Bionomía del mosquito Aedes (Stegomyia) aegypti Ll.) En iglesias y cementerio de la ciudad de Mérida, Yucatán, México, y la identificación de arbovirus.

RESUMEN: Aedes aegypti es el vector principal de los virus dengue (VDEN), Zika (VZIK) y chikungunya (VCHIK). Aunque su bionomía es bien documentada para casas, su comportamiento es poco conocido en sitios públicos. En el presente estudio, la bionomía de Ae. aegypti fue descrito en iglesias y cementerio de la ciudad de Mérida, Yucatán. Los sitios de estudio fueron visitados cuatro veces por mes entre septiembre de 2015 y octubre de 2016. Los mosquitos adultos, fueron capturados con aspiradores de mochila. Las larvas y pupas fueron colectadas con la ayuda de pipetas de plástico. La duración del ciclo gonotrófico fue estimado con el análisis de correlación cruzada, basado en la paridad diaria de Ae. aegypti durante 25 días. La preferencia alimenticia del mosquito fue determinada con el gen del citocromo b de humanos y mamíferos. Los arbovirus fueron identificados con RT-PCR mediante la amplificación de regiones específicas de los genes de VDEN, VZIK y VCHIK. Durante todo el periodo de estudio, en las iglesias se colectaron 6,051 larvas y 315 pupas. Mientras que, en el cementerio se colectaron 8,052 larvas y 575 pupas. En cuanto los mosquitos adultos de Ae. aegypti, se colectaron en iglesias 1,380 hembras. De las cuales, 1,178 en la estación de lluvias y 202 en la de seca; la mayoría estaban alimentadas (615). En el cementerio se obtuvieron 1,648 hembras con 1,471 en periodo de lluvias y solo 177 en el de secas; la mayoría estaban sin alimentar (823). En ambos sitios de estudio, la duración del ciclo gonotrófico fue de cuatro días en temporada de lluvia y tres días en la temporada de secas. El 76% (223/292) de hembras de Ae. aegypti con alimentación reciente fueron analizadas y todas fueron identificadas con sangre de humano. En las iglesias fueron identificados dos pools de Ae. aegypti infectadas con el virus dengue (DEN-1 y 2). En el cementerio, un pool de Ae. aegypti fue infectado con dengue (DEN-1) y otro con el virus Zika. Los resultados sugieren que las iglesias y el cementerio de la ciudad de Mérida, Yucatán representan riesgo potencial en la transmisión de arbovirus. ABSTRACT: Aedes aegypti is the main vector of the dengue, Zika and chikungunya viruses. Although its bionomy is well documented in houses, their behavior is little known in public places. In the present study, the bionomy of Ae. aegypti was described in churches and cemetery of the Mérida city, Yucatán. Field work was undertaken from September 2015 to October 2016 and sampling was conducted four times per month. Resting mosquitoes were collected by using backpack-mounted aspirators. Nets, turkey basters, or pipettes were used to collect immatures from water-holding containers. Daily changes in the parous–nulliparous ratio was recorded for 25 days, and the gonotrophic cycle length was estimated by a time series analysis and the source of bloodmeal of Ae. aegypti. The host preference of Ae. aegypti was determined with the human and mammalian cytochrome b gene. Aedes aegypti females were processed for VDEN, VZIK and VCHIK identification by RT-PCR. For the entire study, 6,051 larvae and 315 pupae were collected in churches. In cemetery, 8,052 larvae and 575 pupae were collected. Of the Ae. aegypti adults, 1,380 females were collected in churches. Of which, 1,178 in the rainy season and 202 in the dry season; most were fed (615). In cemetery, 1,648 females were collected, with 1,471 in the rainy season and 177 in the dry season; most were unfed (823). In both study sites, the duration of the gonotrophic cycle was four and three days in rainy and dry season, respectively. For source of bloodmeal, we processed 76% (223/292) of Ae. aegypti females with freshly blood and all females tested were identified with human blood. In churches, two positive pools to dengue virus (DENV-1 and DENV-2) were identified. In cemetery, one pool was positive a DEN-1 and another to Zika virus. The results suggest that churches and cemetery represents potential risk of arbovirus transmission in Merida city

Complete Genome Sequence of Houston Virus, a Newly Discovered Mosquito-Specific Virus Isolated from Culex quinquefasciatus in Mexico

ABSTRACT We fully sequenced the genome of Houston virus, a recently discovered mosquito-associated virus belonging to the newly established family Mesoniviridae. The isolate was recovered from Culex quinquefasciatus in southern Mexico, which shows that the geographic range of Houston virus is not restricted to the United States in North America

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Search for supersymmetry at √s=13 TeV in final states with jets and two same-sign leptons or three leptons with the ATLAS detector

A search for strongly produced supersymmetric particles is conducted using signatures involving multiple energetic jets and either two isolated leptons (e or μ μ) with the same electric charge or at least three isolated leptons. The search also utilises b-tagged jets, missing transverse momentum and other observables to extend its sensitivity. The analysis uses a data sample of proton–proton collisions at √s=13 TeV recorded with the ATLAS detector at the Large Hadron Collider in 2015 corresponding to a total integrated luminosity of 3.2 fb −1. No significant excess over the Standard Model expectation is observed. The results are interpreted in several simplified supersymmetric models and extend the exclusion limits from previous searches. In the context of exclusive production and simplified decay modes, gluino masses are excluded at 95% 95% confidence level up to 1.1–1.3 TeV for light neutralinos (depending on the decay channel), and bottom squark masses are also excluded up to 540 GeV. In the former scenarios, neutralino masses are also excluded up to 550–850 GeV for gluino masses around 1 TeV

