Characterization of an endogenous gene expressed in Aedes aegypti using an orally infectious recombinant Sindbis virus

Sindbis virus expression vectors have been used successfully to express and silence genes of interest in vivo in several mosquito species, including Aedes aegypti, Ae. albopictus, Ae. triseriatus,Culex pipiens, Armigeres subalbatus and Anopheles gambiae. Here we describe the expression of an endogenous gene, defensin, in Ae. aegypti using the orally infectious Sindbis virus, MRE/3′2J expression vector. We optimized conditions to infect mosquito larvae per os using C6/36 Ae. albopictus cells infected with the recombinant virus to maximize virus infection and expression of defensin. Infection with the parental Sindbis virus (MRE/3′2J) did not induce defensin expression. Mosquito larvae infected by ingestion of recombinant Sindbis virus-infected C6/36 cells expressed defensin when they emerged as adults. Defensin expression was observed by western analysis or indirect fluorescent assay in all developmental stages of mosquitoes infected with MRE/3′2J virus that contained the defensin insert. The multiplicity of infection of C6/36 cells and the quantity of infected cells consumed by larvae played an important role in defensin expression. Parental viruses, missing the defensin insert, and/or other defective interfering virus may have contributed to these observations

HiggsBounds: Confronting Arbitrary Higgs Sectors with Exclusion Bounds from LEP and the Tevatron

HiggsBounds is a computer code that tests theoretical predictions of models with arbitrary Higgs sectors against the exclusion bounds obtained from the Higgs searches at LEP and the Tevatron. The included experimental information comprises exclusion bounds at 95% C.L. on topological cross sections. In order to determine which search topology has the highest exclusion power, the program also includes, for each topology, information from the experiments on the expected exclusion bound, which would have been observed in case of a pure background distribution. Using the predictions of the desired model provided by the user as input, HiggsBounds determines the most sensitive channel and tests whether the considered parameter point is excluded at the 95% C.L. HiggsBounds is available as a Fortran 77 and Fortran 90 code. The code can be invoked as a command line version, a subroutine version and an online version. Examples of exclusion bounds obtained with HiggsBounds are discussed for the Standard Model, for a model with a fourth generation of quarks and leptons and for the Minimal Supersymmetric Standard Model with and without CP-violation. The experimental information on the exclusion bounds currently implemented in HiggsBounds will be updated as new results from the Higgs searches become available.Comment: 64 pages, 15 tables, 8 figures; three typos which made it to the published version corrected; the code (currently version 3.0.0beta including LHC Higgs search results) is available via: http://projects.hepforge.org/higgsbounds

Fiducial and differential cross sections of Higgs boson production measured in the four-lepton decay channel in pp collisions at √s = 8 TeV with the ATLAS detector

Measurements of fiducial and differential cross sections of Higgs boson production in the H→ZZ∗ → 4ℓ decay channel are presented. The cross sections are determined within a fiducial phase space and corrected for detection efficiency and resolution effects. They are based on 20.3 fb−¹ of pp collision data, produced at √s = 8 TeV centre-of-mass energy at the LHC and recorded by the ATLAS detector. The differential measurements are performed in bins of transverse momentum and rapidity of the four-lepton system, the invariant mass of the subleading lepton pair and the decay angle of the leading lepton pair with respect to the beam line in the four-lepton rest frame, as well as the number of jets and the transverse momentum of the leading jet. The measured cross sections are compared to selected theoretical calculations of the Standard Model expectations. No significant deviation from any of the tested predictions is found

Search for H→γγ produced in association with top quarks and constraints on the Yukawa coupling between the top quark and the Higgs boson using data taken at 7 TeV and 8 TeV with the ATLAS detector

A search is performed for Higgs bosons produced in association with top quarks using the diphoton decay mode of the Higgs boson. Selection requirements are optimized separately for leptonic and fully hadronic final states from the top quark decays. The dataset used corresponds to an integrated luminosity of 4.5 fb−14.5 fb−1 of proton–proton collisions at a center-of-mass energy of 7 TeV and 20.3 fb−1 at 8 TeV recorded by the ATLAS detector at the CERN Large Hadron Collider. No significant excess over the background prediction is observed and upper limits are set on the tt¯H production cross section. The observed exclusion upper limit at 95% confidence level is 6.7 times the predicted Standard Model cross section value. In addition, limits are set on the strength of the Yukawa coupling between the top quark and the Higgs boson, taking into account the dependence of the tt¯H and tH cross sections as well as the H→γγ branching fraction on the Yukawa coupling. Lower and upper limits at 95% confidence level are set at −1.3 and +8.0 times the Yukawa coupling strength in the Standard Model

Search for the Standard Model Higgs boson decay to μ+μ− with the ATLAS detector

A search is reported for Higgs boson decay to μ+μ−μ+μ− using data with an integrated luminosity of 24.8 fb−124.8 fb−¹ collected with the ATLAS detector in pp collisions at √s=7 and 8 TeV at the CERN Large Hadron Collider. The observed dimuon invariant mass distribution is consistent with the Standard Model background-only hypothesis in the 120–150 GeV search range. For a Higgs boson with a mass of 125.5 GeV, the observed (expected) upper limit at the 95% confidence level is 7.0 (7.2) times the Standard Model expectation. This corresponds to an upper limit on the branching ratio BR(H→μ+μ−)of 1.5×10−31.5×10−3

Managing potential interactions of subsurface resources

Subsurface resources include oil, gas, coal, groundwater, saline aquifer minerals, and heat (for geothermal use). Pore space itself should also be considered as a resource as it can be used for injection of waste fluids, produced water, storage of natural gas, compressed air, and supercritical CO₂. Use of subsurface resources can overlap in space, and pressure changes at one site can remotely influence resource use at other sites. Resource use can also vary in time, such as the use of depleted oil or gas fields for natural gas or CO₂ storage. Before allocation of a subsurface resource it is therefore useful to understand the potentially wide range of resources available in an area, how they might be developed successively, and how they could affect each other if used concurrently. While these issues are primarily geological, they have critical significance for legal, environmental, and economic considerations

Feasibility of storing carbon dioxide on a tectonically active margin: New Zealand

New Zealand\u27s sedimentary basins was found to have available several gigatonnes of CO2 storage capacity. However, CO2 storage is currently untested in New Zealand. The country\u27s position on an active Neogene plate boundary raises additional key factors that will influence final site selection. Some risk factors will also influence the relationship between social acceptance and the design of regulations. Despite the risks, hydrocarbon producing fields in Taranaki indicate that viable reservoir-seal pairs are likely to be present.Not peer reviewe