Effect of Pair Housing Versus Individual and Group Housing on Behavioural Patterns of Buffalo Calves

This study aimed to evaluate the effect of pair housing versus individual and group housing on behavioural patterns of female buffalo calves (n = 18; 15.0±3.0 days of age) were assigned randomly to 1 of 3 treatments of group housing with (C1=one calf/pen, C2=two calves/pen, or C3= three calves/pen) supplying a total pen space allowance of 1.82 m2 /calf, regardless of pen size. Behaviour was recorded by direct observation throughout the day from 10:00 to 14:00 clock, during a single day each week for 12 weeks using scan sampling every 15 min within 4 hours’ observation sessions. Calves housed in C2 group showed more (P ≤ 0.05) eating and drinking, chewing/ruminating, object manipulation and self grooming, lying activities, and less (P < 0.05) inactivity and standing when compared to calves housed in C1 and C3 groups. In conclusion, raising buffalo calves in paired housing system provided calves more opportunity to express their comfort, grooming, and feeding activities compared to individual and group housing system, however further investigation is still required to study the effect of paired housing system on the performance and physiological indicators in buffalo calves

Effects of Propolis, Ascorbic Acid and Vitamin E on Thyroid and Corticosterone Hormones in Heat Stressed Broilers

The study was designed to evaluate the effect of oxidative stress during the summer on serum levels of Triiodothyronine (T3), thyroxine (T4) and corticosterone hormones in Ross 308 broiler chickens and to compare the efficacy of propolis, ascorbic acid and vitamin E (α-tocopherol acetate) as antioxidants in amelioration of heat stress and normalization of serum T3 and T4 and corticosterone levels in broilers. Birds were divided into five groups during the experimental period (15 to 42 days of age), the positive control group (G1) was kept under thermo-neutral condition (28.0±4.0°C ) and fed control diet, while the other four groups were exposed to heat stress (38.0±1.4°C), control group (G2) chicks were fed control diet without additives, propolis group (G3) chicks were supplemented with 250 mg Chinese ether extracted propolis /kg diet, group (G4) supplemented with 250 mg ascorbic acid/kg diet and group (G5) supplemented with 250 mg of α-tocopherol acetate/kg diet (vitamin E). At the end of day 42, five broilers were randomly chosen from each treatment and slaughtered, and then sera were collected to estimate T3 and T4 and corticosterone levels. The results indicated that dietary supplementation of broilers with 250 mg/kg diet propolis, vitamin E or vitamin C can attenuate heat stress induced oxidative damage. These positive effects were evidenced by reduced serum corticosterone levels and improvement in thyroid hormonal levels in comparison to non-supplemented birds reared under heat stress

Theoretical and Experimental Sets of Choice Anode/Cathode Architectonics for High-Performance Full-Scale LIB Built-up Models

To control the power hierarchy design of lithium-ion battery (LIB) built-up sets for electric vehicles (EVs), we offer intensive theoretical and experimental sets of choice anode/cathode architectonics that can be modulated in full-scale LIB built-up models. As primary structural tectonics, heterogeneous composite superstructures of full-cell-LIB (anode//cathode) electrodes were designed in closely packed flower agave rosettes TiO2@C (FRTO@C anode) and vertical-star-tower LiFePO4@C (VST@C cathode) building blocks to regulate the electron/ion movement in the three-dimensional axes and orientation pathways. The superpower hierarchy surfaces and multi-directional orientation components may create isosurface potential electrodes with mobile electron movements, in-to-out interplay electron dominances, and electron/charge cloud distributions. This study is the first to evaluate the hotkeys of choice anode/cathode architectonics to assemble different LIB–electrode platforms with high-mobility electron/ion flows and high-performance capacity functionalities. Density functional theory calculation revealed that the FRTO@C anode and VST-(i)@C cathode architectonics are a superior choice for the configuration of full-scale LIB built-up models. The integrated FRTO@C//VST-(i)@C full-scale LIB retains a huge discharge capacity (~ 94.2%), an average Coulombic efficiency of 99.85% after 2000 cycles at 1 C, and a high energy density of 127 Wh kg−1, thereby satisfying scale-up commercial EV requirements

Simultaneous cognate epitope recognition by bovine CD4 and CD8 T cells is essential for primary expansion of antigen-specific cytotoxic T-cells following ex vivo stimulation with a candidate Mycobacterium avium subsp. paratuberculosis peptide vaccine