Search for supersymmetry in events with large missing transverse momentum, jets, and at least one tau lepton in 20 fb−1 of √s=8 TeV proton-proton collision data with the ATLAS detector

A search for supersymmetry (SUSY) in events with large missing transverse momentum, jets, at least one hadronically decaying tau lepton and zero or one additional light leptons (electron/muon), has been performed using 20.3fb−1 of proton-proton collision data at √s= 8 TeV recorded with the ATLAS detector at the Large Hadron Collider. No excess above the Standard Model background expectation is observed in the various signal regions and 95% confidence level upper limits on the visible cross section for new phenomena are set. The results of the analysis are interpreted in several SUSY scenarios, significantly extending previous limits obtained in the same final states. In the framework of minimal gauge-mediated SUSY breaking models, values of the SUSY breaking scale Λ below 63 TeV are excluded, independently of tan β. Exclusion limits are also derived for an mSUGRA/CMSSM model, in both the R-parity-conserving and R-parity-violating case. A further interpretation is presented in a framework of natural gauge mediation, in which the gluino is assumed to be the only light coloured sparticle and gluino masses below 1090 GeV are excluded

Search for direct top-squark pair production in final states with two leptons in pp collisions at √s = 8TeV with the ATLAS detector

A search is presented for direct top-squark pair production in final states with two leptons (electrons or muons) of opposite charge using 20.3 fb−1 of pp collision data at √s = 8 TeV, collected by the ATLAS experiment at the Large Hadron Collider in 2012. No excess over the Standard Model expectation is found. The results are interpreted under the separate assumptions (i) that the top squark decays to a b-quark in addition to an on-shell chargino whose decay occurs via a real or virtual W boson, or (ii) that the top squark decays to a t-quark and the lightest neutralino. A top squark with a mass between 150 GeV and 445 GeV decaying to a b-quark and an on-shell chargino is excluded at 95% confidence level for a top squark mass equal to the chargino mass plus 10 GeV, in the case of a 1 GeV lightest neutralino. Top squarks with masses between 215 (90) GeV and 530 (170) GeV decaying to an on-shell (off-shell) t-quark and a neutralino are excluded at 95% confidence level for a 1 GeV neutralino

Search for direct top squark pair production in events with a Z boson, b-jets and missing transverse momentum in s √ = 8 TeV pp collisions with the ATLAS detector

A search is presented for direct top squark pair production using events with at least two leptons including a same-flavour opposite-sign pair with invariant mass consistent with the Z boson mass, jets tagged as originating from b-quarks and missing transverse momentum. The analysis is performed with proton–proton collision data at s√=8 TeV collected with the ATLAS detector at the LHC in 2012 corresponding to an integrated luminosity of 20.3 fb−¹. No excess beyond the Standard Model expectation is observed. Interpretations of the results are provided in models based on the direct pair production of the heavier top squark state (t~2) followed by the decay to the lighter top squark state (t~1) via t~2→Zt~1, and for t~1 pair production in natural gauge-mediated supersymmetry breaking scenarios where the neutralino (χ~10) is the next-to-lightest supersymmetric particle and decays producing a Z boson and a gravitino (G~) via the χ~10→ZG~ process

Search for top squark pair production in final states with one isolated lepton, jets, and missing transverse momentum in √s = 8 TeV pp collisions with the ATLAS detector

The results of a search for top squark (stop) pair production in final states with one isolated lepton, jets, and missing transverse momentum are reported. The analysis is performed with proton-proton collision data at s√ = 8 TeV collected with the ATLAS detector at the LHC in 2012 corresponding to an integrated luminosity of 20 fb−1. The lightest supersymmetric particle (LSP) is taken to be the lightest neutralino which only interacts weakly and is assumed to be stable. The stop decay modes considered are those to a top quark and the LSP as well as to a bottom quark and the lightest chargino, where the chargino decays to the LSP by emitting a W boson. A wide range of scenarios with different mass splittings between the stop, the lightest neutralino and the lightest chargino are considered, including cases where the W bosons or the top quarks are off-shell. Decay modes involving the heavier charginos and neutralinos are addressed using a set of phenomenological models of supersymmetry. No significant excess over the Standard Model prediction is observed. A stop with a mass between 210 and 640 GeV decaying directly to a top quark and a massless LSP is excluded at 95% confidence level, and in models where the mass of the lightest chargino is twice that of the LSP, stops are excluded at 95% confidence level up to a mass of 500 GeV for an LSP mass in the range of 100 to 150 GeV. Stringent exclusion limits are also derived for all other stop decay modes considered, and model-independent upper limits are set on the visible cross-section for processes beyond the Standard Model

Defining the causes of sporadic Parkinson's disease in the global Parkinson's genetics program (GP2)

The Global Parkinson’s Genetics Program (GP2) will genotype over 150,000 participants from around the world, and integrate genetic and clinical data for use in large-scale analyses to dramatically expand our understanding of the genetic architecture of PD. This report details the workflow for cohort integration into the complex arm of GP2, and together with our outline of the monogenic hub in a companion paper, provides a generalizable blueprint for establishing large scale collaborative research consortia