The pp-Daugavet property for function spaces

A natural extension of the Daugavet property for $p$-convex Banach function spaces and related classes is analysed. As an application, we extend the arguments given in the setting of the Daugavet property to show that no reflexive space falls into this class

Characterization of new cell line stably expressing CHI3L1 oncogene

Aim. To characterize the immortalized 293 cell line afterstable transfection with human oncogene (CHI3L1). Methods. 293 cells, stably transfected with pcDNA3.1_CHI3L1, and 293 cells, stably transfected with pcDNA3.1 as a negative control, were used throughout all experiments. The clones of CHI3L1-expressing 293 cells and 293 cells, transfected with pcDNA3.1, were analyzed by immunofluorescence and confocal microscopy. Cell proliferation was measured using MTT assay; analyses of ERK1/2 and AKT activation and their cellular localization were performed with anti-phospho-ERK and anti-phospho-AKT antibodies. Specific activation of MAP and PI3 kinases was measured by densitometric analysis of Western-blot signals. Results. The obtained results show quite modest ability of CHI3L1 to stimulate cell growth and reflect rather an improved cellular plating efficiency of the 293 cells stably transfected with pcDNA3.1_CHI3L1 as compared to the 293 cells transfected with an «empty» vector. ERK1/2 and AKT are activated in the 293_CHI3L1 cells. In these cells phosphorylated ERK1/2 were localized in both cell cytoplasm and nuclei while AKT only in cytoplasm. The 293_CHI3L1 cells differed from the 293 cells, transfected with an «empty» vector, in their size and ability to adhere to the culture plates. Conclusions. The overexpression of CHI3L1 is likely to have an important role in tumorigenesis via a mechanism which involves activation of PI3K and ERK1/2 pathways. The tumors which can be induced by orthotopic implantation of the transformed human cells with overexpressed human oncogene CHI3L1 into the rat brain can be used as a target for anticancer drug development. Key words: chitinase 3-like 1 protein (CHI3L1), brain tumor, MAP kinase, PI3 kinase.Мета. Охарактеризувати імморталізовану клітинну лінію 293 після стабільної трансфекції онкогена CHI3L1. Методи. Клітини 293, стабільно трансфековані pcDNA3.1_CHI3L1, та клітини 293, стабільно трансфековані pcDNA3.1 як негативний контроль, використано в усіх експериментах. Клони 293 експресуючих CHI3L1 клітин та клітини 293, трансфековані «порожнім» вектором, проаналізовано методами імунофлуоресценції та конфокальної мікроскопії. Клітинну проліферацію визначено за допомогою MTT, активацію ERK1/2 i AKT та їxню локалізацію в клітинax – із застосуванням анти-фосфо-ERK- та анти-фосфо-AKT-антитіл. Специфічну активацію кіназ MAP і PI3 визначено денситометричним аналізом Вестерн-блот сигналів. Результати. Отримані результати демонструють помірну здатність CHI3L1 стимулювати клітинний ріст, алe відображають скоріше підвищену здатність до прикріплення 293 клітин, стабільно трансфекованих pcDNA3.1_CHI3L1 порівняно з клітинами 293, трансфекованими «порожнім» вектором. У 293_ CHI3L1 клітинах ERK1/2 та AKT перебувають в активованому стані. У цих клітинах фосфорильованi ERK1/2 локалізованi як у цитоплазмі, так і в ядрі, тоді як AKT – лише в цитоплазмі. 293_CHI3L1-клітини відрізняються від клітин 293_pcDNA3.1 за морфологією та їхньою здатністю до прикріплення до культуральних чашок. Висновки. Нaдeкспресія CHI3L1, очевидно, має важливе значення в пухлиноутворунні і опоседковується активацією PI3K- і MAPK- сигнальних шляхів. Пухлини, спричинені ортотопічною імплантацією трансформованих клітин людини з нaдекспресованимCHI3L1 у мозок щурів, стають вірогідною мішенню для антиракової терапії. Ключові слова: хітиназа 3-подібний білок 1 (CHI3L1), пухлини гoлoвного мозку, MAP-кіназа, PI3-кіназа.Цель. Охарактеризовать иммортализованную клеточную линию 293 после стабильной трансфекции онкогена CHI3L1. Методы. Клетки 293, стабильно трансфецированные pcDNA3.1_CHI3L1, и клетки 293, стабильно трансфецированные pcDNA3.1 в качестве отрицательного контроля, использованы во всех экспериментах. Клоны 293_CHI3L1 и клетки 293_ pcDNA3.1 анализировали методами иммунофлюоресценции и конфокальной микроскопии. Клеточную пролиферацию определяли с помощью MTT, активацию и локализацию ERK1/2 и AKT анализировали с применением анти-фосфо-ERK- и анти-фосфо-AKT-антител. Специфическую активацию MAP- и PI3-киназ определяли денситометрическим анализом Вестерн-блот сигналов. Результаты. Полученные ре - зультаты демонстрируют умеренную способность CHI3L1 стимулировать клеточный рост и отражают скорее повышенную способность к прикреплению клеток, стабильно трансфецированных pcDNA3.1_CHI3L1 по сравнению с 293-клетками, транс - фецированными «пустым» вектором. ERK1/2 и AKT в клетках 293_CHI3L1 находятся в активированном состоянии. В этих клетках фосфорилированныe ERK1/2 локализованы как в цитоплазме, так и в ядре, в то время как AKT – только в цитоплазме. Клетки 293_CHI3L1 отличаются от клеток 293_pcDNA3.1 по морфологии и способности прикрепления к культуральным чашкам. Выводы. Сверхэкспрессия CHI3L1, очевидно, имеет важное значение в опухолеобразовании и опосредуется активацией PI3Kи MAPK-сигнальных путей. Опухоли, которые индуцируются ортотопической имплантацией трансформированных человеческих клеток со сверхэкспрессированным CHI3L1 в мозг крыс, мо - гут служить мишенью для антираковой терапии. Ключевые слова: хитиназа 3-подобный белок 1 (CHI3L1), опухоли гoлoвного мозга, MAP-киназа, PI3-киназ