Studies in cattle show CD8 cytotoxic T cells (CTL), with the ability to kill intracellular bacteria, develop following stimulation of monocyte-depleted peripheral blood mononuclear cells (mdPBMC) with antigen-presenting cells (APC, i.e. conventional dendritic cells [cDC] and monocyte-derived DC [MoDC]) pulsed with MMP, a membrane protein from Mycobacterium avium subsp. paratuberculosis (Map) encoded byMAP2121c. CTL activity was diminished if CD4 T cells were depleted from mdPBMC before antigen (Ag) presentation by APC, suggesting simultaneous cognate recognition of MMP epitopes presented by MHC I and MHC II molecules to CD4 and CD8 T cells is essential for development of CTL activity. To explore this possibility, studies were conducted with mdPBMC cultures in the presence of monoclonal antibodies (mAbs) specific for MHC class I and MHC class II molecules. The CTL response of mdPBMC to MMP-pulsed APC was completely blocked in the presence of mAbs to both MHC I and II molecules and also blocked in the presence of mAbs to either MHC I or MHC II alone. The results demonstrate simultaneous cognate recognition of Ag by CD4 and CD8 T cells is essential for delivery of CD4 T cell help to CD8 T cells to elicit development of CTL

Effects of salicylic acid elicitor and potassium fertilizer as foliar spray on canola production in the reclaimed land in Ismailia governorate, Egypt

A field experiment was conducted at the farm of Faculty of Agriculture, Suez Canal University, Ismailia Governorate, Egypt, during 2013/’14 and 2014/’15 seasons, to determine the effects of salicylic acid elicitor (SA) and potassium fertilizer (K) as foliar spray on canola production in the reclaimed land. Canola plants were sprayed with three rates of K and SA separately and together. The concentrations of SA with a surfactant triton 0.1% and concentrations of K sprayed after 30 days of sowing by one week interval for three times using hydraulic sprayer. Results indicated that K and SA provided good nutrition and resistance for pathogens, enhanced plant height (cm), number of branches/ plant, fruiting zone (cm), seed yield/ plant (g), seed yield/ fed and oil percentage of canola cultivar (Serw 4) in the reclaimed land. K and SA separately or in combination at the rate of (6.0 cm-1 + 300 mg-1 SA) provided the best nutrition for enhancing resistance of plants against biotic and a biotic factors, consequently, enhancing vegetative growth and yield production during seasons of study 2013/’14 and 2014/’15

Asc-Dependent and Independent Mechanisms Contribute to Restriction of Legionella Pneumophila Infection in Murine Macrophages

The apoptosis-associated speck-like protein containing a caspase recruitment domain (Asc) is an adaptor molecule that mediates inflammatory and apoptotic signals. Legionella pneumophila is an intracellular bacterium and the causative agent of Legionnaire's pneumonia. L. pneumophila is able to cause pneumonia in immuno-compromised humans but not in most inbred mice. Murine macrophages that lack the ability to activate caspase-1, such as caspase-1−/− and Nlrc4−/− allow L. pneumophila infection. This permissiveness is attributed mainly to the lack of active caspase-1 and the absence of its down stream substrates such as caspase-7. However, the role of Asc in control of L. pneumophila infection in mice is unclear. Here we show that caspase-1 is moderately activated in Asc−/− macrophages and that this limited activation is required and sufficient to restrict L. pneumophila growth. Moreover, Asc-independent activation of caspase-1 requires bacterial flagellin and is mainly detected in cellular extracts but not in culture supernatants. We also demonstrate that the depletion of Asc from permissive macrophages enhances bacterial growth by promoting L. pneumophila-mediated activation of the NF-κB pathway and decreasing caspase-3 activation. Taken together, our data demonstrate that L. pneumophila infection in murine macrophages is controlled by several mechanisms: Asc-independent activation of caspase-1 and Asc-dependent regulation of NF-κB and caspase-3 activation

Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV

Results are presented from a search for a W' boson using a dataset corresponding to 5.0 inverse femtobarns of integrated luminosity collected during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV. The W' boson is modeled as a heavy W boson, but different scenarios for the couplings to fermions are considered, involving both left-handed and right-handed chiral projections of the fermions, as well as an arbitrary mixture of the two. The search is performed in the decay channel W' to t b, leading to a final state signature with a single lepton (e, mu), missing transverse energy, and jets, at least one of which is tagged as a b-jet. A W' boson that couples to fermions with the same coupling constant as the W, but to the right-handed rather than left-handed chiral projections, is excluded for masses below 1.85 TeV at the 95% confidence level. For the first time using LHC data, constraints on the W' gauge coupling for a set of left- and right-handed coupling combinations have been placed. These results represent a significant improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results