speech therapy (p-valor <0,001). Conclusion: experience of judges and phonetic composition from the speech samples influence perceptual judgments of CA

Measurement of χ c1 and χ c2 production with s√ = 7 TeV pp collisions at ATLAS

The prompt and non-prompt production cross-sections for the χ c1 and χ c2 charmonium states are measured in pp collisions at s√ = 7 TeV with the ATLAS detector at the LHC using 4.5 fb−1 of integrated luminosity. The χ c states are reconstructed through the radiative decay χ c → J/ψγ (with J/ψ → μ + μ −) where photons are reconstructed from γ → e + e − conversions. The production rate of the χ c2 state relative to the χ c1 state is measured for prompt and non-prompt χ c as a function of J/ψ transverse momentum. The prompt χ c cross-sections are combined with existing measurements of prompt J/ψ production to derive the fraction of prompt J/ψ produced in feed-down from χ c decays. The fractions of χ c1 and χ c2 produced in b-hadron decays are also measured

Measurement of the production of a W boson in association with a charm quark in pp collisions at √s = 7 TeV with the ATLAS detector

The production of a W boson in association with a single charm quark is studied using 4.6 fb−1 of pp collision data at s√ = 7 TeV collected with the ATLAS detector at the Large Hadron Collider. In events in which a W boson decays to an electron or muon, the charm quark is tagged either by its semileptonic decay to a muon or by the presence of a charmed meson. The integrated and differential cross sections as a function of the pseudorapidity of the lepton from the W-boson decay are measured. Results are compared to the predictions of next-to-leading-order QCD calculations obtained from various parton distribution function parameterisations. The ratio of the strange-to-down sea-quark distributions is determined to be 0.96+0.26−0.30 at Q 2 = 1.9 GeV2, which supports the hypothesis of an SU(3)-symmetric composition of the light-quark sea. Additionally, the cross-section ratio σ(W + +c¯¯)/σ(W − + c) is compared to the predictions obtained using parton distribution function parameterisations with different assumptions about the s−s¯¯¯ quark asymmetry

Measurements of fiducial and differential cross sections for Higgs boson production in the diphoton decay channel at s√=8 TeV with ATLAS

Measurements of fiducial and differential cross sections are presented for Higgs boson production in proton-proton collisions at a centre-of-mass energy of s√=8 TeV. The analysis is performed in the H → γγ decay channel using 20.3 fb−1 of data recorded by the ATLAS experiment at the CERN Large Hadron Collider. The signal is extracted using a fit to the diphoton invariant mass spectrum assuming that the width of the resonance is much smaller than the experimental resolution. The signal yields are corrected for the effects of detector inefficiency and resolution. The pp → H → γγ fiducial cross section is measured to be 43.2 ±9.4(stat.) − 2.9 + 3.2 (syst.) ±1.2(lumi)fb for a Higgs boson of mass 125.4GeV decaying to two isolated photons that have transverse momentum greater than 35% and 25% of the diphoton invariant mass and each with absolute pseudorapidity less than 2.37. Four additional fiducial cross sections and two cross-section limits are presented in phase space regions that test the theoretical modelling of different Higgs boson production mechanisms, or are sensitive to physics beyond the Standard Model. Differential cross sections are also presented, as a function of variables related to the diphoton kinematics and the jet activity produced in the Higgs boson events. The observed spectra are statistically limited but broadly in line with the theoretical expectations

Search for direct pair production of the top squark in all-hadronic final states in proton-proton collisions at s√=8 TeV with the ATLAS detector

The results of a search for direct pair production of the scalar partner to the top quark using an integrated luminosity of 20.1fb−1 of proton–proton collision data at √s = 8 TeV recorded with the ATLAS detector at the LHC are reported. The top squark is assumed to decay via t˜→tχ˜01 or t˜→ bχ˜±1 →bW(∗)χ˜01 , where χ˜01 (χ˜±1 ) denotes the lightest neutralino (chargino) in supersymmetric models. The search targets a fully-hadronic final state in events with four or more jets and large missing transverse momentum. No significant excess over the Standard Model background prediction is observed, and exclusion limits are reported in terms of the top squark and neutralino masses and as a function of the branching fraction of t˜ → tχ˜01 . For a branching fraction of 100%, top squark masses in the range 270–645 GeV are excluded for χ˜01 masses below 30 GeV. For a branching fraction of 50% to either t˜ → tχ˜01 or t˜ → bχ˜±1 , and assuming the χ˜±1 mass to be twice the χ˜01 mass, top squark masses in the range 250–550 GeV are excluded for χ˜01 masses below 60 GeV

Search for squarks and gluinos with the ATLAS detector in final states with jets and missing transverse momentum using √s=8 TeV proton-proton collision data

A search for squarks and gluinos in final states containing high-p T jets, missing transverse momentum and no electrons or muons is presented. The data were recorded in 2012 by the ATLAS experiment in s√=8 TeV proton-proton collisions at the Large Hadron Collider, with a total integrated luminosity of 20.3 fb−1. Results are interpreted in a variety of simplified and specific supersymmetry-breaking models assuming that R-parity is conserved and that the lightest neutralino is the lightest supersymmetric particle. An exclusion limit at the 95% confidence level on the mass of the gluino is set at 1330 GeV for a simplified model incorporating only a gluino and the lightest neutralino. For a simplified model involving the strong production of first- and second-generation squarks, squark masses below 850 GeV (440 GeV) are excluded for a massless lightest neutralino, assuming mass degenerate (single light-flavour) squarks. In mSUGRA/CMSSM models with tan β = 30, A 0 = −2m 0 and μ > 0, squarks and gluinos of equal mass are excluded for masses below 1700 GeV. Additional limits are set for non-universal Higgs mass models with gaugino mediation and for simplified models involving the pair production of gluinos, each decaying to a top squark and a top quark, with the top squark decaying to a charm quark and a neutralino. These limits extend the region of supersymmetric parameter space excluded by previous searches with the ATLAS detector

Search for pair-produced long-lived neutral particles decaying to jets in the ATLAS hadronic calorimeter in ppcollisions at √s=8TeV

The ATLAS detector at the Large Hadron Collider at CERN is used to search for the decay of a scalar boson to a pair of long-lived particles, neutral under the Standard Model gauge group, in 20.3fb−1of data collected in proton–proton collisions at √s=8TeV. This search is sensitive to long-lived particles that decay to Standard Model particles producing jets at the outer edge of the ATLAS electromagnetic calorimeter or inside the hadronic calorimeter. No significant excess of events is observed. Limits are reported on the product of the scalar boson production cross section times branching ratio into long-lived neutral particles as a function of the proper lifetime of the particles. Limits are reported for boson masses from 100 GeVto 900 GeV, and a long-lived neutral particle mass from 10 GeVto 150 GeV