Expression of genes belonging to the IGF-system in glial tumors

The discrepancies arising from conflicting evidence on the results obtained by different laboratories in human gliomas are discussed. Our data highlight the importance of viewing the IGF-related proteins as a complex multifactorial system and show that changes in the expression levels of any one component of the system, in a given malignancy, should be interpreted with caution. As IGF targeting for anticancer therapy is rapidly becoming clinical reality, an understanding of this complexity is very timely.B cтaтьe oбсуждаются противоречивыe результаты, oпиcaнныe различными лабораториями для глиом. Пoлучeнныe данные демонстрируют важность рассмотрения белков семейства инсулиноподобных факторов роста как сложную мультифункциональную систему и показывают, что изменения в уровне экспрессии любого компонента системы в упомянутой опухоли должны интерпретироваться с предосторожностью. В связи с тем, что выбор членoв IGF-ceмeйcтвa в качестве мишени для противоопухолевой терапии быстро приобретает клиническую реальность, понимание сложноcти этой системы является весьма своевременным.У cтaттi oбговорюються суперечливi результати, опиcанi різними лабораторіями для гліом. Oтриманi дані демонструють важливість розгляду білків родини інсуліноподібних факторів росту як складну мультифункціональну систему і показують, що зміни рівня експресії будь-якого компонента системи у даній пухлині повинні інтерпретуватися із пересторогою. В зв’язку з тим, що вибір членiв IGF-ciмeйcтвa як мішені для протипухлинної терапії швидко набуває клінічної реальності, розуміння цієї системи є вельми своєчасним

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters

Measurement of the inclusive isolated prompt photon cross-section in pp collisions at sqrt(s)= 7 TeV using 35 pb-1 of ATLAS data

A measurement of the differential cross-section for the inclusive production of isolated prompt photons in pp collisions at a center-of-mass energy sqrt(s) = 7 TeV is presented. The measurement covers the pseudorapidity ranges |eta|<1.37 and 1.52<=|eta|<2.37 in the transverse energy range 45<=E_T<400GeV. The results are based on an integrated luminosity of 35 pb-1, collected with the ATLAS detector at the LHC. The yields of the signal photons are measured using a data-driven technique, based on the observed distribution of the hadronic energy in a narrow cone around the photon candidate and the photon selection criteria. The results are compared with next-to-leading order perturbative QCD calculations and found to be in good agreement over four orders of magnitude in cross-section.Comment: 7 pages plus author list (18 pages total), 2 figures, 4 tables, final version published in Physics Letters

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02

Measurement of D*+/- meson production in jets from pp collisions at sqrt(s) = 7 TeV with the ATLAS detector

This paper reports a measurement of D*+/- meson production in jets from proton-proton collisions at a center-of-mass energy of sqrt(s) = 7 TeV at the CERN Large Hadron Collider. The measurement is based on a data sample recorded with the ATLAS detector with an integrated luminosity of 0.30 pb^-1 for jets with transverse momentum between 25 and 70 GeV in the pseudorapidity range |eta| < 2.5. D*+/- mesons found in jets are fully reconstructed in the decay chain: D*+ -> D0pi+, D0 -> K-pi+, and its charge conjugate. The production rate is found to be N(D*+/-)/N(jet) = 0.025 +/- 0.001(stat.) +/- 0.004(syst.) for D*+/- mesons that carry a fraction z of the jet momentum in the range 0.3 < z < 1. Monte Carlo predictions fail to describe the data at small values of z, and this is most marked at low jet transverse momentum.Comment: 10 pages plus author list (22 pages total), 5 figures, 1 table, matches published version in Physical Review